A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass

The conventional biochemical platform featuring enzymatic hydrolysis involves five key steps: pretreatment, cellulase production, enzymatic hydrolysis, fermentation, and product recovery. Sugars are produced as reactive intermediates for subsequent fermentation to fuels and chemicals. Herein, an alternative biochemical route is proposed. Pretreatment, enzymatic hydrolysis and cellulase production is consolidated into one single step, referred to as consolidated aerobic processing, and sugar aldonates are produced as the reactive intermediates for biofuels production by fermentation. In this study, we demonstrate the viability of consolidation of the enzymatic hydrolysis and cellulase production steps in the new route using Neurospora crassa as the model microorganism and the conversion of cellulose to ethanol as the model system. We intended to prove the two hypotheses: 1) cellulose can be directed to produce cellobionate by reducing β-glucosidase production and by enhancing cellobiose dehydrogenase production; and 2) both of the two hydrolysis products of cellobionate—glucose and gluconate—can be used as carbon sources for ethanol and other chemical production. Our results showed that knocking out multiple copies of β-glucosidase genes led to cellobionate production from cellulose, without jeopardizing the cellulose hydrolysis rate. Simulating cellobiose dehydrogenase over-expression by addition of exogenous cellobiose dehydrogenase led to more cellobionate production. Both of the two hydrolysis products of cellobionate: glucose and gluconate can be used by Escherichia coli KO 11 for efficient ethanol production. They were utilized simultaneously in glucose and gluconate co-fermentation. Gluconate was used even faster than glucose. The results support the viability of the two hypotheses that lay the foundation for the proposed new route

Analogue models of the effect of long-term basement fault movement on volcanic edifices

Long-term fault movement under volcanoes can control the edifice structure and can generate collapse events. To study faulting effects, we explore a wide range of fault geometries and motions, from normal, through vertical to reverse and dip-slip to strike-slip, using simple analogue models. We explore the effect of cumulative sub-volcanic fault motions and find that there is a strong influence on the structural evolution and potential instability of volcanoes. The variety of fault types and geometries are tested with realistically scaled displacements, demonstrating a general tendency to produce regions of instability parallel to fault strike, whatever the fault motion. Where there is oblique-slip faulting, the instability is always on the downthrown side and usually in the volcano flank sector facing the strike-slip sense of motion. Different positions of the fault beneath the volcano change the location, type and magnitude of the instability produced. For example, the further the fault is from the central axis, the larger the destabilised sector. Also, with greater fault offset from the central axis larger unstable volumes are generated. Such failures are normal to fault strike. Using simple geometric dimensionless numbers, such as the fault dip, degree of oblique motion (angle of obliquity), and the fault position, we graphically display the geometry of structures produced. The models are applied to volcanoes with known underlying faults, and we demonstrate the importance of these faults in determining volcanic structures and slope instability. Using the knowledge of fault patterns gained from these experiments, geological mapping on volcanoes can locate fault influence and unstable zones, and hence monitoring of unstable flanks could be carried out to determine the actual response to faulting in specific cases

Antarctic ice shelf disintegration triggered by sea ice loss and ocean swell

Published online 13 June 2018Understanding the causes of recent catastrophic ice shelf disintegrations is a crucial step towards improving coupled models of the Antarctic Ice Sheet and predicting its future state and contribution to sea-level rise. An overlooked climate-related causal factor is regional sea ice loss. Here we show that for the disintegration events observed (the collapse of the Larsen A and B and Wilkins ice shelves), the increased seasonal absence of a protective sea ice buffer enabled increased flexure of vulnerable outer ice shelf margins by ocean swells that probably weakened them to the point of calving. This outer-margin calving triggered wider-scale disintegration of ice shelves compromised by multiple factors in preceding years, with key prerequisites being extensive flooding and outer-margin fracturing. Wave-induced flexure is particularly effective in outermost ice shelf regions thinned by bottom crevassing. Our analysis of satellite and ocean-wave data and modelling of combined ice shelf, sea ice and wave properties highlights the need for ice sheet models to account for sea ice and ocean waves.Robert A. Massom, Theodore A. Scambos, Luke G. Bennetts, Phillip Reid, Vernon A. Squire and Sharon E. Stammerjoh