Momentum Transfer Dependence of Nuclear Transparency from the Quasielastic ^(12)C(e, e'p) Reaction

The cross section for quasielastic ^(12)C(e,e’p) scattering has been measured at momentum transfer Q^2=1, 3, 5, and 6.8 (GeV/c)^2. The results are consistent with scattering from a single nucleon as the dominant process. The nuclear transparency is obtained and compared with theoretical calculations that incorporate color transparency effects. No significant rise of the transparency with Q^2 is observed

Mudbricks, Construction Methods, and Stratigraphic Analysis : A Case Study at Tell Timai (ancient Thmuis) in the Egyptian Delta

The Graeco-Roman site of Tell Timai (ancient Thmuis) in Lower Egypt is among the largest urban tells in the Nile Delta, boasting substantial amounts of preserved earthen architecture. Although earthen architecture made up the vast majority of public and domestic structures in ancient Egypt, it still does not receive the same analytical attention from archaeologists as other categories of evidence. This paper presents a case study for the archaeological investigation of the earthen architecture at Tell Timai. The goal was to develop a methodology that can be implemented in the field by excavators with little geoarchaeological training and limited laboratory access in order to generate useful data for determining building stratigraphy and studying construction processes. Through the close examination and sampling of three buildings of different periods and scales, we tested a new field methodology combining geoarchaeological techniques and mensiochronology. The results provide information useful for stratigraphy and phasing as well as for identifying specific patterns of mudbrick manufacturing, production, and construction during the Graeco-Roman period at Tell Timai.Peer reviewe

Microglia in prion diseases: Angels or demons?

Prion diseases are rare transmissible neurodegenerative disorders caused by the accumulation of a misfolded isoform (PrPSc) of the cellular prion protein (PrPC) in the central nervous system (CNS). Neuropathological hallmarks of prion diseases are neuronal loss, astrogliosis, and enhanced microglial proliferation and activation. As immune cells of the CNS, microglia participate both in the maintenance of the normal brain physiology and in driving the neuroinflammatory response to acute or chronic (e.g., neurodegenerative disorders) insults. Microglia involvement in prion diseases, however, is far from being clearly understood. During this review, we summarize and discuss controversial findings, both in patient and animal models, suggesting a neuroprotective role of microglia in prion disease pathogenesis and progression, or\u2014conversely\u2014a microglia-mediated exacerbation of neurotoxicity in later stages of disease. We also will consider the active participation of PrPC in microglial functions, by discussing previous reports, but also by presenting unpublished results that support a role for PrPC in cytokine secretion by activated primary microglia

Formation et solidification de la zone fondue en soudage par point : influence des paramètres de soudage

National audienceLe soudage par point est un procédé d'assemblage très rapide et très largement utilisé dans l'industrie automobile. Mieux comprendre la genèse de la zone fondue de points soudés et en particulier sa solidification doit permettre d'améliorer les performances mécaniques des points soudés tout en fournissant des recommandations pour optimiser le cycle de soudage. Dans cette optique, plusieurs nuances d'aciers doux et à très haute résistance, d'épaisseurs 1 et 2 mm ainsi que leurs assemblages hétérogènes ont été testés. Les cinétiques de croissance de la zone fondue en fonction de la composition chimique ont été déterminées grâce à des cycles de soudage interrompus suivis d'une trempe. Cette technique a permis de démontrer qu'en conditions de soudage hétérogène, le métal fondu s'homogénéise par convection. Il a été également mis en évidence qu'un regime transitoire de solidification de la zone fondue débute bien avant l'arrêt du courant. Un modèle de croissance dendritique, qui est le mode de solidification habituellement rencontré en soudage par point, a été développé pour analyser la solidification de la zone fondue. Ce modèle de croissance dendritique, couplé à une modélisation des transferts thermiques, permet d'estimer la dimension des microstructures dendritiques du noyau en fonction des conditions locales de refroidissement. Les prévisions du modèle de croissance dendritique sont en bon accord avec les valeurs des espacements dendritiques mesurés

