2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.

In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV

Molecular characterization of Babesia vogeli in dogs from Belém, northern Brazil

Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular, Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular, Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular, Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular, Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular, Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Belém , PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular, Belém, PA, Brasil.Babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the Babesia genus (Apicomplexa). Little is known about this genus; therefore, this study conducted a molecular characterization of Babesia spp in naturally infected dogs in northern Brazil. Blood samples were collected from 172 dogs from metropolitan Belém, and screened for Babesia spp using semi-nested polymerase chain reactions. Subsequently, 18S rDNA gene fragments were amplified and sequenced. Alignments of the 27 nucleotide sequences returned fragments measuring 1023 bp in size, which did not include any polymorphic sites (100 per cent identity). This genotype was very similar to the 18S rDNA gene in B. vogeli. This study provides an important molecular characterization of the parasite responsible for canine babesiosis in naturally infected dogs in metropolitan Belém. In addition, the 18S rDNA gene in B. vogeli may represent the most common genotype occurring in South America

Natural infection by Culex flavivirus in Culex quinquefasciatus mosquitoes captured in Cuiabá, Mato Grosso Mid-Western Brazil

DECIT/SCTIE/MS, CNPq, FAPEMAT and SES-MT, process 214502/2013. CNPq grant 200024/2015-9.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiabá, MT, Brazil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiabá, MT, Brazil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiabá, MT, Brazil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiabá, MT, Brazil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiabá, MT, Brazil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiabá, MT, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiabá, MT, Brazil.New species of insect‐specific viruses (ISV) have been reported worldwide. In the present study, the complete genome of Culex flavivirus (CxFV) and partial sequences of other ISVs in Culex quinquefasciatus Say 1823 females (n = 3425) sampled in 200 urban areas census tracts of Cuiaba, state of Mato Grosso, were identified via reverse transcriptase‐polymerase chain reaction for a NS5 region of flaviviruses, nucleotide and high‐throughput sequencing, and viral isolation in C6/36 cells. CxFV was detected in 16 of 403 mosquito pools; sequences found in the study presented a high similarity with isolates from São Paulo, Brazil and other countries in Latin American that belong to genotype II, supporting the geographical influence on CxFV evolution. The monthly maximum likelihood estimation for CxFV ranged from 1.81 to 9.94 per 1000 mosquitoes. In addition to the CxFV complete genome, one pool contained an ORF1 sequence (756 bp) that belongs to a novel Negevirus from the Sandewavirus supergroup most similar to the Santana virus (77.1%) and another pool presented an RNA‐dependent RNA polymerase sequence (1081 bp) of a novel Rhabdovirus most similar to Wuhan mosquito virus 9 (44%). After three passages in C6/36 cells, only CxFV was isolated from these co‐infected pools. The importance of ISVs relies on their possible ability to interfere with arbovirus replication in competent vectors

New virus genome sequences of the Guama Serogroup (Genus Orthobunyavirus, Family Bunyaviridae), isolated in the Brazilian Amazon Region

Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.This is the first announcement of two nearly complete viral genome sequences belonging to the Guama serogroup (genus Orthobunyavirus, family Bunyaviridae) isolated in the Brazilian Amazon region: Mirim virus (MIRV; BEAN7722) and Ananindeua virus (ANUV; BEAN109303)

Full-length genomic and molecular characterization of Canine parvovirus in dogs from North of Brazil.

PIBIC/IEC/CNPq/2015-2016 and Fundação de Amparo à Pesquisa do Estado de São Paulo, Brasil (Scholarships #12/24150-9 and #15/05778-5).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto, SP, Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.Universidade Federal Rural da Amazônia. Laboratório de Biologia Molecular. Belém, PA. Brasil.With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Belém. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Belém CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Belém originated from CPV-2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV-2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil

Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic.

