Stable isotope labeling by amino acids in cell cultur (SILAC) as a tool in cancer research

Comunicaciones a congreso

Vitamin C activates young LINE-1 elements in mouse embryonic stem cells via H3K9me3 demethylation

This work was supported by grants from the Wellcome Trust/Royal Soci‑ ety (101225/Z/13/Z) and MRC (MR/X008487/1) to M.R.B.; and BBSRC (BB/ T000031/1) to M.R.B. and J.M.F.Background Vitamin C (vitC) enhances the activity of 2-oxoglutarate-dependent dioxygenases, including TET enzymes, which catalyse DNA demethylation, and Jumonji-domain histone demethylases. The epigenetic remodelling promoted by vitC improves the efficiency of induced pluripotent stem cell derivation, and is required to attain a ground-state of pluripotency in embryonic stem cells (ESCs) that closely mimics the inner cell mass of the early blastocyst. However, genome-wide DNA and histone demethylation can lead to upregulation of transposable elements (TEs), and it is not known how vitC addition in culture media affects TE expression in pluripotent stem cells. Results Here we show that vitC increases the expression of several TE families, including evolutionarily young LINE-1 (L1) elements, in mouse ESCs. We find that TET activity is dispensable for L1 upregulation, and that instead it occurs largely as a result of H3K9me3 loss mediated by KDM4A/C histone demethylases. Despite increased L1 levels, we did not detect increased somatic insertion rates in vitC-treated cells. Notably, treatment of human ESCs with vitC also increases L1 protein levels, albeit through a distinct, post-transcriptional mechanism. Conclusion VitC directly modulates the expression of mouse L1s and other TEs through epigenetic mechanisms, with potential for downstream effects related to the multiple emerging roles of L1s in cellular function.Wellcome Trust/Royal Society (101225/Z/13/Z)MRC (MR/X008487/1)BBSRC (BB/T000031/1

Education as the creation of microcultures. From the local community to the virtual network

This article presents the work undertaken by a multidisciplinary group, comprising researchers from the fields of psychology, education and technology, as we set about developing new virtual tools for collaborative learning. We began from our experience acquired in learning communities based on the "Fifth Dimension" model, in which ICTs are used in a collaborative fashion. The work undertaken with members of ethnic minority groups in communities of this type underlies the theoretical and practical development of the foundations for intercultural education. Finally, we describe the process by which we constructed virtual tools that can be appropriated from a wide variety of contexts

A Multi-Omics Approach to Evaluate the Toxicity Mechanisms Associated with Silver Nanoparticles Exposure

Silver nanoparticles (AgNPs) are currently used in many different industrial, commercial and health fields, mainly due to their antibacterial properties. Due to this widespread use, humans and the environment are increasingly exposed to these types of nanoparticles, which is the reason why the evaluation of the potential toxicity associated with AgNPs is of great importance. Although some of the toxic effects induced by AgNPs have already been shown, the elucidation of more complete mechanisms is yet to be achieved. In this sense, and since the integration of metabolomics and transcriptomics approaches constitutes a very useful strategy, in the present study targeted and untargeted metabolomics and DNA microarrays assays have been combined to evaluate the molecular mechanisms involved in the toxicity induced by 10 nm AgNPs. The results have shown that AgNPs induce the synthesis of glutathione as a cellular defense mechanism to face the oxidative environment, while inducing the depletion of relevant molecules implicated in the synthesis of important antioxidants. In addition, it has been observed that AgNPs completely impair the intracellular energetic metabolism, especially affecting the production of adenosine triphosphate (ATP) and disrupting the tricarboxylic acids cycle. It has been demonstrated that AgNPs exposure also affects the glycolysis pathway. The effect on such pathway differs depending on the step of the cycle, which a significant increase in the levels of glucose as way to counterbalance the depleted levels of ATP.Depto. de Química AnalíticaFac. de Ciencias QuímicasTRUEMinisterio de Ciencia, Innovaciónpu

L'educació com a creació de microcultures : de la comunitat local a la xarxa virtual

