Eurasian Kurgan Database – a citizen science tool for conserving grasslands on historical sites

Eurasian steppes have an essential role in conserving biodiversity, but due to the huge habitat loss in the past centuries they are often preserved only in small refuges. Among such refuges are the ancient steppic burial mounds (the so called ‘kurgans’) which have a high cultural and historical importance and are also essential sites of nature conservation. Despite their high number (approximately half million) and conservational importance there is a huge lack of knowledge on the locality and conservational state of the kurgans in most regions of Eurasia. To fill this knowledge gap, we built a public database which allows to record and query basic information on their cultural values and factors (such as land cover type, threatening factors, cover of woody species) that might serve as a basis for their effective conservation. The database provides a transparent, public and easy-to-use source for conservation managers and landscape planners focussed on grassland conservation. In addition, it also provides background information for other associate disciplines and public agencies dealing with the protection of cultural heritage

Rapid preparation of pharmaceutical co-crystals with thermal ink-jet printing

Thermal ink-jet printing (TIJP) is shown to be a rapid (minutes) method with which to prepare pharmaceutical co-crystals; co-crystals were identified in all cases where the co-formers could be dissolved in water and/or water/ethanol solutions

Eurasian Kurgan Database – a citizen science tool for conserving grasslands on historical sites

Eurasian steppes have an essential role in conserving biodiversity, but due to the huge habitat loss in the past centuries they are often preserved only in small refuges. Among such refuges are the ancient steppic burial mounds (the so called ‘kurgans’) which have a high cultural and historical importance and are also essential sites of nature conservation. Despite their high number (approximately half million) and conservational importance there is a huge lack of knowledge on the locality and conservational state of the kurgans in most regions of Eurasia. To fill this knowledge gap, we built a public database which allows to record and query basic information on their cultural values and factors (such as land cover type, threatening factors, cover of woody species) that might serve as a basis for their effective conservation. The database provides a transparent, public and easy-to-use source for conservation managers and landscape planners focussed on grassland conservation. In addition, it also provides background information for other associate disciplines and public agencies dealing with the protection of cultural heritage

Mitochondria Express α7 Nicotinic Acetylcholine Receptors to Regulate Ca2+ Accumulation and Cytochrome c Release: Study on Isolated Mitochondria

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that mediate synaptic transmission in the muscle and autonomic ganglia and regulate transmitter release in the brain. The nAChRs composed of α7 subunits are also expressed in non-excitable cells to regulate cell survival and proliferation. Up to now, functional α7 nAChRs were found exclusively on the cell plasma membrane. Here we show that they are expressed in mitochondria and regulate early pro-apoptotic events like cytochrome c release. The binding of α7-specific antibody with mouse liver mitochondria was revealed by electron microscopy. Outer membranes of mitochondria from the wild-type and β2−/− but not α7−/− mice bound α7 nAChR-specific antibody and toxins: FITC-labeled α-cobratoxin or Alexa 555-labeled α-bungarotoxin. α7 nAChR agonists (1 µM acetylcholine, 10 µM choline or 30 nM PNU-282987) impaired intramitochondrial Ca2+ accumulation and significantly decreased cytochrome c release stimulated with either 90 µM CaCl2 or 0.5 mM H2O2. α7-specific antagonist methyllicaconitine (50 nM) did not affect Ca2+ accumulation in mitochondria but attenuated the effects of agonists on cytochrome c release. Inhibitor of voltage-dependent anion channel (VDAC) 4,4′-diisothio-cyano-2,2′-stilbene disulfonic acid (0.5 µM) decreased cytochrome c release stimulated with apoptogens similarly to α7 nAChR agonists, and VDAC was co-captured with the α7 nAChR from mitochondria outer membrane preparation in both direct and reverse sandwich ELISA. It is concluded that α7 nAChRs are expressed in mitochondria outer membrane to regulate the VDAC-mediated Ca2+ transport and mitochondrial permeability transition

Bats, Bat Flies, and Fungi: Exploring Uncharted Waters

Bats serve as hosts to many lineages of arthropods, of which the blood-sucking bat flies (Nycteribiidae and Streblidae) are the most conspicuous. Bat flies can in turn be parasitized by Laboulbeniales fungi, which are biotrophs of arthropods. This is a second level of parasitism, hyperparasitism, a severely understudied phenomenon. Four genera of Laboulbeniales are known to occur on bat flies, Arthrorhynchus on Nycteribiidae in the Eastern Hemisphere, Dimeromyces on Old World Streblidae, Gloeandromyces on New World Streblidae, and Nycteromyces on Streblidae in both hemispheres. In this chapter, we introduce the different partners of the tripartite interaction and discuss their species diversity, ecology, and patterns of specificity. We cover parasite prevalence of Laboulbeniales fungi on bat flies, climatic effects on parasitism of bat flies, and coevolutionary patterns. One of the most important questions in this tripartite system is whether habitat has an influence on parasitism of bat flies by Laboulbeniales fungi. We hypothesize that habitat disturbance causes parasite prevalence to increase, in line with the “dilution effect.” This can only be resolved based on large, non-biased datasets. To obtain these, we stress the importance of multitrophic field expeditions and international collaborations