715 research outputs found

    MicrobeDB: a locally maintainable database of microbial genomic sequences

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    Summary: Analysis of microbial genomes often requires the general organization and comparison of tens to thousands of genomes both from public repositories and unpublished sources. MicrobeDB provides a foundation for such projects by the automation of downloading published, completed bacterial and archaeal genomes from key sources, parsing annotations of all genomes (both public and private) into a local database, and allowing interaction with the database through an easy to use programming interface. MicrobeDB creates a simple to use, easy to maintain, centralized local resource for various large-scale comparative genomic analyses and a back-end for future microbial application design

    Detection of Helicobacter pylori Microevolution and Multiple Infection from Gastric Biopsies by Housekeeping Gene Amplicon Sequencing

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    Despite the great efforts devoted to research on Helicobacter pylori, the prevalence of single-strain infection or H. pylori mixed infection and its implications in the mode of transmission of this bacterium are still controversial. In this study, we explored the usefulness of housekeeping gene amplicon sequencing in the detection of H. pylori microevolution and multiple infections. DNA was extracted from five gastric biopsies from four patients infected with distinct histopathological diagnoses. PCR amplification of six H. pylori-specific housekeeping genes was then assessed on each sample. Optimal results were obtained for the cgt and luxS genes, which were selected for amplicon sequencing. A total of 11,833 cgt and 403 luxS amplicon sequences were obtained, 2042 and 112 of which were unique sequences, respectively. All cgt and luxS sequences were clustered at 97% to 9 and 13 operational taxonomic units (OTUs), respectively. For each sample from a different patient, a single OTU comprised the majority of sequences in both genes, but more than one OTU was detected in all samples. These results suggest that multiple infections with a predominant strain together with other minority strains are the main way by which H. pylori colonizes the human stomach

    Brazilian spring wheat germplasm as source of genetic variability.

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    As part of a Canada-Brazil germplasm exchange, 106 modern and ancient Brazilian spring wheat cultivars have been genotyped and phenotypically evaluated in Canada since 201

    Blueberry Progress Reports

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    The 1978 edition of the Blueberry Progress Reports was prepared for the Maine Blueberry Commission and the University of Maine Blueberry Advisory Committee by researchers with the Maine Life Sciences and Agriculture Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Weed Control in Blueberry Fields 2. Pruning of Blueberries 3. Integrated Management of Blueberry Fields 4. Factors Regulating Rhizome Initiation and Development in the Lowbush Blueberry 5. Effect of Plant-Water Stress on Lowbush Blueberry Growth Yield and Quality 6. Blossom Blight of Blueberries 7. Botrytis Blossom Blight of Lowbush Blueberries 8. Insects Affecting the Blueberry 9. Treatment of Blueberries with Potassium Sorbate to Reduce Spoilage During Temporary Storage 10. Cooperative Extension Activitie

    Blueberry Research Progress Report

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    The 1976 edition of the Blueberry Research Progress Report was prepared for the Blueberry Advisory Committee by researchers at the University of Maine, Orono. Projects in this report include: 1. Effect of Plant-Water Stress on Lowbush Blueberry Growth, Yield and Quality 2. Insects Affecting the Blueberry 3. The Development of Blueberry Varieties Adapted to Maine 4. Weed Control in Blueberry Fields 5. Pruning of Blueberries 6. Integrated Management of Blueberry Fields 7. The Physiology and Biochemistry of the Development of the Lowbush Blueberry Fruit 8. Survey of Red Leaf Disease of Blueberries 9. Blossom Blight of Blueberries 10. Blueberry Marketing and Cost Analysis of Producing Blueberries 11. Factors Regulating Rhizome Initiation and Development in the Lowbush Blueberry 12. New Food Products and Servic
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