Real-time selective sequencing using nanopore technology

The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set

AS AVENTURAS DE DOG MENDONÇA E PIZZA BOY: UMA LEITURA

Na trilogia As Aventuras de Dog Mendonça e Pizza Boy (2010; 2011; 2013) e sua prequela (2012), Filipe Melo e Juan Cavia povoam Portugal, e em particular Lisboa, com vários corpos fantásticos: gárgulas, vampiros, lobisomens, zombies, demónios e outros seres habitam o universo lusitano naquela que é uma obra com claras referências cinematográficas aos mestres do cinema de horror e outros. Ao mesmo tempo, esta homenagem apresenta também uma reflexão sobre o corpo fantástico, a monstruosidade e o Outro. Centrando-se essencialmente nas figuras sobrenaturais desta obra, este ensaio aborda os corpos fantásticos presentes nesta BD, em particular o caso de Dog Mendonça, o lobisomem, e de Pazuul, o demónio em corpo de criança. Desta forma, o presente estudo tem um duplo objetivo: por um lado, explorar as influências fantásticas de As Aventuras de Dog Mendonça e Pizza Boy e, por outro, estabelecer uma leitura do corpo fantástico a partir da análise de duas personagens que circulam entre dois mundos numa mesma cidade, o mundo sobrenatural e o mundo humano

Identification of novel imidazo[1,2-a]pyridine inhibitors targeting M. tuberculosis QcrB.

Mycobacterium tuberculosis is a major human pathogen and the causative agent for the pulmonary disease, tuberculosis (TB). Current treatment programs to combat TB are under threat due to the emergence of multi-drug and extensively-drug resistant TB. Through the use of high throughput whole cell screening of an extensive compound library a number of imidazo[1,2-a]pyridine (IP) compounds were obtained as potent lead molecules active against M. tuberculosis and Mycobacterium bovis BCG. The IP inhibitors (1–4) demonstrated minimum inhibitory concentrations (MICs) in the range of 0.03 to 5 µM against a panel of M. tuberculosis strains. M. bovis BCG spontaneous resistant mutants were generated against IP 1, 3, and 4 at 5× MIC and subsequent whole genome sequencing identified a single nucleotide polymorphism 937ACC>937GCC (T313A) in qcrB, which encodes the b subunit of the electron transport ubiquinol cytochrome C reductase. This mutation also conferred cross-resistance against IP 1, 3 and 4 demonstrating a common target. Gene dosage experiments confirmed M. bovis BCG QcrB as the target where over-expression in M. bovis BCG led to an increase in MIC from 0.5 to >8 µM for IP 3. An acute murine model of TB infection established bacteriostatic activity of the IP series, which await further detailed characterization

Nevrological manifestations in patiens with West Nile fever

Гарячка Західного Нілу (ГЗН) є найпоширенішим зооантропонозним трансмісивним «комариним» захворюванням у групі природно осередкових інфекцій. Вірус ГЗН належить до роду Flavivirus родини Flaviviridae, поширений на усіх континентах за винятком Антарктиди. В Європі основним видом комарів, які передають вірус ГЗН людям, є Culex pipiens. Інфікування описане також при вертикальній передачі від матері до дитини та парентеральним шляхом. За філогенетичними властивостями вірусу розрізняють кілька генетичних груп – генотипів, деякі з яких містять підгрупи, що обумовлює нерівномірний територіальний розподіл та тяжкість клінічної маніфестації ГЗН