In Vitro Cytotoxicity Of Calcium Silicate-Based Endodontic Cement As Root-End Filling Materials

The aim of this study was to evaluate the cytotoxicity of three types of calcium silicate-based endodontic cement after different incubation periods with human periodontal ligament fibroblasts. Human periodontal ligament fibroblasts were cultured from extracted third molars and seeded in 96-well plates. MTA, calcium enriched mixture (CEM) cement, and Biodentine were prepared and added to culture insert plates which were immediately placed into 96-well plates containing cultured cells. After incubation periods of 24, 48, and 72 hours, cell viability was determined with WST-1 assay. Data were analysed statistically by ANOVA with repeated measures and Bonferroni tests. There was no significant difference in cell viability amongst the test materials after each incubation period (P > 0.05). MTA and CEM presented more than 90% cell viability after 24 and 48 hours of incubation and showed statistically significant decrease in cell viability after 72 hours of incubation (P < 0.05). Biodentine showed significantly less cell viability (73%) after 24 hours of incubation, whereas more than 90% cell viability was seen after 48 and 72 hours of incubation (P < 0.05). Despite the significant changes in cell viability over time, materials presented similar cytotoxicity profile. Biodentine and CEM can be considered as alternative materials for root-end surgery procedures.PubMedScopu

The evaluation of the distribution of CD133, CXCR1 and the tumor associated macrophages in different molecular subtypes of breast cancer

Breast cancer has different molecular subtypes, which determine the prognosis and response to the treatment. CD133 is a marker for cancer stem cells in tumor microenvironment with diagnostic/therapeutic importance. The tumor associated macrophages (TAMs) interact with the cancer stem cells through the CXCR1 receptor. In this study, we wanted to investigate the expression of these markers in patients with different molecular subtypes, in order to detect pathophysio- logical mechanisms and new molecular targets for the prospective targeted therapies. In this study we hypothesized a difference in expression of these antigens among different subtypes. We investigated expression of antigens in breast cancer patients with luminal A (LA), luminal B (LB), HER2 overexpressing (HER2OE), triple negative (TN) subtypes (n=70) and control patients (n=10) without cancer diagnosis. We applied indirect immunohistochemistry and evaluated immunostaining. CD133 expression was at the periphery and CXCR1 expression was at the central area of the tumor. The cytoplasmic CXCR1, CD133 expressions and nuclear CD133 expression, which is prominent in the TN subtype, were observed in patients. There was a statistically significant difference between the groups for CD133 (p=0.004), CXCR1 (p=0.002) H-Score values and M2 macrophages/whole TAM ratios (p=0.022). Between the CD133 and CXCR1 H-scores, there was a weak positive correlation (r=0.249, p=0.035). This study showed the compartment specific expression of the CD133 and CXCR1 antigens in neoplastic cells. The use of CD133 as a stem cell marker may be limited to TN subtype, due to its heterogeneous expressio

Sıçanlarda İskemiy le İndüklenen Bey in Hasarı Üzerine İntravenöz ve İntrasisternal Deksmedetomidinin Etkileri: Karşılaştırmalı Bir Çalışma

AMAÇ: Sıçanlarda inkomplet serebral iskemisi üzerine intrasisternal yolla verilen deksmedetomidinin etkilerinin intravenöz yolla verilen deksmedetomidinin etkileriyle karşılaştırılması amaçlandı. YÖNTEM ve GEREÇLER: Ortak sağ karotid arter oklüzyonu ve hemoraj ik hipotansiyon ile 30 dakika süreyle serebral iskemi oluşturuldu. İntrasisternal gruplarda, iskemiden 30 dakika önce sisterna magna içine 0,1 ml % 0.9 NaCl (Grup SIC, n6) veya 9 µg/kg deksmedetomidin (Grup DIC, n6) verildi.İntravenöz gruplarda ise 9 µg/kg deksmedetomidin (Grup DIV, n6) veya % 0,9 NaCl (Grup CONTROL, n6) 5 ml/kg/h hızla 2 saat süreyle infüze edildi. Yirmi dört saat sonra beyin dokusunda ve plazmada lipid peroksidasyon seviyeleri ölçüldü. Histopatoloj ik incelemeler için hipokampal yapı kullanıldı. BULGULAR: İntravenöz deksmedetomidin ortalama arteriyel basınç ve plazma glukoz seviyelerinde bazale göre bir düşüşe neden oldu. Grup DIV ile Grup DIC, Grup SIC ve CONTROL grupları arasında beyin dokusu lipid peroksidasyon seviyeleri ve piknotik hücre sayıları bakımından anlamlı farklılık vardı (sırasıyla; p0,001, p0,001, p0,001 ve p0,001, p0,01, p0,009). Grup DIV için ortalama plazma lipid peroksidasyon seviyeleri Grup DICınkinden farklı bulundu (p0,003). SONUÇ: İskeminin indüklediği nöronal hasarda sistemik yolla verilen deksmedetomidinin nöroprotektif etkisi varken santral yolla verilen deksmedetomidinin nöroprotektif etkisi yoktur.AIM: To compare the effect of dexmedetomidine administered by intracisternal route with by intravenous route on brain tissue of rat after incomplete cerebral ischemia. MATERIAL and METHODS: Cerebral ischemia was produced by the combination of right common carotid artery occlusion and hemorrhagic hypotension during 30 minutes. Thirty minutes before the ischemia, 0.1 ml 0.9% NaCl (Group SIC, n6) or 9 µg/kg dexmedetomidine (Group DIC, n6) was administered into the cisterna magna. For the intravenous groups, 9 µg/kg dexmedetomidine (Group DIV, n6) or 0.9% NaCl (Group CONTROL, n6) 5 ml/kg/h was given in 2 hours. After 24 hours, the lipid peroxidation levels were measured in the brain tissue and plasma. Hippocampal formations were used for histopathological examination. results: Intravenous dexmedetomidine produced a decrease in baseline mean arterial blood pressure and plasma glucose concentrations. There was a significant difference between the DIV group and DIC, SIC, CONTROL groups regarding the brain lipid peroxidation levels (p<0.001, p<0.001, p0.001, respectively), and regarding the picnotic neuronal cell count (p<0.001, p0.01, p0.009, respectively). Mean plasma lipid peroxidation levels of the DIV group was different from the DIC group (p0.003). CONCLUSION: Systemically administered dexmedetomidine had neuroprotective effect in ischemia-induced neuronal damage, but centrally administered dexmedetomidine did not

