17 research outputs found

    CHaraCTEriZaTiON OF β-TuBuliN CdNa FrOM a BENZiMidaZOlE rESiSTaNT STRONGYLOIDES STERCORALIS iSOlaTE

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    Abstract. Previous reports have indicated that benzimidazole resistance in gastrointestinal nematodes is linked to a mutation in the β-tubulin codons 167 and 200. In our study, total RNA was isolated from an albendazole-resistant (ABZR) Strongyloides stercoralis filariform larval isolate, followed by reverse transcription PCR that was amplified using primers designed according to the alignment of the β-tubulin mRNA of S. stercoralis (GenBank accession No. AY 898942). The cDNA sequence of the β-tubulin gene of ABZR S. stercoralis larvae revealed 100% identity with the sequence of S. stercoralis (AY 898942). The polymorphisms at codons 167 and 200 encoded phenylalanine (Phe). The resistance mechanisms for benzimidazole in S. stercoralis were discussed in the light of these results
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