Role of Cancer Side Population Stem Cells in Ovarian Cancer Angiogenesis

\ua9 2024 The Author(s).Ovarian cancer is one of the most common gynecologic malignancies. Recurrence and metastasis often occur after treatment, and it has the highest mortality rate of all gynecological tumors. Cancer stem cells (CSCs) are a small population of cells with the ability of self-renewal, multidirectional differentiation, and infinite proliferation. They have been shown to play an important role in tumor growth, metastasis, drug resistance, and angiogenesis. Ovarian cancer side population (SP) cells, a type of CSC, have been shown to play roles in tumor formation, colony formation, xenograft tumor formation, ascites formation, and tumor metastasis. The rapid progression of tumor angiogenesis is necessary for tumor growth; however, many of the mechanisms driving this process are unclear as is the contribution of CSCs. The aim of this review was to document the current state of knowledge of the molecular mechanism of ovarian cancer stem cells (OCSCs) in regulating tumor angiogenesis

Methodologies for the analysis of pesticides and pharmaceuticals in sediments and plant tissue

Eco-technologies that utilize natural processes involving wetland vegetation, soil and their associated microbial assemblages are increasingly used for the removal of contaminants of emerging concern (CECs) from polluted water. However, information on removal processes in these systems is not always available, possibly due to the lack of simple and robust methodologies for analysis of CECs in complex matrices such as sediment and plant tissue. The aim of the present study was to use a simple and fast procedure based on ultrasonic extraction (USE) and reduced clean-up procedures to analyse 8 pesticides and 9 pharmaceuticals by high-performance liquid chromatography (HPLC) coupled with diode array detector. The established methods demonstrated suitable sensitivity and reliability, and proved fit-for-purpose in quantifying multiple classes of pesticides and pharmaceuticals. For sediments, extraction with methanol/acetone (95:5, v/v) followed by a simple evaporation to dryness and redissolution (water:methanol 50:50) provided acceptable recovery (50 - 101%) and RSD 64%) with RSD < 22% determined using different types of wetland plants. The methodology has been successfully applied in different studies on the fate of emerging contaminants in water treatment eco-technology systems

Designing and Optimizing a Healthcare Kiosk for the Community

Investigating new ways to deliver care, such as the use of self-service kiosks to collect and monitor signs of wellness, supports healthcare efficiency and inclusivity. Self-service kiosks offer this potential, but there is a need for solutions to meet acceptable standards, e.g., provision of accurate measurements. This study investigates the design and optimization of a prototype healthcare kiosk to collect vital signs measures. The design problem was decomposed, formalized, focused and used to generate multiple solutions. Systematic implementation and evaluation allowed for the optimization of measurement accuracy, first for individuals and then for a population. The optimized solution was tested independently to check the suitability of the methods, and quality of the solution. The process resulted in a reduction of measurement noise and an optimal fit, in terms of the positioning of measurement devices. This guaranteed the accuracy of the solution and provides a general methodology for similar design problems

Response of freezing/thawing indexes to the wetting trend under warming climate conditions over the Qinghai -Tibetan Plateau during 1961–2010: A numerical simulation

Since the 1990s, the Qinghai-Tibetan Plateau (QTP) has experienced a strikingly warming and wetter climate that alters the thermal and hydrological properties of frozen ground. A positive correlation between the warming and thermal degradation in permafrost or seasonally frozen ground (SFG) has long been recognized. Still, a predictive relationship between historical wetting under warming climate conditions and frozen ground has not yet been well demonstrated, despite the expectation that it will become even more important because precipitation over the QTP has been projected to increase continuously in the near future. This study investigates the response of the thermal regime to historical wetting in both permafrost and SFG areas and examines their relationships separately using the Community Land Surface Model version 4.5. Results show that wetting before the 1990s across the QTP mainly cooled the permafrost body in the arid and semiarid zones, with significant correlation coefficients of 0.60 and 0.48, respectively. Precipitation increased continually at the rate of 6.16 mm decade−1 in the arid zone after the 1990s but had a contrasting warming effect on permafrost through a significant shortening of the thawing duration within the active layer. However, diminished rainfall in the humid zone after the 1990s also significantly extended the thawing duration of SFG. The relationship between the ground thawing index and precipitation was significantly negatively correlated (−0.75). The dual effects of wetting on the thermal dynamics of the QTP are becoming critical because of the projected increases in future precipitation. © 2023, The Author(s)

Grain boundary effects on magnetotransport in bi-epitaxial films of La0.7_{0.7}Sr0.3_{0.3}MnO3_3

The low field magnetotransport of La$_{0.7}$Sr$_{0.3}$MnO$_3$ (LSMO) films grown on SrTiO$_3$ substrates has been investigated. A high qualtity LSMO film exhibits anisotropic magnetoresistance (AMR) and a peak in the magnetoresistance close to the Curie temperature of LSMO. Bi-epitaxial films prepared using a seed layer of MgO and a buffer layer of CeO$_2$ display a resistance dominated by grain boundaries. One film was prepared with seed and buffer layers intact, while a second sample was prepared as a 2D square array of grain boundaries. These films exhibit i) a low temperature tail in the low field magnetoresistance; ii) a magnetoconductance with a constant high field slope; and iii) a comparably large AMR effect. A model based on a two-step tunneling process, including spin-flip tunneling, is discussed and shown to be consistent with the experimental findings of the bi-epitaxial films.Comment: REVTeX style; 14 pages, 9 figures. Figure 1 included in jpeg format (zdf1.jpg); the eps was huge. Accepted to Phys. Rev.

