10.1371/journal.pone.0072494PLoS ONE88-POLN

A Ogdie

C Diaz-Torne

Claudia Pommerenke

Cristina Della Beffa

E Slansky

Elisabeth Slansky

F Pessler

Frank Klawonn

Frank Pessler

H. Ralph Schumacher

J Li

Jialiang Li

JJ Haringman

L Morawietz

Lie Dai

M Hall

MC Kraan

Pierre Bobé

PO van der Hall

S Oehler

T Rooney

V Krenn

ZN Jahangier

PLoS ONE

PubMed

Crossref

The Relative Composition of the Inflammatory Infiltrate as an Additional Tool for Synovial Tissue Classification

<div>ObjectivesTraditionally, differences in absolute numbers of cells expressing a certain marker (e.g., positive staining cells per mm2) have been used in immunohistological synovial tissue classification. We have begun to evaluate the relative composition of the inflammatory infiltrates, i.e. percentages of inflammatory cell types in inflammatory infiltrates, as an alternate classification tool that may potentially improve tissue diagnostics, subgrouping in clinical trials, and understanding of pathogenesis of inflammatory and noninflammatory arthropathies. MethodsSynovial tissue specimens (normal synovium, n=15; orthopedic arthropathies, n=6; osteoarthritis, n=26; early undifferentiated arthritis, n=10; rheumatoid arthritis, n=26; chronic septic arthritis, n=11) were stained for CD15, CD68, CD3, CD20, and CD38. Densities of cells expressing a given marker were determined in the superficial subintima. Binary and multicategory receiver operating characteristic (ROC) analysis and naïve Bayes classifier were used to compare the abilities of (1) the absolute densities of cells expressing a given marker (absolute method) with (2) the percentages of these cells in the inflammatory cell population (relative method) to differentiate among the six tissue classes. ResultsThe inflammatory infiltrates in normal synovium and the orthopedic arthropathies consisted almost exclusively of CD68+ and CD3+ cells. Notable fractions of CD20+ and CD38+ cells appeared in a subset of osteoarthritis samples, and increased further in early, rheumatoid and chronic septic arthritis. ROC analyses and naïve Bayes classifier ranked the absolute method above the relative method in terms of overall discriminatory ability. The relative method became slightly superior when the samples were also stratified according to the total number of inflammatory cells/mm2. ConclusionsThis exploratory investigation featuring a variety of joint disorders revealed that measuring the relative proportions of inflammatory cell types may aid in synovial tissue classification if the samples are also stratified according to the intensity of inflammation. </div

Cristina Della Beffa (443557)

Elisabeth Slansky (443558)

Claudia Pommerenke (150761)

Frank Klawonn (19279)

Jialiang Li (31220)

Lie Dai (52004)

H. Ralph Schumacher Jr. (443559)

Frank Pessler (52000)

The Francis Crick Institute

) have been used in immunohistological synovial tissue classification. We have begun to evaluate the relative composition of the inflammatory infiltrates, i.e. percentages of inflammatory cell types in inflammatory infiltrates, as an alternate classification tool that may potentially improve tissue diagnostics, subgrouping in clinical trials, and understanding of pathogenesis of inflammatory and noninflammatory arthropathies.Synovial tissue specimens (normal synovium, n=15; orthopedic arthropathies, n=6; osteoarthritis, n=26; early undifferentiated arthritis, n=10; rheumatoid arthritis, n=26; chronic septic arthritis, n=11) were stained for CD15, CD68, CD3, CD20, and CD38. Densities of cells expressing a given marker were determined in the superficial subintima. Binary and multicategory receiver operating characteristic (ROC) analysis and naïve Bayes classifier were used to compare the abilities of (1) the absolute densities of cells expressing a given marker (absolute method) with (2) the percentages of these cells in the inflammatory cell population (relative method) to differentiate among the six tissue classes..This exploratory investigation featuring a variety of joint disorders revealed that measuring the relative proportions of inflammatory cell types may aid in synovial tissue classification if the samples are also stratified according to the intensity of inflammation

Della Beffa Cristina

Slansky Elisabeth

Pommerenke Claudia

Klawonn Frank

Li Jialiang

Dai Lie

Schumacher H. Ralph

Pessler Frank

Public Library of Science (PLOS)

OBJECTIVES: Traditionally, differences in absolute numbers of cells expressing a certain marker (e.g., positive staining cells per mm²) have been used in immunohistological synovial tissue classification. We have begun to evaluate the relative composition of the inflammatory infiltrates, i.e. percentages of inflammatory cell types in inflammatory infiltrates, as an alternate classification tool that may potentially improve tissue diagnostics, subgrouping in clinical trials, and understanding of pathogenesis of inflammatory and noninflammatory arthropathies. METHODS: Synovial tissue specimens (normal synovium, n=15; orthopedic arthropathies, n=6; osteoarthritis, n=26; early undifferentiated arthritis, n=10; rheumatoid arthritis, n=26; chronic septic arthritis, n=11) were stained for CD15, CD68, CD3, CD20, and CD38. Densities of cells expressing a given marker were determined in the superficial subintima. Binary and multicategory receiver operating characteristic (ROC) analysis and naïve Bayes classifier were used to compare the abilities of (1) the absolute densities of cells expressing a given marker (absolute method) with (2) the percentages of these cells in the inflammatory cell population (relative method) to differentiate among the six tissue classes. RESULTS: The inflammatory infiltrates in normal synovium and the orthopedic arthropathies consisted almost exclusively of CD68+ and CD3+ cells. Notable fractions of CD20+ and CD38+ cells appeared in a subset of osteoarthritis samples, and increased further in early, rheumatoid and chronic septic arthritis. ROC analyses and naïve Bayes classifier ranked the absolute method above the relative method in terms of overall discriminatory ability. The relative method became slightly superior when the samples were also stratified according to the total number of inflammatory cells/mm². CONCLUSIONS: This exploratory investigation featuring a variety of joint disorders revealed that measuring the relative proportions of inflammatory cell types may aid in synovial tissue classification if the samples are also stratified according to the intensity of inflammation

H Ralph Schumacher

Directory of Open Access Journals

The relative composition of the inflammatory infiltrate as an additional tool for synovial tissue classification.

Traditionally, differences in absolute numbers of cells expressing a certain marker (e.g., positive staining cells per mm(2)) have been used in immunohistological synovial tissue classification. We have begun to evaluate the relative composition of the inflammatory infiltrates, i.e. percentages of inflammatory cell types in inflammatory infiltrates, as an alternate classification tool that may potentially improve tissue diagnostics, subgrouping in clinical trials, and understanding of pathogenesis of inflammatory and noninflammatory arthropathies

Della Beffa, Cristina

Slansky, Elisabeth

Pommerenke, Claudia

Klawonn, Frank

Li, Jialiang

Dai, Lie

Schumacher, H Ralph

Pessler, Frank

Helmholtz Zentrum für Infektionsforschung Repository

http://scholarbank.nus.edu.sg/bitstream/10635/105429/1/2013-The_Relative_Composition_of_the_Inflammatory-published.PDF

The Relative Composition of the Inflammatory Infiltrate as an Additional Tool for Synovial Tissue Classification

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The Francis Crick Institute

Public Library of Science (PLOS)

Directory of Open Access Journals

Helmholtz Zentrum für Infektionsforschung Repository