A Business Plan for The Fitzgerald Institute

This piece seeks to develop a business strategy for the University of Akron\u27s Fitzgerald Institute for Entrepreneurship. The recommendations given were developed utilizing research on entrepreneurial education and the desires of students at the university

Recent changes in the Labrador Sea Water within the Deep Western Boundary Current southeast of Cape Cod

Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Deep Sea Research Part I: Oceanographic Research Papers 58 (2011): 1019-1030, doi:10.1016/j.dsr.2011.07.006.Water properties measured by the central mooring in the Line W mooring array southeast of Cape Cod document a large character shift during the period of November 2001 to April 2008. The observed temperature, salinity and planetary potential vorticity (PPV) anomalies manifest changes in the formation region of the water masses present at Station W, specifically upper Labrador Sea Water (uLSW), deep Labrador Sea Water (dLSW) and Overflow Water (OW). During the observation period, the minimum in the PPV anomaly field relative to the record mean PPV profile migrated from 1500m, where it was originally found, to 700m. Temporal changes in the vertical distribution of temperature and salinity were correlated with the PPV changes. This suggests a dLSW-dominated first half of the record, versus an uLSW-dominated second half. The structure of these anomalies is consistent with observations within the Labrador Sea, and their transit time to Line W agrees well with tracer-derived times for signals spreading along the western boundary. In that context, the observed water properties at Line W in the early 2000s reflected the intense deep convection in the Labrador Sea in the mid 1990s, with less intense convection subsequently affecting lighter isopycnals. The observed velocity field is dominated by high-frequency (periods of days to months) fluctuations, however, a fraction of the velocity variability is correlated with changes in water mass properties, and indicate a gradual acceleration of the southwestward flow, with a corresponding increase in Deep Western Boundary Current transport.Financial support for the early observations (2001-2004) was provided by the G. Unger Vetlesen Foundation. Observations collected as part of the Line Wprogram (2004-2008) were funded by the U.S. National Science Foundation (grants number OCE-0241354 and OCE-0726720) as well as funding from WHOI’s Ocean and Climate Change Institute

Changes in the pool of Labrador Sea Water in the subpolar North Atlantic

Measurements of chlorofluorocarbon inventories during 1997–2003 allow the detection and quantification of significant changes in the formation rates of two modes of Labrador Sea Water (LSW): Upper (ULSW) and deep LSW, both here defined in fixed density intervals. Both modes contribute to the cold limb of the Meridional Overturning Circulation. Results reveal that the lighter ULSW formed since the mid-1990s has started to replace the large pool of the deep LSW stored in the western North Atlantic. Formation of deep LSW was absent in 1997–2003. Formation of ULSW compensated for this absence during 1998/99 (7.9 Sv), but afterwards significantly declined to 2.5 Sv. The decrease of the overall LSW formation throughout 1997–2003 correlates with a declining eastward baroclinic mass transport between the centers of the subpolar and subtropical gyres since 1997, a warming of LSW, and a gradually decreasing North Atlantic Oscillation index after 1999

How does Labrador Sea Water enter the deep western boundary current?

Author Posting. © American Meteorological Society, 2008. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 38 (2008): 968-983, doi:10.1175/2007JPO3807.1.Labrador Sea Water (LSW), a dense water mass formed by convection in the subpolar North Atlantic, is an important constituent of the meridional overturning circulation. Understanding how the water mass enters the deep western boundary current (DWBC), one of the primary pathways by which it exits the subpolar gyre, can shed light on the continuity between climate conditions in the formation region and their downstream signal. Using the trajectories of (profiling) autonomous Lagrangian circulation explorer [(P)ALACE] floats, operating between 1996 and 2002, three processes are evaluated for their role in the entry of Labrador Sea Water in the DWBC: 1) LSW is formed directly in the DWBC, 2) eddies flux LSW laterally from the interior Labrador Sea to the DWBC, and 3) a horizontally divergent mean flow advects LSW from the interior to the DWBC. A comparison of the heat flux associated with each of these three mechanisms suggests that all three contribute to the transformation of the boundary current as it transits the Labrador Sea. The formation of LSW directly in the DWBC and the eddy heat flux between the interior Labrador Sea and the DWBC may play leading roles in setting the interannual variability of the exported water mass.We are also grateful to the NSF for their support of this research

