14,502 research outputs found
Further Characterization of Dopamine Release by Permeabilized PC 12 Cells
Rat pheochromocytoma cells (PC 12) permeabilized with staphylococcal α-toxin release [3H]dopamine after addition of micromolar Ca2+. This does not require additional Mg2+-ATP (in contrast to bovine adrenal medullary chromaffin cells). We also observed Ca2+-dependent [3H]-dopamine release from digitonin-permeabilized PC 12 cells. Permeabilization with α-toxin or digitonin and stimulation of the cells were done consecutively to wash out endogenous Mg2+-ATP. During permeabilization, ATP was removed effectively from the cytoplasm by both agents but the cells released [3H]dopamine in response to micromolar Ca2+ alone. Replacement by chloride of glutamate, which could sustain mitochondrial ATP production in permeabilized cells, does not significantly alter catecholamine release induced by Ca2+. However, Mg2+ without ATP augments the Ca2+-induced release. The release was unaltered by thiol-, hydroxyl-, or calmodulin-interfering substances. Thus Mg2+-ATP, calmodulin, or proteins containing -SH or -OH groups are not necessary for exocytosis in permeabilized PC 12 cells
Chiral molecules split light: Reflection and refraction in a chiral liquid
A light beam changes direction as it enters a liquid at an angle from another
medium, such as air. Should the liquid contain molecules that lack mirror
symmetry, then it has been predicted by Fresnel that the light beam will not
only change direction, but will actually split into two separate beams with a
small difference in the respective angles of refraction. Here we report the
observation of this phenomenon. We also demonstrate that the angle of
reflection does not equal the angle of incidence in a chiral medium. Unlike
conventional optical rotation, which depends on the path-length through the
sample, the reported reflection and refraction phenomena arise within a few
wavelengths at the interface and thereby suggest a new approach to polarimetry
that can be used in microfluidic volumes
Bingham Dai, Adolf Storfer, and the tentative beginnings of psychoanalytic culture in China, 1935-1941.
This paper looks at the work of two figures who, while marginal to theoretical developments within the history of psychoanalysis, each briefly played an important role in the dissemination of analytical ideas in China, contributing to an early psychoanalytic culture there. Bingham Dai, a native of China, while studying for a PhD in sociology at Chicago, received instruction from Harry Stack Sullivan and a psychoanalytic training under Karen Horney's supervision. However, the neo-Freudian outlook with which this experience imbued him had its roots in an earlier encounter with his experiments in personality education first conducted on students in a Tientsin high school, and later in Shantung under the direction of the conservative Confucian scholar and reformer, Liang Shu Ming. These experiences convinced him that a less orthodox psychoanalytic perspective was what Chinese patients with psychological problems required. He returned in 1935 to teach medical psychology to doctors at Peking Union Medical College, taking a few into analysis and treating some patients. However, the Sino-Japanese war brought these activities to a close and he left in 1939, just a few months after the former Freud publisher and Viennese émigré, Adolf Storfer, arrived. Storfer set about publishing "Gelbe Post," a German language periodical replete with articles on psychoanalysis, linguistics and Chinese culture. But limited finances, severe competition from a rival publisher, plus his own ill health, forced him to abandon this in spite of the support offered him through the many contributors in the international psychoanalytic community whose articles he published. The paper concludes by considering the relative historiographic fate of the men upon whom subsequent scholarship has been very unevenly focused.published_or_final_versio
Effects of Monovalent and Divalent Cations on Ca2+ Fluxes Across Chromaffin Secretory Membrane Vesicles
Abstract: Bovine chromaffin secretory vesicle ghosts loaded with Na+ were found to take up Ca2+ when incubated in K+ media or in sucrose media containing micromolar concentrations of free Ca2+. Li+- or choline+loaded ghosts did not take up Ca2+. The Ca2+ accumulated by Na+-loaded ghosts could be released by the Ca2+ ionophore A23187, but not by EGTA. Ca2+ uptake was inhibited by external Sr2+, Na +, Li +, or choline +. All the 45Ca2+ accumulated by Na+-dependent Ca2+ uptake could be released by external Na +, indicating that both Ca2+ influx and efflux occur in a Na+-dependent manner. Na + -dependent Ca2+ uptake and release were only slightly inhibited by Mg2+. In the presence of the Na+ ionophore Monensin the Ca2+ uptake by Na +-loaded ghosts was reduced. Ca2+ sequestered by the Na+-dependent mechanism could also be released by external Ca2+ or Sr2+ but not by Mg2+, indicating the presence of a Ca2+/Ca2+ exchange activity in secretory membrane vesicles. This Ca2+/Ca2+ exchange system is inhibited by Mg2+, but not by Sr2+. The Na + -dependent Ca2+ uptake system in the presence of Mg2+ is a saturable process with an apparent Km of 0.28 μM and a Vmax= 14.5 nmol min−1 mg protein−1. Ruthenium red inhibited neither the Na+/Ca2+ nor the Ca2+/Ca2+ exchange, even at high concentrations
Peroxisomes in intestinal and gallbladder epithelial cells of the stickleback, Gasterosteus aculeatus L. (Teleostei)
The occurrence of microbodies in the epithelial cells of the intestine and gallbladder of the stickleback, Gasterosteus aculeatus L., is described. In the intestine the organelles are predominantly located in the apical and perinuclear zone of the cells and may contain small crystalline cores. In gallbladder epithelial cells the microbodies are distributed randomly. The latter organdies are characterized by the presence of large crystalloids. Cytochemical and biochemical experiments show that catalase and D-amino acid oxidase are main matrix components of the microbodies in both the intestinal and gallbladder epithelia. These organelles therefore are considered peroxisomes. In addition, in intestinal mucosa but not in gallbladder epithelium a low activity of palmitoyl CoA oxidase was detected biochemically. Urate oxidase and L-α hydroxy acid oxidase activities could not be demonstrated.
