Is there a correlation between self-reported level of activity and observed physical performance in elderly women?

The aim of the study was to verify the degree of association between self-reported level of physical activity and observed physical performance of aged women. Thirty-seven women (mean aged 72.7±5.99 years old) were selected by looking up admission records in a reference centre for physical rehabilitation. The Human Activity Profile (HAP) was used to measure activity level; and, for direct observation of physical performance, the six-minute walk test (6MWT) and the 30-second chair-stand test were applied. A statistically significant (p=0.01), weak positive correlation (r=0.41) was found between HAP scores and 6MWT values; and an association trend was noticed between the classification of activity level and the 6MWT variable (p=0.08). Also, a significant (p=0.02), weak positive correlation (r=0.36) was found between HAP scores and results of the 30-second chair stand test. It may be said there is a correlation, though weak, between self-reported and observed levels of physical performance among the elderly here studied. Further research should address aged community dwellers - not only rehabilitation patients - in order to assess a possible under or overestimation of the physical activity level in self-reported evaluations.Este estudo visou verificar se havia associação entre o nível de atividade físico-funcional auto-referido e o desempenho físico de idosas em centro de reabilitação. Foram selecionadas 37 mulheres idosas (com em média 72,7±5,9 anos) pelas fichas de admissão em serviço de atividade física de um centro de referência em reabilitação. Para a avaliação do nível de atividade física auto-referido empregou-se o Perfil de Atividade Humana (PAH); na observação direta de desempenho físico aplicaram-se os testes de caminhada em 6 minutos (TC6') e levantar da cadeira em 30 segundos (TSL30). Foi encontrada correlação positiva fraca (r=0,41) e estatisticamente significante (p=0,01) entre os valores do PAH e o TC6'; e uma tendência de associação entre a classificação do nível de atividade e a variável categórica TC6' (p=0,08). Também foi encontrada correlação positiva fraca (r=0,36) e estatisticamente significante (p=0,02) entre o PAH e o TSL30. Pode-se afirmar que o auto-relato de nível de atividade física das idosas estudadas correlaciona-se, porém fracamente, ao desempenho físico observado. Sugerem-se novas pesquisas com amostras representativas da população idosa em uma comunidade, não apenas de pacientes de centro de reabilitação, para averiguar possíveis tendências à subestimação ou superestimação do nível de atividade física em avaliações auto-referidas.UNIFESP-EPM Lar Escola São FranciscoUNIFESP, EPM, Lar Escola São FranciscoSciEL

Há correlação entre o nível de atividade auto-referido e desempenho físico observado em mulheres idosas?

The aim of the study was to verify the degree of association between self-reported level of physical activity and observed physical performance of aged women. Thirty-seven women (mean aged 72.7±5.99 years old) were selected by looking up admission records in a reference centre for physical rehabilitation. The Human Activity Profile (HAP) was used to measure activity level; and, for direct observation of physical performance, the six-minute walk test (6MWT) and the 30-second chair-stand test were applied. A statistically significant (p=0.01), weak positive correlation (r=0.41) was found between HAP scores and 6MWT values; and an association trend was noticed between the classification of activity level and the 6MWT variable (p=0.08). Also, a significant (p=0.02), weak positive correlation (r=0.36) was found between HAP scores and results of the 30-second chair stand test. It may be said there is a correlation, though weak, between self-reported and observed levels of physical performance among the elderly here studied. Further research should address aged community dwellers - not only rehabilitation patients - in order to assess a possible under or overestimation of the physical activity level in self-reported evaluations.Este estudo visou verificar se havia associação entre o nível de atividade físico-funcional auto-referido e o desempenho físico de idosas em centro de reabilitação. Foram selecionadas 37 mulheres idosas (com em média 72,7±5,9 anos) pelas fichas de admissão em serviço de atividade física de um centro de referência em reabilitação. Para a avaliação do nível de atividade física auto-referido empregou-se o Perfil de Atividade Humana (PAH); na observação direta de desempenho físico aplicaram-se os testes de caminhada em 6 minutos (TC6') e levantar da cadeira em 30 segundos (TSL30"). Foi encontrada correlação positiva fraca (r=0,41) e estatisticamente significante (p=0,01) entre os valores do PAH e o TC6'; e uma tendência de associação entre a classificação do nível de atividade e a variável categórica TC6' (p=0,08). Também foi encontrada correlação positiva fraca (r=0,36) e estatisticamente significante (p=0,02) entre o PAH e o TSL30". Pode-se afirmar que o auto-relato de nível de atividade física das idosas estudadas correlaciona-se, porém fracamente, ao desempenho físico observado. Sugerem-se novas pesquisas com amostras representativas da população idosa em uma comunidade, não apenas de pacientes de centro de reabilitação, para averiguar possíveis tendências à subestimação ou superestimação do nível de atividade física em avaliações auto-referidas

