Morphological, immunohistochemical, and molecular analysis of leiomyocellular tumors of the female reproductive system

Introduction Leiomyoma with bizarre nuclei (LBN) and cellular leiomyoma (CL) are rare variants of uterine smooth muscle tumors. In diagnostic practice, LBN can be mistaken for leiomyosarcoma (LMS), while CL may mimic low grade endometrial stromal sarcoma (LG ESS). Careful evaluation of morphological features is necessary when making the diagnosis; in some borderline cases, immunohistochemical and molecular examinations may help. Literature data on molecular genetic alterations in LBN and CL is limited, but some of these tumors appear to share certain aberrations with classical leiomyomas (UL) and LMS. Aims The aim of the work is to expand the knowledge about smooth muscle tumors of the uterus, especially LBN and CL, and perform a complex morphological, immunohistochemical (IHC), and molecular evaluation of their features. The individual goals include: 1) confirmation of the hypothesized benign behaviour of LBN, 2) morphological analysis of LBN, 3) more detailed clarification of LBN tumorigenesis with a focus on the FH gene, 4) clarification of CL tumorigenesis, 5) the use of IHC FH antibody as a screening method to identify FH gene mutations, 6) the use of morphological evaluation and results of IHC examination to facilitate differential diagnostic balance between benign and malignant smooth muscle...Úvod Leiomyom s bizarními jádry (LBN) a celulární leiomyom (CL) patří mezi vzácné varianty hladkosvalových nádorů dělohy. V diagnostické praxi může u LBN dojít k záměně s leiomyosarkomem (LMS) a u CL hlavně s low grade endometriálním stromálním sarkomem (LG ESS). Při stanovení diagnózy je nutné pečlivé zhodnocení morfologických znaků, v některých hraničních případech nám může pomoct imunohistochemické a molekulární vyšetření. Literární data týkající se molekulárně genetických alterací vyskytujících se u LBN a CL jsou limitovaná, ukazuje se však, že část těchto nádorů sdílí některé aberace s klasickými leiomyomy (UL) a LMS. Cíle Cílem práce je prohloubení znalostí o hladkosvalových nádorech dělohy, zejména LBN a CL, jejich komplexní morfologické, imunohistochemické (IHC) a molekulární zhodnocení. Cíle zahrnují: 1) podpoření hypotézy benigního chování LBN, 2) morfologickou analýzu LBN, 3) bližší ozřejmění tumorigeneze LBN se zaměřením na gen FH, 4) ozřejmění tumorigeneze CL, 5) využití IHC protilátky FH jako screeningové metody k identifikací mutací genu FH, 6) využití morfologického zhodnocení a výsledků IHC vyšetření k usnadnění diferenciálně diagnostické rozvahy mezi benigními a maligními hladkosvalovými nádory, a dále mezi nádory jiné histogeneze, 7) IHC analýzu prognosticko-prediktivních...Institute of Pathology First Faculty of Medicine Charles University and General University Hospital in PragueÚstav patologie 1. LF UK a VFN1. lékařská fakultaFirst Faculty of Medicin

Proizvodnja i percepcija govora

Zbornik radova okupio je 57 domaćih i inozemnih autora/ica, koji/e kroz 33 rada, iz različitih istraživačkih kutova, obrađuju recentne teme o proizvodnji i percepciji govora, te o njihovoj međuovisnosti u govornom procesu. Knjiga je posvećena profesoru Damiru Horgi povodom njegova sedamdesetog rođendana. Uz svaki rad naveden je sažetak na hrvatskom i engleskom jeziku. Zbornik je objavljen u suizdavaštvu Odsjeka za fonetiku Filozofskog fakulteta Sveučilišta u Zagrebu, Odjela za fonetiku Hrvatskoga filološkog društva i FF-pressa.Zbornik radova okupio je 57 domaćih i inozemnih autora/ica, koji/e kroz 33 rada, iz različitih istraživačkih kutova, obrađuju recentne teme o proizvodnji i percepciji govora, te o njihovoj međuovisnosti u govornom procesu. Knjiga je posvećena profesoru Damiru Horgi povodom njegova sedamdesetog rođendana. Uz svaki rad naveden je sažetak na hrvatskom i engleskom jeziku. Zbornik je objavljen u suizdavaštvu Odsjeka za fonetiku Filozofskog fakulteta Sveučilišta u Zagrebu, Odjela za fonetiku Hrvatskoga filološkog društva i FF-pressa

