The behavior of some soybean varieties in the pedoclimatic conditions from the Center of Moldavia

Soybeans is an important source of protein because the amount of protein in a hectare of soybeans is higher than in any other crop. It has a wide range of uses, and it can be used in many areas: for food production, for food and as fuel. Considering the growing interest, in recent years, for this crop, at the ARDS Secuieni a number of ten soybean varieties have been experimented, Carla TD, Larisa, Caro TD, Ilinca TD, Bia TD, Ada TD, Teo TD, Miruna TD, Felicia TD, Onix, creations of the Agricultural Research and Development Station Turda, in order to establish their adaptability to the pedoclimatic conditions of the area. On average, the factors studied greatly influenced soybean production, which varied widely, from 2687 kg/ha, to Felicia TD variety to 3409 kg/ha to Larisa variety. Regarding the correlation between soybean production and the protein production obtained, it is observed that there is a direct correlation, the correlation coefficients calculated being statistically assured and interpreted as very significant

Obtained results regarding the optimization of grain sorghum technology in pedoclimatic conditions from Central of Moldova

In recent decades in Romania and in many other regions of the world, there is a high frequency of drought years, to three years out of every five. This climate negatively affects the productivity of agricultural crops, and agricultural research must find solutions to diversify the assortment of crops that in tougher environmental conditions guarantee the stability and profitability of agriculture. One of the safest solutions is the cultivation of sorghum, whether for grain or biomass, which due to good drought resistance, the ability to withstand high temperatures and capitalize on poorly fertile land is superior by the yields of all crops and especially, cereals and fodder plants. In the pedoclimatic conditions from the Center of Moldova, starting with 2018 year, were initiated some researches regarding the optimization of the cultivation technology for grain sorghum, in order to create a technology in relation to the new climatic conditions and in relation to the protection of the environment. The results obtained on average over two agricultural years, 2018 and 2019, indicated a very large variation of grain production depending on the sowing density provided and on the applied mineral fertilizers, this being between 4074 kg/ha and 10234 kg/ha. The best results were obtained in the variants sown with 30 g.g./sqm and fertilized with N150P80 with and without the application of the biostimulator Aminosol. From the point of view of economic efficiency, the variant sown with 30 g.g./sqm and fertilized with N150P80 was the most profitable

Lung cancer biomarker testing : perspective from Europe

A questionnaire on biomarker testing previously used in central European countries was extended and distributed in Western and Central European countries to the pathologists participating at the Pulmonary Pathology Society meeting 26-28 June 2019 in Dubrovnik, Croatia. Each country was represented by one responder. For recent biomarkers the availability and reimbursement of diagnoses of molecular alterations in non-small cell lung carcinoma varies widely between different, also western European, countries. Reimbursement of such assessments varies widely between unavailability and payments by the health care system or even pharmaceutical companies. The support for testing from alternative sources, such as the pharmaceutical industry, is no doubt partly compensating for the lack of public health system support, but it is not a viable or long-term solution. Ideally, a structured access to testing and reimbursement should be the aim in order to provide patients with appropriate therapeutic options. As biomarker enabled therapies deliver a 50% better probability of outcome success, improved and unbiased reimbursement remains a major challenge for the future.Peer reviewe

Research on the influence of nutrition on the production of space and quality herba Hyssopus officinalis l. (hyssop) cultivated ecological conditions in A.R.D.S. Secuieni

Hyssopus officinalis L. (hyssop) has been known as a medicinal plant since ancient times. Grown for herba contains volatile oil (0.5 and 1.3 % in dry herba between 0.10 and 0.15 % in fresh herba ), flavonoids, ursolic and oleanolic acids, carbohydrates, hisopina, tannins, resins, gums, sugars and a substance with antiviral marubiina, which gives a bitter taste. Due to the volatile oil constituents, the plant has antiseptic expectorant action and is bitter tonic (because marubiinei) and slightly astringent (due to tannins). Climatic conditions during the experiment were good, the average temperature in the two years was 9,150 C, the difference from the annual average (8.7ºC) being 0.45ºC. The amount of rainfall was 488.5 mm by 59.4 mm less than the annual average (552.1mm). The average for the two years of experimentation average number of branches per plant ranged from 14.27 branches/plant variant sown at 50 cm between rows and incineration (Mt.) and 23 branches/plant variant sown at 70 cm between rows and incineration. The average length of inflorescence was higher (12.25 cm) in variant sown distance of 70 cm between rows and 20 cm between plants in the row. Regarding the production of dry herba it was influenced by both the distance between rows (average factor A) and the distance between plants in the row (average factor B). The average for the two years of experimentation, production highest (2510 kg/ha) was obtained in the variant sown at a distance of 70 cm between rows and 20 cm between plants in the row. An average production close of 2368 kg/ha was obtained in variant sown at a distance of 70 cm between rows and 30 cm between plants in the row. Highest production of essential oil (80.88 l/ha) was obtained in the variant sown at a distance of 70 cm between rows and 20 cm between plants in the row

