2,553 research outputs found
Analysis on Aging in the Generalized Random Energy Model
A new dynamics more natural than that proposed by Bouchaud and Dean is
introduced to the Generalized Random Energy Model, and the master equation for
the dynamics is solved exactly to calculate the time correlation function.
Although our results are very similar to those obtained by Bouchaud and Dean
qualitatively, the exponents for power law relaxation are different. The
Zero-Field-Cooled magnetization is also calculated with a relation between the
correlation function and the response function which holds even if the
relaxation is non-equilibrium. The validity of these analytic results are
confirmed by numerical simulations.Comment: 12 pages, 5 figures, submitted to J. Phys. Sci. Jp
Transcript expression of vesicular glutamate transporters in lumbar dorsal root ganglia and the spinal cord of mice – Effects of peripheral axotomy or hindpaw inflammation
Using specific riboprobes, we characterized the expression of vesicular glutamate transporter (VGLUT)1–VGLUT3 transcripts in lumbar 4–5 (L4–5) dorsal root ganglions (DRGs) and the thoracolumbar to lumbosacral spinal cord in male BALB/c mice after a 1- or 3-day hindpaw inflammation, or a 7-day sciatic nerve axotomy. Sham animals were also included. In sham and contralateral L4–5 DRGs of injured mice, VGLUT1-, VGLUT2- and VGLUT3 mRNAs were expressed in ∼45%, ∼69% or ∼17% of neuron profiles (NPs), respectively. VGLUT1 was expressed in large and medium-sized NPs, VGLUT2 in NPs of all sizes, and VGLUT3 in small and medium-sized NPs. In the spinal cord, VGLUT1 was restricted to a number of NPs at thoracolumbar and lumbar segments, in what appears to be the dorsal nucleus of Clarke, and in mid laminae III–IV. In contrast, VGLUT2 was present in numerous NPs at all analyzed spinal segments, except the lateral aspects of the ventral horns, especially at the lumbar enlargement, where it was virtually absent. VGLUT3 was detected in a discrete number of NPs in laminae III–IV of the dorsal horn. Axotomy resulted in a moderate decrease in the number of DRG NPs expressing VGLUT3, whereas VGLUT1 and VGLUT2 were unaffected. Likewise, the percentage of NPs expressing VGLUT transcripts remained unaltered after hindpaw inflammation, both in DRGs and the spinal cord. Altogether, these results confirm previous descriptions on VGLUTs expression in adult mice DRGs, with the exception of VGLUT1, whose protein expression was detected in a lower percentage of mouse DRG NPs. A detailed account on the location of neurons expressing VGLUTs transcripts in the adult mouse spinal cord is also presented. Finally, the lack of change in the number of neurons expressing VGLUT1 and VGLUT2 transcripts after axotomy, as compared to data on protein expression, suggests translational rather than transcriptional regulation of VGLUTs after injury.Fil: Malet, Mariana. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Vieytes, C. A.. Universidad Austral. Facultad de Ciencias Biomédicas; ArgentinaFil: Lundgren, K. H.. University of Cincinnati; Estados UnidosFil: Seal, R. P.. University of Pittsburgh; Estados UnidosFil: Tomasella, MarÃa Eugenia. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Seroogy, K. B.. University of Cincinnati; Estados UnidosFil: Hökfelt, T.. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Gebhart, G. F.. University of Pittsburgh; Estados UnidosFil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina. University of Pittsburgh; Estados Unido
Spatially Extended Low Ionization Emission Regions (LIERs) at
We present spatially resolved emission diagnostics for eight
galaxies that demonstrate extended low ionization emission-line regions (LIERs)
over kpc scales. Eight candidates are selected based on their spatial extent
and emission line fluxes from slitless spectroscopic observations with the
HST/WFC3 G141 and G800L grisms in the well-studied GOODS survey fields. Five of
the candidates (62.5%) are matched to X-ray counterparts in the \textit{Chandra
X-Ray Observatory} Deep Fields. We modify the traditional
Baldwin-Philips-Terlevich (BPT) emission line diagnostic diagram to use
[SII]/(H+[NII]) instead of [NII]/H to overcome the blending of
[NII] and H+[NII] in the low resolution slitless grism spectra. We
construct emission line ratio maps and place the individual pixels in the
modified BPT. The extended LINER-like emission present in all of our
candidates, coupled with X-Ray properties consistent with star-forming galaxies
and weak [OIII]5007\AA\ detections, is inconsistent with purely
nuclear sources (LINERs) driven by active galactic nuclei. While recent
ground-based integral field unit spectroscopic surveys have revealed
significant evidence for diffuse LINER-like emission in galaxies within the
local universe , this work provides the first evidence for the
non-AGN origin of LINER-like emission out to high redshifts.Comment: 11 pages, 1 table, 6 figures, accepted for publication in the
Astrophysics Journal (ApJ
The Number Density Evolution of Extreme Emission Line Galaxies in 3D-HST: Results from a Novel Automated Line Search Technique for Slitless Spectroscopy
The multiplexing capability of slitless spectroscopy is a powerful asset in
creating large spectroscopic datasets, but issues such as spectral confusion
make the interpretation of the data challenging. Here we present a new method
to search for emission lines in the slitless spectroscopic data from the 3D-HST
survey utilizing the Wide-Field Camera 3 on board the Hubble Space Telescope.
