STUB1 suppresseses tumorigenesis and chemoresistance through antagonizing YAP1 signaling

Yes-associated protein (YAP) is a component of the canonical Hippo signaling pathway that is known to play essential roles in modulating organ size, development, and tumorigenesis. Activation or upregulation of YAP1, which contributes to cancer cell survival and chemoresistance, has been verified in different types of human cancers. However, the molecular mechanism of YAP1 upregulation in cancer is still unclear. Here we report that the E3 ubiquitin ligase STUB1 ubiquitinates and destabilizes YAP1, thereby inhibiting cancer cell survival. Low levels of STUB1 expression were correlated with increased protein levels of YAP1 in human gastric cancer cell lines and patient samples. Moreover, we revealed that STUB1 ubiquitinates YAP1 at the K280 site by K48-linked polyubiquitination, which in turn increases YAP1 turnover and promotes cellular chemosensitivity. Overall, our study establishes YAP1 ubiquitination and degradation mediated by the E3 ligase STUB1 as an important regulatory mechanism in gastric cancer, and provides a rationale for potential therapeutic interventions

The Relative Composition of the Inflammatory Infiltrate as an Additional Tool for Synovial Tissue Classification

10.1371/journal.pone.0072494PLoS ONE88-POLN

SVCEval-RA: an evaluation framework for adaptive scalable video streaming

[EN] Multimedia content adaption strategies are becoming increasingly important for effective video streaming over the actual heterogeneous networks. Thus, evaluation frameworks for adaptive video play an important role in the designing and deploying process of adaptive multimedia streaming systems. This paper describes a novel simulation framework for rate-adaptive video transmission using the Scalable Video Coding standard (H.264/SVC). Our approach uses feedback information about the available bandwidth to allow the video source to select the most suitable combination of SVC layers for the transmission of a video sequence. The proposed solution has been integrated into the network simulator NS-2 in order to support realistic network simulations. To demonstrate the usefulness of the proposed solution we perform a simulation study where a video sequence was transmitted over a three network scenarios. The experimental results show that the Adaptive SVC scheme implemented in our framework provides an efficient alternative that helps to avoid an increase in the network congestion in resource-constrained networks. Improvements in video quality, in terms of PSNR (Peak Signal to Noise Ratio) and SSIM (Structural Similarity Index) are also obtained.Castellanos Hernández, WE.; Guerri Cebollada, JC.; Arce Vila, P. (2017). SVCEval-RA: an evaluation framework for adaptive scalable video streaming. Multimedia Tools and Applications. 76(1):437-461. doi:10.1007/s11042-015-3046-yS437461761Akhshabi S, Begen AC, Dovrolis C (2011) An experimental evaluation of rate-adaptation algorithms in adaptive streaming over HTTP. In: Proceedings of the second annual ACM conference on Multimedia systems. ACM, pp 157–168Alabdulkarim MN, Rikli N-E (2012) QoS Provisioning for H.264/SVC Streams over Ad-Hoc ZigBee Networks Using Cross-Layer Design. In: 8th International Conference on Wireless Communications, Networking and Mobile Computing (WiCOM). pp 1–8Birkos K, Tselios C, Dagiuklas T, Kotsopoulos S (2013) Peer selection and scheduling of H. 264 SVC video over wireless networks. In: Wireless Communications and Networking Conference (WCNC), 2013 IEEE. pp 1633–1638Castellanos W (2014) SVCEval-RA - An Evaluation Framework for Adaptive Scalable Video Streaming. In: SourceForge Project. http://sourceforge.net/projects/svceval-ra/ . Accessed 1 May 2015Castellanos W, Guerri JC, Arce P (2015) A QoS-aware routing protocol with adaptive feedback scheme for video streaming for mobile networks. Comput Commun. http://dx.doi.org/10.1016/j.comcom.2015.08.012Castellanos W, Arce P, Acelas P, Guerri JC (2012) Route Recovery Algorithm for QoS-Aware Routing in MANETs. Springer Berlin Heidelberg, Bilbao, pp. 81–93Chikkerur S, Sundaram V, Reisslein M, Karam LJ (2011) Objective video quality assessment methods: A classification, review, and performance comparison. Broadcast, IEEE Trans on 57:165–182Choupani R, Wong S, Tolun M (2014) Multiple description coding for SNR scalable video transmission over unreliable networks. Multimed Tools Appl 69:843–858. doi: 10.1007/s11042-012-1150-9CISCO Corp. (2014) Cisco Visual Networking Index Forecast and Methodology. In: White Paper. http://www.cisco.com/c/en/us/solutions/collateral/service-provider/ip-ngn-ip-next-generation-network/white_paper_c11-481360.pdf.Dai M, Zhang Y, Loguinov D (2009) A unified traffic model for MPEG-4 and H. 264 video traces. IEEE Trans Multimedia 11:1010–1023Detti A, Bianchi G, Pisa C, et al. (2009) SVEF: an open-source experimental evaluation framework for H.264 scalable video streaming. In: IEEE Symposium on Computers and Communications. pp 36–41Espina F, Morato D, Izal M, Magaña E (2014) Analytical model for MPEG video frame loss rates and playback interruptions on packet networks. Multimed Tools