Diethyl 4-(6-Chloroimidazo[2,1-b]thiazol-5-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate and Ethyl 4-(6-Chloroimidazo[2,1-b]thiazol-5-yl)-6-methyl-2-oxo-1,2,3,4-tetrahydropyrimidine-5-carboxylate

Diethyl 4-(6-chloroimidazo[2,1-b]thiazol-5-yl)-2,6-dimethyl-1,4-dihydropyridine- 3,5-dicarboxylate and ethyl 4-(6-chloroimidazo[2,1-b]thiazol-5-yl)-6-methyl-2-oxo-1,2,3,4-tetrahydropyrimidine-5-carboxylate were obtained simultaneously by the Biginelli reaction using a green protocol and curtailing reaction time

Assessment of seismic vulnerability of historical defensive walls

This paper presents a study on the behaviour of the walls of the Rocca Roveresca of Senigallia in Italy built in the XIV century on the ruins of a former Roman defensive struc-ture. This is a peculiar example of a small fortress that had undergone in the XV century im-portant modifications of the plant in order to enhance its defensive performances. A linear finite element model is first developed in order to understand the dynamic behaviour of a ge-neric portion of the wall and to detect the probable incipient failure mechanisms. A subse-quent static nonlinear analysis is carried out, with the same finite element model, to investigate the formation of the cracking layout and to detect the position of plastic hinges. The last analysis level is carried out with a tailored macro-element constituted by three bod-ies, namely the two external curtains and the inner fill for which a degradation of the behav-iour is considered. The results obtained demonstrates the efficiency of the wall against earthquakes characterized by return times typical for ultimate limit states. Same issues that deserve further investigation are highlighted

Potentially Toxic Elements (PTEs) in Cultivated Soils from Lombardy (Northern Italy): Spatial Distribution, Origin, and Management Implications

This work aims to quantify the contribution of agricultural practices to diffuse pollution in cultivated soils. Two samples (the first from the top layer and the second from a depth of about 100 cm) were retrieved from about 1000 soil profiles and analyzed for their potentially toxic elements (PTEs) content (Cd, Cu, Ni, Pb, Zn, Mn). In addition, we collected dedicated soil profiles for three specific types of land use and agronomic practice, namely vine-, cereal- and rice-growing areas. Baseline concentrations and distribution maps were produced. Statistical data treatment, coupled with the results of the dedicated soil profiles, enabled the identification of precise pollution sources and processes. In cultivated soils, PTEs contents prove to be generally well below the Italian regulatory limits, with the exception of Cu in vineyard soils, due to the long-lasting use of Cu-based pesticides. South of Milan and in other urban areas, we attribute the top soil enrichment in Cd, Cu, Pb, and Zn to industrial activities and urban settlement. Ni sporadically exceeds regulatory standards, but its association with Mn and its depth distribution point to a natural origin, mostly due to the occurrence of serpentine. The project demonstrates that although agriculture, especially in the form of vineyards and of intensive cultivation, certainly contributes to pollution, such contribution is exceeded by that deriving from industry and road traffic

Rockfall susceptibility analysis through 3D simulations in marine protected areas of the Portofino coastline: case studies of San Fruttuoso and Paraggi bays

The research focuses on the assessment of the potential geomorphological hazards affecting the stability of the Promontory of Portofino (Regional Natural Park of Portofino, Northern Italy), mainly on the bays of San Fruttuoso and Paraggi. The study area constitutes one of the most representative and most populated environments, both by locals and tourists, of the entire Liguria area. For this reason, there is a growing need for investigation on the natural dynamics of these landscapes, based on detailed topographic information. The research starts from a regional geological and geomorphological analysis specific to the study area, combined with a multi-model comparison algorithm (M3C2), which allows comparing two LiDAR datasets acquired in 2008 and 2020, respectively, to assess the overall dynamics of the promontory development. Then, a detailed study of San Fruttuoso and Paraggi bays, two key points for visitors and therefore for management, is performed. Three-dimensional modeling of rockfalls is carried out, which allows the development of a specific management oriented to the use of nature-based solutions (NBSs) strategies, respecting the environment and increasing safety against instability processes in these areas.This study has been carried out in the framework of a collaboration among ISPRA, GISIG, Universita di Genova, and Universidad del Pais Vasco (UPV/EHU, IT1678-22 Research Group, PES-18/92 Project) within the RECONECT Project (Regenerating ECOsystems with Nature-based solutions for hydro-meteorological risk rEduCTion)

