324 research outputs found
La comunicación en las sedes webs de las universidades a distancia españolas
Esta investigación analiza la comunicación de las universidades españolas a distancia en sus sedes webs. En los últimos años este tipo de universidades se han multiplicado por cuatro.
Hoy en dĂa, las sedes webs de las universidades españolas online se ha convertido en una herramienta primordial para comunicar y atraer a pĂşblicos potenciales. Es más, en este tipo de universidades la sede web es el medio por el que los alumnos conocen y establecen una relaciĂłn con la universidad.
El fin de este estudio es profundizar en la forma en que las universidades a distancia se comunican a travĂ©s de sus sedes webs. Para ello, este trabajo ha utilizado el análisis de contenidos con el fin de estudiar las sedes webs de las universidades españolas a distancia.Universidad de Málaga. Campus de Excelencia Internacional AndalucĂa Tech
Estudio de tumores de la cavidad nasal y senos paranasales del perro mediante tomografĂa computarizada
En 8 perros se estudiaron imágenes de tomografĂa computarizada (TC), analizando la extensiĂłn de tumores de cavidad nasal y/o senos paranasales. La tĂ©cnica resultĂł especialmente Ăştil en animales con descarga nasal crĂłnica, protrusiĂłn del globo ocular, ceguera de origen central o signos de disfunciĂłn neurolĂłgica por afectaciĂłn de porciones rostrales del encĂ©falo.
Rv2577 of Mycobacterium tuberculosis is a virulence factor with dual phosphatase and phosphodiesterase functions
Tuberculosis, a lung disease caused by Mycobacterium tuberculosis (Mtb), is one of the ten leading causes of death worldwide affecting mainly developing countries. Mtb can persist and survive inside infected cells through modulation of host antibacterial attack, i.e., by avoiding the maturation of phagosome containing mycobacteria to more acidic endosomal compartment. In addition, bacterial phosphatases play a central role in the interplay between host cells and Mtb. In this study, we characterized the Rv2577 of Mtb as a potential alkaline phosphatase/phosphodiesterase enzyme. By an in vitro kinetic assay, we demonstrated that purified Rv2577 expressed in Mycobacterium smegmatis displays both enzyme activities, as evidenced by using the artificial substrates p-NPP and bis-(p-NPP). In addition, a three-dimensional model of Rv2577 allowed us to define the catalytic amino acid residues of the active site, which were confirmed by site-directed mutagenesis and enzyme activity analysis, being characteristic of a member of the metallophosphatase superfamily. Finally, a mutation introduced in Rv2577 reduced the replication of Mtb in mouse organs and impaired the arrest of phagosomes containing mycobacteria in early endosomes; which indicates Rv2577 plays a role in Mtb virulence.Instituto de BiotecnologĂaFil: Forrellad, Marina Andrea. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de AgrobiotecnologĂa y BiologĂa Molecular; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Blanco, Federico Carlos. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de AgrobiotecnologĂa y BiologĂa Molecular; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Marrero Diaz de Villegas, RubĂ©n. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de AgrobiotecnologĂa y BiologĂa Molecular; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Vazquez, Cristina Lourdes. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de AgrobiotecnologĂa y BiologĂa Molecular; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Yaneff, AgustĂn. Universidad de Buenos Aires. Instituto de Investigaciones FarmacolĂłgicas; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Garcia, Elizabeth Andrea. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de AgrobiotecnologĂa y BiologĂa Molecular; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Gutierrez, Maximiliano Gabriel. The Francis Crick Institute, Host-Pathogen Interactions in Tuberculosis Laboratory; Reino UnidoFil: Durán, Rosario. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones BiolĂłgicas Clemente Estable; UruguayFil: Villarino, Andrea. Universidad de la RepĂşblica (UdelaR). Facultad de Ciencias. SecciĂłn BioquĂmica; UruguayFil: Bigi, Fabiana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de AgrobiotecnologĂa y BiologĂa Molecular; Argentina. Consejo Nacional de investigaciones CientĂficas y TecnolĂłgicas; Argentin
Rv2577 of mycobacterium tuberculosis Is a virulence factor with dual phosphatase and phosphodiesterase functions
Tuberculosis, a lung disease caused by Mycobacterium tuberculosis (Mtb), is one of the ten leading causes of death worldwide affecting mainly developing countries. Mtb can persist and survive inside infected cells through modulation of host antibacterial attack, i.e., by avoiding the maturation of phagosome containing mycobacteria to more acidic endosomal compartment. In addition, bacterial phosphatases play a central role in the interplay between host cells and Mtb. In this study, we characterized the Rv2577 of Mtb as a potential alkaline phosphatase/phosphodiesterase enzyme. By an in vitro kinetic assay, we demonstrated that purified Rv2577 expressed in Mycobacterium smegmatis displays both enzyme activities, as evidenced by using the artificial substrates p-NPP and bis-(p-NPP). In addition, a three-dimensional model of Rv2577 allowed us to define the catalytic amino acid residues of the active site, which were confirmed by site-directed mutagenesis and enzyme activity analysis, being characteristic of a member of the metallophosphatase superfamily. Finally, a mutation introduced in Rv2577 reduced the replication of Mtb in mouse organs and impaired the arrest of phagosomes containing mycobacteria in early endosomes; which indicates Rv2577 plays a role in Mtb virulence
Recovery of Meteorological Data for the Observatory of A Guarda, Spain
We herein describe the recovery of a series of data on temperature, humidity, precipitation, evaporation, wind, and local weather conditions from documentary sources obtained from the Jesuit observatory of A Guarda (Galicia, Spain) for the period 1881–1896. The data were digitized and made available in accessible electronic formats. Comparisons were made with present-day meteorological data obtained from two nearby stations. We further believe that the discovery of some new complementary documentary sources made during the present research could be a basis for future data recovery efforts. Among these new results, early ozone data from the period are of outstanding importance to meteorologists
N7-methylguanosine methylation of tRNAs regulates survival to stress in cancer
Tumour progression and therapy tolerance are highly regulated and complex processes largely dependent on the plasticity of cancer cells and their capacity to respond to stress. The higher plasticity of cancer cells highlights the need for identifying targetable molecular pathways that challenge cancer cell survival. Here, we show that
- …