Carbon isotope ratios of Sterkfontein fossils indicate a marked shift to open environments c.1.7 Myr ago

Sterkfontein straddles a period in the Plio-Pleistocene in which the effects of global-scale climate shifts on continental environments and biota are not yet well understood. Reconstructions based on faunal indicators have suggested incremental shifts from relatively closed, mesic landscapes to open, arid environments at this time. We examined environmental shifts from ~2.5 to 1.7 million years ago (Myr) using a C3 / C4 index derived from 13C / 12C data on bovid fossils from Sterkfontein. Data for Member 4 and the Member 5 East infill indicate persistence of a wooded to moderately wooded environment until about 2.0-1.8 Myr. Data indicating a marked shift to open environments are observed only for the younger Member 5 West infill. We conclude that the major shift to open environments occurred near 1.7 Myr rather than at c. 2.5 Myr

The social psychology of citizenship: engagement with citizenship studies and future research

In this article we review the argument outlined in the opening article in this special thematic section: that the current social psychology of citizenship can be understood as the development of longstanding conceptualisations of the concept within the discipline. These conceptualisations have contributed to the current social psychological study of the constructive, active and collective (but often exclusive) understandings of citizenship in people’s everyday lives, as evidenced by contributions to this thematic section. We consider how this emerging body of work might fit with current citizenship studies and in particular how it may contribute to the current trend towards conceiving citizenship as an active practice embedded in everyday social life. Specifically, we highlight three areas of future research that we think are particularly promising: citizenship and recognition; displays and enactments of citizenship in public space; citizenship and lived coexistence. Although this is far from an exhaustive list of possibilities, we propose that research in these areas could enable the way for social psychology to articulate a distinct, recognisable and valuable contribution to citizenship studies

Scaling Observation Error for Optimal Assimilation of CCI SST Data into a Regional HYCOM EnOI System

South Africa currently possesses no operational ocean forecasting system for the purpose of predicting ocean state variables including temperature,salinity and velocity. Substantial initial efforts towards this goal have been made and resulted in a system using a regional Hybrid Coordinate Ocean Model (HYCOM) along with the Ensemble Optimal Interpolation (EnOI)assimilation scheme. Assimilating only sea surface temperature (SST) observations from the Operational Sea Surface Temperature and Sea Ice Analysis (OSTIA) product into the system resulted in a degraded forecast. Aiming to address this, Climate Change Initiative (CCI) SSTs are assimilated into the system in an effort to improve the forecast skill. Observation errors in the assimilated product are used in the EnOI to determine whether more confidence should be placed in the model or observations in producing the analysis, but overconfidence in observations can shock the model and result in failure. To tweak the impact of the assimilation, a scaling factor is applied in the assimilation code. A scaling factor of 25 was found to produce a favourable result with lowest mean root mean square error (RMSE;1.098C) between the model and observations over time. Postulating the error to be overconfident, a floor value is introduced in order to set a minimum value for the observation error thereby reducing confidence in the observations. These experiments fared less favourably with a floor value of 0.5 and a scaling factor of 15 producing the best mean RMSE (1.118C)

Serum procalcitonin for the early recognition of nosocomial infection in the critically ill patients: a preliminary report

<p>Abstract</p> <p>Background</p> <p>The usefulness of procalcitonin (PCT) measurement in critically ill medical patients with suspected nosocomial infection is unclear. The aim of the study was to assess PCT value for the early diagnosis of bacterial nosocomial infection in selected critically ill patients.</p> <p>Methods</p> <p>An observational cohort study in a 15-bed intensive care unit was performed. Seventy patients with either proven (n = 47) or clinically suspected but not confirmed (n = 23) nosocomial infection were included. Procalcitonin measurements were obtained the day when the infection was suspected (D0) and at least one time within the 3 previous days (D-3 to D0). Patients with proven infection were compared to those without. The diagnostic value of PCT on D0 was determined through the construction of the corresponding receiver operating characteristic (ROC) curve. In addition, the predictive value of PCT variations preceding the clinical suspicion of infection was assessed.</p> <p>Results</p> <p>PCT on D0 was the best predictor of proven infection in this population of ICU patients with a clinical suspicion of infection (AUROCC = 0.80; 95% CI, 0.68–0.91). Thus, a cut-off value of 0.44 ng/mL provides sensitivity and specificity of 65.2% and 83.0%, respectively. Procalcitonin variation between D-1 and D0 was calculated in 45 patients and was also found to be predictive of nosocomial infection (AUROCC = 0.89; 95% CI, 0.79–0.98) with a 100% positive predictive value if the +0.26 ng/mL threshold value was applied. Comparable results were obtained when PCT variation between D-2 and D0, or D-3 and D0 were considered. In contrast, CRP elevation, leukocyte count and fever had a poor predictive value in our population.</p> <p>Conclusion</p> <p>PCT monitoring could be helpful in the early diagnosis of nosocomial infection in the ICU. Both absolute values and variations should be considered and evaluated in further studies.</p

Dietary elimination of children with food protein induced gastrointestinal allergy – micronutrient adequacy with and without a hypoallergenic formula?

