276 research outputs found
Relatório de Estágio da Prática de Ensino Supervisionada
Deste trabalho consta a prática desenvolvida no estágio realizado de acordo com o regulamento da Prática de Ensino Supervisionada (PES) do Mestrado em Ensino do 1º e 2º Ciclo que confere habilitação profissional para a docência no 1º Ciclo e nas disciplinas de Matemática, Língua Portuguesa, Ciências da Natureza, História e Geografia de Portugal do 2º Ciclo do Ensino Básico.
O objetivo deste relatório é refletir o percurso de formação, a atitude crítica e reflexiva em relação aos desafios, processos e desempenhos do quotidiano profissional experienciado, sendo evidenciadas as dificuldades sentidas e as estratégias utilizadas para ultrapassar os obstáculos. Será, também, apresentada uma investigação sobre a inclusão que designamos de “As Atitudes dos Pares Face à Inclusão de Crianças com Necessidades Educativas Especiais”.
A inclusão de crianças com Necessidades Educativas Especiais (NEE) nas nossas escolas é um processo que, para além de educativo e pedagógico, se pretende que seja social e emocional, isto é, que conduza a um clima educativo de sucesso para todos. Os pares são neste processo um elemento chave para a criação de um ambiente inclusivo de sucesso, que une a parte educativa à parte do desenvolvimento afetivo e social. Por este motivo, este trabalho tem como principal objetivo conhecer o modo como a criança com NEE é aceite pelos seus pares numa escola inclusiva do 1º Ciclo de Sátão.
O instrumento escolhido para a recolha de dados foi o Inquérito por Questionário, com questões abertas e fechadas, que foram respondidas por escrito. A nossa amostra, contou com 54 alunos, pertencentes a quatro turmas onde estavam incluídas crianças com NEE.
A análise dos dados permitiu-nos deduzir que os pares aceitam a inclusão das crianças com NEE, no entanto, esta aceitação não é consensual em todas as áreas indispensáveis a uma inclusão de sucesso.
Pese embora a idade das crianças envolvidas, acreditamos que este trabalho abre portas à reflexão em torno da necessidade de se fomentar, desde muito cedo na vida das crianças, valores como a amizade e a igualdade
Riscos da manipulação de citotóxicos
Os medicamentos citotóxicos têm como função a inibição do crescimento e/ou processos vitais das
células tumorais contudo, estes não são selectivos, não atingindo apenas as células afectadas.
Assim, e acrescido ao facto de sabermos que estes são também carcinogénicos, mutagénicos e
teratogénicos para quem os manipula, desenvolvemos este estudo com o objectivo de
percebermos o risco que estes correm.
Para tal, realizamos um estudo descritivo e transversal, onde o instrumento de recolha de dados foi
um inquérito, reunindo assim, uma amostra de 35 profissionais de três hospitais diferentes.
Os dados obtidos permitem-nos concluir que todos os manipuladores de citotóxicos presentes na
amostra, conhecem os efeitos adversos advindos da manipulação, o que significa que todos têm a
percepção do risco que correm.
