Producción científica enfermera del Hospital Infanta Elena de Huelva durante los años 1985-2009

The aim of this paper is to describe the scientific production of nursing staff of the “Infanta Elena” Hospital (Huelva) for the years 1985 to 2009. We proposed a search strategy in the Cuiden Plus database and extracted all references to the conditions described. 68 documents were recovered. 35% of the articles were published in local journals. 80% were published in journals without impact. 65% of the documents are citable (original or review). A total of 98 authors have signed all the papers, of which 67 are occasional producers. The most common theme focuses on Nursing and Nursing Care. There is a lack of cooperation both at the institutionally and in terms of authorship. There has been an exponential growth of publicationsEl presente trabajo tiene como finalidad describir la producción científica del personal de Enfermería del Hospital Infanta Elena de Huelva durante los años 1985 al 2009. Para ello se plantea una estrategia de búsqueda en la base de datos Cuiden Plus y se extraen todas las referencias bibliográficas que cumplen las condiciones descritas. Se recuperan un total de 68 documentos. El 35% de los artículos se publican en revistas de ámbito local. El 80% se publica en revistas sin Impacto. El 65% de los documentos son citables (originales o revisión). Un total de 98 autores firman todos los artículos, de los que el 67 son productores ocasionales. La temática más habitual se centra en la Enfermería y en los cuidados de Enfermería. Hay una escasa colaboración tanto institucional como a nivel de autoría. Se constata un crecimiento exponencial de las publicaciones

Supply chain analysis by ABC classification: the case of a Mexican company

There is a direct relation between products’ shelf life and their sales volume. The analysis of these factors is important for logistic planning, since products’ positioning in the warehouse must facilitate their distribution according to their rotation in the inventory. The analysis of supply chain costs is relevant to identify the items that most contribute to a company’s profit. This study objective is to identify the items with the highest value of inventory of Mexican company BASAL by classifying them into categories according to the logics of ABC analysis. Results showed that 80% of sales are concentrated in 28 articles, which represent 22% of the company’s products. Attention must be given to the supply of articles with slower movement because they would also increase total sales

Experimental study of the effect of temperature on the strength of ready-mixed concrete : theory

En este artículo se presenta un estudio sobre los efectos de la temperatura ambiental sobre las prestaciones del hormigón, en estado fresco y endurecido, y sobre las propiedades de los materiales constituyentes del mismo, desde una perspectiva industrial. El objetivo principal es el de optimizar la dosificación del hormigón en climas cálidos y al mismo tiempo, establecer medidas de actuación a nivel industrial para minimizar los efectos adversos en plantas de producción de hormigón preparado. En la primera parte del artículo, se muestran los resultados de varias campañas experimentales cuyo propósito ha sido determinar la influencia de las temperaturas ambientales extremas sobre las propiedades térmicas, de trabajabilidad y mecánicas del hormigón, así como de morteros, pastas de cemento y áridos. Las conclusiones apuntan al árido como un factor de gran importancia, no sólo por las características específicas de su comportamiento con la temperatura, sino también, porque es el componente presente en mayor contenido en el hormigón. En la segunda parte del artículo, se presentan las bases de una formulación metodológica para llevar a cabo la optimización del hormigón en climas cálidos

Distinct expression patterns predict differential roles of the miRNA-binding proteins, Lin28 and Lin28b, in the mouse testis: studies during postnatal development and in a model of hypogonadotropic hypogonadism

[Abstract] Lin28 (also termed Lin28a) and Lin28b are related RNA-binding proteins, involved in the control of microRNA synthesis, especially of the let-7 family, with putative functions in early (embryo) development. However, their roles during postnatal maturation remain ill defined. Despite the general assumption that Lin28 and Lin28b share similar targets and functions, conclusive demonstration of such redundancy is still missing. In addition, recent observations suggest a role of Lin28 proteins in mammalian reproduction, which is yet to be defined. We document herein the patterns of RNA expression and protein distribution of Lin28 and Lin28b in mouse testis during postnatal development and in a model of hypogonadotropic hypogonadism as a result of inactivation of the kisspeptin receptor, Gpr54. Lin28 and Lin28b mRNAs were expressed in mouse testis across postnatal maturation, but their levels disparately varied between neonatal and pubertal periods, with peak Lin28 levels in infantile testes and sustained elevation of Lin28b mRNA in young adult male gonads, where relative levels of let-7a and let-7b miRNAs were significantly suppressed. In addition, Lin28 peptides displayed totally different patterns of cellular distribution in mouse testis: Lin28 was located in undifferentiated and type-A1 spermatogonia, whereas Lin28b was confined to spermatids and interstitial Leydig cells. These profiles were perturbed in Gpr54 null mouse testis, which showed preserved but irregular Lin28 signal and absence of Lin28b peptide, which was rescued by administration of gonadotropins, mainly hCG (as super-agonist of LH). In addition, increased relative levels of Lin28, but not Lin28b, mRNA and of let-7a/let-7b miRNAs were observed in Gpr54 KO mouse testes. Altogether, our data are the first to document the divergent patterns of cellular distribution and mRNA expression of Lin28 and Lin28b in the mouse testis along postnatal maturation and their alteration in a model of congenital hypogonadotropic hypogonadism. Our findings suggest distinct functional roles of these two related, but not overlapping, miRNA-binding proteins in the male gonad.Ministerio de Ciencia e Innovación; BFU 2008-00984Ministerio de Ciencia e Innovación; BFU2011-25021Junta de Andalucía; Project P08-CVI-03788European Commission; DEER FP7-ENV-2007-

