Double-clad fiber with a tapered end for confocal endomicroscopy

We present a double-clad fiber coupler (DCFC) for use in confocal endomicroscopy to reduce speckle contrast, increase signal collection while preserving optical sectioning. The DCFC is made by incorporating a double-clad tapered fiber (DCTF) to a fused-tapered DCFC for achromatic transmission (from 1265 nm to 1325 nm) of > 95% illumination light trough the single mode (SM) core and collection of > 40% diffuse light through inner cladding modes. Its potential for confocal endomicroscopy is demonstrated in a spectrally-encoded imaging setup which shows a 3 times reduction in speckle contrast as well as 5.5 × increase in signal collection compared to imaging with a SM fiber

The effect of public target on the public-private partnership (PPP) residential development

A growing importance of public-private partnership (PPP) in public housing projects has drawn much attention. This paper presents a theoretical analysis exploring the effect of the public target on the private’s optimal strategy in a PPP housing project. An option-based model is established to show that an increase in the proportion of public housing will delay the project development. It indicates that the government needs to consider the trade-off between the waiting time and the supply of public housing. On the other hand, due to the delay effect, the expected project value would rise because the private developer is willing to wait for a better environment in the presence of a rise in public housing. Both private and public sector can benefit from this accurate evaluation model and its implications

Fast and convenient delivery of fluidextracts liquorice through electrospun core-shell nanohybrids

Introduction: As an interdisciplinary field, drug delivery relies on the developments of modern science and technology. Correspondingly, how to upgrade the traditional dosage forms for a more efficacious, safer, and convenient drug delivery poses a continuous challenge to researchers.Methods, results and discussion: In this study, a proof-of-concept demonstration was conducted to convert a popular traditional liquid dosage form (a commercial oral compound solution prepared from an intermediate licorice fluidextract) into a solid dosage form. The oral commercial solution was successfully encapsulated into the core–shell nanohybrids, and the ethanol in the oral solution was removed. The SEM and TEM evaluations showed that the prepared nanofibers had linear morphologies without any discerned spindles or beads and an obvious core–shell nanostructure. The FTIR and XRD results verified that the active ingredients in the commercial solution were compatible with the polymeric matrices and were presented in the core section in an amorphous state. Three different types of methods were developed, and the fast dissolution of the electrospun core–shell nanofibers was verified.Conclusion: Coaxial electrospinning can act as a nano pharmaceutical technique to upgrade the traditional oral solution into fast-dissolving solid drug delivery films to retain the advantages of the liquid dosage forms and the solid dosage forms

Observed Changes of Koppen Climate Zones Based on High-Resolution Data Sets in the Qinghai-Tibet Plateau

Emerging and disappearing climate zones are frequently used to diagnose and project climate change. However, little attempt has been made to quantify shifts of climate zones in Qinghai-Tibet Plateau (QTP) based on the high-resolution data sets. Our results show that highland climate was decreased substantially during 1961–2011 and were mainly replaced by boreal climate. We also found that the mean elevation of boreal and highland climate continues to rise, with obvious longitudinal geographical characteristics over the study period. Furthermore, we found that the climate spaces (a climate space defined as the volume of 10°C × 500 mm here) of both boreal and highland climate types tend to be warm and humid ones, which may provide more suitable climate conditions for species to maintain and promote diversity. Characterization of changes in QTP climate types deepens our understanding of regional climate and its biological impacts.Emerging and disappearing climate zones are frequently used to diagnose and project climate change. However, little attempt has been made to quantify shifts of climate zones in Qinghai-Tibet Plateau (QTP) based on the high-resolution data sets. Our results show that highland climate was decreased substantially during 1961-2011 and were mainly replaced by boreal climate. We also found that the mean elevation of boreal and highland climate continues to rise, with obvious longitudinal geographical characteristics over the study period. Furthermore, we found that the climate spaces (a climate space defined as the volume of 10 degrees C x 500 mm here) of both boreal and highland climate types tend to be warm and humid ones, which may provide more suitable climate conditions for species to maintain and promote diversity. Characterization of changes in QTP climate types deepens our understanding of regional climate and its biological impacts. Plain Language Summary Climate classification is the key to simplifying complex climate and helps to deepen the understanding of regional climate change. Based on the high-resolution data set (LZ0025), the sharp climatic gradient features and their potential biological impact on Qinghai-Tibet Plateau (QTP) was quantified. With the temperature increase, the spatial distribution of highland tundra climate was gradually replaced by boreal climate. More importantly, the contraction of highland climate and the expansion of boreal climate has obvious elevation characteristics. In addition, climate spaces of highland and boreal climate types tend to warm and humid ones, which may provide more climatic niches for different species and contribute to regional biodiversity.Peer reviewe

Nonlinear optics via double dark resonances

Double dark resonances originate from a coherent perturbation of a system displaying electromagnetically induced transparency. We experimentally show and theoretically confirm that this leads to the possibility of extremely sharp resonances prevailing even in the presence of considerable Doppler broadening. A gas of 87Rb atoms is subjected to a strong drive laser and a weak probe laser and a radio frequency field, where the magnetic coupling between the Zeeman levels leads to nonlinear generation of a comb of sidebands.Comment: 6 pages, 9 figure