Appl 72:361–383. doi: 10.1007/s11042-012-1344-1Fiems D, Steyaert B, Bruneel H (2012) A genetic approach to Markovian characterisation of H.264 scalable video. Multimedia Tools Appl 58:125–146Floyd S, Handley M, Kohler E Datagram Congestion Control Protocol (DCCP). http://tools.ietf.org/html/rfc4340 . Accessed 17 Feb 2014Floyd S, Padhye J, Widmer J TCP Friendly Rate Control (TFRC): Protocol Specification. http://tools.ietf.org/html/rfc5348 . Accessed 17 Feb 2014Fraz M, Malkani YA, Elahi MA (2009) Design and implementation of real time video streaming and ROI transmission system using RTP on an embedded digital signal processing (DSP) platform. In: 2nd International Conference on Computer, Control and Communication, 2009. IC4 2009. pp 1–6ISO/IEC (2014) Information technology - Dynamic adaptive streaming over HTTP (DASH) - Part 1: Media presentation description and segment formats.ITU-T (2013) Rec. H.264 & ISO/IEC 14496-10 AVC. Advanced Video Coding for Generic Audiovisual Services.Ivrlač MT, Choi LU, Steinbach E, Nossek JA (2009) Models and analysis of streaming video transmission over wireless fading channels. Signal Process Image Commun 24:651–665. doi: 10.1016/j.image.2009.04.005Karki R, Seenivasan T, Claypool M, Kinicki R (2010) Performance Analysis of Home Streaming Video Using Orb. In: Proceedings of the 20th International Workshop on Network and Operating Systems Support for Digital Audio and Video. ACM, New York, NY, USA, pp 111–116Ke C-H (2012) myEvalSVC-an Integrated Simulation Framework for Evaluation of H. 264/SVC Transmission. KSII Trans Internet Inf Syst (TIIS) 6:377–392. doi: 10.3837/tiis.2012.01.021Ke C-H, Shieh C-K, Hwang W-S, Ziviani A (2008) An Evaluation Framework for More Realistic Simulations of MPEG Video Transmission. J Inf Sci Eng 24:425–440Klaue J, Rathke B, Wolisz A (2003) Evalvid–A framework for video transmission and quality evaluation. In: Computer Performance Evaluation. Modelling Techniques and Tools. Springer, pp 255–272Le TA, Nguyen H (2014) End-to-end transmission of scalable video contents: performance evaluation over EvalSVC—a new open-source evaluation platform. Multimed Tools Appl 72:1239–1256. doi: 10.1007/s11042-013-1444-6Lie A, Klaue J (2008) Evalvid-RA: trace driven simulation of rate adaptive MPEG-4 VBR video. Multimedia Systems 14:33–50. doi: 10.1007/s00530-007-0110-0Moving Pictures Experts Group and ITU-T Video Coding Experts Group (2011) H. 264/SVC reference software (JSVM 9.19.14) and Manual.Nightingale J, Wang Q, Grecos C (2014) Empirical evaluation of H.264/SVC streaming in resource-constrained multihomed mobile networks. Multimed Tools Appl 70:2011–2035. doi: 10.1007/s11042-012-1219-5Parmar H, Thornburgh M (2012) Real-Time Messaging Protocol (RTMP) Specification. AdobePolitis I, Dounis L, Dagiuklas T (2012) H. 264/SVC vs. H. 264/AVC video quality comparison under QoE-driven seamless handoff. Signal Process Image Commun 27:814–826Pozueco L, Pañeda XG, García R, et al. (2013) Adaptable system based on Scalable Video Coding for high-quality video service. Comput Electr Eng 39:775–789. doi: 10.1016/j.compeleceng.2013.01.015Pozueco L, Pañeda XG, García R, et al. (2014) Adaptation engine for a streaming service based on MPEG-DASH. Multimed Tools Appl 1–20. doi: 10.1007/s11042-014-2034-ySchwarz H, Marpe D, Wiegand T (2007) Overview of the Scalable Video Coding Extension of the H.264/AVC Standard. IEEE Trans Circ Syst Video Technol 17:1103–1120. doi: 10.1109/TCSVT.2007.905532Seo H-Y (2013) An Efficient Transmission Scheme of MPEG2-TS over RTP for a Hybrid DMB System. ETRI J 35:655–665. doi: 10.4218/etrij.13.0112.0124Sohn H, Yoo H, De Neve W, et al. (2010) Full-Reference Video Quality Metric for Fully Scalable and Mobile SVC Content. IEEE Trans Broadcast 56:269–280. doi: 10.1109/TBC.2010.2050628Sousa-Vieira M-E (2011) Suitability of the M/G/∞ process for modeling scalable H.264 video traffic. In: Analytical and Stochastic Modeling Techniques and Applications. Springer, pp 149–158Tanwir S, Perros H (2013) A Survey of VBR Video Traffic Models. IEEE Commun Surv Tutor 15:1778–1802. doi: 10.1109/SURV.2013.010413.00071Tanwir S, Perros HG (2014) VBR Video Traffic Models. Wiley, HobokenThe Network Simulator (NS-2). http://www.isi.edu/nsnam/ns . Accessed 6 Feb 2015Unanue I, Urteaga I, Husemann R, et al. (2011) A Tutorial on H. 264/SVC Scalable Video Coding and its Tradeoff between Quality, Coding Efficiency and Performance. Recent Advances on Video Coding 1–24.Van der Auwera G, David PT, Reisslein M, Karam LJ (2008) Traffic and quality characterization of the H. 264/AVC scalable video coding extension. Adv Multimedia 2008:1Wang Y, Claypool M (2005) RealTracer—Tools for Measuring the Performance of RealVideo on the Internet. Multimed Tools Appl 27:411–430. doi: 10.1007/s11042-005-3757-6Wang Z, Lu L, Bovik AC (2004) Video quality assessment based on structural distortion measurement. Signal Process Image Commun 19:121–132. doi: 10.1016/S0923-5965(03)00076–6Wien M, Schwarz H, Oelbaum T (2007) Performance Analysis of SVC. 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Jet energy measurement with the ATLAS detector in proton-proton collisions at root s=7 TeV

