Genome-wide transcriptomics analysis identifies sox7 and sox18 as specifically regulated by gata4 in cardiomyogenesis

This work was supported by British Heart Foundation (BHF Project Grant no PG/13/23/30080 to B.A.A and S.H.), Biotechnology and Biological Sciences Research Council (BB/M001695/1 to S.H.) and the University of Aberdeen (for A.T.L). Acknowledgements We’re grateful to Ms Yvonne Turnbull and Ms Kate Watt for technical assistance and lab management. We would like to thank Professor Cedric Blanpain and Dr Xionghui Li from Université Libre de Bruxelles for providing training of ES cell manipulation and Mesp1/Gata4 cell lines. We are grateful to Professor Todd Evans from Weill Cornell Medical College for generously providing iGata ES cell lines. We also would like to thank Professor Aaron Zorn and Scott Rankin for providing Xsox18 plasmid.Peer reviewedPublisher PD

On the calculation of normals in free-surface flow problems

The use of boundary-conforming finite element methods is considered for the solution of surface-tension-dominated free-surface flow problems in three dimensions. This class of method is based upon the use of a moving mesh whose velocity is driven by the motion of the free surface, which is in turn determined via a kinematic boundary condition for the normal velocity. The significance of the method used to compute the normal direction at the finite element node points for a C0 piecewise-polynomial free surface is investigated. In particular, it is demonstrated that the concept of mass-consistent normals on an isoparametric quadratic tetrahedral mesh is flawed. In this case an alternative, purely geometric, normal is shown to lead to a far more robust numerical algorithm

S-wave superconductivity near a surface

We study the superconducting order parameter near a surface with the Bogoliubov-de Gennes formalism. For definiteness we use the attractive Hubbard model. Near a surface, the order parameter and the density distribution exhibit ``Friedel-like'' oscillations. Although the local density of states is quite different from that in the bulk, the energy gap in the spectrum on a surface is almost the same as the bulk value. In the low-density limit, however, the energy gap tends to vanish on a surface.Comment: 9 pages, 13 figure

The Shrinking Political Space of Minority Nationalist Parties in an Enlarged Europe of the Regions

From the late 1980s, European integration has been seen to have benefits for minority nationalist parties in terms of setting the political agenda, reinforcing the role of regional governments and providing second-order electoral opportunities at European elections. However, recent EU enlargements have produced a negative environment for minority nationalist parties, evident in the loss of support and MEPs in the 2004 European election. This article examines the development of minority nationalist parties at the European level since 1979, and evaluates different potential explanations for the demise of the party family since 2004

Reliability and validity of a domain-specific last 7-d sedentary time questionnaire

Purpose: The objective of this study is to examine test-retest reliability, criterion validity, and absolute agreement of a self-report, last 7-d sedentary behavior questionnaire (SIT-Q-7d), which assesses total daily sedentary time as an aggregate of sitting/lying down in five domains (meals, transportation, occupation, nonoccupational screen time, and other sedentary time). Dutch (DQ) and English (EQ) versions of the questionnaire were examined. Methods: Fifty-one Flemish adults (ages 39.4 +/- 11.1 yr) wore a thigh accelerometer (activPAL3 (TM)) and simultaneously kept a domain log for 7 d. The DQ was subsequently completed twice (median test-retest interval: 3.3 wk). Thigh-acceleration sedentary time was log annotated to create comparable domain-specific and total sedentary time variables. Four hundred two English adults (ages 49.6 +/- 7.3 yr) wore a combined accelerometer and HR monitor (Actiheart (R)) for 6 d to objectively measure total sedentary time. The EQ was subsequently completed twice (median test-retest interval: 3.4 wk). In both samples, the questionnaire reference frame overlapped with the criterion measure administration period. All participants had five or more valid days of criterion data, including one or more weekend day. Results: Test-retest reliability (intraclass correlation coefficient (95% CI)) was fair to good for total sedentary time (DQ: 0.68 (0.50-0.81); EQ: 0.53 (0.44-0.62)) and poor to excellent for domain-specific sedentary time (DQ: from 0.36 (0.10-0.57) (meals) to 0.66 (0.46-0.79) (occupation); EQ: from 0.45 (0.35-0.54) (other sedentary time) to 0.76 (0.71-0.81) (meals)). For criterion validity (Spearman rho), significant correlations were found for total sedentary time (DQ: 0.52; EQ: 0.22; all P <0.001). Compared with domain-specific criterion variables (DQ), modest-to-strong correlations were found for domain-specific sedentary time (from 0.21 (meals) to 0.76 (P < 0.001) (screen time)). The questionnaire generally overestimated sedentary time compared with criterion measures. Conclusion: The SIT-Q-7d appears to be a useful tool for ranking individuals in large-scale observational studies examining total and domain-specific sitting