Contains fulltext : 108030.pdf (publisher's version ) (Open Access)The 7th cholera pandemic reached Latin America in 1991, spreading from Peru to virtually all Latin American countries. During the late epidemic period, a strain that failed to ferment sucrose dominated cholera outbreaks in the Northern Brazilian Amazon region. In order to understand the genomic characteristics and the determinants of this altered sucrose fermenting phenotype, the genome of the strain IEC224 was sequenced. This paper reports a broad genomic study of this strain, showing its correlation with the major epidemic lineage. The potentially mobile genomic regions are shown to possess GC content deviation, and harbor the main V. cholera virulence genes. A novel bioinformatic approach was applied in order to identify the putative functions of hypothetical proteins, and was compared with the automatic annotation by RAST. The genome of a large bacteriophage was found to be integrated to the IEC224's alanine aminopeptidase gene. The presence of this phage is shown to be a common characteristic of the El Tor strains from the Latin American epidemic, as well as its putative ancestor from Angola. The defective sucrose fermenting phenotype is shown to be due to a single nucleotide insertion in the V. cholerae sucrose-specific transportation gene. This frame-shift mutation truncated a membrane protein, altering its structural pore-like conformation. Further, the identification of a common bacteriophage reinforces both the monophyletic and African-Origin hypotheses for the main causative agent of the 1991 Latin America cholera epidemics

Genetic ancestry and income are associated with dengue hemorrhagic fever in a highly admixed population.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-04-23T14:29:45Z No. of bitstreams: 1 Blanton R Genetic ancestry....pdf: 115239 bytes, checksum: 910b1e8af0e8f506e9776e1c78ae926b (MD5)Made available in DSpace on 2014-04-23T14:29:45Z (GMT). No. of bitstreams: 1 Blanton R Genetic ancestry....pdf: 115239 bytes, checksum: 910b1e8af0e8f506e9776e1c78ae926b (MD5) Previous issue date: 2008Case Western Reserve University. Center for Global Health and Diseases. Cleveland, OH, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilUniversidade Federal da Bahia. Institute of Collective Health. Salvador, BA, BrasilCase Western Reserve University. Division of Genetic Epidemiology. Department of Epidemiology & Biostatistics Wolstein Research Building. Cleveland, OH, USASecretaria da Saúde do Estado da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilCase Western Reserve University. Division of Genetic Epidemiology. Department of Epidemiology & Biostatistics Wolstein Research Building. Cleveland, OH, USAInstituto Evandro Chagas. Department of Arbovirology and Hemorrhagic Fever. Belém, PA, BrasilInstituto Evandro Chagas. Department of Arbovirology and Hemorrhagic Fever. Belém, PA, BrasilInstituto Evandro Chagas. Department of Arbovirology and Hemorrhagic Fever. Belém, PA, BrasilSecretaria da Saúde do Estado da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilUniversidade Federal da Bahia. Institute of Collective Health. Salvador, BA, BrasilUniversidade Federal da Bahia. Institute of Collective Health. Salvador, BA, BrasilTo test whether African ancestry is protective for severe dengue, we genotyped 49 hospitalized cases of dengue hemorrhagic fever (DHF) as well as 293 neighborhood cases of dengue fever and 294 asymptomatic controls in Salvador, Bahia, Brazil. Ancestry-informative markers and 282 unlinked SNPs not associated with the clinical presentation of dengue were used to estimate ancestry. After controlling for income, both self-defined Afro-Brazilian ethnicity and African ancestry were protective for DHF (P¼0.02, OR¼0.28 and P¼0.02, OR¼0.13, respectively). Income or an index of income indicators, however, was also independently associated with the diagnosis of DHF

Genome of the Latin American epidemic <i>Vibrio cholerae</i> marker phage.

<p>Graphical representation of the genes that correspond to the genome of a bacteriophage that is present in all the Latin American epidemic strains tested in this study. The CDS are the blue arrows that are pointed towards the direction they are coded in the genome. The putative protein functions are listed below, with a corresponding number to its localization in the image. (Images generated in the Geneious software – reference <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037283#pone.0037283-Drummond1" target="_blank">[24]</a>)</p

General genome features of the <i>V. cholerae</i> strain IEC224.

<p>General genome features of the <i>V. cholerae</i> strain IEC224.</p