PITTAYA LIMBUT

随着中国经济的持续快速发展和国际地位的不断提升，来华学习汉语的外国学生人数愈来愈多增长，在这种情况下，汉语学习者的学习动机已经成为国内外对外汉语教学界关注的热点课题。学习动机是影响学习者在第二语言学习外语中的关键因素之一，同时也对泰国留学生的汉语学习有着重要的影响。因此对泰国留学生汉语学习动机类型的调查研究，将能够促进对外汉语教学质量更有很大的进步，而且能够提供一些有参考价值的建议。 本研究以泰国留学生为研究对象，笔者对国内外关于第二语言学习动机的理论进行阐述，并结合泰国留学生汉语学习的实际情况，编制了泰国留学生的调查问卷，通过第一次预测发放的问卷后修改形成为正式调查问卷，对87名厦门大学的...Along with the deepening of China's sustained and rapid economic development and the improvement of the international status, the number of foreign students learning Chinese in China is continuing to grow more and more. In this case, the motivation of those learning Chinese has become a hot topic within the field of teaching Chinese as a foreign language, in both home and abroad sectors. Motivati...学位：教育硕士院系专业：海外教育学院_汉语国际教育硕士学号：2652011115430

แอฟฟินิตีไบโอเซอร์อาศัยหลักการวัดทางไฟฟ้าเคมี

Thesis (Ph.D., Analytical Chemistry)--Prince of Songkla University, 200

Disposable Electrodes for Capacitive Immunosensor

Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm x 25.4 mm) and used in a label-free immunosensor. Human Serum albumin (HSA) and its antigen (anti-HSA) were used as a model system. Electropolymerization of o-phenylenediamine was used for the immobilization of anti-HSA by covalent binding. A linear relationship was obtained in the range from 1.0 x 10-(14) to 1.0 x 10-(19) M with a limit of detection of 8.0 x 10(-15) M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method

Capacitive biosensor for detection of endotoxin

A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 x 10(-13) M and linearity ranging from 1.0 x 10(-13) to 1.0 x 10(-10) M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte lysate test as a reference. The capacitive biosensor method was statistically tested with the Wilcoxon signed rank test, which proved the system is acceptable for the quantitative analysis of bacterial endotoxin (P < 0.05)

A reusable capacitive immunosensor for carcinoembryonic antigen (CEA) detection using thiourea modified gold electrode

A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow injection capacitive immunosensor system were compared to those obtained using an enzyme linked fluorescent assay (ELFA) method (P < 0.05). (c) 2006 Elsevier B.V. All rights reserved

A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid, and 3-mercaptopropionic acid

A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled 3-mercaptopropionic acid monolayer (SAMPAM). The system is specific for alpha-fetoprotein and can be regenerated and reused up to 48 times. Therefore, self-assembled monolayer using thiourea which is cheaper than thioctic acid and 3-mercaptopropionic acid is a good alternative for biosensor applications when SAMs are used. (c) 2006 Elsevier B.V. All rights reserved

Evaluation on the Intrinsic Physicoelectrochemical Attributes and Engineering of Micro-, Nano-, and 2D-Structured Allotropic Carbon-Based Papers for Flexible Electronics

Flexible electronics have gained more attention for emerging electronic devices such as sensors, biosensors, and batteries with advantageous properties including being thin, lightweight, flexible, and low-cost. The development of various forms of allotropic carbon papers provided a new dry-manufacturing route for the fabrication of flexible and wearable electronics, while the electrochemical performance and the bending stability are largely influenced by the bulk morphology and the micro-/nanostructured domains of the carbon papers. Here, we evaluate systematically the intrinsic physicoelectrochemical properties of allotropic carbon-based conducting papers as flexible electrodes including carbon-nanotubes-paper (CNTs-paper), graphene-paper (GR-paper), and carbon-fiber-paper (CF-paper), followed by functionalization of the allotropic carbon papers for the fabrication of flexible electrodes. The morphology, chemical structure, and defects originating from the allotropic nanostructured carbon materials were characterized by scanning electron microscopy (SEM) and Raman spectroscopy, followed by evaluating the electrochemical performance of the corresponding flexible electrodes by cyclic voltammetry and electrochemical impedance spectroscopy. The electron-transfer rate constants of the CNTs-paper and GR-paper electrodes were similar to 14 times higher compared with the CF-paper electrode. The CNTs-paper and GR-paper electrodes composed of nanostructured carbon showed significantly higher bending stabilities of 5.61 and 4.96 times compared with the CF-paper. The carbon-paper flexible electrodes were further functionalized with an inorganic catalyst, Prussian blue (PB), forming the PB-carbon-paper catalytic electrode and an organic conducting polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), forming the PEDOT-carbon-paper capacitive electrode. The intrinsic attribute of different allotropic carbon electrodes affects the deposition of PB and PEDOT, leading to different electrocatalytic and capacitive performances. These findings are insightful for the future development and fabrication of advanced flexible electronics with allotropic carbon papers.Funding Agencies|Swedish Research CouncilSwedish Research CouncilEuropean Commission [VR-2015-04434]; Royal Golden Jubilee Ph.D. program (RGJ) from the Thailand Research Fund [PHD/0212/2560]</p

Adsorptive Cathodic Stripping Voltammetry for Quantification of Alprazolam

A simple and highly sensitive electrochemical sensor was developed for adsorptive cathodic stripping voltammetry of alprazolam. Based on an electrochemically pretreated glassy carbon electrode, the sensor demonstrated good adsorption and electrochemical reduction of alprazolam. The morphology of the glassy carbon electrode and the electrochemically pretreated glassy carbon electrode were characterized by scanning electron microscopy/energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The electrochemical behaviors of alprazolam were determined by cyclic voltammetry, and the analytical measurements were studied by adsorptive cathodic stripping voltammetry. Optimized operational conditions included the concentration and deposition time of sulfuric acid in the electrochemical pretreatment, preconcentration potential, and preconcentration time. Under optimal conditions, the developed alprazolam sensor displayed a quantification limit of 0.1 mg L−1, a detection limit of 0.03 mg L−1, a sensitivity of 67 µA mg−1 L cm−2 and two linear ranges: 0.1 to 4 and 4 to 20 mg L−1. Sensor selectivity was excellent, and repeatability (%RSD &lt; 4.24%) and recovery (82.0 ± 0.2 to 109.0 ± 0.3%) were good. The results of determining alprazolam in beverages with the developed system were in good agreement with results from the gas chromatography–mass spectrometric method