Proizvodnja i percepcija govora

Zbornik radova okupio je 57 domaćih i inozemnih autora/ica, koji/e kroz 33 rada, iz različitih istraživačkih kutova, obrađuju recentne teme o proizvodnji i percepciji govora, te o njihovoj međuovisnosti u govornom procesu. Knjiga je posvećena profesoru Damiru Horgi povodom njegova sedamdesetog rođendana. Uz svaki rad naveden je sažetak na hrvatskom i engleskom jeziku. Zbornik je objavljen u suizdavaštvu Odsjeka za fonetiku Filozofskog fakulteta Sveučilišta u Zagrebu, Odjela za fonetiku Hrvatskoga filološkog društva i FF-pressa.Zbornik radova okupio je 57 domaćih i inozemnih autora/ica, koji/e kroz 33 rada, iz različitih istraživačkih kutova, obrađuju recentne teme o proizvodnji i percepciji govora, te o njihovoj međuovisnosti u govornom procesu. Knjiga je posvećena profesoru Damiru Horgi povodom njegova sedamdesetog rođendana. Uz svaki rad naveden je sažetak na hrvatskom i engleskom jeziku. Zbornik je objavljen u suizdavaštvu Odsjeka za fonetiku Filozofskog fakulteta Sveučilišta u Zagrebu, Odjela za fonetiku Hrvatskoga filološkog društva i FF-pressa

Morphological, immunohistochemical, and molecular analysis of leiomyocellular tumors of the female reproductive system

Introduction Leiomyoma with bizarre nuclei (LBN) and cellular leiomyoma (CL) are rare variants of uterine smooth muscle tumors. In diagnostic practice, LBN can be mistaken for leiomyosarcoma (LMS), while CL may mimic low grade endometrial stromal sarcoma (LG ESS). Careful evaluation of morphological features is necessary when making the diagnosis; in some borderline cases, immunohistochemical and molecular examinations may help. Literature data on molecular genetic alterations in LBN and CL is limited, but some of these tumors appear to share certain aberrations with classical leiomyomas (UL) and LMS. Aims The aim of the work is to expand the knowledge about smooth muscle tumors of the uterus, especially LBN and CL, and perform a complex morphological, immunohistochemical (IHC), and molecular evaluation of their features. The individual goals include: 1) confirmation of the hypothesized benign behaviour of LBN, 2) morphological analysis of LBN, 3) more detailed clarification of LBN tumorigenesis with a focus on the FH gene, 4) clarification of CL tumorigenesis, 5) the use of IHC FH antibody as a screening method to identify FH gene mutations, 6) the use of morphological evaluation and results of IHC examination to facilitate differential diagnostic balance between benign and malignant smooth muscle..

Data from: Cross-sectional study of patients with axial spondyloarthritis fulfilling imaging arm of ASAS classification criteria: baseline clinical characteristics and subset differences in a single centre cohort

Objective: This study compared demographic, clinical and laboratory characteristics between patients with radiographic and non-radiographic axSpA. Methods: In this single centre cross-sectional study a total of 246 patients with axSpA fulfilling the imaging arm of ASAS classification criteria were recruited. A total of 140 patients were diagnosed as nr-axSpA, and 106 patients had AS. Sociodemographic characteristics, disease manifestations, clinical and laboratory disease activity and their differences between subsets were analysed. P values below 0.05 with CI 95% were considered statistically significant. Results: More nr-axSpA patients were females (61.4%) compared to 24.7% of AS patients. First symptoms developed earlier in AS patients compared to nr-axSpA (23.0 (IQR 17.5-30.0) vs. 27.8 (IQR 21.0-33.7) years, p=0.001). Disease manifestations did not differ, but patients with nr-axSpA experienced peripheral arthritis more frequently (35.7% vs. 17.0%, p=0.001) with less hip involvement (8.6% vs. 18.9%, p=0.022) compared to patients with AS. Patients with AS exhibited worse spinal mobility and physical function compared to nr-axSpA. Ankylosing Spondylitis Disease Activity Scores and CRP levels were significantly higher in patients with AS compared to nr-axSpA (2.4 (IQR 1.7-2.8) vs. 2.0 (IQR 1.1-2.3), p=0.022 and 7.1 (IQR 2.6-14.9) vs. 2.5 (IQR 0.8-8.2) mg/L, p<0.001, respectively). Conclusions: Our data demonstrated some known and also novel differences between the two imaging arm fulfilling axSpA subgroups. Non-radiographic patients were mostly women who had experienced shorter disease duration, milder disease activity and better functional status with less hip involvement but more peripheral arthritis compared to patients with AS

data-dryad

AxSpA patients data for analyses and data table