Researches regarding the influence of some technological elements on the yield levels of soybean crop in the Central Moldavia pedoclimatic conditions paper title

The laborious researches concerning the soybean technology were developed at ICCPT Fundulea and ARDS Turda, but also at other agricultural research stations in the country, but they were few in the pedoclimatic area of Central Moldavia. Thus, under the A.R.D.S. Secuieni pedoclimatic conditions were performed researches at soybean crop in order to establish the optimum sowing epoch (three sowing epochs: early sowing - 04/09/2015, sowing in the optimum epoch - 20/04/2015 and sowing in the late epoch - 04/30/2015), the optimal seeding density (six sowing densities: 30 g.b./sqm, 40 g.b./sqm, 50 g.b./sqm, 60 g.b./sqm, 70 g.b./sqm and 80 g.b./sqm) and the best distance between rows (four distances: 12.5 cm, 25 cm, 37.5 cm and 50 cm). The biological materials used in these experiments were the Eugen and Onyx varieties, created at A.R.D.S. Turda. The results obtained in 2015 agricultural year, which is several droughty characterized, showed that sowing soybean in late epoch was the best option, the yields obtained in these variants reach 2653 kg/ha at the Eugen variety and 3244 kg/ha at the Onix variety. Regarding the sowing density, the 30 g.b./sqm and the 80 g.b./sqm sown variant were remarked as being the best. The yields obtained in this variants were at Eugen variety of 2994 kg/ha when the variety was sown with 30 g.b./sqm and of 3142 kg/ha when this variety was sown with 80 g.b./sqm. The Onix variety has obtained the maximum yield in the variant sown with 30 g.b./sqm of 3041 kg/ha, and in the variant sown with 80 g.b./sqm, the yield was slightly smaller but big enough of 2937 kg/ha. Given the price of soybean seed, the most convenient variant from the economic point of view is the one sown with 30 g.b./sqm, both at Eugen and Onix variety. The distance between rows also had an influence on the soybean yield. Of the four experimented distances tested, it was proven to be superior to all others, the one with 50 cm between rows. In this variant, the yields obtained amounted to 2530 kg/ha at Eugen variety and 2715 kg/ha Onix variety

Sorghum bicolor (L.) Moench. - Plant for the future in Moldavia agriculture (Romania) and for the human nutrition

In the climatic conditions of the Center of Moldavia (Romania), there are eight sorghum hybrids cultivated for grains (Arsky, Foehn, Albanus, Shamal, Kalatur şi Armorik, Elan and Alimentar) in order to establish their adaptability to the conditions of the area, by taking into account the current problems of agriculture caused by climate change and the need to find a solutions to fight hunger and improve human health. This study focused on two directions, namely: identifying genotypes that achieve the highest productions and establishing food value and the benefits of sorghum grains on human health by analysing the physico chemical composition of the grains, by determining the mineral content of the grain and by determining the composition in amino acids. The grain production had ranged from 8623 kg/ha to 11181 kg/ha. This production had characterized by a protein content between 8.84 % and 12.80 %, in lipid content between 3.39 % and 4.15 %, in raw fibre content between 2.15 % and 3.95 % and the starch content was between 66.70 % and 74.66 %. The analysed mineral content of the sorghum samples has the following values (mg/100g a.s.): phosphorus - 280 - 330; potassium - 520 - 610; calcium - 2.4 - 3.9; magnesium - 260 - 290; iron - 11.6 - 18.9; zinc - 1.94 - 2.42; copper - 0.23 - 0.36; manganese - 1.60 - 1.97. Analysing the amino acid composition of sorghum samples it can be said that the samples contain all the essential amino acids, in concentrations that, some of them, exceed the recommended daily dose (FAO/WHO/UNU): leucine, isoleucine, histidine, valine. Also the analysed sorghum seeds add an important content of methionine, threonine and phenylalanine

