Modulation of COUP-TF Expression in a Cnidarian by Ectopic Wnt Signalling and Allorecognition

COUP transcription factors are required for the regulation of gene expression underlying development, differentiation, and homeostasis. They have an evolutionarily conserved function, being a known marker for neurogenesis from cnidarians to vertebrates. A homologue of this gene was shown previously to be a neuronal and nematocyte differentiation marker in Hydra. However, COUP-TFs had not previously been studied in a colonial cnidarian.We cloned a COUP-TF homologue from the colonial marine cnidarian Hydractinia echinata. Expression of the gene was analysed during normal development, allorecognition events and ectopic Wnt activation, using in situ hybridisation and quantitative PCR. During normal Hydractinia development, the gene was first expressed in post-gastrula stages. It was undetectable in larvae, and its mRNA was present again in putative differentiating neurons and nematocytes in post-metamorphic stages. Global activation of canonical Wnt signalling in adult animals resulted in the upregulation of COUP-TF. We also monitored a strong COUP-TF upregulation in stolons undergoing allogeneic interactions. COUP-TF mRNA was most concentrated in the tissues that contacted allogeneic, non-self tissues, and decreased in a gradient away from the contact area. Interestingly, the gene was transiently upregulated during initial contact of self stolons, but dissipated rapidly following self recognition, while in non-self contacts high expression levels were maintained.We conclude that COUP-TF is likely involved in neuronal/nematocyte differentiation in a variety of contexts. This has now been shown to include allorecognition, where COUP-TF is thought to have been co-opted to mediate allorejection by recruiting stinging cells that are the effectors of cytotoxic rejection of allogeneic tissue. Our findings that Wnt activation upregulates COUP-TF expression suggests that Wnts' role in neuronal differentiation could be mediated through COUP-TF

Targeting Vascular NADPH Oxidase 1 Blocks Tumor Angiogenesis through a PPARα Mediated Mechanism

Reactive oxygen species, ROS, are regulators of endothelial cell migration, proliferation and survival, events critically involved in angiogenesis. Different isoforms of ROS-generating NOX enzymes are expressed in the vasculature and provide distinct signaling cues through differential localization and activation. We show that mice deficient in NOX1, but not NOX2 or NOX4, have impaired angiogenesis. NOX1 expression and activity is increased in primary mouse and human endothelial cells upon angiogenic stimulation. NOX1 silencing decreases endothelial cell migration and tube-like structure formation, through the inhibition of PPARα, a regulator of NF-κB. Administration of a novel NOX-specific inhibitor reduced angiogenesis and tumor growth in vivo in a PPARα dependent manner. In conclusion, vascular NOX1 is a critical mediator of angiogenesis and an attractive target for anti-angiogenic therapies

Identification of the traditional and non-traditional sulfate-reducing bacteria associated with corroded ship hull

Pitting corrosion due to microbial activity is the most severe type of corrosion that occurs in ship hull. Since biogenic sulfide produced by sulfate-reducing bacteria (SRB) is involved in the acceleration of pitting corrosion of marine vessels, so it is important to collect information about SRB community involved in maritime vessel failure. We investigated the SRB community on corroded hull portion of the ship. With the use of common cultural method and 16S rDNA sequencing, ten bacteria with sulfate reduction ability were isolated and identified. They belonged to both traditional (Desulfovibrio, Desulfotomaculum) and non-traditional (Citrobacter) sulfate-reducing bacteria. All the isolates were able to produce a high amount of sulfide. However, only traditional isolates were showing the amplification for the SRB-specific gene, dsrAB. Further studies on corrosion potential of these two groups of bacteria showed that in spite of high sulfide and biofilm production by non-traditional SRB, they are less aggressive towards the mild steel compare to the traditional group

Bridging the gap from test rooms to field-tests for human indoor comfort studies: A critical review of the sustainability potential of living laboratories

Occupants play a key role in determining final building energy consumption. Empirical evidence must support occupants’ modelling. Experiments on human responses to Indoor Environmental Quality (IEQ) are usually performed in test rooms or as in-field monitoring. Between these two approaches, living laboratories, often abbreviated as living labs, represent a valid alternative due to their resemblance to real-world settings. This allows observing actual behaviours while keeping the capability to reliably monitor and control the indoor environment. This work systematically reviewed the available information from 34 living labs for human comfort studies worldwide to define the scope, characteristics, and significance of living labs, for the first time. Most of the reviewed living labs are office environments, and only a few do not involve a university research institution in their operation and management. Most of them are in Europe and the United States, whereas there is a lack of such facilities in other locations and climate zones (e.g., tropics). A larger number of comfort studies in living labs is required to clarify the differences in the knowledge acquired in these experiments compared to in-field and laboratory ones. The review shows that living labs add opportunities for testing and optimizing innovations in human-centric solutions for comfortable green buildings. Through the living labs approach it is possible to holistically capture the influence of IEQ on occupant perception and the related response, to gather data on larger and more diverse groups of people, and to conduct multi-domain comfort studies involving multidisciplinary approaches given their real-life settings

