Violação de Direitos e Medidas Protetivas na Concepção de Juízes que atuam em Juizados de Infância e Juventude

O tema da presente dissertação, desenvolvido por meio de estudo exploratório, foi a violação de direitos de crianças e adolescentes. O foco foi a análise das concepções de juízes que atuam em Juizados da Infância e Juventude, e que lidam com demandas rotineiras que lhes exigem posicionamentos diante de casos nos quais medidas de proteção se configuram necessárias. Participaram da pesquisa seis juízes de direito de Juizados da Infância e Juventude (JIJU) que atuam no Espírito Santo. Com vistas a compreender o fenômeno em tela, partiu-se da visão de que violação de direitos de crianças e de adolescentes é fenômeno multideterminado, razão pela qual foi abordado o processo histórico de constituição da família e da infância, entendidas como instituições sociais, assim como as noções de vulnerabilidade e risco social. A coleta de dados foi realizada por meio de entrevista. Dois procedimentos conduziram a sistematização e a interpretação: a Análise de Conteúdo (AC) e o software Alceste. Os resultados proporcionaram diversas constatações sobre concepções dos juízes a respeito do universo em que atuam, a saber: acreditam apresentar perfil diferenciado de outros juízes, ainda que a escolha por tal Juizado, em regra, tenha sido um acaso profissional; admitem que, em sua atuação profissional, seus valores e vivências compõem-se com seu conhecimento jurídico para nortear seu olhar acerca dos fenômenos sobre os quais precisam proferir decisão; reconhecem a importância da colaboração de outros profissionais para seu convencimento sobre os assuntos que se apresentam à sua consideração; entendem que a execução da sentença convoca diferentes instituições e profissionais a se articularem, preferencialmente no sistema de rede sócio-jurídico-assistencial; consideram que os fatores de vulnerabilidade e risco a que crianças e adolescentes estão sujeitos decorrem de fatores associados aos quais seus responsáveis também estão sujeitos, o que indica a importância da execução de políticas públicas voltadas à família; e, por fim, assinalam que além da própria família, o Estado também é violador dos direitos de crianças e adolescentes, uma vez que não planeja, de forma satisfatória, políticas públicas que propiciem mecanismos garantidores de direitos pessoais e sociais constitucionais

Dietary Cucumis melo Reduces Markers of Muscle and Articular Inflammation Following High-intensity Exercise in Horses

We evaluated the antioxidative and anti-inflammatory potential of daily oral supplementation with a proprietary powdered Cucumis melo pulp (CMP) on exercise-induced markers of articular and muscular oxidative stress and inflammation in 12 horses. Horses performed a high-intensity exercise test immediately prior to, and then following, 3 weeks of daily supplementation of 1 g powdered CMP (CMP; n=8). Controls (Co; n=8) underwent the same exercise and sampling regime but were not supplemented. Blood and synovial fluid (SF) samples were taken 24 h prior to exercise (BL), and at 1 and 24 h following exercise. Plasma and SF were analysed for prostaglandin E2 (PGE2), total antioxidant status (TAS), nitrite and superoxide dismutase (SOD) activity. SF was analysed for glycosaminoglycans (GAG), and plasma was analysed for thiobarbituric acid reactive substances (TBARS). Comparisons were made using repeated measures with the initial exercise test as a covariate. There was an increase in SF SOD activity in the CMP group. Compared to Co at 1 h, CMP reduced nitrite and GAG in SF, as well as maintained plasma TAS and lymphocyte levels. At 24 h, plasma PGE2 and creatine kinase were lower in horses receiving CMP. Three weeks of supplementation with CMP reduced markers of articular and skeletal muscle oxidative stress and inflammation in response to high-intensity exercise in horses. Nutritive antioxidants may provide a useful adjunct to the daily nutrition plan of horses undergoing regular exercise training and competition

Changes Over a Decade in Anthropometry and Fitness of Elite Austrian Youth Soccer Players

Increases in physical (e.g., high-intensity running and sprinting), technical (e.g., passing rate), and tactical (e.g., player density) aspects made elite level soccer more challenging within the past years. The aim of the study was to evaluate whether these evolutions are also been reflected in changes in anthropometric and fitness characteristics between former (2002 to 2005) and current (2012 to 2015) elite Austrian youth development center (U13 to U14) and soccer academy (U15 to U18) players. A battery of anthropometric, general and soccer-specific fitness tests was conducted annually at the end of each year. Independent t-test and Cohen’s d (ES) were calculated to compare the two four-year periods (2530 vs. 2611 players) at each age group separately. Current players were significantly faster in 20 m sprint (ES = 0.26–0.50) and reaction test (ES = 0.15–0.39, except for U18), but less flexible at sit-and-reach (ES = –0.19 to –0.55), in all age categories. Whereas height (ES = 0.26–0.32), body mass (ES = 0.11–0.18) and countermovement jump (ES = 0.24–0.26) increased significantly at youth development center level, current academy players performed superior at shuttle sprint (ES = 0.21–0.59), hurdles agility run (ES = 0.24–0.49), and endurance run (ES = 0.11–0.20). These changes over time in speed, change-of-direction ability, lower-body power, coordination, and endurance were attributed to modern training approaches (e.g., modified games and change-of-direction drills) and modifications in selection politics (e.g., coaches favor speed and decision-making skills)

Extracellular Charge Adsorption Influences Intracellular Electrochemical Homeostasis in Amphibian Skeletal Muscle

