Typization and method establishment for effective isolation of IgE from serum of hypersensibilized patients

Optimalizace podmínek izolace imunoglobulinu E k následnému použití pro přípravu specifických imunoafinitních mikroreaktorů a vyhledávání alergogenních epitopů. Imunoglobulin E byl izolován metodou iontově výměnné chromatografie a afinitní chromatografie s využitím anti-IgE protilátek nebo Concanavalinu A.Katedra biologických a biochemických vědDokončená práce s úspěšnou obhajobo

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Katedra výtvarné výchovyFaculty of EducationPedagogická fakult

Variabilita obsahu a složení silic levandule lékařské z různých proveniencí

The aim of this work was the determination of variability of the essential oil content in lavender obtained from different sources. The results indicate that significant differences were found both in the essential oil content and in its composition. Only the samples of the Lavandula angustifolia P. MILL (L. officinalis CHAIX) were used to obtain the essential oils. According to the Czech Pharmacopoeia 2014, the drug (lavender flowers) should contain at least 1.3 ml of essential oil in 100 g of anhydrous drug and lavender essential oil should contain following ingredients: limonene 1.0%, lavandulol > 0.1%, α-terpineol < 2.0%. In addition, five microbial lavender samples of different provenances were determined by microbiological purity of the drug, whereby the amount of colonies per 1 ml was determined for the total number of microorganisms, spore microorganisms, enterobacteria, yeasts and fungi

Probiotické mikroorganismy a jejich význam

ABSTRACT Bachelor thesis titled Probitic microorganisms and their significance is divided into two parts, the first theoretical part deals with the characteristics of probiotic microorganisms and practical part focuses on the composition of microorganisms in fermented milk products. At the beginning of the theoretical part describes the history of the development of research probiotic microorganisms The composition of the colonization of the gastrointestinal tract and the action of microorganisms on human health. The work focuses on the description of genera probiotic microorganisms, especially on the most used genera Lactobacillus and Bifidobacterium, which are used in the dairy, meat industry and the pharmaceutical industry to produce food and pharmaceutic preparations, which aim to promote the health of people and animals. The practical part deals with the microbial composition of fermented milk products and their evaluation in terms of legislation

Sensitive enzyme immunoassay for screening methandienone in dietary supplements

<p>Methandienone is a synthetic exogenous steroid which, like other anabolic steroids, is strictly regulated in many countries. In recent years, increasing numbers have been detected of illegal additions into dietary supplements of methandienone and other anabolic androgenic steroids (AAS). In this work, a competitive indirect enzyme-linked immunosorbent assay (ELISA) has been constructed for the detection of methandienone using an antiserum against methandienone. Under optimal experimental conditions, the ELISA achieved a limit of detection of 0.04 ± 0.01 µg.g⁻¹. The obtained intra- and inter-day coefficients of variation were less than 8%. The developed ELISA was applied in the analysis of real dietary supplement samples. To minimise the effect of the sample matrix, the sample extracts were simply diluted before addition into the immunoassay. The achieved recovery values were around 100%. Results obtained from the ELISA correlated well, both in terms of accuracy and precision, with those obtained by UHPLC-MS/MS (reference method). The presented ELISA could be successfully applied for the simple screening of dietary supplements.</p

Diphyllin Shows a Broad-Spectrum Antiviral Activity against Multiple Medically Important Enveloped RNA and DNA Viruses

Diphyllin is a natural arylnaphtalide lignan extracted from tropical plants of particular importance in traditional Chinese medicine. This compound has been described as a potent inhibitor of vacuolar (H+)ATPases and hence of the endosomal acidification process that is required by numerous enveloped viruses to trigger their respective viral infection cascades after entering host cells by receptor-mediated endocytosis. Accordingly, we report here a revised, updated, and improved synthesis of diphyllin, and demonstrate its antiviral activities against a panel of enveloped viruses from Flaviviridae, Phenuiviridae, Rhabdoviridae, and Herpesviridae families. Diphyllin is not cytotoxic for Vero and BHK-21 cells up to 100 &micro;M and exerts a sub-micromolar or low-micromolar antiviral activity against tick-borne encephalitis virus, West Nile virus, Zika virus, Rift Valley fever virus, rabies virus, and herpes-simplex virus type 1. Our study shows that diphyllin is a broad-spectrum host cell-targeting antiviral agent that blocks the replication of multiple phylogenetically unrelated enveloped RNA and DNA viruses. In support of this, we also demonstrate that diphyllin is more than just a vacuolar (H+)ATPase inhibitor but may employ other antiviral mechanisms of action to inhibit the replication cycles of those viruses that do not enter host cells by endocytosis followed by low pH-dependent membrane fusion