Using a novel statistical technique, we can detect compact (extended) emission
lines at 90% completeness down to fluxes of 1.5 (3.0) times 10^{-17}
erg/s/cm^2, close to the noise level of the grism exposures, for objects
detected in the deep ancillary photometric data. Unlike previous methods, the
Bayesian nature allows for probabilistic line identifications, namely redshift
estimates, based on secondary emission line detections and/or photometric
redshift priors. As a first application, we measure the comoving number density
of Extreme Emission Line Galaxies (restframe [O III] 5007 equivalent widths in
excess of 500 Angstroms). We find that these galaxies are nearly 10 times more
common above z~1.5 than at z<0.5. With upcoming large grism surveys such as
Euclid and WFIRST as well as grisms featuring prominently on the NIRISS and
NIRCam instruments on James Webb Space Telescope, methods like the one
presented here will be crucial for constructing emission line redshift catalogs
in an automated and well-understood manner.Comment: 16 pages, 14 Figures; Accepted to Ap
Expression of vesicular glutamate transporters in sensory and autonomic neurons innervating the mouse urinary bladder
Purpose: Vesicular glutamate transporters (VGLUTs), essential for loading glutamate into synaptic vesicles, are present in various neuronal systems. However, the expression of VGLUTs in neurons innervating the urinary bladder has not yet been analyzed. Here, we study the presence of VGLUTs type-1, -2 and -3 (VGLUT1, VGLUT2 and VGLUT3, respectively) in mouse urinary bladder neurons. Materials and Methods: Expression of VGLUT1, VGLUT2 and calcitonin gene-related peptide (CGRP) was analyzed by immunohistochemistry in retrogradely labeled primary afferent and autonomic neurons of BALB/C mice after injecting Fast Blue in the urinary bladder wall. To study VGLUT3, retrograde tracing of the urinary bladder was performed in transgenic mice where VGLUT3 is identified by detection of enhanced green fluorescent protein (EGFP). Results: Most urinary bladder DRG neurons expressed VGLUT2. A smaller percentage of neurons also expressed VGLUT1 or VGLUT3. Coexpression with CGRP was only observed for VGLUT2. Occasional VGLUT2-immunoreactive (IR) neurons were seen in the major pelvic ganglion (MPG). Abundant VGLUT2-IR nerves were detected in the urinary bladder dome, trigone and also the urethra; VGLUT1-IR nerves were discretely present. Conclusions: We present novel data on the expression of VGLUTs in sensory and autonomic neurons innervating the mouse urinary bladder. The frequent association of VGLUT2 and CGRP in sensory neurons suggests interactions between glutamatergic and peptidergic neurotransmissions, potentially influencing commonly perceived sensations in the urinary bladder, such as discomfort and pain.Fil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas. Laboratorio de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina. University of Pittsburgh. Department of Anesthesiology. Pittsburgh Center for Pain Research; Estados UnidosFil: Seal, Rebecca P.. University of Pittsburgh. Department of Anesthesiology. Pittsburgh Center for Pain Research; Estados UnidosFil: Lundgren, Kerstin H.. University of Cincinnati. Department of Neurology; Estados UnidosFil: Seroogy, Kim B.. University of Cincinnati. Department of Neurology; Estados UnidosFil: Watanabe, Masahiko. Hokkaido University School of Medicine. Department of Anatomy; JapónFil: Gebhart, G. F.. University of Pittsburgh. Department of Anesthesiology. Pittsburgh Center for Pain Research; Estados Unido
Memory Effect, Rejuvenation and Chaos Effect in the Multi-layer Random Energy Model
We introduce magnetization to the Multi-layer Random Energy Model which has a
hierarchical structure, and perform Monte Carlo simulation to observe the
behavior of ac-susceptibility. We find that this model is able to reproduce
three prominent features of spin glasses, i.e., memory effect, rejuvenation and
chaos effect, which were found recently by various experiments on aging
phenomena with temperature variations.Comment: 10 pages, 14 figures, to be submitted to J. Phys. Soc. Jp
Numerical Study of Aging in the Generalized Random Energy Model
Magnetizations are introduced to the Generalized Random Energy Model (GREM)
and numerical simulations on ac susceptibility is made for direct comparison
with experiments in glassy materials. Prominent dynamical natures of spin
glasses, {\it i.e.}, {\em memory} effect and {\em reinitialization}, are
reproduced well in the GREM. The existence of many layers causing continuous
transitions is very important for the two natures. Results of experiments in
other glassy materials such as polymers, supercooled glycerol and orientational
glasses, which are contrast to those in spin glasses, are interpreted well by
the Single-layer Random Energy Model.Comment: 8 pages, 9 figures, to be submitted to J. Phys. Soc. Jp
Interleukin-2 therapy in patients with HIV infection
BACKGROUND
Used in combination with antiretroviral therapy, subcutaneous recombinant interleukin-2 raises CD4+ cell counts more than does antiretroviral therapy alone. The clinical implication of these increases is not known.