P216 Comparative Assessment C-reactive Protein Between a Point-of-Care Testing and Current Standard of Care (Immunonephelometric testing)

Abstract Background C-reactive protein (CRP) is widely used as a biomarker of inflammatory disease activity in hospitalized and non-hospitalized patients. In particular, CRP is commonly used in patients suspected to have an inflammatory bowel disease (IBD) or with a confirmed diagnosis of IBD diagnosis in order to drive the diagnostic approach, to monitor disease activity and to guide therapeutic adjustments. However, standard laboratory CRP testing (Immunonephelometric assays) present some drawbacks, including a turnaround time of 1–2 hours, and the need of specialized equipment, offices and laboratory personnel. Because of that, point-of care testing (POCT) was recently developed in order to provide results within 2 minutes from blood collection, enabling a rapid response to clinical condition. Aim To determine the degree of analytical correlation between a recently developed POCT (ProciseDx) using capillary whole blood and the comparative Immunonephelometric assay using serum samples. Methods From October to November 2020, consecutive patients hospitalized at Gastroenterology Unit, Padua University Hospital, aged > 18 years and with clinical evidence of active inflammatory disease or infection, who underwent to a standard of care CRP test (Dimension Vista – Siemens Healthineers) were included in the study (range 2.9–340 g/L). Within 1 hour from blood collection, in each patient, CRP quantitation from capillary whole blood collected by finger stick was performed using the ProciseDx CRP assay, with reportable range between 3.6–100 g/L. A Deming regression test was used to identify the correlation between the two methods. Results Eighty-three patients were enrolled (62.5% males with mean age ± SD: 60±18). The most common indications for hospitalisation were liver disease (34.9%), pancreatic disturbance (27.7%) and suspicious or recurrence of IBD (16.7%). ProciseDx POCT with finger prick samples required a turnaround time of 2±0.2 minutes, whereas serum samples analyzed in clinical laboratory with the reference method required a turnaround time of about 180±15 minutes (p<0.001). Overall, the correlation between the two tests was high (R squared of 0.899 (95% CI 0.916–0.968)). In particular, the correlation between the methods was even higher with CRP values between 0–100 g/L with R squared of 0.961 (95% CI 0.958–0.986). Conclusion The ProciseDx POCT allows a more rapid and comparable accuracy of CRP assessment in hospitalized patients as compared to the standard laboratory measurement. Moreover, the ProciseDx POCT does not require specialised personnel to be performed. The use of ProciseDx POCT may improve and accelerate the decision-making approach, further reducing the resources required for CRP assessment

Neuronal Agrin Promotes Proliferation of Primary Human Myoblasts in an Age-Dependent Manner

Neuronal agrin, a heparan sulphate proteoglycan secreted by the -motor neurons, promotes the formation and maintenance of the neuromuscular junction by binding to Lrp4 and activating muscle-specific kinase (MuSK). Neuronal agrin also promotes myogenesis by enhancing differentiation and maturation of myotubes, but its effect on proliferating human myoblasts, which are often considered to be unresponsive to agrin, remains unclear. Using primary human myoblasts, we determined that neuronal agrin induced transient dephosphorylation of ERK1/2, while c-Abl, STAT3, and focal adhesion kinase were unresponsive. Gene silencing of Lrp4 and MuSK markedly reduced the BrdU incorporation, suggesting the functional importance of the Lrp4/MuSK complex for myoblast proliferation. Acute and chronic treatments with neuronal agrin increased the proliferation of human myoblasts in old donors, but they did not affect the proliferation of myoblasts in young donors. The C-terminal fragment of agrin which lacks the Lrp4-binding site and cannot activate MuSK had a similar age-dependent effect, indicating that the age-dependent signalling pathways activated by neuronal agrin involve the Lrp4/MuSK receptor complex as well as an Lrp4/MuSK-independent pathway which remained unknown. Collectively, our results highlight an age-dependent role for neuronal agrin in promoting the proliferation of human myoblasts