Candida tropicalis as a promising oleaginous yeast for olive mill wastewater bioconversion

Olive mill wastewater (OMW), which is generated during olive oil production, has detrimental effects on the environment due to its high organic load and phenolic compounds content. OMW is difficult to biodegrade, but represents a valuable resource of nutrients for microbial growth. In this study, yeast strains were screened for their growth on phenolic compounds usually found in OMW and responsible for antimicrobial effects. Candida tropicalis ATCC 750 demonstrated an extraordinary capacity to grow in phenolics and was chosen for further experiments with OMW-based medium. The effects of nitrogen supplementation, the pH, and the stirring rate on cellular growth, OMW-components consumption, and added-value compounds production were studied in batch cultures in Erlenmeyer flasks and in a bioreactor. Candida tropicalis was able to reduce 68% of the organic load (chemical oxygen demand) and 39% of the total phenols of OMW in optimized conditions in bioreactor experiments, producing lipase (203 U·L−1) and protease (1105 U·L−1). Moreover, intracellular lipids were accumulated, most significantly under nitrogen-limited conditions, which is common in this type of wastewater. The high potential of C. tropicalis to detoxify OMW and produce added-value compounds from it makes this process an alternative approach to other conventional processes of OMW treatment.This research was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project TUBITAK 2014 (TUBITAK/0009/2014), the strategic funding of the UIDB/04469/2020 unit, a doctoral grant (SFRH/BD/129592/2017), and the BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersio

Synergistic Effect of Adjustments of Elastic Stockings to Maintain Reduction in Leg Volume after Mechanical Lymph Drainage

The objective of the present study was to evaluate the effect of elastic compression stockings on volumetric variations of lymphedematous limbs between mechanical lymph drainage sessions. Eleven patients with Grade II leg lymphedema, regardless of etiology, were evaluated in a randomized clinical trial. The ages ranged from 47 to 83 years old with a mean of 62.4 years. Participants were submitted to mechanical lymph drainage (RAGodoy) associated with adjusted and unadjusted knee-high elastic compression stockings (20/30 Venosan). The effect of these stockings on the maintenance of volumetric reductions between sessions of lymph drainage was assessed. In all, 33 evaluations were carried out, 18 of patients using well-adjusted stockings and 15 with badly-adjusted stockings. The differences in volumes were significant (unpaired t-test; P-value < 0.0001). Adjusting the compression provided by elastic stockings according to the size of the leg has a synergistic effect in reducing volume during mechanical lymph drainage