Com este estudo, pretende-se alertar os profissionais de saúde que contactam directamente com
esta realidade para a execução de boas práticas, a fim de reduzir o risco a que estão expostos
Changes in lignin content of Hypericum perforatum cells after Colletotrichum gloeosporioides elicitation
Fundação para a Ciência e a Tecnologia (FCT) - POCTI/AGR/40283/200
An Insight into the Release Kinetics
The authors acknowledge the financial support from Fundação para a Ciência e a Tecnologia (FCT-MEC), Portugal, through the dedicated project (PTDC/EDM-EDM/30828/2017) (BeLive)
Publisher Copyright:
© 2023 by the authors.This work explores the unique features of magnetic-responsive hydrogels to obtain liposomal hydrogel delivery platforms capable of precise magnetically modulated drug release based on the mechanical responses of these hydrogels when exposed to an external magnetic field. Magnetic-responsive liposomal hydrogel delivery systems were prepared by encapsulation of 1,2-dipalmitoyl-sn-glycero-3-phosphocoline (DPPC) multilayered vesicles (MLVs) loaded with ferulic acid (FA), i.e., DPPC:FA liposomes, into gelatin hydrogel membranes containing dispersed iron oxide nanoparticles (MNPs), i.e., magnetic-responsive gelatin. The FA release mechanisms and kinetics from magnetic-responsive liposomal gelatin were studied and compared with those obtained with conventional drug delivery systems, e.g., free liposomal suspensions and hydrogel matrices, to access the effect of liposome entrapment and magnetic field on FA delivery. FA release from liposomal gelatin membranes was well described by the Korsmeyer–Peppas model, indicating that FA release occurred under a controlled diffusional regime, with or without magnetic stimulation. DPPC:FA liposomal gelatin systems provided smoother controlled FA release, relative to that obtained with the liposome suspensions and with the hydrogel platforms, suggesting the promising application of liposomal hydrogel systems in longer-term therapeutics. The magnetic field, with low intensity (0.08 T), was found to stimulate the FA release from magnetic-responsive liposomal gelatin systems, increasing the release rates while shifting the FA release to a quasi-Fickian mechanism. The magnetic-responsive liposomal hydrogels developed in this work offer the possibility to magnetically activate drug release from these liposomal platforms based on a non-thermal related delivery strategy, paving the way for the development of novel and more efficient applications of MLVs and liposomal delivery systems in biomedicine.publishersversionpublishe
Induction of phenolic compounds in Hypericum perforatum L. cells by colletotrichum gloeosporioides elicitation
Changes in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC–DAD and HPLC–DAD–MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasmonate (MeJ) or salicylic acid (SA), before elicitation. HP cultures exposed only to MeJ produced a set of flavonoids, the flavones which represent a substantial part (approx. 40%) of the total flavonoids accumulated in these cells. The possible importance of xanthones as a component of defence mechanism of HP against biotic stress is discussed.Fundação para a Ciência e a Tecnologia (FCT
Lidocaine supplementation in clove-oil and 2-phenoxyethanol anesthesia for gilthead seabream (Sparus aurata)
This study had the support of Fundaçao ˜ para a Ciˆencia e a Tecnologia (FCT) through the Strategic Project granted to MARE - Marine and Environmental Sciences Centre (https://doi.org/10.54499/UIDB/04
292/2020 and https://doi.org/10.54499/UIDP/04292/2020), the project granted to the Associated Laboratory ARNET (https://doi.org/10.54499/LA/P/0069/2020), the project MAR02.05.01-FEAMP-0013
and CITAB (https://doi.org/10.54499/UIDP/04033/2020.)Com Acordo B-On-Politécnico de Leiria - Elsevier, da lista das Revistas GOA (Aquaculture Reports) na taxa de APC.Animal welfare and reducing stress during procedures are key objectives for success in animal production. Anesthesia has been used for procedures to reduce animal stress and its negative impact on welfare. This study aimed first to refine the concentrations of the anesthetic clove-oil (CO) and lidocaine (L) in gilthead seabream (Sparus aurata) juveniles (56.0 ± 15.09 g) and then combine clove-oil and 2-phenoxyethanol (2PHE) with the refined concentration of lidocaine. The concentrations of clove-oil (30, 45, and 60 mg L− 1), and the concentrations of lidocaine (2.5, 5, and 7.5 mg L− 1), were evaluated in the refinement trial. Based on these results, a second trial was performed with 45 mg L− 1 CO or 0.4 mL L− 1 2PHE as anesthetics alone or combined with 2.5 mg L− 1 of lidocaine. Results from this work showed an improvement in induction times for 2-phenoxyethanol when lidocaine was added (2PHE 179.53 ± 63.21 s; 2PHE + L 130.65 ± 40.16 s). Recovery time also showed a reduction for clove-oil when lidocaine was used (CO 349.90 ± 123.69 s; CO + L 250.11 ± 51.99 s). The use of lidocaine showed better results, reducing lactate and histological progressive alterations. Lidocaine showed stress-induced oxidative alterations when it was combined with 2-phenoxyethanol. Lidocaine exposure increased ALT, AST, histological regressive alterations for both anesthetics, and gene expression of hsp70 in the gills when
