54 research outputs found
Lateral gradients significantly enhance static magnetic field-induced inhibition of pain responses in mice-a double blind experimental study
Recent research demonstrated that exposure of mice to both inhomogeneous (3–477 mT) and
homogeneous (145 mT) static magnetic fields (SMF) generated an analgesic effect toward visceral
pain elicited by the intraperitoneal injection of 0.6% acetic acid. In the present work, we
investigated behavioral responses such as writhing, entry avoidance, and site preference with the
help of a specially designed cage that partially protruded into either the homogeneous (ho) or
inhomogeneous (inh) SMF. Aversive effects, cognitive recognition of analgesia, and social behavior
governed mice in their free locomotion between SMF and sham sides. The inhibition of pain
response (I) for the 0–5, 6–20, and 21–30 min periods following the challenge was calculated by
the formula I ¼ 100 (1 x/y) in %, where x and y represent the number of writhings in the SMF
and sham sides, respectively. In accordance with previous measurements, an analgesic effect was
induced in exposed mice (Iho ¼ 64%, P < 0.0002 and Iinh ¼ 62%, P < 0.002). No significant
difference was found in the site preference (SMFho, inh vs. sham) indicating that SMF is neither
aversive nor favorable. Comparison of writhings observed in the sham versus SMF side of the
cage revealed that SMF exposure resulted in significantly fewer writhings than sham (Iho ¼ 64%,
P < 0.004 and Iinh ¼ 81%, P < 0.03). Deeper statistical analysis clarified that the lateral SMF
gradient between SMF and sham sides could be responsible for most of the analgesic effect
(Iho ¼ 91%, P < 0.02 and Iinh ¼ 54%, P < 0.02)
Clinical evaluation of an allogeneic bone matrix containing viable osteogenic cells in patients undergoing one- and two-level posterolateral lumbar arthrodesis with decompressive laminectomy
Incidence of microbiological contamination of local bone autograft used in posterior lumbar interbody fusion and its association with postoperative spinal infection
Timing of Pulsed Electromagnetic Field Stimulation Does Not Affect the Promotion of Bone Cell Development
Pulsed electromagnetic field (PEMF) devices have been used clinically to promote the healing of surgically resistant fractures in vivo. However, there is a sparsity of data on how the timing of an applied pulsed electromagnetic field effects the osteogenic cells that would be present within the fracture gap. The purpose of this study was to examine the response of osteoblast-like cells to a PEMF stimulus, mimicking that of a clinically available device, using four protocols for the timing of the stimulus. The PEMF signal consisted of a 5ms pulse burst (containing 20 pulses) repeated at 15Hz. Cultures of a human osteosarcoma cell line, SaOS-2, were exposed to the four timing protocols, each conducted over three days. Protocol one stimulated the cells for eight hours each day, protocol two stimulated the cells for 24 hours on the first day, protocol three stimulated the cells for 24 hours on the second day and protocol four stimulated the cells for 24 hours on the third day. Cells were seeded with either 25,000 or 50,000 cells/well (24 well cell culture plates). All assays showed reduced proliferation and increased differentiation (alkaline phosphatase activity) in the PEMF stimulated cultures compared with the control cultures, except for protocol four alkaline phosphatase measurements. No clear trend was observed between the four protocols, however this may be due to cell density. The results indicated that an osteoblast-like cell line is responsive to a 15Hz PEMF stimulus, which will stimulate the cell line to into an increasing state of maturity
Clinical outcomes and fusion rates following anterior lumbar interbody fusion with bone graft substitute i-FACTOR, an anorganic bone matrix/P-15 composite
A Preliminary Study of the Efficacy of Beta Tricalcium Phosphate as a Bone Expander for Instrumented Posterolateral Lumbar Fusions
Pulsed Electromagnetic Field Stimulation Is a Practical Adjunctive Therapy for Fusion in Spine Surgery
Bone Marrow Seeded Bone Graft Versus Bone Graft; Compact Bone Critical Sized Defect Healing Pattern in Rabbit
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