Impact of wild-type and genetically modified Pseudomonas fluorescens on soil enzyme activities and microbial population structure in the rhizosphere of pea

The definitive version is available at www.blackwell-synergy.com. Copyright Blackwell Publishing DOI : 10.1046/j.1365-294x.1998.00367.xThe aim of this work was to determine the impact of wild type along with functionally and non-functionally modified Pseudomonas fluorescens strains in the rhizosphere. The wild type F113 strain carried a gene encoding the production of the antibiotic 2,4 diacetylphloroglucinol (DAPG) useful in plant disease control, and was marked with a lacZY gene cassette. The first modified strain was a functional modification of strain F113 with repressed production of DAPG, creating the DAPG negative strain F113 G22. The second paired comparison was a non-functional modification of wild type (unmarked) strain SBW25, constructed to carry marker genes only, creating strain SBW25 EeZY-6KX. Significant perturbations were found in the indigenous bacterial population structure, with the F113, (DAPG+) strain causing a shift towards slower growing colonies (K strategists) compared with the non-antibiotic producing derivative (F113 G22) and the SBW25 strains. The DAPG+ strain also significantly reduced, in comparison with the other inocula, the total Pseudomonas populations but did not affect the total microbial populations. The survival of F113 and F113 G22 were an order of magnitude lower than the SBW 25 strains. The DAPG+ strain caused a significant decrease in the shoot to root ratio in comparison to the control and other inoculants, indicating plant stress. F113 increased soil alkaline phosphatase, phosphodiesterase and aryl sulphatase activities compared to the other inocula, which themselves reduced the same enzyme activities compared to the control. In contrast to this, the -glucosidase, -galactosidase and N-acetyl glucosaminidase activities decreased with the inoculation of the DAPG+ strain. These results indicate that soil enzymes are sensitive to the impact of GMM inoculation.Peer reviewe

Monte Carlo Simulations of Sexual Reproduction

Modifying the Redfield model of sexual reproduction and the Penna model of biological aging, we compare reproduction with and without recombination in age-structured populations. In contrast to Redfield and in agreement with Bernardes we find sexual reproduction to be preferred to asexual one. In particular, the presence of old but still reproducing males helps the survival of younger females beyond their reproductive age.Comment: 8 pages, plain tex, 7 EPS figures, to appear in PHYSICA

Extending the theory of Owicki and Gries with a logic of progress

This paper describes a logic of progress for concurrent programs. The logic is based on that of UNITY, molded to fit a sequential programming model. Integration of the two is achieved by using auxiliary variables in a systematic way that incorporates program counters into the program text. The rules for progress in UNITY are then modified to suit this new system. This modification is however subtle enough to allow the theory of Owicki and Gries to be used without change

Variations in teaching of removable partial dentures in Spanish dental schools

Objectives: Despite the increasing demand for implant-based treatments, removable partial dentures (RPDs) are frequently used in the oral rehabilitation of partially dentate patients. Furthermore, the Bologna Declaration Agreement (1999) promotes the freedom of movement both of students among dental schools and of graduates across the European Union (EU). Given that inconsistency in teaching among dental schools may lead to confusion, this study aimed to evaluate the status of teaching and clinical experience reached by undergrad uate dentalstudents in Spanish dental schools in relation to RPDs. Study design: A questionnaire seeking information on the preclinical and clinical teaching of RPDs was emailed to all Spanish dental schools (11 public, 4 private) with complete undergraduate degree dental programmes in November 2009. Descriptive statistical data analysis was performed. Results: A 100% response rate was obtained. The average duration of the preclinical course in Spain was 44 hours (38 hours in the public chools and 60.5 hours in the private schools). However, public schools reported a greater number of RPDs made per student prior to graduation, with an average of 3.4 acrylic (range: 1-20) and 3.4 cobaltchromium (range: 1-20) RPDs. The corresponding means for private schools were 1 acrylic (range: 0-2) and 2.3 cobalt-chromium (range: 1-4). One public school (9%) stated that they were teaching RPDs using Problem-Based- Learning.Conclusions: Similar to that noted in previously surveyed countries, variations in teaching programmes and clinical experience concerning RPDs achieved by Spanish dental students were evident. While diversity of teaching is often considered to be of benefit, dental students must be adequately trained to ensure that they meet the needs of the patients they will serve during their careers. © Medicina Oral S. L