Sleep, health-related biological function and well-being

Sleep patterns are linked to cardiovascular outcomes and psychological well-being, but gaps in knowledge remain. This thesis tested four aspects of the relationships between sleep, cardiovascular risk and well-being using different methods of investigation; an analysis of a large population dataset (the English Longitudinal Study of Aging (Study 1), an investigation of affective and biological responses in everyday life of working women (Studies 2, 3), and a short-term well-being intervention (Study 4). Studies 1 and 2 tested whether direct biological dysregulation may be in part responsible for higher risk of cardiovascular outcomes in poor sleepers. Study 1 found that in older adults longer sleep was correlated with elevated inflammation, while short sleep was associated with low haemoglobin. Disturbed sleep was more prevalent among those with higher inflammation, lower dehydroepiandrosterone sulfate and haemoglobin as well as anaemia. These relationships were found mostly in men, but nonetheless they emphasise that self-reported sleep has important biological correlates in older adults. Study 2 extended data from experimental studies to real life settings, and found that disturbed sleep is related to lower heart rate variability (HRV). This suggests that lower HRV, a marker of dysfunctional autonomic activity, may be another pathway contributing towards higher risk of cardiovascular outcomes in poor sleepers. Study 3 compared objective and subjective measures of sleep efficiency and discovered that psychosocial characteristics including work stress and social support are related to underestimations of sleep efficiency, in comparison with objective measures. Thus associations between self-reported sleep and health-related factors may be overestimated in studies based on self-report. Study 4 aimed to induce positive well-being in a randomised controlled trial, to test whether this would lead to improvements in sleep. Well-being was increased post-intervention, but improvements in sleep were marginal. Importantly, changes in well-being were correlated with beneficial alternations in subjective sleep, tentatively suggesting that positive well-being may exert protective effects on (self-reported) sleep. In combination, these studies contribute to the research literature relating sleep problems with cardiovascular risk and poor psychological well-bein

The association between depressive and sleep symptoms for predicting incident disease onset after 6-year follow-up: findings from the English Longitudinal Study of Ageing

BACKGROUND: The independent effects of depressive symptoms and sleep problems for future physical illness risk have yet to be studied systematically across a variety of disease endpoints. METHODS: We analysed data from 7395 participants (65.81 ± 9.39 years; 54.8% female) from the English Longitudinal Study of Ageing (ELSA). Baseline was wave 4 and participants were followed up for 6 years until wave 7. Sleep was measured using an adapted version of the Jenkins Sleep Problems questionnaire and depressive symptoms using the Centre for Epidemiological Studies Depression scale. Participants with the illness of interest at baseline [coronary heart disease (CHD), cancer, diabetes/high blood glucose, arthritis] were excluded from models predicting the onset of that illness at follow-up. Logistic regression was used, entering depressive symptoms and sleep problems simultaneously into models controlling for a wide range of covariates. RESULTS: In fully adjusted models depressive symptoms predicted incident CHD (OR 1.11, 95% CI 1.04-1.20, p = 0.004) and diabetes/high blood glucose (OR 1.13, 95% CI 1.04-1.22, p = 0.002) independent of sleep problems; both depressive symptoms (OR 1.10, 95% CI 1.04-1.16, p = 0.002) and sleep problems (OR 1.14, 95% CI 1.02-1.26, p = 0.019) predicted incident arthritis. CONCLUSIONS: Sleep problems and depressive symptoms, and a combination of both, were differentially associated with physical illness onset 6 years later. Our findings highlight the importance of taking into account somatic and affective experiences when looking across a variety of different physical illnesses

Short sleep duration is associated with shorter telomere length in healthy men: findings from the Whitehall II cohort study.

Shorter telomere length and poor sleep are more prevalent at older ages, but their relationship is uncertain. This study explored associations between sleep duration and telomere length in a sample of healthy middle and early old age people

The impact of a brief gratitude intervention on subjective well-being, biology and sleep

This randomised controlled experiment tested whether a brief subjective well-being (SWB) intervention would have favourable effects on cardiovascular and neuroendocrine function and on sleep. We compared 2 weeks of a gratitude intervention with an active control (everyday events reporting) and no treatment conditions in 119 young women. The treatment elicited increases in hedonic well-being, optimism and sleep quality along with decreases in diastolic blood pressure. Improvements in SWB were correlated with increased sleep quality and reductions in blood pressure, but there were no relationships with cortisol. This brief intervention suggests that SWB may contribute towards lower morbidity and mortality through healthier biological function and restorative health behaviours

The role of intraoperative narrow-band imaging in transoral laser microsurgery for early and moderately advanced glottic cancer

Introduction: Trans-oral laser microsurgery is an established technique for the treatment of early and moderately advanced laryngeal cancer. Objective: The authors intend to test the usefulness of narrow-band imaging in the intraoperative assessment of the larynx mucosa in terms of specifying surgical margins. Methods: Forty-four consecutive T1-T2 glottic cancers treated with trans-oral laser microsurgery Type I-VI cordectomy were presented. Suspected areas (90 samples/44 patients) were biopsied under the guidance of narrow-band imaging and white light and sent for frozen section. Results: Our study revealed that 75 of 90 (83.3%) white light and narrow-band imaging-guided samples were histopathologically positive: 30 (40%) were confirmed as carcinoma in situ or invasive carcinoma and 45 (60%) as moderate to severe dysplasia. In 6 patients mucosa was suspected only in narrow-band imaging, with no suspicion under white light. Thus, in these 6 patients 18/90 (20%) samples were taken. In 5/6 patients 16/18 (88.8%) samples were positive in frozen section: in 6/18 (33.3%) carcinoma (2 patients), 10/18 (66.6%) severe dysplasia was confirmed (3 patients). In 1 patient 2/18 (11.1%) samples were negative in frozen section. Presented analysis showed, that sensitivity, specificity and accuracy of white light was 79.5%, 20% and 71.1% respectively, while narrow-band imaging was 100%, 0.0% and 85.7%, respectively. Conclusion: The intraoperative use of narrow-band imaging proved to be valuable in the visualization of suspect areas of the mucosa. Narrow-band imaging confirms the suspicions undertaken in white light and importantly, it showed microlesions beyond the scope of white light

