Horticultural uses of botanical variations of woodland sage (Salvia nemorosa L.)

On the basis of the facts it may state that the Salvia nemorosa is an „underestimated” plant at present, but it may be a promising plant species for the future. This is especially true in the Central and Eastern Europe, a region exposed to certain climate changes, such as the more extreme weather, the fluctuation of rainfall distribution, the increasing of temperature, the rapid and unfavourable changes of climate in densely populated cities, the heat-island effects, the atmospheric drought, etc. Therefore, the Salvia nemorosa seems to be a very promising plant for the future generations. The searching and collecting program of the wild botanical variants of woodland sage were launched in 2009, as part of two botanical expeditions carried out in Hungary

Production of dry type artificial seeds by means of PVC-FOIL desiccation method from genetically engineered somatic embryos of carrot (Daucus carota L., cv. Nantes Duke).

Under the experimental condition used, the pattern of desiccation was linear. The speed of water loss have been very slow, 4 mg/h and after 48 hours, an average 15% water content was measured

Influence of antiobiotics on NAA- induced somatic embryogenesis in eggplant (Solanum melongena L. cv. Embu).

The influence of increasing concentrations of naphthaleneacetic acid and the antibiotics cefotaxim?, timentin, kanamycin, and hygromycin on eggplant (Solanum melongena L. cv. Emb£) somatic embryogenesis was investigated. Cotyledon explants were excised from 16 to 20 days old in vitro grown seedlings. NAA promoted somatic embryogenesis, although its concentrations had no influence on the mean number of embryos. Callusing decreased significantly with increasing NAA concenti-ations. Morphogenesis was stopped with 50 to 100 mg L-I kanamycin and 7.5 to 15 mg L-I hygromycin. Although early globular embryos were observed up to 15 mg L-I, further embryo development was inhibited at 10 mg L .1. Interestingly, cefotaxime (250 and 500 mg L -1) promoted a marked effect on enhancing fresh weight of calli, accompanied by decrease in embryo regeneration, whereas timentin concentrations (150 and 300 mg L-I) did not affect embryo differentiation as compared to the control treatment

Regeneração de tomate industrial a partir do cultivo "in vitro" de cotilédones "em fenda" visando transformação genética.

As plântulas enraizadas foram transferidas para subtrato esterilizado

Regeneracao in vitro de tomate industrial visando a transformacao genetica.

Investigacoes foram iniciadas no Laboratorio de Biotecnologia da Embrapa-CPATSA visando estabelecer um protocolo para transformacao genetica de algumas variedades nacionais importantes de tomate industrial. Inicialmente, as variedades IPA-5 e IPA-6 foram cultivadas utilizando-se quatro formulas do meio de cultura de tecidos e cinco variacoes de inoculacoes. As gemas com primordios foliares obtidos foram subcultivadas em meio MS (1962) suplementado com 0,5 mg/l de zeatina. Logo apos, os brotos mais desenvolvidos foram transferidos para meio basal modificado de MS, regenerando plantas completas com brotos e raizes que, em seguida, foram aclimatadas em casa-de-vegetacao. Na variedade IPA-5, as frequencias mais altas de inducao de regeneracao variaram entre 76% e 100%. No caso da IPA-6 obteve-se frequencias entre 52,6% e 75%. Com base nesta metodologia, estabeleceu-se a tecnica para transformacao genetica via vetor Agrobacterium tumefaciens pGS-gluc1, atraves dos genes reporter GUS e o marcador de selecao NPT-II, obtendo-se tambem resultados significativos com genes que conferem resistencia contra insetos

BASH: a tool for managing BeadArray spatial artefacts

Summary: With their many replicates and their random layouts, Illumina BeadArrays provide greater scope fordetecting spatial artefacts than do other microarray technologies. They are also robust to artefact exclusion, yet there is a lack of tools that can perform these tasks for Illumina. We present BASH, a tool for this purpose. BASH adopts the concepts of Harshlight, but implements them in a manner that utilizes the unique characteristics of the Illumina technology. Using bead-level data, spatial artefacts of various kinds can thus be identified and excluded from further analyses

Germinação in vitro de orquídea.