Extracellular NK histones promote immune cell anti-tumor activity by inducing cell clusters through binding to CD138 receptor

Background: Natural killer (NK) cells are important anti-tumor cells of our innate immune system. Their anti-cancer activity is mediated through interaction of a wide array of activating and inhibitory receptors with their ligands on tumor cells. After activation, NK cells also secrete a variety of pro-inflammatory molecules that contribute to the final immune response by modulating other innate and adaptive immune cells. In this regard, external proteins from NK cell secretome and the mechanisms by which they mediate these responses are poorly defined. Methods: TRANS-stable-isotope labeling of amino acids in cell culture (TRANS-SILAC) combined with proteomic was undertaken to identify early materials transferred between cord blood-derived NK cells (CB-NK) and multiple myeloma (MM) cells. Further in vitro and in vivo studies with knock-down of histones and CD138, overexpression of histones and addition of exogenous histones were undertaken to confirm TRANS-SILAC results and to determine functional roles of this material transferred. Results: We describe a novel mechanism by which histones are actively released by NK cells early after contact with MM cells. We show that extracellular histones bind to the heparan sulfate proteoglycan CD138 on the surface of MM cells to promote the creation of immune-tumor cell clusters bringing immune and MM cells into close proximity, and thus facilitating not only NK but also T lymphocyte anti-MM activity. Conclusion: This study demonstrates a novel immunoregulatory role of NK cells against MM cells mediated by histones, and an additional role of NK cells modulating T lymphocytes activity that will open up new avenues to design future immunotherapy clinical strategies

Influencia de la temperatura ambiental en las propiedades del concreto hidráulico

Durante los meses de verano el concreto premezclado sufre efectos negativos sobre sus prestaciones en estado fresco y endurecido. En este trabajo se presentan los resultados obtenidos en un proyecto de investigación cuyo objetivo principal es el de optimizar la dosificación de cemento en el concreto fabricado bajo condiciones elevadas de temperatura. Los estudios realizados comprenden principalmente campañas experimentales con concreto y con agregados, afectados por situaciones climáticas específicas, simulando condiciones de verano e invierno. Las conclusiones finales dan pauta a potenciales aplicaciones industriales con el fin de disminuir los efectos negativos sobre el concreto y de generar beneficios económicos y medioambientales

Extracellular NK histones promote immune cell anti-tumor activity by inducing cell clusters through binding to CD138 receptor.

BACKGROUND: Natural killer (NK) cells are important anti-tumor cells of our innate immune system. Their anti-cancer activity is mediated through interaction of a wide array of activating and inhibitory receptors with their ligands on tumor cells. After activation, NK cells also secrete a variety of pro-inflammatory molecules that contribute to the final immune response by modulating other innate and adaptive immune cells. In this regard, external proteins from NK cell secretome and the mechanisms by which they mediate these responses are poorly defined. METHODS: TRANS-stable-isotope labeling of amino acids in cell culture (TRANS-SILAC) combined with proteomic was undertaken to identify early materials transferred between cord blood-derived NK cells (CB-NK) and multiple myeloma (MM) cells. Further in vitro and in vivo studies with knock-down of histones and CD138, overexpression of histones and addition of exogenous histones were undertaken to confirm TRANS-SILAC results and to determine functional roles of this material transferred. RESULTS: We describe a novel mechanism by which histones are actively released by NK cells early after contact with MM cells. We show that extracellular histones bind to the heparan sulfate proteoglycan CD138 on the surface of MM cells to promote the creation of immune-tumor cell clusters bringing immune and MM cells into close proximity, and thus facilitating not only NK but also T lymphocyte anti-MM activity. CONCLUSION: This study demonstrates a novel immunoregulatory role of NK cells against MM cells mediated by histones, and an additional role of NK cells modulating T lymphocytes activity that will open up new avenues to design future immunotherapy clinical strategies

Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product

Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as almost complete agreement.info:eu-repo/semantics/publishedVersio

Moderate Hypercalcemia Secondary to Intrathyroidal Parathyroid Adenoma: Case Report

El hiperparatiroidismo (HP) es la sobreproducción de Hormona Paratiroidea (PTH) producida por las glándulas paratiroideas, dividiéndose en: primario, secundario y terciario. El HP primario, nos habla de la función aumentada de una o más glándulas paratiroideas, secretando PTH de forma excesiva. El adenoma único es la principal causa de HP primario. Su presentación clínica en 80-90% de los casos es asintomática. El estándar de oro es el tratamiento quirúrgico mediante la exploración cervical bilateral para permitir al cirujano mejor visibilidad y movilidad del cuello, sin embargo, deben realizarse estudios de imagen para determinar la localización de la lesión. Se ha reportado una tasa de curaciones cercana al 95% en pacientes sometidos a paratiroidectomía.&nbsp;Hyperparathyroidism (HP) is the overproduction of thyroid hormone produced by the parathyroid glands, divided into: primary, secondary and tertiary. Primary HP is the increased function of one or more parathyroid glands, secreting Parathyroid Hormone (PTH) excessively, with the single adenoma being the principal cause of primary HP. The clinical presentation in 80-90% of cases is asymptomatic. The gold standard is surgical treatment through bilateral cervical exploration to allow the surgeon visibility and mobility of the neck, however, imagine studies must be performed to determine the location of the lesion. A cure rate close to 95% has been reported in patients undergoing parathyroidectomy.&nbsp