Background: The cornerstone for management of Food protein-induced gastrointestinal allergy (FPGIA) is dietary exclusion; however the micronutrient intake of this population has been poorly studied. We set out to determine the dietary intake of children on an elimination diet for this food allergy and hypothesised that the type of elimination diet and the presence of a hypoallergenic formula (HF) significantly impacts on micronutrient intake. Method: A prospective observational study was conducted on children diagnosed with FPIGA on an exclusion diet who completed a 3 day semi-quantitative food diary 4 weeks after commencing the diet. Nutritional intake where HF was used was compared to those without HF, with or without a vitamin and mineral supplement (VMS). Results: One-hundred-and-five food diaries were included in the data analysis: 70 boys (66.7%) with median age of 21.8 months [IQR: 10 - 67.7]. Fifty-three children (50.5%) consumed a HF and the volume of consumption was correlated to micronutrient intake. Significantly (p <0.05) more children reached their micronutrient requirements if a HF was consumed. In those without a HF, some continued not to achieve requirements in particular for vitamin D and zinc, in spite of VMS. Conclusion: This study points towards the important micronutrient contribution of a HF in children with FPIGA. Children, who are not on a HF and without a VMS, are at increased risk of low intakes in particular vitamin D and zinc. Further studies need to be performed, to assess whether dietary intake translates into actual biological deficiencies

A framework for mixing methods in quantitative measurement development, validation, and revision: A case study

A framework for quantitative measurement development, validation, and revision that incorporates both qualitative and quantitative methods is introduced. It extends and adapts Adcock and Collier’s work, and thus, facilitates understanding of quantitative measurement development, validation, and revision as an integrated and cyclical set of procedures best achieved through mixed methods research. It also offers a systematic guide concerning how these procedures may be undertaken through detailing key “stages,” “levels,” and practical “tasks.” A case study illustrates how qualitative and quantitative methods may be mixed through the use of the proposed framework in the cross-cultural content- and construct-related validation and subsequent revision of a quantitative measure.The contribution of this article to mixed methods research literature is briefly discussed

Variants of the EAAT2 Glutamate Transporter Gene Promoter Are Associated with Cerebral Palsy in Preterm Infants

© 2017, The Author(s). Preterm delivery is associated with neurodevelopmental impairment caused by environmental and genetic factors. Dysfunction of the excitatory amino acid transporter 2 (EAAT2) and the resultant impaired glutamate uptake can lead to neurological disorders. In this study, we investigated the role of single nucleotide polymorphisms (SNPs; g.-200CCloseSPigtSPiA and g.-181ACloseSPigtSPiC) in the EAAT2 promoter in susceptibility to brain injury and neurodisability in very preterm infants born at or before 32-week gestation. DNA isolated from newborns’ dried blood spots were used for pyrosequencing to detect both SNPs. Association between EAAT2 genotypes and cerebral palsy, cystic periventricular leukomalacia and a low developmental score was then assessed. The two SNPs were concordant in 89.4% of infants resulting in three common genotypes all carrying two C and two A alleles in different combinations. However, in 10.6% of cases, non-concordance was found, generating six additional rare genotypes. The A alleles at both loci appeared to be detrimental and consequently, the risk of developing cerebral palsy increased four- and sixfold for each additional detrimental allele at -200 and -181bp, respectively. The two SNPs altered the regulation of the EAAT2 promoter activity and glutamate homeostasis. This study highlights the significance of glutamate in the pathogenesis of preterm brain injury and subsequent development of cerebral palsy and neurodevelopmental disabilities. Furthermore, the described EAAT2 SNPs may be an early biomarker of vulnerability to neurodisability and may aid the development of targeted treatment strategies

Surveillance of multiple congenital anomalies; searching for new associations

\ua9 2023, The Author(s).Many human teratogens are associated with a spectrum of congenital anomalies rather than a single defect, and therefore the identification of congenital anomalies occurring together more frequently than expected may improve the detection of teratogens. Thirty-two EUROCAT congenital anomaly registries covering 6,599,765 births provided 123,566 cases with one or more major congenital anomalies (excluding chromosomal and genetic syndromes) for the birth years 2008–2016. The EUROCAT multiple congenital anomaly algorithm identified 8804 cases with two or more major congenital anomalies in different organ systems, that were not recognized as part of a syndrome or sequence. For each pair of anomalies, the odds of a case having both anomalies relative to having only one anomaly was calculated and the p value was estimated using a two-sided Fisher’s exact test. The Benjamini–Hochberg procedure adjusted p values to control the false discovery rate and pairs of anomalies with adjusted p values &lt; 0.05 were identified. A total of 1386 combinations of two anomalies were analyzed. Out of the 31 statistically significant positive associations identified, 20 were found to be known associations or sequences already described in the literature and 11 were considered “potential new associations” by the EUROCAT Coding and Classification Committee. After a review of the literature and a detailed examination of the individual cases with the anomaly pairs, six pairs remained classified as new associations. In summary, systematically searching for congenital anomalies occurring together more frequently than expected using the EUROCAT database is worthwhile and has identified six new associations that merit further investigation