4,348 research outputs found
Achieving a highâdensity oleaginous yeast culture: Comparison of four processing strategies using <i>Metschnikowia pulcherrima</i>
Microbial lipids have the potential to displace terrestrial oils for fuel, value chemical, and food production, curbing the growth in tropical oil plantations and helping to reduce deforestation. However, commercialization remains elusive partly due to the lack of suitably robust organisms and their low lipid productivity. Extremely high cell densities in oleaginous cultures are needed to increase reaction rates, reduce reactor volume, and facilitate downstream processing. In this investigation, the oleaginous yeast Metschnikowia pulcherrima, a known antimicrobial producer, was cultured using four different processing strategies to achieve high cell densities and gain suitable lipid productivity. In batch mode, the yeast demonstrated lipid contents more than 40%â(w/w) under high osmotic pressure. In fedâbatch mode, however, highâlipid titers were prevented through inhibition above 70.0âgâLâ1 yeast biomass. Highly promising were a semiâcontinuous and continuous mode with cell recycle where cell densities of up to 122.6âgâLâ1 and maximum lipid production rates of 0.37âgâLâ1âhâ1 (daily average), a nearly twoâfold increase from the batch, were achieved. The findings demonstrate the importance of considering multiple fermentation modes to achieve highâdensity oleaginous yeast cultures generally and indicate the limitations of processing these organisms under the extreme conditions necessary for economic lipid production.This project has received funding from the European Unionâs Horizon 2020 research and innovation programme under the Marie SkĆodowska-Curie grant agreement No 665992
Arbitrary real-time three-dimensional corporal object sensing and reconstruction scheme
A real-time three-dimensional (3D) object sensing and reconstruction scheme is presented that can be applied on any arbitrary corporeal shape. Operation is demonstrated on several calibrated objects. The system uses curvature sensors based upon in-line fiber Bragg gratings encapsulated in a low-temperature curing synthetic silicone. New methods to quantitatively evaluate the performance of a 3D object-sensing scheme are developed and appraised. It is shown that the sensing scheme yields a volumetric error of 1% to 9%, depending on the object
Constraints on relaxation rates for N-level quantum systems
We study the constraints imposed on the population and phase relaxation rates
by the physical requirement of completely positive evolution for open N-level
systems. The Lindblad operators that govern the evolution of the system are
expressed in terms of observable relaxation rates, explicit formulas for the
decoherence rates due to population relaxation are derived, and it is shown
that there are additional, non-trivial constraints on the pure dephasing rates
for N>2. Explicit experimentally testable inequality constraints for the
decoherence rates are derived for three and four-level systems, and the
implications of the results are discussed for generic ladder-, Lambda- and
V-systems, and transitions between degenerate energy levels.Comment: 10 pages, RevTeX, 4 figures (eps/pdf
Multiscale imaging of basal cell dynamics in the functionally mature mammary gland
The mammary epithelium is indispensable for the continued survival of more than 5,000 mammalian species. For some, the volume of milk ejected in a single day exceeds their entire blood volume. Here, we unveil the spatiotemporal properties of physiological signals that orchestrate the ejection of milk from alveolar units and its passage along the mammary ductal network. Using quantitative, multidimensional imaging of mammary cell ensembles from GCaMP6 transgenic mice, we reveal how stimulus evoked Ca oscillations couple to contractions in basal epithelial cells. Moreover, we show that Ca-dependent contractions generate the requisite force to physically deform the innermost layer of luminal cells, compelling them to discharge the fluid that they produced and housed. Through the collective action of thousands of these biological positive-displacement pumps, each linked to a contractile ductal network, milk begins its passage toward the dependent neonate, seconds after the command
The Imprint of Gravitational Waves in Models Dominated by a Dynamical Cosmic Scalar Field
An alternative to the standard cold dark matter model has been recently
proposed in which a significant fraction of the energy density of the universe
is due to a dynamical scalar field () whose effective equation-of-state
differs from that of matter, radiation or cosmological constant (). In
this paper, we determine how the Q-component modifies the primordial inflation
gravitational wave (tensor metric) contribution to the cosmic microwave
background anisotropy and, thereby, one of the key tests of inflation.Comment: 15 pages, 14 figures, revtex, submitted to Phys. Rev.
