Highway Bridge Traffic Loading

In this chapter, traditional approaches and recent advances in highway bridge traffic loading are described, which are of great significance for structural safety assessment of bridges. Indeed, it is widely accepted that consideration of site-specific traffic features can enable significant savings in maintenance operations. While short spans are governed by free-flowing traffic plus an allowance for the dynamic effects, long spans are governed by congested conditions. For the former, a promising research trend is the investigation of the dynamic vehicle-bridge interaction, which is shown to lead to dynamic effects much lower than previously thought. For the latter, advances in traffic flow modelling enable the simulation of realistic congestion patterns based on widely available free-flowing traffic data, thus partially overcoming a long-standing shortage of congestion data. Here, emphasis is given to the promising application of traffic microsimulation to long-span bridge loading, combined with a probabilistic approach based on the extreme value theory, to compute site-specific characteristic loading values

A Bandwidth Reservation Mechanism for AXI-Based Hardware Accelerators on FPGAs

Hardware platforms for real-time embedded systems are evolving towards heterogeneous architectures comprising different types of processing cores and dedicated hardware accelerators, which can be implemented on silicon or dynamically deployed on FPGA fabric. Such accelerators typically access a shared memory to exchange a significant amount of data with other processing elements. Existing COTS solutions focus on maximizing the overall throughput of the system, rather than guaranteeing the timing constraints of individual hardware accelerators. This paper presents the AXI budgeting unit (ABU), a hardware-based solution to implement a bandwidth reservation mechanism on top of the AMBA AXI standard infrastructure for hardware accelerators deployed on FPGAs. An accurate and tractable model, as well as the corresponding analysis, are also proposed to bound the response time of hardware accelerators in the presence of ABUs, in order to verify whether they can complete before their deadlines. Finally, a set of experiments are reported to evaluate the proposed approach on a state-of-the-art platform, namely the Zynq-7020 by Xilinx. The resource consumption of the ABU has been quantified to be less than 1% of the total FPGA resources of the Zynq-7020

Presence of oxytocin, vasopressin and atrial natriuretic peptide and their modification in rat hypothalamic paraventricular nucleus during resistance training.

Many studies have demonstrated the physiological effects of oxytocin (OT), atrial natriuretic peptide (ANP) and vasopressin (VP) in the homoeostasis of body fluids during physical exercise. However, a little information is available about the related immunohistochemical changes in hypothalamic magnocellular neurosecretory system during and after the training. The aim of the present work was to study the immunohistochemical changes in OT, ANP and VP levels in the hypothalamic paraventricular nucleus during and after resistance exercise protocol. Three groups of Wistar rats were trained by a rung ladder protocol for 15, 30 and 45 days, respectively; a fourth group was left to rest for 15 days after the training. Finally, four sedentary groups were used as controls. The results show that resistance training induces a significant reduction in the percentage of OT-positive neurons, compared with sedentary controls. In contrast, this protocol did not induce any change in VP levels, and ANP levels did not change significantly. However, VP increased after the resting period of 15 days. Our work shows that neurons of the paraventricular nucleus are involved in body fluid homoeostasis during and after resistance exercise. The functional significance of these changes in OT and VP levels, during and after the protocol, needs to be further investigated

Molecular analysis of sarcomeric and non-sarcomeric genes in patients with hypertrophic cardiomyopathy.

Background: Hypertrophic cardiomyopathy (HCM) is a common genetic heart disorder characterized by unexplained left ventricle hypertrophy associated with non-dilated ventricular chambers. Several genes encoding heart sarcomeric proteins have been associated to HCM, but a small proportion of HCM patients harbor alterations in other non-sarcomeric loci. The variable expression of HCM seems influenced by genetic modifier factors and new sequencing technologies are redefining the understanding of genotype–phenotype relationships, even if the interpretations of the numerous identified variants pose several challenges. Methods and results: We investigated 62 sarcomeric and non-sarcomeric genes in 41 HCM cases and in 3 HCM-related disorders patients. We employed an integrated approach that combines multiple tools for the prediction, annotation and visualization of functional variants. Genotype–phenotype correlations were carried out for inspecting the involvement of each gene in age onset and clinical variability of HCM. The 80% of the non-syndromic patients showed at least one rare non-synonymous variant (nsSNV) and among them, 58% carried alterations in sarcomeric loci, 14% in desmosomal and 7% in other non-sarcomeric ones without any sarcomere change. Statistical analyses revealed an inverse correlation between the number of nsSNVs and age at onset, and a relationship between the clinical variability and number and type of variants. Conclusions: Our results extend the mutational spectrum of HCM and contribute in defining the molecular pathogenesis and inheritance pattern(s) of this condition. Besides, we delineate a specific procedure for the identification of the most likely pathogenetic variants for a next generation sequencing approach embodied in a clinical context