Synthesis, in vitro and in vivo biological evaluation of substituted 3-(5-imidazo[2,1-b]thiazolylmethylene)-2-indolinones as new potent anticancer agents

A small library of 3-(5-imidazo[2,1-b]thiazolylmethylene)-2-indolinones has been synthesized and screened according to protocols available at the National Cancer Institute (NCI). Some derivatives were potent antiproliferative agents, showing GI50 values in the nanomolar range. Remarkably, when most active compounds against leukemia cells were tested in human peripheral blood lymphocytes from healthy donors, were 100–200 times less cytotoxic. Some compounds, selected by the Biological Evaluation Committee of NCI, were examined to determine tubulin assembly inhibition. Furthermore, flow cytometric studies performed on HeLa, HT-29, and A549 cells, showed that compounds 14 and 25 caused a block in the G2/M phase. Interestingly, these derivatives induced apoptosis through the mitochondrial death pathway, causing in parallel significant activation of both caspase-3 and -9, PARP cleavage and down-regulation of the anti-apoptotic proteins Bcl-2 and Mcl-1. Finally, compound 25 was also tested in vivo in the murine BL6-B16 melanoma and E0771 breast cancer cells, causing in both cases a significant reduction in tumor volume

Risk assessment and prevention priorities in cultural heritage preservation

European Union has been promoting research actions on cultural heritage, recognizing and underlining its central role for the community policies and establishing its safeguard and valorisation as urgent priorities for the future. A research on rational tools for establishing seismic risk, intervention priorities, and decision-making on renovation of historical buildings and museums, just started at the University of Camerino, School of Architecture and Design, is described in this paper. The basic idea of the research is to develop a probabilistic methodology for the assessment of seismic risk of cultural Heritage starting from the Pacific Earthquake Engineering Research (PEER) approach, consisting of a general framework where the risk problem is decomposed into its three main features (i.e. seismic hazard, vulnerability and losses), analysed in a rigorous and consistent interdependent manner. The application of this methodology to cultural heritage requires investigations and original proposals on various open issues. This paper reports some results concerning the general methodology and preliminary analyses of a case study

Haplotype affinities resolve a major component of goat (<i>Capra hircus</i>) MtDNA D-loop diversity and reveal specific features of the Sardinian stock

Goat mtDNA haplogroup A is a poorly resolved lineage absorbing most of the overall diversity and is found in locations as distant as Eastern Asia and Southern Africa. Its phylogenetic dissection would cast light on an important portion of the spread of goat breeding. The aims of this work were 1) to provide an operational definition of meaningful mtDNA units within haplogroup A, 2) to investigate the mechanisms underlying the maintenance of diversity by considering the modes of selection operated by breeders and 3) to identify the peculiarities of Sardinian mtDNA types. We sequenced the mtDNA D-loop in a large sample of animals (1,591) which represents a non-trivial quota of the entire goat population of Sardinia. We found that Sardinia mirrors a large quota of mtDNA diversity of Western Eurasia in the number of variable sites, their mutational pattern and allele frequency. By using Bayesian analysis, a distance-based tree and a network analysis, we recognized demographically coherent groups of sequences identified by particular subsets of the variable positions. The results showed that this assignment system could be reproduced in other studies, capturing the greatest part of haplotype diversity. We identified haplotype groups overrepresented in Sardinian goats as a result of founder effects. We found that breeders maintain diversity of matrilines most likely through equalization of the reproductive potential. Moreover, the relevant amount of inter-farm mtDNA diversity found does not increase proportionally with distance. Our results illustrate the effects of breeding practices on the composition of maternal gene pool and identify mtDNA types that may be considered in projects aimed at retrieving the maternal component of the oldest breeds of Sardinia.</br

Water planning and management in the Cornia river plain by means of the gis-integrated freewat platform

Coastal aquifer, Cornia valley, Tuscany, Managed Aquifer Recharge, FREEWAT platform