Adult Student Satisfaction with Overall Learning Experiences at East Tennessee State University.

This study examined adult students\u27 perceptions of satisifaction levels within specific areas of the ETSU campus. The population surveyed included 106 undergraduate adult learners aged 25 years and older. The Noel Levitz Student Satisfaction Inventory, 4-year College and University Version was the survey instrument used to share feedback about the adult learners\u27 collegiate experiences thus far. Responses received would give campus leadership feedback about the satisfaction levels of adult learners at ETSU. The purpose of this study was to determine if the adult learner at ETSU is satisfied with his/her overall learning experience. Adult students were asked to rank programs and services offered at ETSU and how they felt they may or may not have contributed to their overall learning experiences. Age was the determinant for the sample. Survey data from adult learners, 25 years and above, was included in this study. This study employed descriptive statistics using frequency distributions to analyze the data. Because this was a cluster sample, frequency distributions were used to report the number of times the value occurs and the percentage of respondents in each category. In order to analyze data received, questions were broken down and placed into one of seven categories used to answer the research questions. The seven categories were overall learning experience, bookstore services, food service, academic programs and services, administrative programs, caring campus, and campus commitment to student learning. Results of the data revealed that the majority of adult learners at East Tennessee State University were satisfied with all aspects surveyed on the campus and the institutions\u27s commitment to adult learner programs and services. Although all aspects surveyed on campus received high satisfaction levels, areas such as food service, counseling staff, financial aid, and creating a sense of belonging indicated lower satisfaction levels. These areas could be researched further in order to increase adult learner satisfaction levels at East Tennessee State University

The Relationship between Spirituality and the Health of College Students in a University Setting

The purpose of this study was to examine the relationship between spirituality and the health of college students. Undergraduate students enrolled in Personal Health and Wellness classes at The University of Tennessee were selected to participate in the study. Two-hundred twenty-one students were administered two instruments: The College Student Appraisal of Risks Survey (The CARS) and the Spirituality Scale (SS). The CARS instrument was used to obtain descriptive statistics and specific health risk factors relating to the health of college students. The SS instrument was used to obtain the student’s self-reported level of spirituality. Based upon a thorough literature review, there has been limited research into the relationship of the health of college students and their level of spirituality. This study does two things. First, it reports the development of a valid and reliable instrument to measure spirituality. Secondly, this study investigates the relationship between the self-reported level of spirituality and the health status of college students. The significance of the study is that this research is an important step toward understanding the role that spirituality plays in the various dimensions of health in young adults

A Study of Nontraditional Undergraduate Students at the University of Memphis.

This study examined nontraditional student engagement into the collegiate environment on the University of Memphis (U of M) campus, specifically services and programs in the University College. The sample surveyed included 4 nontraditional undergraduate students, 1 from each grade level, aged 25 years or older. The 4 nontraditional undergraduate study participants were admitted and enrolled at the U of M. Additionally, there was 1 alumnae member included in the study. The study employed the use of multiple forms of data collection including interviews, personal and focus group, journaling, life stories, and an online campus climate survey. In order to analyze the multiple forms of data received, data analyses were broken down into 3 chapters. Each chapter revealed findings that provided answers to the initial research question. Each chapter was then further divided into themes or categories that emerged from questions and interviews. Results of the research revealed that nontraditional students did not feel active engagement with the collegiate environment was a major component contributing to the success of their academic career. Research data indicated that nontraditional students because of their maturity level are at times disappointed with their traditional counterparts and faculty in classroom activities. Further, data analyzed support nontraditional student need for more communication from campus staff as well as development of separate tutoring services and offices for nontraditional students. Additionally, findings indicated that the institution should hire faculty and staff who understand nontraditional students\u27 learning styles and needs

Engineering the vaccinia virus L1 protein for increased neutralizing antibody response after DNA immunization

<p>Abstract</p> <p>Background</p> <p>The licensed smallpox vaccine, comprised of infectious vaccinia virus, has associated adverse effects, particularly for immunocompromised individuals. Therefore, safer DNA and protein vaccines are being investigated. The L1 protein, a component of the mature virion membrane that is conserved in all sequenced poxviruses, is required for vaccinia virus entry into host cells and is a target for neutralizing antibody. When expressed by vaccinia virus, the unglycosylated, myristoylated L1 protein attaches to the viral membrane via a C-terminal transmembrane anchor without traversing the secretory pathway. The purpose of the present study was to investigate modifications of the gene expressing the L1 protein that would increase immunogenicity in mice when delivered by a gene gun.</p> <p>Results</p> <p>The L1 gene was codon modified for optimal expression in mammalian cells and potential N-glycosylation sites removed. Addition of a signal sequence to the N-terminus of L1 increased cell surface expression as shown by confocal microscopy and flow cytometry of transfected cells. Removal of the transmembrane domain led to secretion of L1 into the medium. Induction of binding and neutralizing antibodies in mice was enhanced by gene gun delivery of L1 containing the signal sequence with or without the transmembrane domain. Each L1 construct partially protected mice against weight loss caused by intranasal administration of vaccinia virus.</p> <p>Conclusion</p> <p>Modifications of the vaccinia virus L1 gene including codon optimization and addition of a signal sequence with or without deletion of the transmembrane domain can enhance the neutralizing antibody response of a DNA vaccine.</p

