Vaccine Development for Prescottella Equi

AbstractPrescottella equi (formerly Rhodococcus equi) is an intracellular pathogen that causes pyogranulomatous pneumonia in Thoroughbred foals. There is currently no vaccine available for the prevention of this disease in foals despite years of research. Cell mediated immunity is considered crucial for overcoming an infection caused by this pathogen. The virulence associated protein (VapA) is a well characterized immunogenic protein associated with this pathogen and was used to develop DNA and recombinant protein vaccines in this study. Vaccine candidates and live P. equi based vaccine were tested in BALB/c mice. Mice were challenged with virulent P.equi 2 weeks following the last boost and IgG subtypes and bacterial clearance from spleen and liver determined. The DNA vaccine elicited a significant IgG2a response indicating a Th1 biased immune response. The IFN gamma response from DNA and recombinant VapA vaccinated mice was moderate. The results of the challenge study showed that neither the recombinant VapA protein nor DNA vaccine enhanced clearance of P. equi in this model

The Play Behaviours of Roma Children in Transylvania

The Roma children of Transylvania are probably the most materially deprived in Europe. They often live in one-room shacks made from wood and mud, with no running water, no sanitation, and sometimes no heating. Many rely on charity for their food and medicines. But, are they play deprived? This paper summarises an observational study of the play behaviours of children in a small Roma village. It highlights the striking contrast between the abject poverty that characterises their lives and the general happiness of the children. These children live their limited lives to the full. They ‘play everywhere and with everything’, but not in the generally accepted sense of that phrase. The usual niceties of privacy, personal possessions and property boundaries are irrelevant here. Their play is rich in imagination and creativity; it is living proof of Nicholson’s theory of loose parts

Characterisation of the opposing effects of G6PD deficiency on cerebral malaria and severe malarial anaemia.

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is believed to confer protection against Plasmodium falciparum malaria, but the precise nature of the protective effecthas proved difficult to define as G6PD deficiency has multiple allelic variants with different effects in males and females, and it has heterogeneous effects on the clinical outcome of P. falciparum infection. Here we report an analysis of multiple allelic forms of G6PD deficiency in a large multi-centre case-control study of severe malaria, using the WHO classification of G6PD mutations to estimate each individual's level of enzyme activity from their genotype. Aggregated across all genotypes, we find that increasing levels of G6PD deficiency are associated with decreasing risk of cerebral malaria, but with increased risk of severe malarial anaemia. Models of balancing selection based on these findings indicate that an evolutionary trade-off between different clinical outcomes of P. falciparum infection could have been a major cause of the high levels of G6PD polymorphism seen in human populations

Health and economic imperatives for households in the context of the anti-Covid-19 strategy in Cameroon - the case of Yaounde

This paper explores the responses of households in the informal economic sector to the Cameroon government strategy against Covid-19 in Yaounde, Cameroon between March and May 2020. Given the recency of Covid-19, the exploratory design was employed to collect and analyse information for the study. Empirical data was obtained through personal observations and questionnaires, whereas grey data was sourced from official sources in government and international agencies in Yaounde. The mode of the ordinal data generated from the questionnaire was used to characterise the attitudes of respondents to quarantine measures and bar charts were used to illustrate the distribution of responses. The government's strategy against Covid-19 was largely ignored in Yaounde between March and May 2020 because of the influence of the predominantly informal economy on household's ability to allocate scarce resources between the competing needs of protecting their health on the one hand, and their livelihoods on the other hand. Poor households had to walk a difficult line between shutting down their businesses to protect their health or risking Covid-19 infections to protect their livelihoods. Over 53.1% of respondents thought quarantine measures were unsuccessful as over 63% ignored them. Quarantining and Social distancing were also difficult in informal settlements because of structural congestion. Perhaps, the greatest limitation of this study was the use of non-probability sampling. As such, sampling error could not be estimated, blurring the ability to ascertain the degree of similarity between the sample and the study population. This made sample generalisability difficult. There are short-term and long-term policy implications of these findings. Basic comprehensive measures including food and water distribution, as well as rents holidays must be implemented in informal neighbourhoods to ensure more successful quarantines in future pandemics. In the long-run, investments in urban social housing must be carried out to reduce slums, an ever-present risk factor in the rapid propagation of infections

Temperature Dependent Optical Band Gap Measurements of III-V films by Low Temperature Photoluminescence Spectroscopy

ABSTRACT Photoluminescence (PL) spectroscopy is a powerful technique for probing the structures of many types of III-V semiconductor materials. When a semiconductor material is excited at a particular wavelength, electron-hole pairs are generated. The most intense radiative transition is between the conduction band and valence band, and this measurement is used to determine the material band gap. Radiative and non-radiative transitions in semiconductors also involve localized defect levels. The photoluminescence energy associated with these levels can be used to identify specific defects, and the amount of photoluminescence can be used to determine their concentration, and thus predict device quality. At ambient temperatures, the PL signal is typically broad, as much as 100 nm in width. When cooled, structural details may be resolved, and a small spectral shift between 2 samples may represent a change in a structural parameter. Thus a system with high spectral resolution is required. In this paper, a modular Low Temperature Photoluminescence system (LTPL) for measuring optical band gap as a function of temperature is described. Results show that the optical band gap shifts towards higher energy as the sample temperature decreases