Beskrywing van tegnieke en chemiese analises van skaapliggame en foute daaraan verbonde

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Characterization and Comparison of Convergence Among \u3cem\u3eCephalotus follicularis\u3c/em\u3e Pitcher Plant-Associated Communities with Those of \u3cem\u3eNepenthes\u3c/em\u3e and \u3cem\u3eSarracenia\u3c/em\u3e Found Worldwide

The Albany pitcher plant, Cephalotus follicularis, has evolved cup-shaped leaves and a carnivorous habit completely independently from other lineages of pitcher plants. It is the only species in the family Cephalotaceae and is restricted to a small region of Western Australia. Here, we used metabarcoding to characterize the bacterial and eukaryotic communities living in C. follicularis pitchers at two different sites. Bacterial and eukaryotic communities were correlated in both richness and composition; however, the factors associated with richness were not the same across bacteria and eukaryotes, with bacterial richness differing with fluid color, and eukaryotic richness differing with the concentration of DNA extracted from the fluid, a measure roughly related to biomass. For turnover in composition, the variation in both bacterial and eukaryotic communities primarily differed with fluid acidity, fluid color, and sampling site. We compared C. follicularis-associated community diversity with that of Australian Nepenthes mirabilis, as well as a global comparison of Southeast Asian Nepenthes and North American Sarracenia. Our results showed similarity in richness with communities from other pitcher plants, and specific bacterial taxa shared among all three independent lineages of pitcher plants. Overall, we saw convergence in richness and particular clades colonizing pitcher plants around the world, suggesting that these highly specialized habitats select for certain numbers and types of inhabitants

Taller de Química experimental: una mirada más allá del tubo de ensayo

El taller consiste en una propuesta de trabajo grupal entre los alumnos y el coordinador docente en el que se emplean las metodologías que ayuden a aprender a pensar, aplicar conocimientos previamente adquiridos y descubrir nuevos. Es una propuesta abierta que conjuga trabajo con creatividad y cierta dosis lúdica, haciendo de la clase una experiencia activa donde se construye, intercambia y recrea con espacio para imaginar, equivocarse, ensayar, reflexionar. Así se desarrolló un taller optativo de Química Experimental con alumnos del 6º año del Ciclo ESS orientado en Ciencias Naturales. Se buscaron actividades donde los alumnos desarrollaran aspectos creativos sin perder de vista la adquisición y afianzamiento de conceptos abordados total o parcialmente en distintas asignaturas. Los temas seleccionados fueron: Metales y Corrosión; Colorantes: estructura e interacción con la luz, su obtención a partir de líquenes; Vitaminas: estructura y propiedades; Elaboración de productos cosméticos (jabones, champú, cremas); Esencias: obtención; Trabajo con polímeros sintéticos. El trabajo permitió sacar conclusiones críticas y enriquecedoras para alumnos y docentes.Trabajos del área Ciencias NaturalesDepartamento de Ciencias Exactas y Naturale

Distinct neurobehavioural effects of cannabidiol in transmembrane domain neuregulin 1 mutant mice

The cannabis constituent cannabidiol (CBD) possesses anxiolytic and antipsychotic properties. We have previously shown that transmembrane domain neuregulin 1 mutant (Nrg1 TM HET) mice display altered neurobehavioural responses to the main psychoactive constituent of cannabis, D9-tetrahydrocannabinol. Here we investigated whether Nrg1 TM HET mice respond differently to CBD and whether CBD reverses schizophrenia-related phenotypes expressed by these mice. Adult male Nrg1 TM HET and wild type-like littermates (WT) received vehicle or CBD (1, 50 or 100 mg/kg i.p.) for 21 days. During treatment and 48 h after withdrawal we measured behaviour, whole blood CBD concentrations and autoradiographic receptor binding. Nrg1 HET mice displayed locomotor hyperactivity, PPI deficits and reduced 5-HT2A receptor binding density in the substantia nigra, but these phenotypes were not reversed by CBD. However, long-term CBD (50 and 100 mg/ kg) selectively enhanced social interaction in Nrg1 TM HET mice. Furthermore, acute CBD (100 mg/kg) selectively increased PPI in Nrg1 TM HET mice, although tolerance to this effect was manifest upon repeated CBD administration. Long-term CBD (50 mg/kg) also selectively increased GABAA receptor binding in the granular retrosplenial cortex in Nrg1 TM HET mice and reduced 5-HT2A binding in the substantia nigra in WT mice. Nrg1 appears necessary for CBD-induced anxiolysis since only WT mice developed decreased anxiety-related behaviour with repeated CBD treatment. Altered pharmacokinetics in mutant mice could not explain our findings since no genotype differences existed in CBD blood concentrations. Here we demonstrate that Nrg1 modulates acute and long-term neurobehavioural effects of CBD, which does not reverse the schizophrenia-relevant phenotypes