Attitudes Toward Breast Cancer Genetic Testing in Five Special Population Groups

Purpose: This study examined interest in and attitudes toward genetic testing in 5 different population groups. Methods: The survey included African American, Asian American, Latina, Native American, and Appalachian women with varying familial histories of breast cancer. A total of 49 women were interviewed in person. Descriptive and nonparametric statistical techniques were used to assess ethnic group differences. Results: Overall, interest in testing was high. All groups endorsed more benefits than risks. There were group differences regarding endorsement of specific benefits and risks: testing to “follow doctor recommendations” (p=0.017), “concern for effects on family” (p=0.044), “distrust of modern medicine” (p=0.036), “cost” (p=0.025), and “concerns about communication of results to others” (p=0.032). There was a significant inverse relationship between interest and genetic testing cost (p Conclusion: Cost may be an important barrier to obtaining genetic testing services, and participants would benefit by genetic counseling that incorporates the unique cultural values and beliefs of each group to create an individualized, culturally competent program. Further research about attitudes toward genetic testing is needed among Asian Americans, Native Americans, and Appalachians for whom data are severely lacking. Future study of the different Latina perceptions toward genetic testing are encouraged

Replication-Deficient Vaccinia Virus Encoding Bacteriophage T7 RNA Polymerase for Transient Gene Expression in Mammalian Cells

AbstractThe vaccinia virus/bacteriophage T7 hybrid transient expression system employs a recombinant vaccinia virus that encodes the T7 RNA polymerase gene, a plasmid vector with a gene of interest regulated by a T7 promoter, and any cell line suitable for infection and transfection. Although high expression in a majority of cells is achieved. the severe cytopathic effects of vaccinia virus and the safety precautions required for use of infectious agents are undesirable features of the system. Here, we report the construction of a highly attenuated and avian host-restricted vaccinia virus recombinant that encodes the T7 RNA polymerase gene (MVA/T7 pol) and demonstrate the use of the virus for transient expression in mammalian cells. MVA/T7 pol has reduced cytopathic effects compared to the previously used replication-competent vaccinia virus, while providing a high level of gene expression in multiple mammalian cell lines

Effects of Coral Reef Benthic Primary Producers on Dissolved Organic Carbon and Microbial Activity

Benthic primary producers in marine ecosystems may significantly alter biogeochemical cycling and microbial processes in their surrounding environment. To examine these interactions, we studied dissolved organic matter release by dominant benthic taxa and subsequent microbial remineralization in the lagoonal reefs of Moorea, French Polynesia. Rates of photosynthesis, respiration, and dissolved organic carbon (DOC) release were assessed for several common benthic reef organisms from the backreef habitat. We assessed microbial community response to dissolved exudates of each benthic producer by measuring bacterioplankton growth, respiration, and DOC drawdown in two-day dark dilution culture incubations. Experiments were conducted for six benthic producers: three species of macroalgae (each representing a different algal phylum: Turbinaria ornata – Ochrophyta; Amansia rhodantha – Rhodophyta; Halimeda opuntia – Chlorophyta), a mixed assemblage of turf algae, a species of crustose coralline algae (Hydrolithon reinboldii) and a dominant hermatypic coral (Porites lobata). Our results show that all five types of algae, but not the coral, exuded significant amounts of labile DOC into their surrounding environment. In general, primary producers with the highest rates of photosynthesis released the most DOC and yielded the greatest bacterioplankton growth; turf algae produced nearly twice as much DOC per unit surface area than the other benthic producers (14.0±2.8 µmol h−1 dm−2), stimulating rapid bacterioplankton growth (0.044±0.002 log10 cells h−1) and concomitant oxygen drawdown (0.16±0.05 µmol L−1 h−1 dm−2). Our results demonstrate that benthic reef algae can release a significant fraction of their photosynthetically-fixed carbon as DOC, these release rates vary by species, and this DOC is available to and consumed by reef associated microbes. These data provide compelling evidence that benthic primary producers differentially influence reef microbial dynamics and biogeochemical parameters (i.e., DOC and oxygen availability, bacterial abundance and metabolism) in coral reef communities

Clinically relevant enhancement of human sperm motility using compounds with reported phosphodiesterase inhibitor activity

