Mapping the <i>Shh</i> long-range regulatory domain

Coordinated gene expression controlled by long-distance enhancers is orchestrated by DNA regulatory sequences involving transcription factors and layers of control mechanisms. The Shh gene and well-established regulators are an example of genomic composition in which enhancers reside in a large desert extending into neighbouring genes to control the spatiotemporal pattern of expression. Exploiting the local hopping activity of the Sleeping Beauty transposon, the lacZ reporter gene was dispersed throughout the Shh region to systematically map the genomic features responsible for expression activity. We found that enhancer activities are retained inside a genomic region that corresponds to the topological associated domain (TAD) defined by Hi-C. This domain of approximately 900 kb is in an open conformation over its length and is generally susceptible to all Shh enhancers. Similar to the distal enhancers, an enhancer residing within the Shh second intron activates the reporter gene located at distances of hundreds of kilobases away, suggesting that both proximal and distal enhancers have the capacity to survey the Shh topological domain to recognise potential promoters. The widely expressed Rnf32 gene lying within the Shh domain evades enhancer activities by a process that may be common among other housekeeping genes that reside in large regulatory domains. Finally, the boundaries of the Shh TAD do not represent the absolute expression limits of enhancer activity, as expression activity is lost stepwise at a number of genomic positions at the verges of these domains

Management Challenges for DevOps Adoption within UK SMEs

The DevOps phenomenon is gathering pace as more UK organisations seek to leverage the benefits it can potentially bring to software engineering functions. However substantial organisational change is inherent to adopting DevOps, especially where there are prior and established methods. As part of a wider piece of doctoral research investigating the management challenges of DevOps adoption, we present early findings of a six month qualitative diary study following the adoption of DevOps within a UK based SME with over 200 employees. We find that within our case study organisation, the DevOps approach is being adopted for the development of a new system used both internally and by customers. DevOps, conceptually, appears to be generally well regarded, but in reality is proving difficult to fully adopt. This difficulty is down to a combination of necessity in maintaining a legacy system, lack of senior management buy-in, managerial structure and resistance. Additionally, we are finding evidence of job crafting, especially with the software developers. Taken together, we put forward the argument that DevOps is an interdisciplinary topic which would greatly benefit from further management and potentially psychology oriented research attention

Disruptive Innovation: Enabling Practitioners to Tackle the "Innovators Dilemma" With Graphical Techniques - A Focus on Resource Allocation

This paper presents the findings of part of a 30 month investigation, conducted to better understand the persistent failure of management practitioners to fund potentially disruptive innovations. A Mode 2 case study strategy was employed. The iterative transfer of knowledge, between four industrial cases and academia, has successfully culminated in new academic understanding of disruptive innovation and guidance for practitioners. It was found that funding decisions are mainly constrained by mental not physical processes. Organisations wishing to pursue disruptive innovations can challenge psychological attachments to incrementalism, and overcome the funding barrier, with a holistic understanding delivered through graphical portfolio tools

Training Caregivers to Conduct a Descriptive Behavioral Assessment

We evaluated the efficacy of a training package in teaching caregivers to conduct an A-B-C checklist recording functional behavioral assessment. The training package consisted of a Microsoft PowerPoint presentation containing pertinent background information, a task analysis of assessment procedures, and examples of common environmental patterns that occasion and maintain challenging behavior and practice with supportive and corrective feedback. Participants conducted assessments while observing their own child engage in challenging behavior in the natural environment and while watching video recordings of other individuals engage in a variety of challenging behaviors. Data were collected on participants’ accurate marking of antecedent, behavior, and consequence events that occurred during observations. Results showed that, following training, both participants correctly identified greater than 90% of events that occurred in training videos and 100% of events that occurred in the natural environment. Implications for future research are discussed

Creative television production by community groups

Thesis (M.S.)--Boston Universit

Knowledge transfer between Food Research Institute and industry in the UK: the role of open innovation and social capital

This paper is part of a wider research project that seeks a conceptualised explanation to how and why knowledge is transferred between food research institutes and industry in the UK, by identifying the mechanisms that drive this process. It presents the findings from 13 in-depth interviews which formed the pilot study conducted through a qualitative approach involving a leading food research institute and main food retailers. An analysis of the Open Innovation and Social Capital literatures lead to the conclusion that these are plausible angles to start the study. Participation in formal and informal networks and the importance of social structures play a significant role in this context as well as different open and collaborative activities. The emergent findings suggests that innovation challenges facing this sector are around sustainability issues such as waste, climate change and food safety. Knowledge transfer between food research institute and industry occurs through open innovation activities, the main motivations being internal learning and access to funding through engagement with industry. Similarly there are many opportunities for intermediaries in the commercialisation of ideas. Moreover, the individual level of social capital provides the network structure and expertise access to engage in collaborative projects

Evaluation of rhizobacterial colonisation and the ability to induce Globodera pallida hatch

Three bacterial isolates, SB13 (Acinetobacter sp.), SB14 (Arthrobacter sp.) and SB15 (Bacillus sp.), were previously isolated from the rhizosphere of sugar beet (Beta vulgaris ssp. vulgaris) plants and shown to increase hatch of potato cyst nematodes in vitro. In this study, the three isolates were assayed for rhizosphere competence. Each isolate was applied to seeds at each of four concentrations (105-108 CFU ml−1) and the inoculated seeds were planted in plastic microcosms containing coarse sand. All three isolates were shown to colonise the rhizosphere, although to differing degrees, with the higher inoculation densities providing significantly better colonisation. The isolates increased sugar beet root and shoot dry weight. Isolates SB14 and SB15 were analysed for their ability to induce in vivo hatch of Globodera pallida in non-sterile soil planted with sugar beet. After 4 and 6 weeks, both isolates had induced significantly greater percentage hatch compared to controls

Social media interaction, the university brand and recruitment performance

yesCommentators and academics now refer to Higher Education (HE) as a market and the language of the market frames and describes the sector. Considerable competition for students exists and the marketplace is global as institutions compete for students not just from their own country, but from the lucrative international market. Universities are aware of the importance of their reputations, but to what extent are they utilizing branding activity to deal with such competitive threats? Can institutions with lower reputational capital compete for students by increasing their brand presence? This study provides evidence from research into social media related branding activity from 56 UK universities and considers the impact of this activity, in particular social media interaction and social media validation, on performance in terms of student recruitment. The results demonstrate a positive effect for the use of social media on brand performance, especially when an institution attracts a large number of Likes on Facebook and a high number of Followers on Twitter. A particularly strong and positive effect results when universities use social media interactively

Book review : Not again little owl by Dr Vivien Norris

Dr Vivien Norris, the author, is Clinical Director of the Family Place and her experience as a clinical psychologist, Theraplay and DDP practitioner is evident throughout the book. The book is the story of Little Owl whose Mummy isn't able to look after him properly. It is decided by Rabbit that he should live with his Granny but she can't keep up with his bounciness. So Little Owl is moved again by Rabbit, first to Fox and then on to Hedgehog and then eventually to Badger. Badger is able to help him and can accept him with all his speed and bounciness, and help him with his 'muddles'. Little Owl begins to relax with Badger, they fit together and he starts to feel safe. When Rabbit arrives once more to move him again Little Owl is so upset and frightened. He doesn't know Squirrel and doesn't want to move again. Badger and Rabbit accept his feelings and stay right alongside him during the move. Badger is able to tell Squirrel about all that Little Owl has been through and how hard it has been, and the special ways he needs to be looked after to make him feel safe