42 research outputs found

    Numerical study of the effects of boundary conditions on the measurement and calibration of gardon type heat flux sensors

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    To monitor the high-intensity heat flux conditions that occur in the space shuttle main engine (SSME), it is necessary to use specifically designed heat flux sensors. These sensors, which are of the Gardon-type, are exposed on the measuring face to high-intensity radiative and convective heat fluxes and on the other face to convective cooling. To improve the calibration and measurement accuracy of these gauges, researchers are studing the effect that the thermal boundary conditions have on gauge performance. In particular, they are studying how convective cooling effects the field inside the sensor and the measured heat flux. The first phase of this study involves a numerical study of these effects. Subsequent phases will involve experimental verification. A computer model of the heat transfer around a Garden-type heat flux sensor was developed. Two specific geometries are being considered are: (1) heat flux sensor mounted on a flat-plate; and (2) heat flux sensor mounted at the stagnation point of a circular cylinder. Both of these configurations are representative of the use of heat flux sensors in the components of the SSME. The purpose of the analysis is to obtain a temperature distribution as a function of the boundary conditions

    Using Microarrays to Facilitate Positional Cloning: Identification of Tomosyn as an Inhibitor of Neurosecretion

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    Forward genetic screens have been used as a powerful strategy to dissect complex biological pathways in many model systems. A significant limitation of this approach has been the time-consuming and costly process of positional cloning and molecular characterization of the mutations isolated in these screens. Here, the authors describe a strategy using microarray hybridizations to facilitate positional cloning. This method relies on the fact that premature stop codons (i.e., nonsense mutations) constitute a frequent class of mutations isolated in screens and that nonsense mutant messenger RNAs are efficiently degraded by the conserved nonsense-mediated decay pathway. They validate this strategy by identifying two previously uncharacterized mutations: (1) tom-1, a mutation found in a forward genetic screen for enhanced acetylcholine secretion in Caenorhabditis elegans, and (2) an apparently spontaneous mutation in the hif-1 transcription factor gene. They further demonstrate the broad applicability of this strategy using other known mutants in C. elegans, Arabidopsis, and mouse. Characterization of tom-1 mutants suggests that TOM-1, the C. elegans ortholog of mammalian tomosyn, functions as an endogenous inhibitor of neurotransmitter secretion. These results also suggest that microarray hybridizations have the potential to significantly reduce the time and effort required for positional cloning

    Computational Fluid Dynamics of Catalytic Reactors

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    Today, the challenge in chemical and material synthesis is not only the development of new catalysts and supports to synthesize a desired product, but also the understanding of the interaction of the catalyst with the surrounding flow field. Computational Fluid Dynamics or CFD is the analysis of fluid flow, heat and mass transfer and chemical reactions by means of computer-based numerical simulations. CFD has matured into a powerful tool with a wide range of applications in industry and academia. From a reaction engineering perspective, main advantages are reduction of time and costs for reactor design and optimization, and the ability to study systems where experiments can hardly be performed, e.g., hazardous conditions or beyond normal operation limits. However, the simulation results will always remain a reflection of the uncertainty in the underlying models and physicochemical parameters so that in general a careful experimental validation is required. This chapter introduces the application of CFD simulations in heterogeneous catalysis. Catalytic reactors can be classified by the geometrical design of the catalyst material (e.g. monoliths, particles, pellets, washcoats). Approaches for modeling and numerical simulation of the various catalyst types are presented. Focus is put on the principal concepts for coupling the physical and chemical processes on different levels of details, and on illustrative applications. Models for surface reaction kinetics and turbulence are described and an overview on available numerical methods and computational tools is provided

    Profiling Synaptic Proteins Identifies Regulators of Insulin Secretion and Lifespan

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    Cells are organized into distinct compartments to perform specific tasks with spatial precision. In neurons, presynaptic specializations are biochemically complex subcellular structures dedicated to neurotransmitter secretion. Activity-dependent changes in the abundance of presynaptic proteins are thought to endow synapses with different functional states; however, relatively little is known about the rules that govern changes in the composition of presynaptic terminals. We describe a genetic strategy to systematically analyze protein localization at Caenorhabditis elegans presynaptic specializations. Nine presynaptic proteins were GFP-tagged, allowing visualization of multiple presynaptic structures. Changes in the distribution and abundance of these proteins were quantified in 25 mutants that alter different aspects of neurotransmission. Global analysis of these data identified novel relationships between particular presynaptic components and provides a new method to compare gene functions by identifying shared protein localization phenotypes. Using this strategy, we identified several genes that regulate secretion of insulin-like growth factors (IGFs) and influence lifespan in a manner dependent on insulin/IGF signaling

    Defects in tRNA Modification Associated with Neurological and Developmental Dysfunctions in Caenorhabditis elegans Elongator Mutants

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    Elongator is a six subunit protein complex, conserved from yeast to humans. Mutations in the human Elongator homologue, hELP1, are associated with the neurological disease familial dysautonomia. However, how Elongator functions in metazoans, and how the human mutations affect neural functions is incompletely understood. Here we show that in Caenorhabditis elegans, ELPC-1 and ELPC-3, components of the Elongator complex, are required for the formation of the 5-carbamoylmethyl and 5-methylcarboxymethyl side chains of wobble uridines in tRNA. The lack of these modifications leads to defects in translation in C. elegans. ELPC-1::GFP and ELPC-3::GFP reporters are strongly expressed in a subset of chemosensory neurons required for salt chemotaxis learning. elpc-1 or elpc-3 gene inactivation causes a defect in this process, associated with a posttranscriptional reduction of neuropeptide and a decreased accumulation of acetylcholine in the synaptic cleft. elpc-1 and elpc-3 mutations are synthetic lethal together with those in tuc-1, which is required for thiolation of tRNAs having the 5′methylcarboxymethyl side chain. elpc-1; tuc-1 and elpc-3; tuc-1 double mutants display developmental defects. Our results suggest that, by its effect on tRNA modification, Elongator promotes both neural function and development

    Analysis of mRNA Abundance in <i>mec-3(e1338)</i> Animals

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    <p>Fold-change (<i>x</i>-axis) is plotted against the statistical significance (<i>y</i>-axis) for each probeset. Fold-changes are shown on log<sub>2</sub> scale. <i>p-</i>Values are shown on a negative log<sub>10</sub> scale. The symbol × indicates genes with reduced expression in <i>mec-3(e1338)</i> animals (fold-change < −1, <i>p</i> < 0.01). Light blue circles indicate genes with reduced expression that are also on Chromosome 4. Dark blue circles indicate genes with reduced expression that are within 1 cM to the left or right of <i>mec-3</i>. The open red circle indicates the <i>mec-3</i> gene.</p

    Analysis of mRNA Abundance in the KP3365 <i>unc-43(n1186)</i> CaMKII Strain

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    <p>Expression data are illustrated as described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0010002#pgen-0010002-g002" target="_blank">Figure 2</a>. The symbol × indicates probesets with reduced expression in KP3365 animals (fold-change < −0.5, <i>p</i> < 0.01). Filled blue circles indicate probesets with reduced expression that are also on Chromosome 4. Open red circles indicate probesets corresponding to the <i>unc-43</i> CaMKII gene. The black square indicates the probeset corresponding to the <i>hif-1</i> gene<i>.</i></p
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