Pengaruh Pola Asuh Orang Tua Terhadap Perkembangan Emosi Anak Di TK Desa Juwangi Kecamatan Juwangi Kabupaten Boyolali Tahun Ajaran 2013/2014

Tujuan penelitian ini adalah untuk mengetahui pengaruh pola asuh terhadap perkembangan emosi anak di TK Desa Juwangi, Kecamatan Juwangi, Kabupaten Boyolali Tahun Pelajaran 2013/2014. Penelitian ini merupakan penelitian deskriptif korelasional. Populasinya yaitu seluruh anak TK B yang ada di desa Juwangi Kab. Boyolali, yaitu TK Tunas Rimba, TK Siti Barokah, TK Darmawanita berjumlah 140 anak. Sampel sebanyak 35 anak. Teknik sampling dalam penelitian ini menggunakan teknik proporsional random sampling. Adapun metode pengumpulan data yang digunakan adalah observasi dan angket. Teknik analisis data menggunakan teknik analisis regresi liner sederhana, uji t dan koefisien determinasi. Berdasarkan hasil penelitian dapat disimpulkan bahwa pengaruh antara pola asuh demokratis terhadap perkembangan emosi anak dapat dilihat dari uji regresi linier sederhana sebesar 1,265. Besarnya pengaruh pola asuh demokratis terhadap perkembangan emosi anak dapat dilihat dari nilai R Square yaitu sebesar 0,466 atau 46,6% yang berarti variasi perubahan variabel perkembangan emosi anak dipengaruhi oleh variabel pola asuh sebesar 0,466 atau 46,6%. Sedangkan sisanya 53,4% (100-46,6 = 53,4) dijelaskan oleh variabel lain di luar model yang tidak diteliti

Pathogenic Huntingtin Repeat Expansions in Patients with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis

Hannu Laaksovirta konsortion jäsenenä.The Genomics England Research Consortium, The International ALS/FTD Genomics Consortium (iAFGC), The International FTD Genetics Consortium (IFGC), The International LBD Genomics Consortium (iLBDGC), The NYGC ALS Consortium, The PROSPECT Consortium,17 James B. Rowe,17 Luisa Benussi,18 Giuliano Binetti,18,19 Roberta Ghidoni,18 Edwin Jabbari,20,21 Coralie Viollet,22 Jonathan D. Glass,23 Andrew B. Singleton,24 Vincenzo Silani,25,26 Owen A. Ross,27 Mina Ryten,8,28,29 Ali Torkamani,30 Toshiko Tanaka,31 Luigi Ferrucci,31 Susan M. Resnick,32 We examined the role of repeat expansions in the pathogenesis of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) by analyzing whole-genome sequence data from 2,442 FTD/ALS patients, 2,599 Lewy body dementia (LBD) patients, and 3,158 neurologically healthy subjects. Pathogenic expansions (range, 40?64 CAG repeats) in the huntingtin (HTT) gene were found in three (0.12%) patients diagnosed with pure FTD/ALS syndromes but were not present in the LBD or healthy cohorts. We replicated our findings in an independent collection of 3,674 FTD/ALS patients. Postmortem evaluations of two patients revealed the classical TDP-43 pathology of FTD/ALS, as well as huntingtin-positive, ubiquitin-positive aggregates in the frontal cortex. The neostriatal atrophy that pathologically defines Huntington?s disease was absent in both cases. Our findings reveal an etiological relationship between HTT repeat expansions and FTD/ALS syndromes and indicate that genetic screening of FTD/ALS patients for HTT repeat expansions should be considered. We examined the role of repeat expansions in the pathogenesis of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) by analyzing whole-genome sequence data from 2,442 FTD/ALS patients, 2,599 Lewy body dementia (LBD) patients, and 3,158 neurologically healthy subjects. Pathogenic expansions (range, 40?64 CAG repeats) in the huntingtin (HTT) gene were found in three (0.12%) patients diagnosed with pure FTD/ALS syndromes but were not present in the LBD or healthy cohorts. We replicated our findings in an independent collection of 3,674 FTD/ALS patients. Postmortem evaluations of two patients revealed the classical TDP-43 pathology of FTD/ALS, as well as huntingtin-positive, ubiquitin-positive aggregates in the frontal cortex. The neostriatal atrophy that pathologically defines Huntington?s disease was absent in both cases. Our findings reveal an etiological relationship between HTT repeat expansions and FTD/ALS syndromes and indicate that genetic screening of FTD/ALS patients for HTT repeat expansions should be considered.Peer reviewe

Pathogenic Huntingtin Repeat Expansions in Patients with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis.