Epidermal growth factor-containing wound closure enhances wound healing in non-diabetic and diabetic rats

Background: This study was designed to elucidate the in vivo efficacy of epidermal growth factor (EGF) on wound healing in non diabetic and diabetic rats

Investigation of the Possible Protective Effects of Ketamine and Dantrolene on the Hippocampal Apoptosis and Spatial Learning in Rats Exposed to Repeated Electroconvulsive Seizures as a Model of Status Epilepticus

AIM: To evaluate the possible neuroprotective effects of ketamine and dantrolene on the hippocampal apoptosis and spatial learning in rats exposed to repeated electroconvulsive seizures (ECS) as a model of status epilepticus (SE)

Histology Laboratory Guide Book

Histology and Embryology education and research are fundamental cornerstones of medical science. Histology, which is the basis of many fields of preclinical and clinical medicine, defines the visual components of cells, tissues and organs at the microscope level. This book, which we have published in the third extended version, provides students with a selection of micrographs created from archive preparations that Hacettepe University Faculty of Medicine Department of Histology and Embryology has been carefully created for more than 50 years. This selection has been prepared with the common effort of our academic staff, research assistants and technicians. The purpose of the book is to guide medical and health sciences students while examining the original preparations under the microscope in the laboratory, to match the functional properties associated with the structure of healthy cells with histochemical staining properties. Each chapter in the book contains brief information of micrographs on the basis of organs, tissues and cells under the relevant technical title. In this edition; we would respectfully like to thank the founder of our department and the first woman dean of medical school of our country, Prof. İlhan Kerse and, all of our retired professors who greatly contributed to our education by showing us the way of science. We also thank our former heads of department Prof. Ülken Örs, Prof. Esin Aşan, Prof. Ayşe Nur Çakar, Prof. Attila Dağdeviren, Prof. Sevda Fatma Müftüoğlu and Prof. Fevziye Figen Kaymaz. On behalf of our department, we wish all our students sincere success.Histoloji ve Embriyoloji, tıp biliminin vazgeçilmez temel taşlarından birisini oluşturur. Tıbbın birçok preklinik ve klinik alanına esas oluşturan histoloji, hücre, doku ve organların mikroskop düzeyinde görsel bileşenlerini tanımlar. Beşinci genişletilmiş basımını yaptığımız bu kitap, Hacettepe Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı’nın 50 yılı aşkın süredir özenle oluşturduğu arşiv preparatlarından derlenmiş mikrograf seçkisini öğrencilere sunmaktadır. Bu seçki, anabilim dalımız öğretim üyeleri, araştırma görevlileri ve teknisyenlerinin ortak emeği ile hazırlanmıştır. Kitabın amacı; tıp ve sağlık bilimleri öğrencilerine laboratuvarda bu preparatları mikroskop altında incelerken rehberlik etmek, sağlıklı hücrelerin yapıları ile ilişkili işlevsel özelliklerini, histokimyasal boyanma özellikleriyle eşleştirmelerini sağlamaktır. Kitaptaki her bölüm, ilgili teknik başlık ya da sisteme ait preparat mikrografları hakkında organ, doku, hücre temelinde kısa bilgiler vermektedir. Bu baskıda; anabilim dalımızın kurucusu ve ülkemizin ilk kadın tıp fakültesi dekanı Sn. Prof. Dr. İlhan Kerse başta olmak üzere, bizlere bilimin yolunu göstererek yetişmemize katkıda bulunan anabilim dalı başkanlarımız Sn. Prof. Dr. Ülken Örs, Sn. Prof. Dr. Esin Aşan, Sn. Prof. Dr. Ayşe Nur Çakar, Sn. Prof. Dr. Attila Dağdeviren, Sn. Prof. Dr. Sevda Fatma Müftüoğlu, Sn. Prof. Dr. Fevziye Figen Kaymaz’a ve tüm emekli hocalarımıza teşekkür ediyoruz. Anabilim dalımız adına tüm öğrencilerimize içten başarı dileklerimizle