METHODS
We conducted two trials: the Subcutaneous Recombinant, Human Interleukin-2 in HIV-Infected Patients with Low CD4+ Counts under Active Antiretroviral Therapy (SILCAAT) study and the Evaluation of Subcutaneous Proleukin in a Randomized International Trial (ESPRIT). In each, patients infected with the human immunodeficiency virus (HIV) who had CD4+ cell counts of either 50 to 299 per cubic millimeter (SILCAAT) or 300 or more per cubic millimeter (ESPRIT) were randomly assigned to receive interleukin-2 plus antiretroviral therapy or antiretroviral therapy alone. The interleukin-2 regimen consisted of cycles of 5 consecutive days each, administered at 8-week intervals. The SILCAAT study involved six cycles and a dose of 4.5 million IU of interleukin-2 twice daily; ESPRIT involved three cycles and a dose of 7.5 million IU twice daily. Additional cycles were recommended to maintain the CD4+ cell count above predefined target levels. The primary end point of both studies was opportunistic disease or death from any cause.
RESULTS
In the SILCAAT study, 1695 patients (849 receiving interleukin-2 plus antiretroviral therapy and 846 receiving antiretroviral therapy alone) who had a median CD4+ cell count of 202 cells per cubic millimeter were enrolled; in ESPRIT, 4111 patients (2071 receiving interleukin-2 plus antiretroviral therapy and 2040 receiving antiretroviral therapy alone) who had a median CD4+ cell count of 457 cells per cubic millimeter were enrolled. Over a median follow-up period of 7 to 8 years, the CD4+ cell count was higher in the interleukin-2 group than in the group receiving antiretroviral therapy alone--by 53 and 159 cells per cubic millimeter, on average, in the SILCAAT study and ESPRIT, respectively. Hazard ratios for opportunistic disease or death from any cause with interleukin-2 plus antiretroviral therapy (vs. antiretroviral therapy alone) were 0.91 (95% confidence interval [CI], 0.70 to 1.18; P=0.47) in the SILCAAT study and 0.94 (95% CI, 0.75 to 1.16; P=0.55) in ESPRIT. The hazard ratios for death from any cause and for grade 4 clinical events were 1.06 (P=0.73) and 1.10 (P=0.35), respectively, in the SILCAAT study and 0.90 (P=0.42) and 1.23 (P=0.003), respectively, in ESPRIT.
CONCLUSIONS
Despite a substantial and sustained increase in the CD4+ cell count, as compared with antiretroviral therapy alone, interleukin-2 plus antiretroviral therapy yielded no clinical benefit in either study. (ClinicalTrials.gov numbers, NCT00004978 [ESPRIT] and NCT00013611 [SILCAAT study].
Transcriptomes from German shepherd dogs reveal differences in immune activity between atopic dermatitis affected and control skin
Canine atopic dermatitis (CAD) is an inflammatory and pruritic allergic skin disease with both genetic and environmental risk factors described. We performed mRNA sequencing of non-lesional axillary skin biopsies from nine German shepherd dogs. Obtained RNA sequences were mapped to the dog genome (CanFam3.1) and a high-quality skin transcriptome was generated with 23,510 expressed gene transcripts. Differentially expressed genes (DEGs) were defined by comparing three controls to five treated CAD cases. Using a leave-one-out analysis, we identified seven DEGs: five known to encode proteins with functions related to an activated immune system (CD209, CLEC4G, LOC102156842 (lipopolysaccharide-binding protein-like), LOC480601 (regakine-1-like), LOC479668 (haptoglobin-like)), one (OBP) encoding an odorant-binding protein potentially connected to rhinitis, and the last (LOC607095) encoding a novel long non-coding RNA. Furthermore, high mRNA expression of inflammatory genes was found in axillary skin from an untreated mild CAD case compared with healthy skin. In conclusion, we define genes with different expression patterns in CAD case skin helping us understand post-treatment atopic skin. Further studies in larger sample sets are warranted to confirm and to transfer these results into clinical practice
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