Interior pathways of the North Atlantic meridional overturning circulation

To understand how our global climate will change in response to natural and anthropogenic forcing, it is essential to determine how quickly and by what pathways climate change signals are transported throughout the global ocean, a vast reservoir for heat and carbon dioxide. Labrador Sea Water (LSW), formed by open ocean convection in the subpolar North Atlantic, is a particularly sensitive indicator of climate change on interannual to decadal timescales. Hydrographic observations made anywhere along the western boundary of the North Atlantic reveal a core of LSW at intermediate depths advected southward within the Deep Western Boundary Current (DWBC). These observations have led to the widely held view that the DWBC is the dominant pathway for the export of LSW from its formation site in the northern North Atlantic towards the Equator. Here we show that most of the recently ventilated LSW entering the subtropics follows interior, not DWBC, pathways. The interior pathways are revealed by trajectories of subsurface RAFOS floats released during the period 2003-2005 that recorded once-daily temperature, pressure and acoustically determined position for two years, and by model-simulated 'e-floats' released in the subpolar DWBC. The evidence points to a few specific locations around the Grand Banks where LSW is most often injected into the interior. These results have implications for deep ocean ventilation and suggest that the interior subtropical gyre should not be ignored when considering the Atlantic meridional overturning circulation.Dissertatio

Variability in the Deep Western Boundary Current : local versus remote forcing

Author Posting. © American Geophysical Union, 2012. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 117 (2012): C12022, doi:10.1029/2012JC008369.Horizontal velocity, temperature and salinity measurements from the Line W array for the period 2004–2008 show large changes in the water mass structure and circulation of the Deep Western Boundary Current (DWBC). Fluctuations in the flow with periods from 10 to 60 days are bottom intensified: signals most likely associated with topographic Rossby waves (TRW). A fraction (∼15%) of the DWBC transport variability is caused by Gulf Stream rings and meanders. These flow anomalies are surface intensified and fluctuate at frequencies lower than the TRW. Interannual variability in the velocity field appears to be related to changes in the hydrographic properties. The dominant mode of variability is characterized by an overall freshening, cooling, a potential vorticity (PV) increase in the deep Labrador Sea Water (dLSW) and a PV decrease in the Overflow Water (OW). The variability in the flow associated with these property changes is not spatially homogeneous. Offshore (water depths larger than 3500 m) changes in the velocity are in phase with PV changes in the OW: a decrease in the OW PV is accompanied by an increase in the southward (negative) transport. Conversely, variations of the inshore flow are in phase with changes in the dLSW PV (increasing PV and decreasing transport). This trend, true for most of the record, reverses after the winter of 2007–2008. A sudden decrease of the dLSW PV is observed, with a corresponding intensification of the flow in the inner DWBC as well as a northward shift in the Gulf Stream axis.Financial support for the Line W program (2004–2008) was provided by the U.S. National Science Foundation (grants OCE-0241354 and OCE-0726720) as well as funding from the WHOI’s Ocean and Climate Change Institute.2013-06-2

Changes in the CFC inventories and formation rates of upper Labrador Sea Water, 1997-2001