We examined the role of repeat expansions in the pathogenesis of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) by analyzing whole-genome sequence data from 2,442 FTD/ALS patients, 2,599 Lewy body dementia (LBD) patients, and 3,158 neurologically healthy subjects. Pathogenic expansions (range, 40-64 CAG repeats) in the huntingtin (HTT) gene were found in three (0.12%) patients diagnosed with pure FTD/ALS syndromes but were not present in the LBD or healthy cohorts. We replicated our findings in an independent collection of 3,674 FTD/ALS patients. Postmortem evaluations of two patients revealed the classical TDP-43 pathology of FTD/ALS, as well as huntingtin-positive, ubiquitin-positive aggregates in the frontal cortex. The neostriatal atrophy that pathologically defines Huntington's disease was absent in both cases. Our findings reveal an etiological relationship between HTT repeat expansions and FTD/ALS syndromes and indicate that genetic screening of FTD/ALS patients for HTT repeat expansions should be considered

Genome-wide structural variant analysis identifies risk loci for non-Alzheimer’s dementias

We characterized the role of structural variants, a largely unexplored type of genetic variation, in two non-Alzheimer’s dementias, namely Lewy body dementia (LBD) and frontotemporal dementia (FTD)/amyotrophic lateral sclerosis (ALS). To do this, we applied an advanced structural variant calling pipeline (GATK-SV) to short-read whole-genome sequence data from 5,213 European-ancestry cases and 4,132 controls. We discovered, replicated, and validated a deletion in TPCN1 as a novel risk locus for LBD and detected the known structural variants at the C9orf72 and MAPT loci as associated with FTD/ALS. We also identified rare pathogenic structural variants in both LBD and FTD/ALS. Finally, we assembled a catalog of structural variants that can be mined for new insights into the pathogenesis of these understudied forms of dementia

Pemaknaan Ilustrasi Berita Infografis pada Media Online : Analisis Semiotika pada Instagram CNBC Indonesia

Penelitian ini bertujuan untuk mengetahui makna dari ilustrasi berita infografis pada media online instagram CNBC Indonesia melalui analisis semiotik Roland Barthes yaitu makna dari denotasi, konotasi dan mitos yang terkandung didalam ilustrasi berita infografis. Penelitian ini menggunakan penelitian kualitatif dengan paradigma konstruktivisme. Teknik pengumpulan data dilakukan dengan cara dokumentasi, wawancara dan observasi terhadap ilustator atau pembuat ilustrasi dari berita yang akan dilakukan penelitian. Hasil penelitian menunjukan bahwa dalam setiap ilustrasi pada berita infografis CNBC Indonesia melalui Instagram akan selalu memiliki makna denotasi di dalamnya. Dari penelitian tersebut dapat disimpulkan untuk memahami sebuah ilustrasi kita dianjurkan untuk lebih melihat aspek denotasi dan konotasi dengan mitos sebagai pendamping

Hubungan Ketepatan Pemilihan Antibiotik Empiris dengan Outcome Terapi pada Pasien Sepsis Di Instalasi Rawat Inap Beberapa Rumah Sakit

Terapi antibiotik merupakan salah satu komponen penunjang dalam keberhasilan pengobatan sepsis. Salah satu terapi sepsis yaitu menggunakan antibiotik empiris. Antibiotik empiris yang digunakan  harus rasional, adekuat dan tepat untuk menghindari terjadinya resistensi. Tujuan penelitian ini adalah mengetahui karakteristik pasien, pola pemilihan antibiotik empiris, pola sensitivitas dan resistensi bakteri terhadap antibiotik, ketepatan pemilihan antibiotik empiris, dan hubungan ketepatan pemilihan antibioktik empiris dengan outcome terapi. Penelitian ini adalah penelitian observasional dengan cara pengambilan data secara retrospektif, kemudian dianalisis secara deskriptif, dengan kriteria inklusi adalah diagnosa sepsis, usia dewasa, dirawat inap rumah sakit Abdul Wahab Sjahranie Samarinda dan A.M Parikesit Tenggarong periode Januari-Desember 2017. Hasil penelitian menunjukkan antibiotik empiris yang digunakan adalah Ceftriaxone nilai sensitivitas sebesar 26,67% dan Meropenem nilai sensitivitas sebesar 16,67%. Ketepatan antibiotik berdasarkan uji sensitivitas bakteri menunjukkan persentase ketepatan sebesar 61,90%. Hasil analisis statistik menunjukkan bahwa adanya hubungan ketepatan pemilihan antibiotik empiris pada pasien sepsis dengan outcome terapi  (p < 0,05)