Hiding in Plain Sight-Ancient Chinese Anatomy
For thousands of years, scientists have studied human anatomy by dissecting bodies. Our knowledge of their findings is limited, however, both by the subsequent loss of many of the oldest texts, and by a tendency towards a Eurocentric perspective in medicine. As a discipline, anatomy tends to be much more familiar with ancient Greek texts than with those from India, China or Persia. Here we show that the Mawangdui medical texts, entombed in the Mawangdui burial site in Changsha, China 168BCE, are the oldest surviving anatomical atlas in the world. These medical texts both predate and inform the later acupuncture texts which have been the foundation for acupuncture practice in the subsequent two millennia. The skills necessary to interpret them are diverse, requiring the researcher firstly to read the original Chinese, and secondly to perform the anatomical investigations that allow a re-viewing of the structures that the texts refer to. Acupuncture meridians are considered to be esoteric in nature, but these texts are clearly descriptions of the physical body. As such, they represent a previously hidden chapter in the history of anatomy, and a new perspective on acupuncture
S.cerevisiae Complex Function Prediction with Modular Multi-Relational Framework
Proceeding of: 23rd International Conference on Industrial Engineering and Other Applications of Applied Intelligent Systems, IEA/AIE 2010, CĂłrdoba, Spain, June 1-4, 2010Determining the functions of genes is essential for understanding how the metabolisms work, and for trying to solve their malfunctions. Genes usually work in groups rather than isolated, so functions should be assigned to gene groups and not to individual genes. Moreover, the genetic knowledge has many relations and is very frequently changeable. Thus, a propositional ad-hoc approach is not appropriate to deal with the gene group function prediction domain. We propose the Modular Multi-Relational Framework (MMRF), which faces the problem from a relational and flexible point of view. The MMRF consists of several modules covering all involved domain tasks (grouping, representing and learning using computational prediction techniques). A specific application is described, including a relational representation language, where each module of MMRF is individually instantiated and refined for obtaining a prediction under specific given conditions.This research work has been supported by CICYT, TRA 2007-67374-C02-02 project and by the expert biological knowledge of the Structural Computational Biology Group in Spanish National Cancer Research Centre (CNIO). The authors would like to thank members of Tilde tool developer
group in K.U.Leuven for providing their help and many useful suggestions.Publicad
Modern optical astronomy: technology and impact of interferometry
The present `state of the art' and the path to future progress in high
spatial resolution imaging interferometry is reviewed. The review begins with a
treatment of the fundamentals of stellar optical interferometry, the origin,
properties, optical effects of turbulence in the Earth's atmosphere, the
passive methods that are applied on a single telescope to overcome atmospheric
image degradation such as speckle interferometry, and various other techniques.
These topics include differential speckle interferometry, speckle spectroscopy
and polarimetry, phase diversity, wavefront shearing interferometry,
phase-closure methods, dark speckle imaging, as well as the limitations imposed
by the detectors on the performance of speckle imaging. A brief account is
given of the technological innovation of adaptive-optics (AO) to compensate
such atmospheric effects on the image in real time. A major advancement
involves the transition from single-aperture to the dilute-aperture
interferometry using multiple telescopes. Therefore, the review deals with
recent developments involving ground-based, and space-based optical arrays.
Emphasis is placed on the problems specific to delay-lines, beam recombination,
polarization, dispersion, fringe-tracking, bootstrapping, coherencing and
cophasing, and recovery of the visibility functions. The role of AO in
enhancing visibilities is also discussed. The applications of interferometry,
such as imaging, astrometry, and nulling are described. The mathematical
intricacies of the various `post-detection' image-processing techniques are
examined critically. The review concludes with a discussion of the
astrophysical importance and the perspectives of interferometry.Comment: 65 pages LaTeX file including 23 figures. Reviews of Modern Physics,
2002, to appear in April issu
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