The chemical and electrochemical oxidative polymerization of 2-amino-4-tert-butylphenol

[EN] Poly(2-amino-4-tert-butylphenol), poly(2A-4TBP), was synthesized from monomer aqueous solution using either electrochemical or chemical oxidation procedures. Several spectroscopic characterization techniques were employed to gain information on the chemical structure and redox behavior of the obtained materials. It was found that the chemical polymerization product could be described as an oligomer mixture containing up to 16 monomer units. In parallel to other polymers derived from o-aminophenol, phenoxazine rings constitute also the basic structure of poly(2A-4TBP). In addition, the occurrence of N-N couplings, which are favored by the presence of the voluminous tert-butyl substituent, seems also relevant. No significant structural differences were found between the chemically or electrochemically synthesized materials. © 2016 Published by Elsevier Ltd.Financial support from the Spanish Ministerio de Economía y Competitividad and FEDER funds (MAT2013-42007-P) and from the Generalitat Valenciana (PROMETEO2013/038) is gratefully acknowledged. M. Abidi thanks the Ministry of Higher Education and Scientific Research of Tunisia for funding her stay at the University of Alicante.Abidi, M.; López-Bernabeu, S.; Huerta, F.; Montilla-Jiménez, F.; Besbes-Hentati, S.; Morallón, E. (2016). The chemical and electrochemical oxidative polymerization of 2-amino-4-tert-butylphenol. Electrochimica Acta. 212:958-965. https://doi.org/10.1016/j.electacta.2016.07.060S95896521

Effectiveness of cidofovir intralesional treatment in recurrent respiratory papillomatosis

To present the results of recurrent respiratory papillomatosis (RRP) treatment with surgical excision and adjuvant anti-viral cidofovir intralesional use and to examine the correlation between the cidofovir effectiveness and the patient previous history of multiple larynx procedures, age, extension of lesion and dose. 32 patients with laryngeal papillomas were treated with cidofovir in our Department between I.2009 and I.2011. The number of previous RRP debulking procedures ranged from 1 to 100. The intensity of papillomatosis differed from one anatomic site and moderate growth to four or five localizations with heavy extension. The number of injections per patient varied from 1 to 7, and the total volume of 5 mg/ml solution varied from 2 to 33 ml. The injections were combined with laser debulking of the lesions. In disperse papillomata, the injections were administered in particular anatomical sites in 4–6 weeks intervals, in massive lesions injections were repeated in the same anatomical site in 2–4 weeks. Complete remission was observed in 18 out of 32 patients. 13 patients showed remission in a place of cidofovir injection. One patient did not react to the drug. In four patients, new changes in injection places appeared. In two patients, hepatic toxic side effects were observed. Intralesional cidofovir injection has been shown to be an effective and safe therapy for laryngeal papillomatosis and should be considered in those patients who experienced disease relapse

Identification, frequency, activation and function of CD4+ CD25highFoxP3+ regulatory T cells in children with juvenile idiopathic arthritis

The aim of the study was to test the frequency of CD4+ CD25highFoxP3 regulatory T cells in JIA patients and to assess their activation status and functional activity. The study involved 12 children with JIA and 35 healthy control subjects. PBMC were stained with monoclonal antibodies (anti-CD25, anti-CD4, anti-CD127, anti-CD69, anti-CD71, and anti-FoxP3). The samples were evaluated using flow cytometer. CD4+ CD25− and CD4+ CD25+ cells were isolated by negative and positive selection with magnetic microbeads. CD4+ CD25+ and CD4+ CD25− cells were cultured separately and co-cultured (1:1) with or without PHA. The percentage of Tregs in JIA patients was significantly decreased in comparison with controls (median, 3.2 vs. 4.6; P = 0.042). Relative fluorescence intensities of FoxP3 were higher in JIA patients than in controls (median, 9.1 vs. 6.8). The percentage of activated Tregs (CD71+) was significantly higher in JIA patients in comparison with controls (median, 6.5 vs. 2.8; P = 0.00043). CD4+ CD25+ cells derived from JIA patients and controls were anergic upon PHA stimulation, while CD4+ CD25− cells showed intensive proliferative response. The proliferation rate of CD4+ CD25− cells stimulated by PHA was decreased in co-cultures. In JIA patients, the inhibition of proliferation of CD4+ CD25− cells by CD4+ CD25+ cells was 37.9%, whereas in controls it was significantly lower (55.7%, P = 0.046). JIA patients had statistically lower percentage of Tregs in peripheral blood compared to controls. CD4+ CD25+ cells sorted from peripheral blood of JIA patients had statistically lower ability to suppress CD4+ CD25− cell proliferation in comparison with cells obtained from controls