Chlorofluorocarbon (component CFC-11) and hydrographic data from 1997, 1999, and 2001 are presented to track the large-scale spreading of the Upper Labrador Sea Water (ULSW) in the subpolar gyre of the North Atlantic Ocean. ULSW is CFC rich and comparatively low in salinity. It is located on top of the denser “classical” Labrador Sea Water (LSW), defined in the density range σΘ = 27.68–27.74 kg m−3. It follows spreading pathways similar to LSW and has entered the eastern North Atlantic. Despite data gaps, the CFC-11 inventories of ULSW in the subpolar North Atlantic (40°–65°N) could be estimated within 11%. The inventory increased from 6.0 ± 0.6 million moles in 1997 to 8.1 ± 0.6 million moles in 1999 and to 9.5 ± 0.6 million moles in 2001. CFC-11 inventory estimates were used to determine ULSW formation rates for different periods. For 1970–97, the mean formation rate resulted in 3.2–3.3 Sv (Sv ≡ 106 m3 s−1). To obtain this estimate, 5.0 million moles of CFC-11 located in 1997 in the ULSW in the subtropical/tropical Atlantic were added to the inventory of the subpolar North Atlantic. An estimate of the mean combined ULSW/LSW formation rate for the same period gave 7.6–8.9 Sv. For the years 1998–99, the ULSW formation rate solely based on the subpolar North Atlantic CFC-11 inventories yielded 6.9–9.2 Sv. At this time, the lack of classical LSW formation was almost compensated for by the strongly pronounced ULSW formation. Indications are presented that the convection area needed in 1998–99 to form this amount of ULSW exceeded the available area in the Labrador Sea. The Irminger Sea might be considered as an additional region favoring ULSW formation. In 2000–01, ULSW formation weakened to 3.3–4.7 Sv. Time series of layer thickness based on historical data indicate that there exists considerable variability of ULSW and classical LSW formation on decadal scales

Clinician Knowledge and Beliefs after Statewide Program to Promote Appropriate Antimicrobial Drug Use

In 1999, Wisconsin initiated an educational campaign for primary care clinicians and the public to promote judicious antimicrobial drug use. We evaluated its impact on clinician knowledge and beliefs; Minnesota served as a control state. Results of pre- (1999) and post- (2002) campaign questionnaires indicated that Wisconsin clinicians perceived a significant decline in the proportion of patients requesting antimicrobial drugs (50% in 1999 to 30% in 2002; p<0.001) and in antimicrobial drug requests from parents for children (25% in 1999 to 20% in 2002; p = 0.004). Wisconsin clinicians were less influenced by nonpredictive clinical findings (purulent nasal discharge [p = 0.044], productive cough [p = 0.010]) in terms of antimicrobial drug prescribing. In 2002, clinicians from both states were less likely to recommend antimicrobial agent treatment for the adult case scenarios of viral respiratory illness. For the comparable pediatric case scenarios, only Wisconsin clinicians improved significantly from 1999 to 2002. Although clinicians in both states improved on several survey responses, greater overall improvement occurred in Wisconsin

Interrogating and Predicting Tolerated Sequence Diversity in Protein Folds: Application to E. elaterium Trypsin Inhibitor-II Cystine-Knot Miniprotein

Cystine-knot miniproteins (knottins) are promising molecular scaffolds for protein engineering applications. Members of the knottin family have multiple loops capable of displaying conformationally constrained polypeptides for molecular recognition. While previous studies have illustrated the potential of engineering knottins with modified loop sequences, a thorough exploration into the tolerated loop lengths and sequence space of a knottin scaffold has not been performed. In this work, we used the Ecballium elaterium trypsin inhibitor II (EETI) as a model member of the knottin family and constructed libraries of EETI loop-substituted variants with diversity in both amino acid sequence and loop length. Using yeast surface display, we isolated properly folded EETI loop-substituted clones and applied sequence analysis tools to assess the tolerated diversity of both amino acid sequence and loop length. In addition, we used covariance analysis to study the relationships between individual positions in the substituted loops, based on the expectation that correlated amino acid substitutions will occur between interacting residue pairs. We then used the results of our sequence and covariance analyses to successfully predict loop sequences that facilitated proper folding of the knottin when substituted into EETI loop 3. The sequence trends we observed in properly folded EETI loop-substituted clones will be useful for guiding future protein engineering efforts with this knottin scaffold. Furthermore, our findings demonstrate that the combination of directed evolution with sequence and covariance analyses can be a powerful tool for rational protein engineering