STUDY QUESTION: Can we identify compound(s) with reported phosphodiesterase inhibitor (PDEI) activity that could be added to human spermatozoa in vitro to enhance their motility without compromising other sperm functions? SUMMARY ANSWER: We have identified several compounds that produce robust and effective stimulation of sperm motility and, importantly, have a positive response on patient samples. WHAT IS KNOWN ALREADY: For &gt;20 years, the use of non-selective PDEIs, such as pentoxifylline, has been known to influence the motility of human spermatozoa; however, conflicting results have been obtained. It is now clear that human sperm express several different phosphodiesterases and these are compartmentalized at different regions of the cells. By using type-specific PDEIs, differential modulation of sperm motility may be achieved without adversely affecting other functions such as the acrosome reaction (AR). STUDY DESIGN, SIZE, DURATION: This was a basic medical research study examining sperm samples from normozoospermic donors and subfertile patients attending the Assisted Conception Unit (ACU), Ninewells Hospital Dundee for diagnostic semen analysis, IVF and ICSI. Phase 1 screened 43 commercially available compounds with reported PDEI activity to identify lead compounds that stimulate sperm motility. Samples were exposed (20 min) to three concentrations (1, 10 and 100 µM) of compound, and selected candidates (n = 6) progressed to Phase 2, which provided a more comprehensive assessment using a battery of in vitro sperm function tests.  PARTICIPANTS/MATERIALS, SETTING, METHODS: All healthy donors and subfertile patients were recruited at the Medical Research Institute, University of Dundee and ACU, Ninewells Hospital Dundee (ethical approval 08/S1402/6). In Phase 1, poor motility cells recovered from the 40% interface of the discontinuous density gradient were used as surrogates for patient samples. Pooled samples from three to four different donors were utilized in order to reduce variability and increase the number of cells available for simultaneous examination of multiple compounds. During Phase 2 testing, semen samples from 23 patients attending for either routine diagnostic andrology assessment or IVF/ICSI were prepared and exposed to selected compounds. Additionally, 48 aliquots of prepared samples, surplus to clinical use, were examined from IVF (n = 32) and ICSI (n = 16) patients to further determine the effects of selected compounds under clinical conditions of treatment. Effects of compounds on sperm motility were assessed by computer-assisted sperm analysis. A modified Kremer test using methyl cellulose was used to assess sperm functional ability to penetrate into viscous media. Sperm acrosome integrity and induction of apoptosis were assessed using the acrosomal content marker PSA-FITC and annexin V kit, respectively. MAIN RESULTS AND THE ROLE OF CHANCE: In Phase 1, six compounds were found to have a strong effect on poor motility samples with a magnitude of response of ≥60% increase in percentage total motility. Under capacitating and non-capacitating conditions, these compounds significantly (P ≤ 0.05) increased the percentage of total and progressive motility. Furthermore, these compounds enhanced penetration into a cervical mucus substitute (P ≤ 0.05). Finally, the AR was not significantly induced and these compounds did not significantly increase the externalization of phosphatidylserine (P = 0.6, respectively). In general, the six compounds maintained the stimulation of motility over long periods of time (180 min) and their effects were still observed after their removal. In examinations of clinical samples, there was a general observation of a more significant stimulation of sperm motility in samples with lower baseline motility. In ICSI samples, compounds #26, #37 and #38 were the most effective at significantly increasing total motility (88, 81 and 79% of samples, respectively) and progressive motility (94, 93 and 81% of samples, respectively). In conclusion, using a two-phased drug discovery screening approach including the examination of clinical samples, 3/43 compounds were identified as promising candidates for further study. LIMITATIONS, REASONS FOR CAUTION: This is an in vitro study and caution must be taken when extrapolating the results. Data for patients were from one assessment and thus the robustness of responses needs to be established. The n values for ICSI samples were relatively small. WIDER IMPLICATIONS OF THE FINDINGS: We have systematically screened and identified several compounds that have robust and effective stimulation (i.e. functional significance with longevity and no toxicity) of total and progressive motility under clinical conditions of treatment. These compounds could be clinical candidates with possibilities in terms of assisted reproductive technology options for current or future patients affected by asthenozoospermia or oligoasthenozoospermia

Geometrical and Physical Properties of Circumbinary Discs in Eccentric Stellar Binaries

In a previous work (Pichardo et al. 2005), we studied stable configurations for circumstellar discs in eccentric binary systems. We searched for "invariant loops": closed curves (analogous to stable periodic orbits in time-independent potentials) that change shape with the binary orbital phase, as test particles in them move under the influence of the binary potential. This approach allows us to identify stable configurations when pressure forces are unimportant, and dissipation acts only to prevent gas clouds from colliding with one another. We now extend this work to study the main geometrical properties of circumbinary discs. We have studied more than 100 cases with a range in eccentricity 0 .le. e .le. 0.9, and mass ratio 0.1 .le. q .le. 0.9. Although gas dynamics may impose further restrictions, our study sets lower stable bounds for the size of the central hole in a simple and computationally cheap way, with a relation that depends on the eccentricity and mass ratio of the central binary. We extend our previous studies and focus on an important component of these systems: circumbinary discs. The radii for stable orbits that can host gas in circumbinary discs are sharply constrained as a function of the binary's eccentricity. The circumbinary disc configurations are almost circular, with eccentricity e_d < 0.15, but if the mass ratio is unequal the disk is offset from the center of mass of the system. We compare our results with other models, and with observations of specific systems like GG Tauri A, UY Aurigae, HD 98800 B, and Fomalhaut, restricting the plausible parameters for the binary.Comment: 12 pages, 10 figures and 6 tables. MNRAS, accepte