Apolipoprotein D synthesis progressively increases in frontal cortex during human lifespan

Apolipoprotein D (apo D) is a lipocalin present in the nervous system that may be related to processes of reinnervation, regeneration and neuronal cell protection. In the other way, apo D expression has been correlated, in some brain regions, with normal aging and neurodegenerative diseases. To elucidate the regional and cellular expression of apo D in normal human brain during aging, we performed a detailed and extensive study in samples of post-mortem human cerebral cortices. To achieve this study, slot blot techniques, for protein and mRNA, as well as immunohistochemistry and hybridohistochemistry methods were used. A positive correlation for apo D expression with aging was found; furthermore, mRNA levels, as well as the protein ones, were higher in the white than in the grey matter. Immunohistochemistry and non-isotopic HIS showed that apo D is synthesized in both neurons and glial cells. Apo D expression is notorious in oligodendrocytes but with aging the number of neurons that synthesize apo D is increased. Our results indicate that apo D could play a fundamental role in central nervous system aging and in the reduction of products derivated from lipid peroxidation. The increment in the expression of apo D with aging can be included in a global mechanism of cellular protection to prevent the deleterious effects caused by aging

Novel Exercise Hardware Requirements, Development, and Selection Process for Long-Duration Space Flight

Long-duration space flight poses many hazards to the health of the crew. Among those hazards is the physiological deconditioning of the musculoskeletal and cardiovascular systems due to prolonged exposure to microgravity. To combat the physical toll that exploration space flight may take on the crew, NASAs Human Research Program is charged with developing exercise protocols and hardware to maintain astronaut health and fitness during long-term missions. The goal of this effort is to preserve the physical capability of the crew to perform mission critical tasks in transit and during planetary surface operations. As NASA aims toward space travel outside of low-earth orbit (LEO), the constraints placed upon exercise equipment onboard the vehicle increase. Proposed vehicle architectures for transit to and from locations outside of LEO call for limits to equipment volume, mass, and power consumption. While NASA has made great strides in providing for the physical welfare of the crew, the equipment currently used onboard ISS is too large, too massive, and too power hungry to consider for long-duration flight. The goal of the Advanced Exercise Concepts (AEC) project is to maintain the resistive and aerobic capabilities of the current, ISS suite of exercise equipment, while making reductions in size, mass, and power consumption in order to make the equipment suitable for long-duration missions

Evolution of the Aging Brain Transcriptome and Synaptic Regulation

Alzheimer's disease and other neurodegenerative disorders of aging are characterized by clinical and pathological features that are relatively specific to humans. To obtain greater insight into how brain aging has evolved, we compared age-related gene expression changes in the cortex of humans, rhesus macaques, and mice on a genome-wide scale. A small subset of gene expression changes are conserved in all three species, including robust age-dependent upregulation of the neuroprotective gene apolipoprotein D (APOD) and downregulation of the synaptic cAMP signaling gene calcium/calmodulin-dependent protein kinase IV (CAMK4). However, analysis of gene ontology and cell type localization shows that humans and rhesus macaques have diverged from mice due to a dramatic increase in age-dependent repression of neuronal genes. Many of these age-regulated neuronal genes are associated with synaptic function. Notably, genes associated with GABA-ergic inhibitory function are robustly age-downregulated in humans but not in mice at the level of both mRNA and protein. Gene downregulation was not associated with overall neuronal or synaptic loss. Thus, repression of neuronal gene expression is a prominent and recently evolved feature of brain aging in humans and rhesus macaques that may alter neural networks and contribute to age-related cognitive changes

Distinct Transcriptome Expression of the Temporal Cortex of the Primate Microcebus murinus during Brain Aging versus Alzheimer's Disease-Like Pathology

Aging is the primary risk factor of neurodegenerative disorders such as Alzheimer's disease (AD). However, the molecular events occurring during brain aging are extremely complex and still largely unknown. For a better understanding of these age-associated modifications, animal models as close as possible to humans are needed. We thus analyzed the transcriptome of the temporal cortex of the primate Microcebus murinus using human oligonucleotide microarrays (Affymetrix). Gene expression profiles were assessed in the temporal cortex of 6 young adults, 10 healthy old animals and 2 old, “AD-like” animals that presented ß-amyloid plaques and cortical atrophy, which are pathognomonic signs of AD in humans. Gene expression data of the 14,911 genes that were detected in at least 3 samples were analyzed. By SAM (significance analysis of microarrays), we identified 47 genes that discriminated young from healthy old and “AD-like” animals. These findings were confirmed by principal component analysis (PCA). ANOVA of the expression data from the three groups identified 695 genes (including the 47 genes previously identified by SAM and PCA) with significant changes of expression in old and “AD-like” in comparison to young animals. About one third of these genes showed similar changes of expression in healthy aging and in “AD-like” animals, whereas more than two thirds showed opposite changes in these two groups in comparison to young animals. Hierarchical clustering analysis of the 695 markers indicated that each group had distinct expression profiles which characterized each group, especially the “AD-like” group. Functional categorization showed that most of the genes that were up-regulated in healthy old animals and down-regulated in “AD-like” animals belonged to metabolic pathways, particularly protein synthesis. These data suggest the existence of compensatory mechanisms during physiological brain aging that disappear in “AD-like” animals. These results open the way to new exploration of physiological and “AD-like” aging in primates

The positive impact of red palm oil in school meals on vitamin A status: study in Burkina Faso