Esta pesquisa tem como objetivo a germinação "in vitro" de orquídea (Cattleya dowiana aurea) visando otimizar protocolo de micropropagação para a espécie

Prevalence and distribution of vascular calcifications at CT scan in patients with and without large vessel vasculitis: A matched cross-sectional study

Objectives The aim of this study was to compare the prevalence, entity and local distribution of arterial wall calcifications evaluated on CT scans in patients with large vessel vasculitis (LVV) and patients with lymphoma as reference for the population without LVV. Methods All consecutive patients diagnosed with LVVs with available baseline positron emission tomography-CT (PET-CT) scan performed between 2007 and 2019 were included; non-LVV patients were lymphoma patients matched by age (±5 years), sex and year of baseline PET-CT (≤2013; >2013). CT images derived from baseline PET-CT scans of both patient groups were retrospectively reviewed by a single radiologist who, after setting a threshold of minimum 130 Hounsfield units, semiautomatically computed vascular calcifications in three separate locations (coronaries, thoracic and abdominal arteries), quantified as Agatston and volume scores. Results A total of 266 patients were included. Abdominal artery calcifications were equally distributed (mean volume 3220 in LVVs and 2712 in lymphomas). Being in the LVVs group was associated with the presence of thoracic calcifications after adjusting by age and year of diagnosis (OR 4.13, 95% CI 1.35 to 12.66; p=0.013). Similarly, LVVs group was significantly associated with the volume score in the thoracic arteries (p=0.048). In patients >50 years old, calcifications in the coronaries were more extended in non-LVV patients (p=0.027 for volume). Conclusion When compared with patients without LVVs, LVVs patients have higher calcifications in the thoracic arteries, but not in coronary and abdominal arteries

Concurrent declines in malaria incidence among children under and over five years of age in Koutiala, Mali: time series analysis of seasonal malaria chemoprevention from 2012-2022

In 2012, the World Health Organization recommended seasonal malaria chemoprevention (SMC) for children 3–59 months old in areas of highly seasonal malaria transmission. Long-term impact of SMC on malaria incidence is unknown. In Koutiala health district, a random sample of 10 villages were selected surrounding health facilities with 1:3 urban/rural ratio. Cases of uncomplicated and complicated malaria, confirmed by rapid diagnostic test or microscopy, were documented monthly at facilities prior to SMC (2011) and each year of SMC (2012-2022). We used Poisson regression with robust standard errors adjusting for repeated measurements within villages to estimate rates in 2011 and change over time (with interaction terms to compare differences in rates over time across groups as applicable), assuming approximately linear trends of annual rates over time. Overall, 1429 village-level observations were included. In 2011, estimated confirmed uncomplicated malaria was 60 cases (95%CI 16, 218) and 8 cases (95%CI 4, 14)/1000 population among < 5-year olds and ≥ 5-year olds, respectively. From 2012 to 2022, the confirmed uncomplicated incidence among < 5 year-olds declined by 8 (95%CI -2, 18) cases/1000 pop, and among ≥ 5-year olds by 3 (95%CI -4, 8) cases/1000 pop annually, a difference of 5 cases (95%CI 0,11)/1000 pop (p=0.060). In the general population, confirmed uncomplicated and confirmed complicated malaria declined by 6 cases (95%CI -3, 15, p=0.203) and 4 cases (95%CI -1, 10, p=0.128)/1000 pop annually, respectively. There was little to no evidence of declining rates in rural and urban areas (interaction p=0.083 and p=0.589 for cum and ccm, respectively). There was no evidence of difference in rates of confirmed uncomplicated and confirmed complicated cases between 2011-2022 overall (interaction p=0.617), among < 5-year olds (interaction p=0.732), nor ≥ 5-year olds (interaction p=0.850). SMC was associated with reduced incidence of confirmed uncomplicated and complicated cases among children < 5-year olds over 10-year of SMC in Koutiala, Mali. Molecular surveillance is urgently needed to confirm this apparent trend

Two-Year Pulmonary Safety and Efficacy of Inhaled Human Insulin (Exubera) in Adult Patients With Type 2 Diabetes

OBJECTIVE—The purpose of this study was to evaluate the 2-year pulmonary safety of inhaled human insulin (Exubera [EXU]) in 635 nonsmoking adults with type 2 diabetes