Kejadian Hujan Asam di Kabupaten Bogor dan Retensi Timbal pada Domba Lokal yang Diberi Ransum Berkadar Timbal Tinggi

The objectives of this research were: 1) to find out whether acid rain has taken place in Bogor Regency area and its effect on the lead (Pb) content in soil and roughage in the region, 2) to predict the correlation of Pb content in rain water and soil, and the content of Pb in roughage in the Bogor regency area, 3) to study the impact of acid and Pb content in the ration to sheep daily gain and measure Pb content in liver, kidney and meat. The results showed that in Bogor regency area the acid rain occurred during the dry season but not in the wet season. The Pb content in the rainwater and soil were not affected by acid rain. There was no correlation of Pb content between soil and roughage. Acid containing ration decreased daily gain, but not for the ration of high Pb-content. Acid containing ration increased Pb content in the kidney but not in the liver and meat. However, high Pb content in the ration increased the Pb content in the liver and kidney but not in the meat. Key words: acid rain, Pb, Bogor, local shee

Improving pulse crops as a source of protein, starch and micronutrients

Pulse crops have been known for a long time to have beneficial nutritional profiles for human diets but have been neglected in terms of cultivation, consumption and scientific research in many parts of the world. Broad dietary shifts will be required if anthropogenic climate change is to be mitigated in the future, and pulse crops should be an important component of this change by providing an environmentally sustainable source of protein, resistant starch and micronutrients. Further enhancement of the nutritional composition of pulse crops could benefit human health, helping to alleviate micronutrient deficiencies and reduce risk of chronic diseases such as type 2 diabetes. This paper reviews current knowledge regarding the nutritional content of pea (Pisum sativum L.) and faba bean (Vicia faba L.), two major UK pulse crops, and discusses the potential for their genetic improvement

Kecernaan Nutrien dan Status Fisiologis Domba Akhir Kebuntingan dengan Ransum Flushing Minyak Sawit dan Minyak Lemuru: Nutrient Digestibility and Physiological Status of Late Pregnancy Ewe fed with Flushing Diet Containing Palm Oil and Lemuru Oil

The objective of this study was to evaluate nutrient digestibility and physiological status of local sheep at late pregnancy fed with flushing diet containing palm oil, lemuru oil, and their combination. In this research, the livestock used were 12 female local sheep at late pregnancy with an average body weight of 43.58 ± 5.57 kg were placed in metabolic cages. This study used a randomized block design (RBD) with 4 treatments and 3 block replications. The treatments were P1 = control ration (30% grass + 70% concentrate), P2 = control ration + 6% palm oil supplementation, P3 = control ration + 3% lemuru oil and 3% palm oil supplementation, P4 = control ration + 6% lemuru oil supplementation. The feed consumption, nutrient digestibility, and physiological status were measured in this study. The data obtained were analyzed using ANOVA. Means values differences were analyzed using Duncan test. The results showed that the addition of palm oil and lemuru oil to the concentrate diet increased (p<0.05) crude fat consumption, dry matter digestibility, and crude fat digestibility, but decreased (p<0.05) Nitrogen free extract consumption. Addition of palm oil and lemuru oil on the concentrate diet had no significant effect on dry matter consumption, crude protein consumption, crude fiber consumption, crude protein digestibility, crude fiber digestibility, Nitrogen free extract digestibility, and TDN value. The treatments did not affect significantly  on physiological status. It can be concluded that the use of 6% palm oil in the ration can increase crude fat consumption and crude fat digestibility in pregnant ewes. Key words:        digestibility, ewes, flushing, oil, physiological statu

Kejadian Hujan Asam di Kabupaten Bogor dan Retensi Timbal pada Domba Lokal yang Diberi Ransum Berkadar Timbal Tinggi

The objectives of this research were: 1) to find out whether acid rain has taken place in Bogor Regency area and its effect on the lead (Pb) content in soil and roughage in the region, 2) to predict the correlation of Pb content in rain water and soil, and the content of Pb in roughage in the Bogor regency area, 3) to study the impact of acid and Pb content in the ration to sheep daily gain and measure Pb content in liver, kidney and meat. The results showed that in Bogor regency area the acid rain occurred during the dry season but not in the wet season. The Pb content in the rainwater and soil were not affected by acid rain. There was no correlation of Pb content between soil and roughage. Acid containing ration decreased daily gain, but not for the ration of high Pb-content. Acid containing ration increased Pb content in the kidney but not in the liver and meat. However, high Pb content in the ration increased the Pb content in the liver and kidney but not in the meat

Brenda Garand: Northern Fiction 003

This exhibition, which was featured in the Robert and Elaine Stein Galleries at Wright State University, features works by Brenda Garand. These works combined elements of physical labor and farming culture with the skills she learned in her childhood. This exhibition ran from September 4 to October 12 2007.https://corescholar.libraries.wright.edu/restein_exhibitions_all/1123/thumbnail.jp

Phylogenetic relationship within genus <i>Lens</i>.

<p>(A) Dendrogram generated using Neighbour-Joining model based on results from first GBS library. (B) A maximum-likelihood tree based on combined results from two GBS libraries.</p

Detailed steps performed by automated GBS pipeline.

<p>(A) De-multiplex samples: Raw paired-end Illumina reads are assigned to a sample using barcode sequences, which are subsequently trimmed. (B) Trim and filter reads: De-multiplexed paired-end reads are trimmed for base quality and Illumina adaptors. (C) Align reads to a reference genome. (D) Raw SNP calls: Every position in each sample’s alignment is scanned to determine the probability of a variant.</p

Classification and Characterization of Species within the Genus <i>Lens</i> Using Genotyping-by-Sequencing (GBS)

<div><p>Lentil (<i>Lens culinaris</i> ssp. <i>culinaris</i>) is a nutritious and affordable pulse with an ancient crop domestication history. The genus <b>Lens</b> consists of seven taxa, however, there are many discrepancies in the taxon and gene pool classification of lentil and its wild relatives. Due to the narrow genetic basis of cultivated lentil, there is a need towards better understanding of the relationships amongst wild germplasm to assist introgression of favourable genes into lentil breeding programs. Genotyping-by-sequencing (GBS) is an easy and affordable method that allows multiplexing of up to 384 samples or more per library to generate genome-wide single nucleotide Polymorphism (SNP) markers. In this study, we aimed to characterize our lentil germplasm collection using a two-enzyme GBS approach. We constructed two 96-plex GBS libraries with a total of 60 accessions where some accessions had several samples and each sample was sequenced in two technical replicates. We developed an automated GBS pipeline and detected a total of 266,356 genome-wide SNPs. After filtering low quality and redundant SNPs based on haplotype information, we constructed a maximum-likelihood tree using 5,389 SNPs. The phylogenetic tree grouped the germplasm collection into their respective taxa with strong support. Based on phylogenetic tree and STRUCTURE analysis, we identified four gene pools, namely <i>L</i>. <i>culinaris/L</i>. <i>orientalis/L</i>. <i>tomentosus</i>, <i>L</i>. <i>lamottei/L</i>. <i>odemensis</i>, <i>L</i>. <i>ervoides</i> and <i>L</i>. <i>nigricans</i> which form primary, secondary, tertiary and quaternary gene pools, respectively. We discovered sequencing bias problems likely due to DNA quality and observed severe run-to-run variation in the wild lentils. We examined the authenticity of the germplasm collection and identified 17% misclassified samples. Our study demonstrated that GBS is a promising and affordable tool for screening by plant breeders interested in crop wild relatives.</p></div