Orthopedic Screwdriver Design

Design an adjustable mechanical clutch screwdriver suitable for driving Orthopedic Screws into bones. The clutch would prevent over tightening and damaging bone material. The prototype design must demonstrate the function and ergonomics of the device and it must be suitable for modification into a medical device. Specify material changes and design details that would be needed for the ultimate production model

4-({[(E)-Pyridin-3-yl­methyl­idene]amino}­meth­yl)cyclo­hexa­necarb­oxy­lic acid

The title compound, C14H18N2O2, contains two geometrically different mol­ecules in the asymmetric unit: the basal plane of the cyclo­hexane chair and the N-[pyridin-3-yl­methyl­idene]methanamine moiety are oriented at dihedral angles of 71.77 (7)° and 83.42 (8)°. In the crystal, the mol­ecules are linked by O—H⋯N hydrogen bonds, generating C(13) head-to-tail chains extending along the base vector [103]. R 2 2(26) ring motifs are formed due to the C—H⋯·O inter­actions that link neighbouring chains. There also exist π–π inter­actions [centroid–centroid separation = 3.6925 (12) Å] between the symmetry-related pyridine rings of one of the independent mol­ecules

ANTIMICROBIAL SCREENING OF SELECTED FLORA OF PAKISTAN

Abstract -ethanolic extracts of Ferula assafoetida resin, Grewia asiatica leaves, Ipomoea hederacea seeds, Lepidium sativum seeds, Nigella sativa seeds and Terminalia chebula fruits were tested in vitro for their antibacterial and antifungal activities. The antibacterial study performed against eight bacterial species viz., Escherichia coli, Citrobacter, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, Micrococcus luteus, Proteus mirabilis and Bacillus subtilis indicated that the investigated plants have potent activity against all the tested microorganisms. The antifungal activity of these extracts was performed against nine fungal strains, viz., Aspergillus parasiticus, Aspergillus niger, Yersinia aldovae, Candida albicans, Aspergillus effusus, Fusarium solani, Macrophomina phaseolina, Saccharomyces cerevisiae and Trichophyton rubrum. The extracts showed moderate as well as significant activity against the different fungal strains

Designer covalent heterobivalent inhibitors prevent IgE-dependent responses to peanut allergen

Allergies are a result of allergen proteins cross-linking allergen-specific IgE (sIgE) on the surface of mast cells and basophils. The diversity and complexity of allergen epitopes, and high-affinity of the sIgE-allergen interaction have impaired the development of allergen-specific inhibitors of allergic responses. This study presents a design of food allergen-specific sIgE inhibitors named covalent heterobivalent inhibitors (cHBIs) that selectively form covalent bonds to only sIgEs, thereby permanently inhibiting them. Using screening reagents termed nanoallergens, we identified two immunodominant epitopes in peanuts that were common in a population of 16 allergic patients. Two cHBIs designed to inhibit only these two epitopes completely abrogated the allergic response in 14 of the 16 patients in an in vitro assay and inhibited basophil activation in an allergic patient ex vivo analysis. The efficacy of the cHBI design has valuable clinical implications for many allergen-specific responses and more broadly for any antibody-based disease

Can an ethics code help to achieve equity in international research collaborations? Implementing the Global Code of Conduct for Research in Resource-Poor Settings in India and Pakistan.

The Global Code of Conduct for Research in Resource-Poor Settings (GCC) aims to stop the export of unethical research practices from higher to lower income settings. Launched in 2018, the GCC was immediately adopted by European Commission funding streams for application in research that is situated in lower and lower-middle income countries. Other institutions soon followed suit. This article reports on the application of the GCC in two of the first UK-funded projects to implement this new code, one situated in India and one in Pakistan. Through systematic ethics evaluation of both projects, the practical application of the GCC in real-world environments was tested. The findings of this ethics evaluation suggest that while there are challenges for implementation, application of the GCC can promote equity in international research collaborations

Search-based inference of polynomial metamorphic relations

Metamorphic testing (MT) is an effective methodology for testing those so-called 'non-testable' programs (e.g., scientific programs), where it is sometimes very difficult for testers to know whether the outputs are correct. In metamorphic testing, metamorphic relations (MRs) (which specify how particular changes to the input of the program under test would change the output) play an essential role. However, testers may typically have to obtain MRs manually. In this paper, we propose a search-based approach to automatic inference of polynomial MRs for a program under test. In particular, we use a set of parameters to represent a particular class of MRs, which we refer to as polynomial MRs, and turn the problem of inferring MRs into a problem of searching for suitable values of the parameters. We then dynamically analyze multiple executions of the program, and use particle swarm optimization to solve the search problem. To improve the quality of inferred MRs, we further use MR filtering to remove some inferred MRs. We also conducted three empirical studies to evaluate our approach using four scientific libraries (including 189 scientific functions). From our empirical results, our approach is able to infer many high-quality MRs in acceptable time (i.e., from 9.87 seconds to 1231.16 seconds), which are effective in detecting faults with no false detection. ? 2014 ACM.EI

Obesity-related hypogonadism in women

Obesity-related hypogonadotropic hypogonadism is a well-characterized condition in men (termed male obesity-related secondary hypogonadism; MOSH); however, an equivalent condition has not been as clearly described in women. The prevalence of polycystic ovary syndrome (PCOS) is known to increase with obesity, but PCOS is more typically characterized by increased gonadotropin-releasing hormone (GnRH) (and by proxy luteinizing hormone; LH) pulsatility, rather than by the reduced gonadotropin levels observed in MOSH. Notably, LH levels and LH pulse amplitude are reduced with obesity, both in women with and without PCOS, suggesting that an obesity-related secondary hypogonadism may also exist in women akin to MOSH in men. Herein, we examine the evidence for the existence of a putative non-PCOS “female obesity-related secondary hypogonadism” (FOSH). We précis possible underlying mechanisms for the occurrence of hypogonadism in this context and consider how such mechanisms differ from MOSH in men, and from PCOS in women without obesity. In this review, we consider relevant etiological factors that are altered in obesity and that could impact on GnRH pulsatility to ascertain whether they could contribute to obesity-related secondary hypogonadism including: anti-Müllerian hormone, androgen, insulin, fatty acid, adiponectin, and leptin. More precise phenotyping of hypogonadism in women with obesity could provide further validation for non-PCOS FOSH and preface the ability to define/investigate such a condition

Real-Time Visualization and Quantitation of Vascular Permeability In Vivo: Implications for Drug Delivery

The leaky, heterogeneous vasculature of human tumors prevents the even distribution of systemic drugs within cancer tissues. However, techniques for studying vascular delivery systems in vivo often require complex mammalian models and time-consuming, surgical protocols. The developing chicken embryo is a well-established model for human cancer that is easily accessible for tumor imaging. To assess this model for the in vivo analysis of tumor permeability, human tumors were grown on the chorioallantoic membrane (CAM), a thin vascular membrane which overlays the growing chick embryo. The real-time movement of small fluorescent dextrans through the tumor vasculature and surrounding tissues were used to measure vascular leak within tumor xenografts. Dextran extravasation within tumor sites was selectively enhanced an interleukin-2 (IL-2) peptide fragment or vascular endothelial growth factor (VEGF). VEGF treatment increased vascular leak in the tumor core relative to surrounding normal tissue and increased doxorubicin uptake in human tumor xenografts. This new system easily visualizes vascular permeability changes in vivo and suggests that vascular permeability may be manipulated to improve chemotherapeutic targeting to tumors