Source identification and quantification of particulate matter emitted from livestock houses

There is need to identify and quantify the contribution of different sources to airborne particulate matter (PM) emissions from animal houses. To this end, we compared the chemical and morphological characteristics of fine and coarse PM from known sources collected from animal houses with the characteristics of on-farm fine and coarse airborne PM using two methods: classification rules based on decision trees and multiple linear regression. Fourteen different farms corresponding to seven different housing systems for poultry and pigs were sampled during winter. A total of 28 fine and 28 coarse on-farm airborne PM samples were collected, together with a representative sample of each known source per farm (56 known source samples in total). Source contributions were calculated as relative percentage contributions in particle numbers and then estimated in particle mass. Based on particle numbers, results showed that in poultry houses, most on-farm airborne PM originated from feathers (ranging from 4% to 43% in fine PM and from 6% to 35% in coarse PM) and manure (ranging from 9% to 85% in fine PM and from 30% to 94% in coarse PM). For pigs, most on-farm airborne PM originated from manure (ranging from 70% to 98% in fine PM and from 41% to 94% in coarse PM). Based on particle mass, for poultry most on-farm airborne PM still originated from feathers and manure; for pigs, however, most PM originated from skin and manure. Feed had a negligible contribution to on-farm airborne PM compared with other sources. Results presented in this study improve the understanding of sources of PM in different animal housing systems, which may be valuable when choosing optimal PM reduction technique

Besnoitia besnoiti infection alters both endogenous cholesterol de novo synthesis and exogenous LDL uptake in host endothelial cells

Besnoitia besnoiti, an apicomplexan parasite of cattle being considered as emergent in Europe, replicates fast in host endothelial cells during acute infection and is in considerable need for energy, lipids and other building blocks for offspring formation. Apicomplexa are generally considered as defective in cholesterol synthesis and have to scavenge cholesterol from their host cells for successful replication. Therefore, we here analysed the influence of B. besnoiti on host cellular endogenous cholesterol synthesis and on sterol uptake from exogenous sources. GC-MS-based profiling of cholesterol-related sterols revealed enhanced cholesterol synthesis rates in B. besnoiti-infected cells. Accordingly, lovastatin and zaragozic acid treatments diminished tachyzoite production. Moreover, increased lipid droplet contents and enhanced cholesterol esterification was detected and inhibition of the latter significantly blocked parasite proliferation. Furthermore, artificial increase of host cellular lipid droplet disposability boosted parasite proliferation. Interestingly, lectin-like oxidized low density lipoprotein receptor 1 expression was upregulated in infected endothelial hostcells, whilst low density lipoproteins (LDL) receptor was not affected by parasite infection. However, exogenous supplementations with non-modified and acetylated LDL both boosted B. besnoiti proliferation. Overall, current data show that B. besnoiti simultaneously exploits both, endogenous cholesterol biosynthesis and cholesterol uptake from exogenous sources, during asexual replication

Monitoring of the Canales Dam and Its Control During Construction Period

This paper presents a study of the stress-deformation behaviour of the Canales Dam (Granada) during construction. The basis for this study has been the three dimensional (3D) finite elements method with hyperbolic material response. The analytical procedure used is presented and the study concludes with a comparative study of the results obtained from the calculation programme used and monitoring system\u27s measurements

An easy synthetic way to exfoliate and stabilize MWCNTs in a thermoplastic pyrrole-containing matrix assisted by hydrogen bonds

This work focuses on the design of an engineered thermoplastic polymer containing pyrrole units in the main chain and hydroxyl pendant groups (A-PPy-OH), which help in achieving nanocomposites containing well-distributed, exfoliated and undamaged MWCNTs. The thermal annealing at 100 °C of the pristine nanocomposite promotes the redistribution of the nanotubes in terms of a percolative network, thus converting the insulating material in a conducting soft matrix (60 μΩ m). This network remains unaltered after cooling to r.t. and successive heating cycles up to 100 °C thanks to the effective stabilization of MWCNTs provided by the functional polymer matrix. Notably, the resistivity-temperature profile is very reproducible and with a negative temperature coefficient of -0.002 K-1, which suggests the potential application of the composite as a temperature sensor. Overall, the industrial scale by which A-PPy-OH can be produced offers a straightforward alternative for the scale-up production of suitable polymers to generate multifunctional nanocomposites

Endoparasitic insights of free-living fin (Balaenoptera physalus), Humpback (Megaptera novaeangliae) and North Atlantic Right Whales (Eubalaena glacialis) from Eastern Canadian Waters

Open Access funding enabled and organized by Projekt DEAL.Purpose: To date, little is still known on parasite infections affecting free-living large whale populations worldwide. Data presented should be considered as a baseline study for future monitoring surveys on endoparasites affecting whales, thereby enhancing investigations on impacts of zoonotic parasitoses not only on vulnerable or endangered baleen whale population health but also on public health. Methods: The presented study is a first report on gastrointestinal parasites infecting different free-living baleen whales inhabiting East Canadian waters using non-invasive methods. Individual faecal samples from fin (n = 3; Balaenoptera physalus), humpback (n = 4; Megaptera novaeangliae) and North Atlantic right whales (n = 1; Eubalaena glacialis) were collected without animal disturbance, within their natural habitats on an ecological expedition during annual surveys in summer 2017. Faecal samples were assessed by standardized diagnostic methods, such as sodium acetate acetic formalin (SAF) technique, carbol fuchsin-stained faecal smears, Giardia/Cryptosporidium coproantigen ELISAs and were applied for further identification. Results: Parasitological infections included three different potentially zoonotic parasite species, one protozoa (Entamoeba spp.) and two metazoans (Diphyllobothriidae gen. sp., Ascaridida indet.). No positive Giardia/Cryptosporidium coproantigen ELISA could be found in the studied whales. Conclusion: This study adds to the current knowledge of intestinal and zoonotic parasite infections of vulnerable to partly endangered free-ranging baleen whales. Only few or no parasitological studies exist for these whale species, usually dealing with only one dead specimen. We call for more research in this field especially for the importance of conservation of free-living marine mammals using non-invasive methods.Publisher PDFPeer reviewe