Neutrino oscillations and large extra dimensions

Assuming that right-handed neutrinos exist and propagate in some large extra dimensions, we attempt to give a comprehensive description of the phenomenology of neutrino oscillations. A few alternative explanations of the atmospheric neutrino anomaly emerge, different from the standard nuMu --> nuTau or nuMu --> nuSterile interpretations. Constraints from nucleosynthesis and supernova 1987a are discussed. The constraints from SN1987a indicates a maximum radius of any extra dimension of about 1 Angstrom.Comment: 12 pages, 5 figures. Version 2: misprints fixed, ref.s added, conclusions modified: at least in minimal models the maximal radius of large extra dimensions where right-handed neutrinos can propagate must be smaller than an atom in order to satisfy bounds from the 1987 supernov

Prediction and visualization data for the interpretation of sarcomeric and non-sarcomeric DNA variants found in patients with hypertrophic cardiomyopathy

AbstractGenomic technologies are redefining the understanding of genotype–phenotype relationships and over the past decade, many bioinformatics algorithms have been developed to predict functional consequences of single nucleotide variants. This article presents the data from a comprehensive computational workflow adopted to assess the biomedical impact of the DNA variants resulting from the experimental study “Molecular analysis of sarcomeric and non-sarcomeric genes in patients with hypertrophic cardiomyopathy” (Bottillo et al., 2016) [1]. Several different independently methods were employed to predict the functional consequences of alleles that result in amino acid substitutions, to study the effect of some DNA variants over the splicing process and to investigate the impact of a sequence variant with respect to the evolutionary conservation

Interpreting the LSND anomaly: sterile neutrinos or CPT-violation or...?

We first study how sterile neutrinos can fit the 5sigma bar-nu_mu --> bar-nu_e LSND anomaly: 2+2 solutions are strongly disfavoured by solar and atmospheric data, while 3+1 solutions can still give a poor fit (for a specific range of oscillation parameters, to be tested by MiniBooNE). If instead MiniBoone will find no nu_mu --> nu_e signal, we will have a hint for CPT violation. Already now, unlike sterile neutrinos, CPT-violating neutrino masses can nicely accomodate all safe and unsafe data. We study how much CPT must be conserved according to atmospheric and K2K data and list which CPT-violating signals could be discovered by forthcoming solar and long-baseline experiments.Comment: 12 pages. Version 2: update about SNO. Version 3: addendum about KamLAND. Version 4: addendum at pages 11,12 discussing how much the recent WMAP data disfavour the 3+1 sterile solutio

MYC-driven epigenetic reprogramming favors the onset of tumorigenesis by inducing a stem cell-like state

Breast cancer consists of highly heterogeneous tumors, whose cell of origin and driver oncogenes are difficult to be uniquely defined. Here we report that MYC acts as tumor reprogramming factor in mammary epithelial cells by inducing an alternative epigenetic program, which triggers loss of cell identity and activation of oncogenic pathways. Overexpression of MYC induces transcriptional repression of lineage-specifying transcription factors, causing decommissioning of luminal-specific enhancers. MYC-driven dedifferentiation supports the onset of a stem cell-like state by inducing the activation of de novo enhancers, which drive the transcriptional activation of oncogenic pathways. Furthermore, we demonstrate that the MYC-driven epigenetic reprogramming favors the formation and maintenance of tumor-initiating cells endowed with metastatic capacity. This study supports the notion that MYC-driven tumor initiation relies on cell reprogramming, which is mediated by the activation of MYC-dependent oncogenic enhancers, thus establishing a therapeutic rational for treating basal-like breast cancers

Hybrid Approach to Atrial Fibrillation Ablation

Sequential surgical thoracoscopic and electrophysiological (EP) ablation is gaining popularity as a novel approach for the treatment of patients with stand-alone, persistent and long standing persistent atrial fibrillation (AF)

The primary cosmic ray composition between 10**15 and 10**16 eV from Extensive Air Showers electromagnetic and TeV muon data

The cosmic ray primary composition in the energy range between 10**15 and 10**16 eV, i.e., around the "knee" of the primary spectrum, has been studied through the combined measurements of the EAS-TOP air shower array (2005 m a.s.l., 10**5 m**2 collecting area) and the MACRO underground detector (963 m a.s.l., 3100 m w.e. of minimum rock overburden, 920 m**2 effective area) at the National Gran Sasso Laboratories. The used observables are the air shower size (Ne) measured by EAS-TOP and the muon number (Nmu) recorded by MACRO. The two detectors are separated on average by 1200 m of rock, and located at a respective zenith angle of about 30 degrees. The energy threshold at the surface for muons reaching the MACRO depth is approximately 1.3 TeV. Such muons are produced in the early stages of the shower development and in a kinematic region quite different from the one relevant for the usual Nmu-Ne studies. The measurement leads to a primary composition becoming heavier at the knee of the primary spectrum, the knee itself resulting from the steepening of the spectrum of a primary light component (p, He). The result confirms the ones reported from the observation of the low energy muons at the surface (typically in the GeV energy range), showing that the conclusions do not depend on the production region kinematics. Thus, the hadronic interaction model used (CORSIKA/QGSJET) provides consistent composition results from data related to secondaries produced in a rapidity region exceeding the central one. Such an evolution of the composition in the knee region supports the "standard" galactic acceleration/propagation models that imply rigidity dependent breaks of the different components, and therefore breaks occurring at lower energies in the spectra of the light nuclei.Comment: Submitted to Astroparticle Physic