clove-oil was used. Further studies are necessary to comprehend the synergistic effects of lidocaine when combined with synthetic and natural anesthetics and to discern potential acute or chronic toxic responses in fish. These insights will be crucial for refining anesthesia protocols and ensuring the well-being of aquatic species in aquaculture practices and research settings.info:eu-repo/semantics/publishedVersio
The Action of Polyphenols in Diabetes Mellitus and Alzheimer's Disease: A Common Agent for Overlapping Pathologies
Diabetes Mellitus (DM) and Alzheimer's disease (AD) are two prevalent diseases in modern societies, which are caused mainly by current lifestyle, aging and genetic alterations. It has already been demonstrated that these two diseases are associated, since individuals suffering from DM are prone to develop AD. Conversely, it is also known that individuals with AD are more susceptible to DM, namely type 2 diabetes (T2DM). Therefore, these two pathologies, although completely different in terms of symptomatology, end up sharing several mechanisms at the molecular level, with the most obvious being the increase of oxidative stress and inflammation. Polyphenols are natural compounds widely spread in fruits and vegetables whose dietary intake has been considered inversely proportional to the incidence of DM and AD. So, it is believed that this group of phytochemicals may have preventive and therapeutic potential, not only by reducing the risk and delaying the development of these pathologies, but also by improving brain's metabolic profile and cognitive function. The aim of this review is to understand the extent to which DM and AD are related pathologies, the degree of similarity and the relationship between them, to detail the molecular mechanisms by which polyphenols may exert a protective effect, such as antioxidant and anti-inflammatory effects, and highlight possible advantages of their use as common preventive and therapeutic alternatives.info:eu-repo/semantics/publishedVersio
Let there be light...
“Let there be light...” is a project in the scope of the International Year of Light 2015 (IYL 2015) targeted at children of the first school grade.
Basically, it comprises nine recreational - scientific workshops that last for approximately 90 minutes. Each workshop is dedicated to a different topic, namely: bioluminescence, mineral observation, the rainbow, light-shadow contrast, battery production, just to name a few, and is designed, in differ- ent approaches, by a distinct team of scientists familiar to the scientific area focused. The activity starts with the dramatized storytelling of a children’s story related to the scientific subject and performed by the team of the public library expert in this area. This moment takes place in an almost magical environment opening the door to the science topic light-related that would be focused later on. In the third part of the workshop, the children are invited to produce plastic works (e.g. drawings, constructions and models) inspired in what they have learned, and that are to be collected in a public exhibition held at the same institution at the end of the project.
In the present work, besides the description of the experience, you can find the critical analysis of the activity and the evaluation of the action by all the actors involved (project team and children/teachers that attended the workshops).info:eu-repo/semantics/publishedVersio
Freeform 3D printing using a continuous viscoelastic supporting matrix
Embedded bio-printing has fostered significant advances toward the fabrication of soft complex tissue-like constructs, by providing a physical support that allows the freeform shape maintenance within the prescribed spatial arrangement, even under gravity force. Current supporting materials still present major drawbacks for up-scaling embedded 3D bio-printing technology towards tissue-like constructs with clinically relevant dimensions. Herein, we report a a cost-effective and widely available supporting material for embedded bio-printing consisting on a continuous pseudo-plastic matrix of xanthan-gum (XG). This natural polisaccharide exhibits peculiar rheological properties that have enabled the rapid generation of complex volumetric 3D constructs with out of plane features. The freedom of design within the three orthogonal axes through the independent and controlled bio-printing process opens new opportunities to produce on demand large arbitrary shapes for personalized medicine. Additionally, we have demonstrated the versatile functionality of XG as a photocurable gel reservoir to engineer perfused cell-laden hydrogel constructs, addressing other practical biomedical applications such as in vitro models and organ-on-chip platforms.publishe
Single-Objective Front Optimization: Application to RF Circuit Design
This paper proposes a new algorithm which promotes well distributed non-dominated fronts in the parameters space when a single- objective function is optimized. This algorithm is based on ǫ-dominance concept and maxmin sorting scheme. Besides that, the paper also presents the results of the algorithm when it is used in the automated synthesis of optimum performance CMOS radiofrequency and microwave binary-weighted differential switched capacitor arrays (RFDSCAs). The genetic synthesis tool optimizes a fitness function which is based on the performance parameter of the RFDSCAs. To validate the proposed design methodology, a CMOS RFDSCA is synthesized, using a 0.25 μm BiCMOS technology.N/
- …