Preparation and properties of modified aqueous sodium-ion electrode material Na3V2(PO4)3

Na3V2(PO4)3(NVP) in aqueous sodium-ion batteries cathode material was prepared by hydrothermal method and doped with non-metallic N and metal ions Al and Mn to improve the electrochemical performance of NVP. The effects of doping amount on the performance modification of NVP were investigated. When the doping amount of Mn is 0.08 mol, the samples have an obvious layered structure and show the best discharge specific capacity of 439.8 F/g. The structure and morphology of NVP were characterized by XRD, SEM, BET and XPS, and the electrochemical performance of the sample was analyzed by cyclic voltammetry and charge and discharge test. The results show that all the samples are pure NVP, and ion doping does not change the crystal structure of NVP. Al doping improves the dispersion of particles, and N, Mn doping forms obvious layered structure. The discharge specific capacity of NVP is 342 F/g, and 380.8, 405, 439.8 F/g for NVP/N, NVP/Al, NVP/Mn, at a current rate of 1 A/g. Thus, it can be seen that doping with appropriate amounts of metal ions and non-metal elements can significantly improve the aqueous electrochemical performance of NVP

¿Does the Kish grid sampling method distort the structure of samples in risk factor surveillance?

Background: The Kish grid is commonly used in risk factor surveillance as a sampling method within households,. However through the risk factor surveillance surveys of Jiangsu Province of China in the years of 2007, 2010 and 2013, in which Kish grid method was used to select one eligible respondent within a household, we observed that the structure of samples was different from the population. Purpose: To determine if the Kish grid method distorts the structure of the sample in risk factor surveillance and to explore possible solution to this problem

Impact of urbanization on baseflow characteristics in the central catchment of North China Plain, China

Study area: The Dawenhe River and Xiaoqinghe River Basins (116°21′–118°41′E, 35°43′–37°15′N) in the center of the North China Plain (NCP), China. Study focus: To quantitatively assess the influence of urbanization (two indicators including Impervious Surface Percentage (ISP) and Average Night Light Index (ANLI)) on baseflow hydrological behavior (nine baseflow signatures), this study selects the typical urbanized catchment (nine sub-catchments) within the central NCP, where the baseflow was estimated from the total daily streamflow from 2006 to 2016. New hydrological insights for the region: Findings show that: (i) Baseflow signatures had a notable spatial variability, in which the magnitude baseflow signatures (Qb10, Qb25, Qb50, Qb90), Baseflow Index (BFI), Concavity Index (CI), and Slope of the baseflow duration curve (SBDC) demonstrated greater values in the north versus the south. (ii) Both ISP and ANLI exhibited an upward trend over time with some fluctuations (R2 > 0.65, p < 0.05). For ANLI, there were higher values in the northwest and northeast regions, and the higher ISP values were mainly located in the northern region. (iii) Overall, the effects of two urbanization indicators on baseflow signatures exhibited catchment-variability, and model performance between ISP and baseflow signatures is better than ANLI. This study provides a scientific reference to elucidate the water balance of the central NCP under the background of urbanization

Geographical Environment Factors and Risk Assessment of Tick-Borne Encephalitis in Hulunbuir, Northeastern China

Tick-borne encephalitis (TBE) is one of natural foci diseases transmitted by ticks. Its distribution and transmission are closely related to geographic and environmental factors. Identification of environmental determinates of TBE is of great importance to understanding the general distribution of existing and potential TBE natural foci. Hulunbuir, one of the most severe endemic areas of the disease, is selected as the study area. Statistical analysis, global and local spatial autocorrelation analysis, and regression methods were applied to detect the spatiotemporal characteristics, compare the impact degree of associated factors, and model the risk distribution using the heterogeneity. The statistical analysis of gridded geographic and environmental factors and TBE incidence show that the TBE patients mainly occurred during spring and summer and that there is a significant positive spatial autocorrelation between the distribution of TBE cases and environmental characteristics. The impact degree of these factors on TBE risks has the following descending order: temperature, relative humidity, vegetation coverage, precipitation and topography. A high-risk area with a triangle shape was determined in the central part of Hulunbuir; the low-risk area is located in the two belts next to the outside edge of the central triangle. The TBE risk distribution revealed that the impact of the geographic factors changed depending on the heterogeneity

DataSheet_1_Construction of a molecular inflammatory predictive model with histone modification-related genes and identification of CAMK2D as a potential response signature to infliximab in ulcerative colitis.docx