Novel molecular changes induced by Nrg1 hypomorphism and Nrg1-cannabinoid interaction in adolescence : a hippocampal proteomic study in mice

Neuregulin 1 (NRG1) is linked to an increased risk of developing schizophrenia and cannabis dependence. Mice that are hypomorphic for Nrg1 (Nrg1 HET mice) display schizophrenia relevant behavioural phenotypes and aberrant expression of serotonin and glutamate receptors. Nrg1 HET mice also display idiosyncratic responses to the main psychoactive constituent of cannabis, Δ9 -tetrahydrocannabinol (THC). To gain traction on the molecular pathways disrupted by Nrg1 hypomorphism and Nrg1-cannabinoid interactions we conducted a proteomic study. Adolescent wildtype (WT) and Nrg1 HET mice were exposed to repeated injections of vehicle or THC and their hippocampi were submitted to 2D gel proteomics. Comparison of WT and Nrg1 HET mice identified proteins linked to molecular changes in schizophrenia that have not been previously associated with Nrg1. These proteins are involved in vesicular release of neurotransmitters such as SNARE proteins; enzymes impacting serotonergic neurotransmission, and; proteins affecting growth factor expression. Nrg1 HET mice treated with THC expressed a distinct protein expression signature compared to WT mice. Replicating prior findings, THC caused proteomic changes in WT mice suggestive of greater oxidative stress and neurodegeneration. We have previously observed that THC selectively increased hippocampal NMDA receptor binding of adolescent Nrg1 HET mice. Here we observed outcomes consistent with heightened NMDA-mediated glutamatergic neurotransmission. This included differential expression of proteins involved in NMDA receptor trafficking to the synaptic membrane; lipid raft stabilization of synaptic NMDA receptors; and homeostatic responses to dampen excitotoxicity. These findings uncover for the first time novel proteins altered in response to Nrg1 hypomorphism and Nrg1-cannabinoid interactions that improves our molecular understanding of Nrg1 signaling and Nrg1-mediated genetic vulnerability to the neurobehavioural effects of cannabinoids

Investigación de actividad amebicida del producto comercial ‘Espadol Dettol Solución, antiséptico’ sobre quistes y trofozoitos de Acanthamoeba spp.

Evaluar la actividad amebicida in vitro del producto comercial ‘Espadol Dettol, solución antiséptico’ (principio activo: cloroxilenol), sobre una suspensión de quistes y trofozoítos de Acanthamoeba spp. con distintas concentraciones del mismo y a diferentes tiempos de incubación.Facultad de Ciencias Veterinaria

Investigación de actividad amebicida del producto comercial ‘Espadol Dettol Solución, antiséptico’ sobre quistes y trofozoitos de Acanthamoeba spp.

Evaluar la actividad amebicida in vitro del producto comercial ‘Espadol Dettol, solución antiséptico’ (principio activo: cloroxilenol), sobre una suspensión de quistes y trofozoítos de Acanthamoeba spp. con distintas concentraciones del mismo y a diferentes tiempos de incubación.Facultad de Ciencias Veterinaria

Investigación de actividad amebicida del producto comercial ‘Espadol Dettol Solución, antiséptico’ sobre quistes y trofozoitos de Acanthamoeba spp.

Evaluar la actividad amebicida in vitro del producto comercial ‘Espadol Dettol, solución antiséptico’ (principio activo: cloroxilenol), sobre una suspensión de quistes y trofozoítos de Acanthamoeba spp. con distintas concentraciones del mismo y a diferentes tiempos de incubación.Facultad de Ciencias Veterinaria