Conceptualizing Green Education Awareness in Primary School to Promote Sustainability

This research aims to make environmental awareness and action an intrinsic part of life at school. This should include the students, teachers, non-teaching staff, and parents, as well as the Local Authority, the media, and local business. Green education endeavors to extend learning beyond the classroom and develop responsible attitudes and commitment. Schools consume enormous quantities of paper and energy, produce tons of waste and carbon emissions, and rarely purchase environmentally friendly products. Schools use cleaners and pesticides with neurological and reproductive toxins, which are dangerous. Poor indoor air quality and nutrition at school are linked to soaring asthma and childhood obesity rates. Research now shows that greener, sustainable school environments can save money and resources, expand learning, and improve health. And ultimately, sustainable schools teach children to become good environmental citizens and will empower them to make a difference in the environment

Structural enzymology binding studies of the peptide‐substrate‐binding domain of human collagen prolyl 4‐hydroxylase (type‐II):high affinity peptides have a PxGP sequence motif

Abstract The peptide‐substrate‐binding (PSB) domain of collagen prolyl 4‐hydroxylase (C‐P4H, an α2β2 tetramer) binds proline‐rich procollagen peptides. This helical domain (the middle domain of the α subunit) has an important role concerning the substrate binding properties of C‐P4H, although it is not known how the PSB domain influences the hydroxylation properties of the catalytic domain (the C‐terminal domain of the α subunit). The crystal structures of the PSB domain of the human C‐P4H isoform II (PSB‐II) complexed with and without various short proline‐rich peptides are described. The comparison with the previously determined PSB‐I peptide complex structures shows that the C‐P4H‐I substrate peptide (PPG)3, has at most very weak affinity for PSB‐II, although it binds with high affinity to PSB‐I. The replacement of the middle PPG triplet of (PPG)3 to the nonhydroxylatable PAG, PRG, or PEG triplet, increases greatly the affinity of PSB‐II for these peptides, leading to a deeper mode of binding, as compared to the previously determined PSB‐I peptide complexes. In these PSB‐II complexes, the two peptidyl prolines of its central P(A/R/E)GP region bind in the Pro5 and Pro8 binding pockets of the PSB peptide‐binding groove, and direct hydrogen bonds are formed between the peptide and the side chains of the highly conserved residues Tyr158, Arg223, and Asn227, replacing water mediated interactions in the corresponding PSB‐I complex. These results suggest that PxGP (where x is not a proline) is the common motif of proline‐rich peptide sequences that bind with high affinity to PSB‐II

Crystal structure of the collagen prolyl 4-hydroxylase (C-P4H) catalytic domain complexed with PDI: Toward a model of the C-P4H α2β2α_2 β_2 tetramer

Collagen prolyl 4-hydroxylases (C-P4H) are $α_2 β_2$ tetramers, which catalyze the prolyl 4-hydroxylation of procollagen, allowing for the formation of the stable triple-helical collagen structure in the endoplasmic reticulum. The C-P4H $α$-subunit provides the N-terminal dimerization domain, the middle peptide-substrate-binding (PSB) domain, and the C-terminal catalytic (CAT) domain, whereas the $β$-subunit is identical to the enzyme protein disulfide isomerase (PDI). The structure of the N-terminal part of the $α$-subunit (N-terminal region and PSB domain) is known, but the structures of the PSB-CAT linker region and the CAT domain as well as its mode of assembly with the $β$/PDI subunit, are unknown. Here, we report the crystal structure of the CAT domain of human C-P4H-II complexed with the intact $β$/PDI subunit, at 3.8 Å resolution. The CAT domain interacts with the a, b’, and a’ domains of the $β$/PDI subunit, such that the CAT active site is facing bulk solvent. The structure also shows that the C-P4H-II CAT domain has a unique N-terminal extension, consisting of α-helices and a $β$-strand, which is the edge strand of its major antiparallel $β$-sheet. This extra region of the CAT domain interacts tightly with the $β$/PDI subunit, showing that the CAT-PDI interface includes an intersubunit disulfide bridge with the a’ domain and tight hydrophobic interactions with the b’ domain. Using this new information, the structure of the mature C-P4H-II $α_2 β_2$ tetramer is predicted. The model suggests that the CAT active-site properties are modulated by $α$-helices of the N-terminal dimerization domains of both subunits of the $α_2$-dimer