The membrane potential measured by intracellular electrodes, Em, is the sum of the transmembrane potential difference (E1) between inner and outer cell membrane surfaces and a smaller potential difference (E2) between a volume containing fixed charges on or near the outer membrane surface and the bulk extracellular space. This study investigates the influence of E2 upon transmembrane ion fluxes, and hence cellular electrochemical homeostasis, using an integrative approach that combines computational and experimental methods. First, analytic equations were developed to calculate the influence of charges constrained within a three-dimensional glycocalyceal matrix enveloping the cell membrane outer surface upon local electrical potentials and ion concentrations. Electron microscopy confirmed predictions of these equations that extracellular charge adsorption influences glycocalyceal volume. Second, the novel analytic glycocalyx formulation was incorporated into the charge-difference cellular model of Fraser and Huang to simulate the influence of extracellular fixed charges upon intracellular ionic homeostasis. Experimental measurements of Em supported the resulting predictions that an increased magnitude of extracellular fixed charge increases net transmembrane ionic leak currents, resulting in either a compensatory increase in Na+/K+-ATPase activity, or, in cells with reduced Na+/K+-ATPase activity, a partial dissipation of transmembrane ionic gradients and depolarization of Em

A mathematical model for breath gas analysis of volatile organic compounds with special emphasis on acetone

Recommended standardized procedures for determining exhaled lower respiratory nitric oxide and nasal nitric oxide have been developed by task forces of the European Respiratory Society and the American Thoracic Society. These recommendations have paved the way for the measurement of nitric oxide to become a diagnostic tool for specific clinical applications. It would be desirable to develop similar guidelines for the sampling of other trace gases in exhaled breath, especially volatile organic compounds (VOCs) which reflect ongoing metabolism. The concentrations of water-soluble, blood-borne substances in exhaled breath are influenced by: (i) breathing patterns affecting gas exchange in the conducting airways; (ii) the concentrations in the tracheo-bronchial lining fluid; (iii) the alveolar and systemic concentrations of the compound. The classical Farhi equation takes only the alveolar concentrations into account. Real-time measurements of acetone in end-tidal breath under an ergometer challenge show characteristics which cannot be explained within the Farhi setting. Here we develop a compartment model that reliably captures these profiles and is capable of relating breath to the systemic concentrations of acetone. By comparison with experimental data it is inferred that the major part of variability in breath acetone concentrations (e.g., in response to moderate exercise or altered breathing patterns) can be attributed to airway gas exchange, with minimal changes of the underlying blood and tissue concentrations. Moreover, it is deduced that measured end-tidal breath concentrations of acetone determined during resting conditions and free breathing will be rather poor indicators for endogenous levels. Particularly, the current formulation includes the classical Farhi and the Scheid series inhomogeneity model as special limiting cases.Comment: 38 page

Localized helium excitations in 4He_N-benzene clusters

We compute ground and excited state properties of small helium clusters 4He_N containing a single benzene impurity molecule. Ground-state structures and energies are obtained for N=1,2,3,14 from importance-sampled, rigid-body diffusion Monte Carlo (DMC). Excited state energies due to helium vibrational motion near the molecule surface are evaluated using the projection operator, imaginary time spectral evolution (POITSE) method. We find excitation energies of up to ~23 K above the ground state. These states all possess vibrational character of helium atoms in a highly anisotropic potential due to the aromatic molecule, and can be categorized in terms of localized and collective vibrational modes. These results appear to provide precursors for a transition from localized to collective helium excitations at molecular nanosubstrates of increasing size. We discuss the implications of these results for analysis of anomalous spectral features in recent spectroscopic studies of large aromatic molecules in helium clusters.Comment: 15 pages, 5 figures, submitted to Phys. Rev.

An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carrier, ion-selective microelectrodes

We describe an improved, efficient and reliable method for the vapour-phase silanization of multi-barreled, ion-selective microelectrodes of which the silanized barrel(s) are to be filled with neutral liquid ion-exchanger (LIX). The technique employs a metal manifold to exclusively and simultaneously deliver dimethyldichlorosilane to only the ion-selective barrels of several multi-barreled microelectrodes. Compared to previously published methods the technique requires fewer procedural steps, less handling of individual microelectrodes, improved reproducibility of silanization of the selected microelectrode barrels and employs standard borosilicate tubing rather than the less-conventional theta-type glass. The electrodes remain stable for up to 3 weeks after the silanization procedure. The efficacy of a double-barreled electrode containing a proton ionophore in the ion-selective barrel is demonstrated in situ in the leaf apoplasm of pea (Pisum) and sunflower (Helianthus). Individual leaves were penetrated to depth of ~150 μm through the abaxial surface. Microelectrode readings remained stable after multiple impalements without the need for a stabilizing PVC matrix

The abundant marine bacterium Pelagibacter simultaneously catabolizes dimethylsulfoniopropionate to the gases dimethyl sulfide and methanethiol

Marine phytoplankton produce ~109 tons of dimethylsulfoniopropionate (DMSP) per year1,2, an estimated 10% of which is catabolized by bacteria through the DMSP cleavage pathway to the climatically active gas dimethyl sulfide (DMS)3,4. SAR11 Alphaproteobacteria (order Pelagibacterales), the most abundant chemoorganotrophic bacteria in the oceans, have been shown to assimilate DMSP into biomass, thereby supplying this cell’s unusual requirement for reduced sulfur5,6. Here we report that Pelagibacter HTCC1062 produces the gas methanethiol (MeSH) and that simultaneously a second DMSP catabolic pathway, mediated by a cupin-like DMSP lyase, DddK, shunts as much as 59% of DMSP uptake to DMS production. We propose a model in which the allocation of DMSP between these pathways is kinetically controlled to release increasing amounts of DMS as the supply of DMSP exceeds cellular sulfur demands for biosynthesis