Priming in lettuce seeds : Condicionamento osmótico em sementes de alface

One of the methods used to promote the rapid and uniform germination of vegetable seeds is the use of osmotic conditioning. The technique aims to regulate the osmotic potential of the solution, thus restricting the entry of water into the seeds without them germinating during the process. This work evaluated the germination of lettuce seeds of Grand Rapids, Everglades, and Verônica cultivars, submitted to osmotic conditioning. The seeds were immersed in polyethylene glycol solutions (PEG 6000) at potentials of 0.0; -0.6; -0.8, and -1.0 MPa, and remained in imbibition for 14, 24, and 48 h. After this period, the seeds were germinated on paper, moistened with water at 2.5 times their mass, and kept at 20ºC for seven days. The experimental design used was completely randomized, in a factorial scheme with three cultivars, four osmotic potentials, and three temperatures, with four replications of 25 seeds each. The germination percentage, the germination speed index, the mean speed of germination, and the mean germination time were evaluated. Data were subjected to analysis of residual normality by the Shapiro-Wilk test and the homogeneity of variances using the Bartlett test and submitted to analysis of variance. Data from osmotic potential and time of imbibition were submitted to polynomial regression. The treatment means were compared at 5% probability. Seeds of ‘Grand Rapids’ and ‘Everglades’ showed positive effects after osmoconditiong treatments

SOCIODEMOGRAPHIC AND BEHAVIORAL FACTORS ASSOCIATED WITH BODY ADIPOSITY IN ADULTS

A obesidade é uma condição multicausal, caracterizada pelo excesso de peso, resultante do desequilíbrio do balanço energético. O objetivo deste estudo foi avaliar os fatores sociodemográficos e comportamentais associados ao elevado percentual de gordura corporal (GC) e à obesidade abdominal (OA), numa população adulta, servidores da Universidade Federal do Tocantins. Participaram do estudo 226 servidores, os quais foram submetidos a avaliação antropométrica, e responderam a um questionário socioeconômico e comportamental. As prevalências de sobrepeso e obesidade foram 33,09% e 16,18%, respectivamente, para mulheres e 36,67% e 16,67%, para homens, a OA esteve relacionada à idade em ambos os sexos, a GC diretamente relacionada à idade e escolaridade nos homens. 67,78% dos homens consumiam bebida alcoólica; 46,32% das mulheres eram sedentárias. Nos homens, o tabagismo esteve associado ao aumento do perímetro da cintura (PC). As prevalências de sedentarismo e consumo de bebidas alcoólicas foram consideradas elevadas em relação ao preconizado na literatura. Os percentuais de indivíduos em sobrepeso e obesidade foram maiores entre homens. O PC aumentou conforme aumento da idade nas mulheres. A GC aumentou conforme o grau de escolaridade nos homens. O tabagismo associou-se ao PC elevado nos homens, a prática de atividade física não demostrou-se fator de proteção.Obesity is a multi-causal condition, characterized by excess weight, resulting from energy balance imbalance. The objectives of this study was to evaluate the sociodemographic and behavioral factors associated with the high percentage of body fat (CG) and abdominal obesity (OA) in an adult population, servants of the Federal University of Tocantins. 226 servants participated in the study, who underwent anthropometric assessment and answered a socioeconomic and behavioral questionnaire. The prevalence of overweight and obesity was 33.09% and 16.18%, respectively, for women and 36.67% and 16.67%, for men, AO was related to age in both sexes, to BF directly related to age and schooling in men. 67.78% of men consumed alcoholic beverages; 46.32% of the women were sedentary. In men, smoking was associated with increased waist circumference (WC). The prevalence of sedentarism and consumption of alcoholic beverages were considered high in relation to that recommended in the literature. The percentages of individuals in overweight and obesity were higher among men. WC increased with increasing age in women. The BF increased according to the level of schooling in men. Smoking was associated with elevated WC in men; physical activity practice was not a protective factor