From a Spatial Structure Perspective : Spatial-Temporal Variation of Climate Redistribution of China Based on the Köppen–Geiger Classification

https://doi.org/10.1029/2022GL099319Shifting climate zones are widely used to diagnose and predict regional climate change. However, few attempts have been made to measure the spatial redistribution of these climate zones from a spatial structure perspective. We investigated changes in spatial structure of Köppen climate landscape in China between 1963 and 2098 with a landscape aggregation index. Our results reveal an apparent signal from fragmentation to aggregation, accompanied by the intensification of areal dispersion between cold and warm climate types. Our attribution analysis indicates that anthropogenic forcings have a larger influence on changes of spatial structure than natural variation. We also found that topographical heterogeneity is likely to contribute to the regional spatial fragmentation, especially in the Qinghai-Tibet Plateau. However, we also found that the spatial fragmentation will be weakened around the mid-2040s. We argue that biodiversity is likely to be mediated by spatial structure of future climate landscapes in China.Peer reviewe

Comprehensive volumetric confocal microscopy with adaptive focusing

Comprehensive microscopy of distal esophagus could greatly improve the screening and surveillance of esophageal diseases such as Barrett’s esophagus by providing histomorphologic information over the entire region at risk. Spectrally encoded confocal microscopy (SECM) is a high-speed reflectance confocal microscopy technology that can be configured to image the entire distal esophagus by helically scanning the beam using optics within a balloon-centering probe. It is challenging to image the human esophagus in vivo with balloon-based SECM, however, because patient motion and anatomic tissue surface irregularities decenter the optics, making it difficult to keep the focus at a predetermined location within the tissue as the beam is scanned. In this paper, we present a SECM probe equipped with an adaptive focusing mechanism that can compensate for tissue surface irregularity and dynamic focal variation. A tilted arrangement of the objective lens is employed in the SECM probe to provide feedback signals to an adaptive focusing mechanism. The tilted configuration also allows the probe to obtain reflectance confocal data from multiple depth levels, enabling the acquisition of three-dimensional volumetric data during a single scan of the probe. A tissue phantom with a surface area of 12.6 cm2 was imaged using the new SECM probe, and 8 large-area reflectance confocal microscopy images were acquired over the depth range of 56 μm in 20 minutes. Large-area SECM images of excised swine small intestine tissue were also acquired, enabling the visualization of villous architecture, epithelium, and lamina propria. The adaptive focusing mechanism was demonstrated to enable acquisition of in-focus images even when the probe was not centered and the tissue surface was irregular

Spatial Aggregation of Global Dry and Wet Patterns Based on the Standard Precipitation Index

Quantifying the spatial integrity and patterns of dry/wet events over land is essential to understand how the local hydrological regime responds to environmental changes. Spatial aggregation changes in dry and wet areas over land have not been studied extensively. Based on a patch-mosaic landscape model, we analyzed spatial aggregation changes at two levels corresponding to landscape design during 1949 and 2018. At the landscape level, the global aggregation degree increased initially and then weakened around 2006. However, the spatial aggregation process between dry and wet patterns was inconsistent. For the dry pattern, spatial aggregation was mainly caused by area decline induced decreases in the patch number. For the wet pattern, spatial aggregation was caused by area enlargement induced decreases in the patch number. At the class level, with increases in the dry/wet magnitude, the correlation between the affected area and aggregation strengthened. Our results provide new insights to understand the spatial processes and future trends of dry/wet patterns over land. We argue that future vulnerability of agriculture and ecosystems to drought is likely to be further mediated by the changes in drought patterns' spatial structure.Peer reviewe

GluN2A NMDA Receptor Enhancement Improves Brain Oscillations, Synchrony, and Cognitive Functions in Dravet Syndrome and Alzheimer's Disease Models.

NMDA receptors (NMDARs) play subunit-specific roles in synaptic function and are implicated in neuropsychiatric and neurodegenerative disorders. However, the in vivo consequences and therapeutic potential of pharmacologically enhancing NMDAR function via allosteric modulation are largely unknown. We examine the in vivo effects of GNE-0723, a positive allosteric modulator of GluN2A-subunit-containing NMDARs, on brain network and cognitive functions in mouse models of Dravet syndrome (DS) and Alzheimer's disease (AD). GNE-0723 use dependently potentiates synaptic NMDA receptor currents and reduces brain oscillation power with a predominant effect on low-frequency (12-20 Hz) oscillations. Interestingly, DS and AD mouse models display aberrant low-frequency oscillatory power that is tightly correlated with network hypersynchrony. GNE-0723 treatment reduces aberrant low-frequency oscillations and epileptiform discharges and improves cognitive functions in DS and AD mouse models. GluN2A-subunit-containing NMDAR enhancers may have therapeutic benefits in brain disorders with network hypersynchrony and cognitive impairments