Antibody and Nanobody Radiolabeling with Copper-64: Solid vs. Liquid Target Approach

Antibody and nanobody-based copper-64 radiopharmaceuticals are increasingly being proposed as theranostic tools in multiple human diseases. While the production of copper-64 using solid targets has been established for many years, its use is limited due to the complexity of solid target systems, which are available in only a few cyclotrons worldwide. In contrast, liquid targets, available in virtually in all cyclotrons, constitute a practical and reliable alternative. In this study, we discuss the production, purification, and radiolabeling of antibodies and nanobodies using copper-64 obtained from both solid and liquid targets. Copper-64 production from solid targets was performed on a TR-19 cyclotron with an energy of 11.7 MeV, while liquid target production was obtained by bombarding a nickel-64 solution using an IBA Cyclone Kiube cyclotron with 16.9 MeV on target. Copper-64 was purified from both solid and liquid targets and used to radiolabel NODAGA-Nb, NOTA-Nb, and DOTA-Trastuzumab conjugates. Stability studies were conducted on all radioimmunoconjugates in mouse serum, PBS, and DTPA. Irradiation of the solid target yielded 13.5 ± 0.5 GBq with a beam current of 25 ± 1.2 μA and an irradiation time of 6 h. On the other hand, irradiation of the liquid target resulted in 2.8 ± 1.3 GBq at the end of bombardment (EOB) with a beam current of 54.5 ± 7.8 μA and an irradiation time of 4.1 ± 1.3 h. Successful radiolabeling of NODAGA-Nb, NOTA-Nb, and DOTA-Trastuzumab with copper-64 from both solid and liquid targets was achieved. Specific activities (SA) obtained with the solid target were 0.11, 0.19, and 0.33 MBq/μg for NODAGA-Nb, NOTA-Nb, and DOTA-trastuzumab, respectively. For the liquid target, the corresponding SA values were 0.15, 0.12, and 0.30 MBq/μg. Furthermore, all three radiopharmaceuticals demonstrated stability under the testing conditions. While solid targets have the potential to produce significantly higher activity in a single run, the liquid process offers advantages such as speed, ease of automation, and the feasibility of back-to-back production using a medical cyclotron. In this study, successful radiolabeling of antibodies and nanobodies was achieved using both solid and liquid targets approaches. The radiolabeled compounds exhibited high radiochemical purity and specific activity, rendering them suitable for subsequent in vivo pre-clinical imaging studies

Predicting the Early Response to Neoadjuvant Therapy with Breast MR Morphological, Functional and Relaxometry Features—A Pilot Study

Aim: To evaluate the role of MR relaxometry and derived proton density analysis in the prediction of early treatment response after two cycles of neoadjuvant therapy (NAT), in patients with breast cancer. Methods: This was a prospective study that included 59 patients with breast cancer, who underwent breast MRI prior (MRI1) and after two cycles of NAT (MRI2). The MRI1 included a sequential acquisition with five different TE’s (50, 100, 150, 200 and 250 ms) and a TR of 5000 ms. Post-processing was used to obtain the T2 relaxometry map from the MR acquisition. The tumor was delineated and seven relaxometry and proton density parameters were extracted. Additional histopathology data, T2 features and ADC were included. The response to NAT was reported based on the MRI2 as responders: partial response (>30% decreased size) and complete response (no visible tumor stable disease (SD); and non-responders: stable disease or progression (>20% increased size). Statistics was done using Medcalc software. Results: There were 50 (79.3%) patients with response and 13 (20.7%) non-responders to NAT. Age, histologic type, “in situ” component, tumor grade, estrogen and progesterone receptors, ki67% proliferation index and HER2 status were not associated with NAT response (all p > 0.05). The nodal status (N) 0 was associated with early response, while N2 was associated with non-response (p = 0.005). The tumor (T) and metastatic (M) stage were not statistically significant associated with response (p > 0.05). The margins, size and ADC values were not associated with NAT response (p-value > 0.05). The T2 min relaxometry value was associated with response (p = 0.017); a cut-off value of 53.58 obtained 86% sensitivity (95% CI 73.3–94.2), 69.23 specificity (95% CI 38.6–90.9), with an AUC = 0.715 (p = 0.038). The combined model (T2 min and N stage) achieved an AUC of 0.826 [95% CI: 0.66–0.90, p-value < 0.001]. Conclusions: MR relaxometry may be a useful tool in predicting early treatment response to NAT in breast cancer patients