Spitzer observations of the asteroid-comet transition object and potential spacecraft target 107P (4015) Wilson-Harrington
Context. Near-Earth asteroid-comet transition object 107P/ (4015)
Wilson-Harrington is a possible target of the joint European Space Agency (ESA)
and Japanese Aerospace Exploration Agency (JAXA) Marco Polo sample return
mission. Physical studies of this object are relevant to this mission, and also
to understanding its asteroidal or cometary nature. Aims. Our aim is to obtain
significant new constraints on the surface thermal properties of this object.
Methods. We present mid-infrared photometry in two filters (16 and 22 microns)
obtained with NASA's Spitzer Space Telescope on February 12, 2007, and results
from the application of the Near Earth Asteroid Thermal Model (NEATM).We
obtained high S/N in two mid-IR bands allowing accurate measurements of its
thermal emission. Results. We obtain a well constrained beaming parameter (eta
= 1.39 +/- 0.26) and obtain a diameter and geometric albedo of D = 3.46 +/-
0.32 km, and pV = 0.059 +/- 0.011. We also obtain similar results when we apply
this best-fitting thermal model to single-band mid-IR photometry reported by
Campins et al. (1995), Kraemer et al. (2005) and Reach et al. (2007).
Conclusions. The albedo of 4015 Wilson-Harrington is low, consistent with those
of comet nuclei and primitive C-, P-, D-type asteorids. We establish a rough
lower limit for the thermal inertia of W-H of 60 Jm^-2s^(-0.5)K^-1 when it is
at r=1AU, which is slightly over the limit of 30 Jm^-2s^(-0.5)K-1 derived by
Groussin et al. (2009) for the thermal inertia of the nucleus of comet
22P/Kopff.Comment: 4 pages, 1 figure and 3 tables. Paper accepted for publicatio
Circadian rhythm of hepatic cytosolic and nuclear estrogen receptors
The distribution of estrogen receptor between the cytosolic and nuclear compartments were evaluated in liver of male rats to determine whether a circadian rhythm exists. Cytosolic receptor reached a maximum level at 400 hours and a minimum at 2000 and 2400 hr. Nuclear receptor reached a maximum level at 800 hr and was lowest at 1600 and 2000 hr. Serum estradiol levels were also highest at 800 hr and lowest at 1600 hr. The variations in cytosolic and nuclear receptors are not reciprocal; in fact, the overall content of receptor in the liver is not constant and also displays a circadian rhythm. © 1986 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted
A proposal for ethical research conduct in Madagascar
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. The attached file is the published version of the article
A bulk superconducting MgB2 cylinder for holding transversely polarized targets
An innovative solution is being pursued for the challenging magnetic problem of producing an internal transverse field around a polarized target, while shielding out an external longitudinal field from a detector. A hollow bulk superconductor can trap a transverse field that is present when cooled through its transition temperature, and also shield its interior from any subsequent field changes. A feasibility study with a prototype bulk MgB2 superconducting cylinder is described. Promising measurements taken of the interior field retention and exterior field exclusion, together with the corresponding long-term stability performance, are reported. In the context of an electron scattering experiment, such a solution minimizes beam deflection and the energy loss of reaction products, while also eliminating the heat load to the target cryostat from current leads that would be used with conventional electromagnets
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