BackgroundUlcerative colitis (UC) is a lifelong inflammatory disease affecting the rectum and colon with numerous treatment options that require an individualized treatment plan. Histone modifications regulate chromosome structure and gene expression, resulting in effects on inflammatory and immune responses. However, the relationship between histone modification-related genes and UC remains unclear.MethodsTranscriptomic data from GSE59071 and GSE66407 were obtained from the Gene Expression Omnibus (GEO), encompassing colonic biopsy expression profiles of UC patients in inflamed and non-inflamed status. Differentially expressed gene (DEG) analyses, functional enrichment analyses, weighted gene co-expression network analysis (WGCNA), and random forest were performed to identify histone modification-related core genes associated with UC inflammation. Features were screened through the least absolute shrinkage and selection operator (LASSO) and support vector machine‐recursive feature elimination (SVM‐RFE), establishing a molecular inflammatory predictive model using logistic regression. The model was validated in the GSE107499 dataset, and the performance of the features was assessed using receiver operating characteristic (ROC) and calibration curves. Immunohistochemistry (IHC) staining of colonic biopsy tissues from UC patients treated with infliximab was used to further confirm the clinical application value. Univariate logistic regression on GSE14580 highlighted features linked to infliximab response.ResultsA total of 253 histone modification-related DEGs were identified between inflammatory and non-inflammatory patients with UC. Seven key genes (IL-1β, MSL3, HDAC7, IRF4, CAMK2D, AUTS2, and PADI2) were selected using WGCNA and random forest. Through univariate logistic regression, three core genes (CAMK2D, AUTS2, and IL-1β) were further incorporated to construct the molecular inflammatory predictive model. The area under the curve (AUC) of the model was 0.943 in the independent validation dataset. A significant association between CAMK2D protein expression and infliximab response was observed, which was validated in another independent verification set of GSE14580 from the GEO database.ConclusionThe molecular inflammatory predictive model based on CAMK2D, AUTS2, and IL-1β could reliably distinguish the mucosal inflammatory status of UC patients. We further revealed that CAMK2D was a predictive marker of infliximab response. These findings are expected to provide a new evidence base for personalized treatment and management strategies for UC patients.</p

Partial desensitization of MYC2 transcription factor alters the interaction with jasmonate signaling components and affects specialized metabolism

The activity of bHLH transcription factor MYC2, a key regulator in jasmonate signaling and plant specialized metabolism, is sensitive to repression by JASMONATE-ZIM-domain (JAZ) proteins and co-activation by the mediator subunit MED25. The substitution of a conserved aspartic acid (D) to asparagine (N) in the JAZ-interacting domain (JID) of Arabidopsis MYC2 affects interaction with JAZ, although the mechanism remained unclear. The effects of the conserved residue MYC2D128 on interaction with MED25 have not been investigated. Using tobacco as a model, we generated all possible substitutions of aspartic acid 128 (D128) in NtMYC2a. NtMYC2aD128N partially desensitized the repression by JAZ proteins, while strongly interacting with MED25, resulting in increased expression of nicotine pathway genes and nicotine accumulation in tobacco hairy roots overexpressing NtMYC2aD128N compared to those overexpressing NtMYC2a. The proline substitution, NtMYC2aD128P, negatively affected transactivation and abolished the interaction with JAZ proteins and MED25. Structural modeling and simulation suggest that the overall stability of the JID binding pocket is a predominant cause for the observed effects of substitutions at D128. The D128N substitution has an overall stabilizing effect on the binding pocket, which is destabilized by D128P. Our study offers an innovative tool to increase the production of plant natural products.Published versionThis work is supported partially by the Harold R. Burton Endowed Professorship to L.Y., and by the grants 2018530000241001 and 2022530000241012 from the Yunnan Tobacco Company, the National Key Research and Development Program of China (2019YFC1711100), the Kentucky Tobacco Research and Development Center (KTRDC), The Shandong Province Modern Agricultural Technology System (SDAIT-25- 02), The China Tobacco Shandong Industrial Corporation Major Project (202102004), The Shandong Tobacco Company Science and Technology Project (KN294, KN291, KN293, KN287), The Shandong Weifang Tobacco Company Science and Technology Project (2021-57), and The Shandong Rizhao Tobacco Company Science and Technology Project (2022-003)

De novo variants in genes regulating stress granule assembly associate with neurodevelopmental disorders

Stress granules (SGs) are cytoplasmic assemblies in response to a variety of stressors. We report a new neurodevelopmental disorder (NDD) with common features of language problems, intellectual disability, and behavioral issues caused by de novo likely gene-disruptive variants in UBAP2L, which encodes an essential regulator of SG assembly. Ubap2l haploinsufficiency in mouse led to social and cognitive impairments accompanied by disrupted neurogenesis and reduced SG formation during early brain development. On the basis of data from 40,853 individuals with NDDs, we report a nominally significant excess of de novo variants within 29 genes that are not implicated in NDDs, including 3 essential genes (G3BP1, G3BP2, and UBAP2L) in the core SG interaction network. We validated that NDD-related de novo variants in newly implicated and known NDD genes, such as CAPRIN1, disrupt the interaction of the core SG network and interfere with SG formation. Together, our findings suggest the common SG pathology in NDDs