The jet energy scale and its systematic uncertainty are determined for jets measured with the ATLAS detector at the LHC in proton-proton collision data at a centre-of-mass energy of √s = 7TeV corresponding to an integrated luminosity of 38 pb-1. Jets are reconstructed with the anti-kt algorithm with distance parameters R=0. 4 or R=0. 6. Jet energy and angle corrections are determined from Monte Carlo simulations to calibrate jets with transverse momenta pT≥20 GeV and pseudorapidities {pipe}η{pipe}<4. 5. The jet energy systematic uncertainty is estimated using the single isolated hadron response measured in situ and in test-beams, exploiting the transverse momentum balance between central and forward jets in events with dijet topologies and studying systematic variations in Monte Carlo simulations. The jet energy uncertainty is less than 2. 5 % in the central calorimeter region ({pipe}η{pipe}<0. 8) for jets with 60≤pT<800 GeV, and is maximally 14 % for pT<30 GeV in the most forward region 3. 2≤{pipe}η{pipe}<4. 5. The jet energy is validated for jet transverse momenta up to 1 TeV to the level of a few percent using several in situ techniques by comparing a well-known reference such as the recoiling photon pT, the sum of the transverse momenta of tracks associated to the jet, or a system of low-pT jets recoiling against a high-pT jet. More sophisticated jet calibration schemes are presented based on calorimeter cell energy density weighting or hadronic properties of jets, aiming for an improved jet energy resolution and a reduced flavour dependence of the jet response. The systematic uncertainty of the jet energy determined from a combination of in situ techniques is consistent with the one derived from single hadron response measurements over a wide kinematic range. The nominal corrections and uncertainties are derived for isolated jets in an inclusive sample of high-pT jets. Special cases such as event topologies with close-by jets, or selections of samples with an enhanced content of jets originating from light quarks, heavy quarks or gluons are also discussed and the corresponding uncertainties are determined. © 2013 CERN for the benefit of the ATLAS collaboration

Measurement of the inclusive and dijet cross-sections of b-jets in pp collisions at sqrt(s) = 7 TeV with the ATLAS detector

The inclusive and dijet production cross-sections have been measured for jets containing b-hadrons (b-jets) in proton-proton collisions at a centre-of-mass energy of sqrt(s) = 7 TeV, using the ATLAS detector at the LHC. The measurements use data corresponding to an integrated luminosity of 34 pb^-1. The b-jets are identified using either a lifetime-based method, where secondary decay vertices of b-hadrons in jets are reconstructed using information from the tracking detectors, or a muon-based method where the presence of a muon is used to identify semileptonic decays of b-hadrons inside jets. The inclusive b-jet cross-section is measured as a function of transverse momentum in the range 20 < pT < 400 GeV and rapidity in the range |y| < 2.1. The bbbar-dijet cross-section is measured as a function of the dijet invariant mass in the range 110 < m_jj < 760 GeV, the azimuthal angle difference between the two jets and the angular variable chi in two dijet mass regions. The results are compared with next-to-leading-order QCD predictions. Good agreement is observed between the measured cross-sections and the predictions obtained using POWHEG + Pythia. MC@NLO + Herwig shows good agreement with the measured bbbar-dijet cross-section. However, it does not reproduce the measured inclusive cross-section well, particularly for central b-jets with large transverse momenta.Comment: 10 pages plus author list (21 pages total), 8 figures, 1 table, final version published in European Physical Journal