E A EDUCAÇÃO FÍSICA? NARRATIVAS DE PROFESSORES-PESQUISADORES SOBRE AS AULAS REMOTAS EM INSTITUTOS FEDERAIS

The implementation of remote learning as a result of the COVID-19 pandemic has become a new and complex reality for school communities. In this context, this study analysed the experiences of Physical Education teachers- researcher with remote learning at Federal Institutes. Based on the Freirean perspective and social learning theory, the study was based on a qualitative and collaborative approach, through autobiographical narrative method. Eight teachers-researchers participated in the study. Data were generated from narratives produced by teachers-researchers and were analysed through constant comparisons. Four themes were identified as findings: new scenarios and challenges; peer collaboration; connecting with students; and rethinking Physical Education lessons. In conclusion, teachers’ experiences involved transformative responses to the limits faced in remote learning, including listening to students’ voices and forming a study group for collaborative learning. La implementación de la enseñanza remota como resultado de la pandemia COVID-19 se ha convertido en una realidad nueva y compleja para las comunidades escolares. En este contexto, el objetivo de este trabajo ha sido analizar las experiencias de profesores-investigadores de Educación Física con la enseñanza remota en Institutos Federales. Con base en la perspectiva freireana y en la teoría social del aprendizaje, la investigación se desarrolló con enfoque cualitativo y colaborativo, a través del método de la narrativa autobiográfica. Ocho profesores-investigadores participaron del estudio. Los datos se generaron a partir de narrativas elaboradas por los profesores-investigadores y se analizaron mediante comparaciones constantes. Como resultados, se identificaron cuatro temas: nuevos escenarios y desafíos; aproximación y colaboración entre pares; conectando con los estudiantes; y repensando las clases de Educación Física. En conclusión, las experiencias docentes implicaron respuestas transformadoras frente a los límites encontrados en la enseñanza remota, incluyendo escuchar a los estudiantes y formar un grupo de estudios y aprendizaje colaborativo. A implementação do ensino remoto em decorrência da pandemia da covid-19 se configurou como uma nova e complexa realidade para as comunidades escolares. Neste contexto, o objetivo deste trabalho foi analisar as experiências de professores-pesquisadores de Educação Física com o ensino remoto em Institutos Federais. Embasada na perspectiva freireana e na teoria social da aprendizagem, a pesquisa foi desenvolvida com abordagem qualitativa e colaborativa através do método da narrativa autobiográfica. Oito professores-pesquisadores participaram do estudo. Os dados foram gerados a partir de narrativas produzidas pelos professores-pesquisadores e foram analisados através de comparações constantes. Como resultados, quatro temas foram identificados: novos cenários e desafios; aproximação e colaboração entre pares; conectando com os estudantes; e repensando as aulas de Educação Física. Em conclusão, as experiências docentes envolveram respostas transformadoras frente aos limites encontrados no ensino remoto, incluindo a escuta dos estudantes e a formação de um grupo de estudos e aprendizagem colaborativa.  

Development and method validation for determination of 128 pesticides in bananas by modified QuEChERS and UHPLC–MS/MS analysis

AbstractA multiresidue method for the quantification of 128 pesticides in banana is described. It involves the application of a modified QuEChERS procedure followed by UHPLC–MS/MS (Ultra High Performance Liquid Chromatography coupled to Tandem Mass Spectrometry) analysis. The method was validated according to the European Union SANCO/12495/2011 guidelines and Brazilian Manual of Analytical Quality Assurance. The validation levels were 10.0; 25.0; 50.0 and 100 μg kg−1. Acceptable values were obtained for the following parameters: linearity, limit of detection – LOD (5.00 μg kg−1) and limit of quantification – LOQ (10.0 μg kg−1), except for fenamiphos and mevinphos (LOD = 7.5 μg kg−1 and LOQ = 25 μg kg−1), trueness (for the levels: 10.0, 25.0, 50.0 and 100 μg kg−1 the recovery assays values were between 70 and 120%) except for methamidophos at 10 μg kg−1 level (67.5%), intermediate precision (<20.0%) and measurement uncertainty tests (<50.0%). These results demonstrate the applicability of this method in the routine practice by the laboratories of Ministry of Agriculture, Livestock and Food Supply of Brazil that attend the National Control Plan for Residues and Contaminants (PNCRC)