Dynamic studies of antibody-antigen interactions with an electrolyte-gated organic transistor

Affinity-based biosensors employing surface-bound biomolecules for analyte detection are important tools in clinical diagnostics and drug development. In this context, electrolyte-gated organic transistors (EGOTs) are emerging as ultrasensitive label-free biosensors. In this study, we present an EGOT sensor integrated within a microfluidic system. The sensor utilizes the cytomegalovirus (CMV) phosphoprotein 65 as a biorecognition element to detect the pathological biomarker human anti-cytomegalovirus antibody in solution. The biorecognition element is grafted onto the gate electrode by exploiting the polyhistidine-tag technology. Real-time monitoring of the EGOT response, coupled with a twocompartment kinetic model analysis, enables the determination of analyte concentration, binding kinetics, and thermodynamics of the interaction. The analysis of the relevant kinetic parameters of the binding process yields a reliable value for the thermodynamic equilibrium constant and suggests that the measured deviations from the Langmuir binding model arise from the co-existence of binding sites with different affinities toward the antibodies

The impact of plasmonic electrodes on the photocarrier extraction of inverted organic bulk heterojunction solar cells

Nano patterning the semiconducting photoactive layer back electrode interface of organic photovoltaic devices is a widely accepted approach to enhance the power conversion efficiency through the exploitation of numerous photonic and plasmonic effects. Yet, nano patterning the semiconductor metal interface leads to intertwined effects that impact the optical as well as the electrical characteristic of solar cells. In this work we aim to disentangle the optical and electrical effects of a nano structured semiconductor metal interface on the device performance. For this, we use an inverted bulk heterojunction P3HT PCBM solar cell structure, where the nano patterned photoactive layer back electrode interface is realized by patterning the active layer with sinusoidal grating profiles bearing a periodicity of 300 nm or 400 nm through imprint lithography while varying the photoactive layer thickness LPAL between 90 and 400 nm. The optical and electrical device characteristics of nano patterned solar cells are compared to the characteristics of control devices, featuring a planar photoactive layer back electrode interface. We find that patterned solar cells show for an enhanced photocurrent generation for a LPAL above 284 nm, which is not observed when using thinner active layer thicknesses. Simulating the optical characteristic of planar and patterned devices through a finite difference time domain approach proves for an increased light absorption in presence of a patterned electrode interface, originating from the excitation of propagating surface plasmon and dielectric waveguide modes. Evaluation of the external quantum efficiency characteristic and the voltage dependent charge extraction characteristics of fabricated planar and patterned solar cells reveals, however, that the increased photocurrents of patterned devices do not stem from an optical enhancement but from an improved charge carrier extraction efficiency in the space charge limited extraction regime. Presented findings clearly demonstrate that the improved charge extraction efficiency of patterned solar cells is linked to the periodic surface corrugation of the back electrode interfac

Surface plasmon resonance-enhanced light interaction in an integrated ormocomp nanowire

An integrated ormocomp nanowire coated with gold metal layer is proposed and its optical characteristics with the effect of surface plasmon resonance (SPR) are studied. The integrated rib-like nanowire has a trapezoidal shape with sidewall angles of 75°. It is coated with 50 nm gold layer to introduce the SPR and enhance the evanescent field in the sensing region located at the dielectric/metal interface. The possible field modes, the normalized power confinement, and the SPR peak position of the nanowire are studied over the wavelength and the metal thickness by using the full-vectorial H-field FEM in quasi-TM mode. The attenuation coefficient of the nanowire, the SPR peak wavelength, and the wavelength shift is experimentally extracted for three different cladding materials. The redshift of the supermode coupling between the dielectric mode and the anti-symmetric supermode is observed with the higher cladding-index and larger metal thickness. The improvement of the power confinement in the sensing region with the SPR effect is ten times (10×) better than a previous similar study

Results from an Operational Demonstration of a Gridded CrIS/ATMS Product for Cold Air Aloft

No abstract availabl

898 The impact of micrometastases in cervical cancer patients – a retrospective study of the SCCAN (Surveillance in Cervical CANcer) project

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Angle-Tunable Enhanced Infrared Reflection Absorption Spectroscopy via Grating-Coupled Surface Plasmon Resonance

Surface enhanced infrared absorption (SEIRA) spectroscopy is an attractive method for increasing the prominence of vibrational modes in infrared spectroscopy. To date, the majority of reports associated with SEIRA utilize localized surface plasmon resonance from metal nanoparticles to enhance electromagnetic fields in the region of analytes. Limited work has been performed using propagating surface plasmons as a method for SEIRA excitation. In this report, we demonstrate angle-tunable enhancement of vibrational stretching modes associated with a thin poly(methyl methacrylate) (PMMA) film that is coupled to a silver-coated diffraction grating. Gratings are fabricated using laser interference lithography to achieve precise surface periodicities, which can be used to generate surface plasmons that overlap with specific vibrational modes in the polymer film. Infrared reflection absorption spectra are presented for both bare silver and PMMA-coated silver gratings at a range of angles and polarization states. In addition, spectra were obtained with the grating direction oriented perpendicular and parallel to the infrared source in order to isolate plasmon enhancement effects. Optical simulations using the rigorous coupled-wave analysis method were used to identify the origin of the plasmon-induced enhancement. Angle-dependent absorption measurements achieved signal enhancements of more than 10-times the signal in the absence of the plasmon.This article is from Analytical Chemistry86 (2014): 2610-2617, doi:10.1021/ac4038398. Posted with permission.</p

Экспериментальное изучение влияния глюкозамина гидрохлорида на развитие патоспермии стареющих крыс, вызванной доксорубицином

ГЛЮКОЗАМИНА ГИДРОХЛОРИДДОКСОРУБИЦИНПАТОСПЕРМИЯСТАРЕНИЕФАРМАКОЛОГИЯ КЛИНИЧЕСКАЯЛЕКАРСТВ ФИЗИОЛОГИЧЕСКОЕ ДЕЙСТВИЕРЕПРОДУКЦИЮ КОНТРОЛИРУЮЩИЕ СРЕДСТВАЭКСПЕРИМЕНТЫ НА ЖИВОТНЫХКРЫСЫЦель работы - исследование влияния глюкозамина гидрохлорида на развитие гипофункции семенников крыс, вызванной длительным введением доксорубицина на фоне старения животных