Evidence of a gate-tunable Mott insulator in a trilayer graphene moiré superlattice

The Mott insulator is a central concept in strongly correlated physics and manifests when the repulsive Coulomb interaction between electrons dominates over their kinetic energy . Doping additional carriers into a Mott insulator can give rise to other correlated phenomena such as unusual magnetism and even high-temperature superconductivity . A tunable Mott insulator, where the competition between the Coulomb interaction and the kinetic energy can be varied in situ, can provide an invaluable model system for the study of Mott physics. Here we report the possible realization of such a tunable Mott insulator in a trilayer graphene heterostructure with a moiré superlattice. The combination of the cubic energy dispersion in ABC-stacked trilayer graphene and the narrow electronic minibands induced by the moiré potential leads to the observation of insulating states at the predicted band fillings for the Mott insulator. Moreover, the insulating states in the heterostructure can be tuned: the bandgap can be modulated by a vertical electrical field, and at the same time the electron doping can be modified by a gate to fill the band from one insulating state to another. This opens up exciting opportunities to explore strongly correlated phenomena in two-dimensional moiré superlattice heterostructures. 1,2 2,3 4–8 9–1

Global divergent trends of algal blooms detected by satellite during 1982–2018

Algal blooms (ABs) in inland lakes have caused adverse ecological effects, and health impairment of animals and humans. We used archived Landsat images to examine ABs in lakes (>1 km2) around the globe over a 37-year time span (1982–2018). Out of the 176032 lakes with area >1 km2 detected globally, 863 were impacted by ABs, 708 had sufficiently long records to define a trend, and 66% exhibited increasing trends in frequency ratio (FRQR, ratio of the number of ABs events observed in a year in a given lake to the number of available Landsat images for that lake) or area ratio (AR, ratio of annual maximum area covered by ABs observed in a lake to the surface area of that lake), while 34% showed a decreasing trend. Across North America, an intensification of ABs severity was observed for FRQR (p<.01) and AR (p <.01) before 1999, followed by a decrease in ABs FRQR (p <.01) and AR (p <.05) after the 2000s. The strongest intensification of ABs was observed in Asia, followed by South America, Africa, and Europe. No clear trend was detected for the Oceania. Across climatic zones, the contributions of anthropogenic factors to ABs intensification (16.5% for fertilizer, 19.4% for gross domestic product, and 18.7% for population) were slightly stronger than climatic drivers (10.1% for temperature, 11.7% for wind speed, 16.8% for pressure, and for 11.6% for rainfall). Collectively, these divergent trends indicate that consideration of anthropogenic factors as well as climate change should be at the forefront of management policies aimed at reducing the severity and frequency of ABs in inland waters

Growth and Viability of Cutaneous Squamous Cell Carcinoma Cell Lines Display Different Sensitivities to Isoform-Specific Phosphoinositide 3-Kinase Inhibitors.

Cutaneous squamous cell carcinomas (cSCCs) account for about 20% of keratinocyte carcinomas, the most common cancer in the UK. Therapeutic options for cSCC patients who develop metastasis are limited and a better understanding of the biochemical pathways involved in cSCC development/progression is crucial to identify novel therapeutic targets. Evidence indicates that the phosphoinositide 3-kinases (PI3Ks)/Akt pathway plays an important role, in particular in advanced cSCC. Questions remain of whether all four PI3K isoforms able to activate Akt are involved and whether selective inhibition of specific isoform(s) might represent a more targeted strategy. Here we determined the sensitivity of four patient-derived cSCC cell lines to isoform-specific PI3K inhibitors to start investigating their potential therapeutic value in cSCC. Parallel experiments were performed in immortalized keratinocyte cell lines. We observed that pan PI3Ks inhibition reduced the growth/viability of all tested cell lines, confirming the crucial role of this pathway. Selective inhibition of the PI3K isoform p110α reduced growth/viability of keratinocytes and of two cSCC cell lines while affecting the other two only slightly. Importantly, p110α inhibition reduced Akt phosphorylation in all cSCC cell lines. These data indicate that growth and viability of the investigated cSCC cells display differential sensitivity to isoform-specific PI3K inhibitors