Cross-linking of rubber in the presence of multi-functional cross-linking aids via thermoreversible Diels-Alder chemistry

Furan-functionalized polyketone (PK-FU) was added to a furan-functionalized ethylene-propylene rubber (EPM-FU). The mixture was subsequently cross-linked with a bismaleimide through Diels-Alder chemistry in order to improve the mechanical properties of the rubber. Infrared spectroscopy showed the reversible interaction between both polymers and the bismaleimide cross-linker. Likewise, mechanical measurements indicated the re-workability of the mixtures with no evident differences in storage modulus and mechanical properties after several heating cycles. The cross-link density and mechanical properties, such as hardness, tensile properties and compression set, could be modulated by changing the degree of furan functionalization of PK-FU and the PK-FU loading in the blends. It is concluded that PK-FU has some characteristics of an inert filler, but mainly acts as a multi-functional cross-linking aid, enabling larger amounts of bismaleimide to cross-link EPM-FU

The Rap Net: a geodetic positioning network for Andalusia (South Spain)

In this paper we present a description of the Andalusian Positioning Network, RAP: its objectives; design; development; its problems and its present status. This geodetic network consists of 22 permanent GPS stations whose surveying will provide the data required to obtain relative positions of any place in Andalusia after applying some post-processing techniques and real-time differential corrections. Thus, high-precision geodetic coordinates referred to the WGS-84 system will be provided for anywhere in Andalusia. The station in the network can broadcast a differential correction via internet. Nine stations can also broadcast the RTK corrections via radio. The network has been designed to cover Andalusia and to provide real-time differential corrections in the whole area. The RAP network is referred to the EUREF system and is the new reference frame in Andalusia for the Institute of Cartography of Andalusia’s new cartography. It will also be used to settle photogrametric points or marks for the georeferencing of satellite images; to establish control points for reference networks in civil engineering or GIS applications; for numerous scientific and technological applications, such as precise geoid determination and tropospheric, ionospheric, and climatological studies, among others. Its millimetric precision will ensure success when integrating new projects with other cartographies

Periodization and programming for individual 400 m medley swimmers

Knowledge in the scientific domain of individual medley (IM) swimming training over a competitive season is limited. The purpose of this study was to propose a detailed coaching framework incorporating the key elements of a periodized training regimen for a 400 m IM swimmer. This framework was based on the available coaching and scientific literature and the practical experience and expertise of the collaborating authors. The season has been divided in two or three macrocycles, further divided in three mesocycles each (six or nine mesocycles in total), in alignment with the two or three main competitions in each macrocycle. The principal training contents to develop during the season expressed in blood lactate zones are: aerobic training (~2 mmol·L(−1)), lactate threshold pace (~4 mmol·L(−1)) and VO(2)max (maximum oxygen uptake) (~6 mmol·L(−1)). Strength training should focus on maximum strength, power and speed endurance during the season. Altitude training camps can be placed strategically within the training season to promote physiological adaptation and improvements in performance. A well-constructed technical framework will permit development of training strategies for the 400 m IM swimmer to improve both training and competitive performance

Effects of Dry-Land Training Programs on Swimming Turn Performance: A Systematic Review.

Swimming coaches have prescribed dry-land training programs over the years to improve the overall swimming performance (starts, clean swimming, turns and finish). The main aim of the present systematic review was to examine the effects of dry-land strength and conditioning programs on swimming turns. Four online databases were scrutinised, data were extracted using the Preferred PRISMA guidelines and the PEDro scale was applied. A total of 1259 articles were retrieved from database searches. From the 19 studies which were full-text evaluated, six studies were included in the review process. The review indicated that plyometric, strength, ballistic and core training programs were implemented for improving swimming turn performance. Strength, ballistic and plyometric training focusing on neural enhancement seem to be effective for improving swimming turn performance. The data related to training of the core were not conclusive. Coaches should consider incorporating exercises focusing on improving the neuromuscular factor of the leg-extensor muscles into their daily dry-land training programs. More researches are needed to provide a better understanding of the training methods effects and training organisations for improving swimming turn performance.post-print626 K

Synthesis of poly(1-vinylimidazole)-block-poly(9-vinylcarbazole) copolymers via RAFT and their use in chemically responsive graphitic composites

This study reports the synthesis of novel poly(1-vinylimidazole)-b-poly(9-vinylcarbazole) (PVI-b-PVK) block copolymers with varying monomer ratios using reversible addition-fragmentation chain-transfer (RAFT) polymerization and their incorporation in responsive composite materials. Specifically, non-covalent exfoliation of two different conductive fillers, multi-walled carbon nanotubes (MWCNTs) or reduced graphene oxide (rGO), was studied. The percolation threshold of the synthesized nanocomposites was dependent on the polymer used for dispersion, showing a better affinity of the fillers for block copolymers with higher relative carbazole content. Resistivity measurements showed selective variation in the resistance signal when the materials were exposed to various organic solvents and acids, providing a good basis for the design of sensing devices