Este artículo presenta el trabajo llevado a cabo por un grupo multidisciplinario que comprende investigadores del campo de la psicología, la educación y la tecnología, dado que se quieren desarrollar nuevas herramientas virtuales para el aprendizaje colaborativo. Se comenzó desde la experiencia adquirida en comunidades de aprendizaje basadas en el modelo Cinquena Dimensió donde las TIC son utilizadas de manera colaborativa. A través del trabajo con miembros de grupos étnicos minoritarios en comunidades de este tipo se persigue el desarrollo teórico y práctico de los fundamentos de la educación intercultural. Finalmente, se describe el proceso mediante el cual se construyeron herramientas virtuales que pueden ser apropiadas desde una amplia variedad de contextosCet article présente le travail mené à terme par un groupe multidisciplinaire qui comprend des chercheurs des domaines de la psychologie, de l'éducation et de la technologie, afi n de développer de nouveaux outils virtuels pour l'apprentissage en collaboration. Le travail a été entamé à partir de l'expérience acquise dans des communautés d'apprentissage basées sur le modèle de la «cinquième dimension » dans lequel les TIC sont utilisées comme collaboratrices. C'est grâce au travail avec des membres de groupes ethniques minoritaires dans des communautés de ce type que se poursuit le développement théorique et pratique des fondements de l'éducation interculturelle. Enfin, l'étude décrit le processus grâce auquel ont été construits des outils virtuels pouvant être appropriés dans une grande variété de contextes.This paper presents the study carried out by a multi-disciplinary group encompassing psychology, education and technology field researchers with the aim of developing new virtual tools for collaborative learning. Work began through the experience acquired in learning communities based on the "Fifth Dimension" model, where ICTs are used collaboratively. Through work with members of minority ethnic groups in communities of this type, the theoretical and practical development of the foundations of intercultural education is pursued. Last of all, there is a description of the process through which virtual tools were constructed that may be appropriate in a wide range of contexts

Effect of Bay Leaves Essential Oil Concentration on the Properties of Biodegradable Carboxymethyl Cellulose-Based Edible Films

Films containing bay leaves essential oils (BEOs) were prepared and evaluated for edible packaging applications. The BEOs were extracted by the Soxhlet method, using ethanol or methanol as organic solvent. Then, films were prepared by “solvent casting” technique using carboxymethyl cellulose (CMC), with different concentrations for the as-obtained BEOs (from 1% to 30% wt.). The resulting films were characterized to evaluate their physical (thickness, moisture content, water solubility and water vapor permeability), optical (transparency and UV-light barrier), mechanical (tensile strength and elongation at break), antioxidant and antimicrobiological properties Attractive films were obtained for food active packaging applications, as they presented a high antioxidant activity (up to 99%) and total phenolic content, and good barrier properties against water vapor (50% improved of CMC) in the case of CMC-film containing 15% wt. ethanolic extract. Related to optical properties, UV-light barrier effect was increased (almost 100% of protection) avoiding typical lipids oxidation in food systems. High water solubility (93%) was also found, ensuring also their biodegradability. Moreover, it was demonstrated that developed films inhibit microorganisms’ growth (Escherichia coli and Candida glabrata), this avoiding an early food oxidation

Dolor abdominal y parada cardíaca. Una presentación inusual de hematoma de los rectos

Spontaneous hematoma of the rectus abdominis sheath is a cause of infrequent abdominal pain. Most common form of presentation is abdominal pain of sudden onset, followed by the finding of an abdominal mass. In the case of large bruises these can cause symptoms and signs derived from hypovolemia. Anticoagulant therapy is considered the most frequent risk factor, especially if the patients are elderly women whose onset of symptoms appeared after a coughing attack. We present a case of a spontaneous straight hematoma sheath presenting as a sudden cardiac arrest that ultimately caused the death of the patient.El hematoma espontáneo de la vaina del recto abdominal es una causa de dolor abdominal infrecuente. Su forma más común de presentación es dolor abdominal de inicio súbito, seguido del hallazgo de una masa abdominal. En el caso de hematomas grandes, éstos pueden causar síntomas y signos derivados de la hipovolemia. La terapia anticoagulante se considera el factor de riesgo más frecuente, especialmente si los pacientes son mujeres de edad avanzada y los síntomas aparecieron después de un ataque de tos. Presentamos un caso de hematoma espontáneo de la vaina del recto cuya presentación fue un paro cardíaco repentino que, finalmente, causó la muerte del paciente

Un espai de col·laboració entre la comunitat gitana i la Universitat per a l'educació intercultural

Se presenta el proyecto pedagógico que se lleva a cabo en la Casa de Shere Rom, creada en el sí de la Asociación Gitana de Badalona (Barcelona) en la cual miembros de la misma comunidad participan como educadores conjuntamente, y en relación de igualdad, con estudiantes universitarios en un espacio intercultural. Este proyecto nace con el objetivo de disminuir el elevado índice de fracaso escolar de la comunidad gitana y utiliza un modelo de aprendizaje colaborativo, a la vez que se integran las Tecnologías de la Información y la Comunicación (TIC