Test rooms to study human comfort in buildings: A review of controlled experiments and facilities

Occupants' comfort perception affects building energy consumptions. To improve the understanding of human comfort, which is crucial to reduce energy demand, laboratory experiments with humans in controlled environments (test rooms) are fundamental, but their potential also depends on the characteristic of each research facility. Nowadays, there is no common understanding for definitions, concepts, and procedures related to human comfort studies performed in test rooms. Identifying common features would allow standardising test procedures, reproducing the same experiments in different contexts, and sharing knowledge and test possibilities. This review identifies 187 existing test rooms worldwide: 396 papers were systematically selected, thoroughly reviewed, and analysed in terms of performed experiments and related test room details. The review highlights a rising interest in the topic during the last years, since 46% of related papers has been published between 2016 and 2020. A growing interest in non-thermal sensory domains (such as visual and air quality) and multi-domain studies about occupant's whole comfort emerged from the results. These research trends have entailed a change in the way test rooms are designed, equipped and controlled, progressively becoming more realistic inhabitable environments. Nevertheless, some lacks in comfort investigation are highlighted: some continents (like Africa and South America) and climate zones are found to be underrepresented, while involved subjects are mainly students performing office tasks. This review aspires to guide scientists and professionals toward the improved design or the audit of test room experimental facilities, especially in countries and climate zones where human comfort indoors is under-studied.HOBE

Test rooms to study human comfort in buildings: a review of controlled experiments and facilities

Occupants’ comfort perception affects building energy consumptions. To improve the understanding of human comfort, which is crucial to reduce energy demand, laboratory experiments with humans in controlled environments (test rooms) are fundamental, but their potential also depends on the characteristic of each research facility. Nowadays, there is no common understanding for definitions, concepts, and procedures related to human comfort studies performed in test rooms. Identifying common features would allow standardising test procedures, reproducing the same experiments in different contexts, and sharing knowledge and test possibilities. This review identifies 187 existing test rooms worldwide: 396 papers were systematically selected, thoroughly reviewed, and analysed in terms of performed experiments and related test room details. The review highlights a rising interest in the topic during the last years, since 46% of related papers has been published between 2016 and 2020. A growing interest in non-thermal sensory domains (such as visual and air quality) and multi-domain studies about occupant's whole comfort emerged from the results. These research trends have entailed a change in the way test rooms are designed, equipped and controlled, progressively becoming more realistic inhabitable environments. Nevertheless, some lacks in comfort investigation are highlighted: some continents (like Africa and South America) and climate zones are found to be underrepresented, while involved subjects are mainly students performing office tasks. This review aspires to guide scientists and professionals toward the improved design or the audit of test room experimental facilities, especially in countries and climate zones where human comfort indoors is under-studied

Test rooms to study human comfort in buildings: A review of controlled experiments and facilities

Occupants’ comfort perception affects building energy consumptions. To improve the understanding of human comfort, which is crucial to reduce energy demand, laboratory experiments with humans in controlled environments (test rooms) are fundamental, but their potential also depends on the characteristic of each research facility. Nowadays, there is no common understanding for definitions, concepts, and procedures related to human comfort studies performed in test rooms. Identifying common features would allow standardising test procedures, reproducing the same experiments in different contexts, and sharing knowledge and test possibilities. This review identifies 187 existing test rooms worldwide: 396 papers were systematically selected, thoroughly reviewed, and analysed in terms of performed experiments and related test room details. The review highlights a rising interest in the topic during the last years, since 46% of related papers has been published between 2016 and 2020. A growing interest in non-thermal sensory domains (such as visual and air quality) and multi-domain studies about occupant's whole comfort emerged from the results. These research trends have entailed a change in the way test rooms are designed, equipped and controlled, progressively becoming more realistic inhabitable environments. Nevertheless, some lacks in comfort investigation are highlighted: some continents (like Africa and South America) and climate zones are found to be underrepresented, while involved subjects are mainly students performing office tasks. This review aspires to guide scientists and professionals toward the improved design or the audit of test room experimental facilities, especially in countries and climate zones where human comfort indoors is under-studied