BACKGROUND: Vitamin A (VA) deficiency is widespread in sub-Saharan Africa and school-age children are a vulnerable group. In Burkina Faso, the production and consumption of red palm oil (RPO) is being promoted as a food supplement for VA. The objective of the study was to assess the impact on serum retinol of adding RPO to school lunch in two test zones of Burkina Faso. METHODS: Over one school year, 15 ml RPO was added to individual meals 3 times a week in selected primary schools in two sites. Serum retinol was measured with HPLC at baseline and exactly 12 months later to take account of seasonality. A simple pre-post test design was used in the Kaya area (north-central Burkina), where 239 pupils from 15 intervention schools were randomly selected for the evaluation. In Bogandé (eastern Burkina), 24 schools were randomised for the controlled intervention trial: 8 negative controls (G1) with only the regular school lunch; 8 positive controls (G2) where the pupils received a single VA capsule (60 mg) at the end of the school year; and 8 schools with RPO through the school year (G3). A random sample of 128 pupils in each school group took part in the evaluation. RESULTS: In Kaya, serum retinol went from 0.77 ± 0.37 μmol/L at baseline to 1.07 ± 0.40 μmol/L one year later (p < 0.001). The rate of low serum retinol (<0.7 μmol/L) declined from 47.2% to 13.1%. In Bogandé, serum retinol increased significantly (p < 0.001) only in the capsule and RPO groups, going from 0.77 ± 0.28 to 0.98 ± 0.33 μmol/L in the former, and from 0.82 ± 0.3 to 0.98 ± 0.33 μmol/L in the latter. The rate of low serum retinol went from 46.1 to 17.1% in the VA capsule group and from 40.4% to 14.9% in the RPO group. VA-deficient children benefited the most from the capsule or RPO. Female sex, age and height-for-age were positively associated with the response to VA capsules or RPO. CONCLUSION: RPO given regularly in small amounts appears highly effective in the reduction of VA deficiency. RPO deserves more attention as a food supplement for VA and as a potential source of rural income in Sahelian countries

New insights into the genetic etiology of Alzheimer's disease and related dementias

Characterization of the genetic landscape of Alzheimer's disease (AD) and related dementias (ADD) provides a unique opportunity for a better understanding of the associated pathophysiological processes. We performed a two-stage genome-wide association study totaling 111,326 clinically diagnosed/'proxy' AD cases and 677,663 controls. We found 75 risk loci, of which 42 were new at the time of analysis. Pathway enrichment analyses confirmed the involvement of amyloid/tau pathways and highlighted microglia implication. Gene prioritization in the new loci identified 31 genes that were suggestive of new genetically associated processes, including the tumor necrosis factor alpha pathway through the linear ubiquitin chain assembly complex. We also built a new genetic risk score associated with the risk of future AD/dementia or progression from mild cognitive impairment to AD/dementia. The improvement in prediction led to a 1.6- to 1.9-fold increase in AD risk from the lowest to the highest decile, in addition to effects of age and the APOE ε4 allele

Department of Animal Sciences research and reviews: beef and sheep

Relationship of a PCR-SSCP at the Bovine calpastatin locus with calpastatin activity and meat tenderness / H. Y. Chung, M. E. Davis, H. C. Hines, and D. M. Wulf -- Effects of calpain proteolysis and calpain genotypes on meat tenderness of angus bulls / H. Y. Chung, M. E. Davis, H. C. Hines, and D. M. Wulf -- Identification of genetic markers for growth and carcass traits in beef cattle / W. Ge, M. E. Davis, H. C. Hines, and K. M. Irvin -- Relationships of polymorphisms in the bovine leptin gene with differences in beef carcass traits / K. Tessanne, H. C. Hines, and M. E. Davis -- Effects of rate of gain during periods of restricted intake on performance and carcass characteristics in steers fed to achieve step-wise increases in rate of gain / J. E. Rossi and S. C. Loerch -- Effects of feeding regimen and days fed on performance and carcass characteristics of feedlot steers / J. E. Rossi, S. C. Loerch, S. J. Moeller, and J. P. Schoonmaker -- Effect of an accelerated finishing program on performance and carcass characteristics of early weaned bulls and steers / J. P. Schoonmaker, S. C. Loerch, F. L. Fluharty, T. B. Turner, S. J. Moeller and J. E. Rossi -- Yeast-mineral mixes and beef-cattle performance in fescue-based grazing systems: preliminary report / S. Boyles, W. Shriver, and D. Kobs -- Forage and animal evaluation of heifers at Indian Lake Hydrologic Unit CRP stocker grazing demonstration / S. L. Boyles, B. W. Stoll, and T. L. Dobbels -- Beef quality is every cattleman's business: education program / J. Yates and S. Boyles -- Effects of pelleted alfalfa and whole-shelled corn combinations on lamb growth and carcass characteristics / F. L. Fluharty -- Effects of feeding pelleted, ensiled, or a combination of pelleted and ensiled alfalfa on lamb growth and carcass characteristics / F. L. Fluharty, G. D. Lowe, and D. D. Clevenger -- Effects of corn silage vs. alfalfa haylage on lamb growth and carcass characteristics in forage-based finishing systems / F. L. Fluharty, G. D. Lowe, and D. D. Clevenger -- Effects of feed-delivery system and corn processing on lamb growth and carcass characteristics / F. L. Fluharty, G. D. Lowe, and D. D. Clevenger -- Effects of pen floor type and bedding on lamb growth and carcass characteristics / F. L. Fluharty, G. D. Lowe, and D. D. Clevenger -- A PCR-SSCP polymorphism detected in the 5' flanking region of the ovine IGF-I gene / A. Yilmaz, M. E. Davis, and H. C. Hine