8 research outputs found
Endocytosis of coacervates into liposomes
[Image: see text] Recent studies have shown that the interactions between condensates and biological membranes are of functional importance. Here, we study how the interaction between complex coacervates and liposomes as model systems can lead to wetting, membrane deformation, and endocytosis. Depending on the interaction strength between coacervates and liposomes, the wetting behavior ranged from nonwetting to engulfment (endocytosis) and complete wetting. Endocytosis of coacervates was found to be a general phenomenon: coacervates made from a wide range of components could be taken up by liposomes. A simple theory taking into account surface energies and coacervate sizes can explain the observed morphologies. Our findings can help to better understand condensateâmembrane interactions in cellular systems and provide new avenues for intracellular delivery using coacervates
Endocytosis of coacervates into liposomes
Recent studies have shown that the interactions between condensates and biological membranes is of functional importance. Here, we study how the interaction between complex coacervates and liposomes as model systems can lead to membrane deformation and endocytosis. Depending on the interaction strength between coacervates and liposomes, the wetting behavior ranged from non-wetting, to partial wetting (adhesion), engulfment (endocytosis), and finally complete wetting. Endocytosis of coacervates was found to be a general phenomenon: coacervates made from a wide range of components could be taken up by liposomes. A simple theory that takes into account surface energies and coacervate sizes can explain the observed coacervate-liposome interactions. Our findings can help to better understand condensate-membrane interactions in cellular systems and provide new avenues for intracellular delivery using coacervates
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Improvements and Applications of AMS Radiocarbon Measurement at Peking University
AMS radiocarbon measurements were started at Peking University in 1992 with a modified HICONEX 834 ion source. Some archaeological samples were measured at a sensitivity of 10^-14 with ca. 1.7% precision for modern samples. We have made many improvements in our first two years of operation: a high-intensity Cs sputtering ion source was installed; the graphite sample preparation technique was investigated; and the system stability has been improved. The blank sample background is currently ca. 0.006 MC and a precision within 1% can be reached for modern samples. Geological, archaeological, environmental and biomedical samples can be measured routinely. We present some typical applications.This material was digitized as part of a cooperative project between Radiocarbon and the University of Arizona Libraries.The Radiocarbon archives are made available by Radiocarbon and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform February 202
Bone Marrow Mesenchymal Stem CellâDerived DermcidinâContaining Migrasomes enhance LC3âAssociated Phagocytosis of Pulmonary Macrophages and Protect against PostâStroke Pneumonia
Abstract Pneumonia is one of the leading causes of death in patients with acute ischemic stroke (AIS). Antibiotics fail to improve prognosis of patients with postâstroke pneumonia, albeit suppressing infection, due to adverse impacts on the immune system. The current study reports that bone marrow mesenchymal stem cells (BMâMSC) downregulate bacterial load in the lungs of stroke mice models. RNAâsequencing of the lung from BMâMSCâtreated stroke models indicates that BMâMSC modulates pulmonary macrophage activities after cerebral ischemia. Mechanistically, BMâMSC promotes the bacterial phagocytosis of pulmonary macrophages through releasing migrasomes, which are migrationâdependent extracellular vesicles. With liquid chromatographyâtandem mass spectrometry (LCâMS/MS), the result shows that BMâMSC are found to load the antibacterial peptide dermcidin (DCD) in migrasomes upon bacterial stimulation. Besides the antibiotic effect, DCD enhances LC3âassociated phagocytosis (LAP) of macrophages, facilitating their bacterial clearance. The data demonstrate that BMâMSC is a promising therapeutic candidate against postâstroke pneumonia, with dual functions of antiâinfection and immunol modulation, which is more than a match for antibiotics treatment
In vivo anti-hyperuricemia and anti-gouty arthritis effects of the ethanol extract from Amomumvillosum Lour.
The incidence of hyperuricemia and gout has been increasing year by year, and it is showing a younger trend. However, the first-line drugs currently used for hyperuricemia and gouty arthritis have serious side effects that limit their clinical application. Amomum villosum Lour. has been widely used in China for thousands of years as a traditional medical and edible plant, and previous screening showed that the ethanol extract of Amomum villosum Lour. could effectively inhibit the activity of xanthine oxidase. Based on this discovery, this paper had achieved in-depth mechanism research. The results showed that the ethanol extract of Amomum villosum Lour. could treat hyperuricemia by reducing the production of uric acid via inhibition of xanthine oxidase and increasing the excretion of uric acid via regulation of urate transporters. Meanwhile, the extract also showed a certain protective effect on hepatic and renal damage caused by hyperuricemia. With the formation of extensive uric acid, gouty arthritis will be induced by the deposition of monosodium urate in the joint. The extract could also relieve the inflammation by reducing the expression of inflammatory cytokines. In conclusion, the extract deserves focused research and development as a potential medicine, health care product or supplemented food for the prevention and treatment of hyperuricemia and gouty arthritis