137 research outputs found
Samenhang in de maatschappelijke dienstverlening: Een onderzoek naar de ontwikkeling van hulpverleningsnetwerken in tien Nederlandse gemeenten
Transferring an optimized TAP-toolbox for the isolation of protein complexes to a portfolio of rice tissues
Dust in the Small Magellanic Cloud: Interstellar Polarization and Extinction
To elucidate the dust properties in the SMC we have for the first time
measured linear polarization in five colors in the optical region of the
spectrum for a sample of reddened stars. For two of these stars, for which
there were no existing UV spectrophotometric measurements, but for which we
measured a relatively large polarization, we have also obtained data from the
International Ultraviolet Explorer (IUE) in order to study the extinction. The
main results are: (1) the wavelength of maximum polarization, ,
in the SMC is typically smaller than that in the Galaxy; (2) however, AZV 456,
which shows the UV extinction bump, has a typical of that in
the Galaxy, its polarization curve is narrower, its bump is shifted to shorter
wavelengths as compared to the Galaxy and its UV extinction does not conform to
the Galactic analytical interpolation curve based on the ratio of total to
selective extinction; (3) the 'typical', monotonic SMC extinction curve can be
best fit with amorphous carbon and silicate grains; (4) the extinction towards
AZV456 may only be explained by assuming a larger gas-to-dust ratio than the
observed N(HI)/A(V) value, with a small amount of the available carbon in
graphite form; (5) from an analysis of both the extinction and polarization
data and our model fits it appears that the SMC has typically smaller grains
than those in the Galaxy.Comment: To appear in the ApJ, 50 pages, latex fil
A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth
[EN] Adverse environmental conditions trigger responses in plants that promote stress tolerance and survival at the expense of growth(1). However, little is known of how stress signalling pathways interact with each other and with growth regulatory components to balance growth and stress responses. Here, we show that plant growth is largely regulated by the interplay between the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1) protein kinase and the abscisic acid (ABA) phytohormone pathway. While SnRK2 kinases are main drivers of ABA-triggered stress responses, we uncover an unexpected growth-promoting function of these kinases in the absence of ABA as repressors of SnRK1. Sequestration of SnRK1 by SnRK2-containing complexes inhibits SnRK1 signalling, thereby allowing target of rapamycin (TOR) activity and growth under optimal conditions. On the other hand, these complexes are essential for releasing and activating SnRK1 in response to ABA, leading to the inhibition of TOR and growth under stress. This dual regulation of SnRK1 by SnRK2 kinases couples growth control with environmental factors typical for the terrestrial habitat and is likely to have been critical for the water-to-land transition of plants.We thank J.-K. Zhu for the snrk2 mutants, M. Bennett for the SnRK2.2-GFP line, C. Koncz for the SnRK1-GFP line, X. Li for the SnRK2.3-FLAG OE line, J. Schroeder for the GFP-His-FLAG and SnRK2.6-His-FLAG OE lines, C. Mackintosh for the TPS5 antibody and the Nottingham Arabidopsis stock centre for T-DNA mutant seeds. The IGC Plant Facility (Vera Nunes) is thanked for excellent plant care. This work was supported by Fundacao para a Ciencia e a Tecnologia through the R&D Units UIDB/04551/2020 (GREEN-IT-Bioresources for Sustainability) and UID/MAR/04292/2019, FCT project nos. PTDC/BIA-PLA/7143/2014, LISBOA-01-0145-FEDER-028128 and PTDC/BIA-BID/32347/2017, and FCT fellowships/contract nos. SFRH/BD/122736/2016 (M.A.), SFRH/BPD/109336/2015 (A.C.), PD/BD/150239/2019 (D.R.B.), and IF/00804/2013 (E.B.G.). Work in P.L.R.'s laboratory was funded by MCIU grant no. BIO2017-82503-R. C.M. thanks the LabEx Paris Saclay Plant Sciences-SPS (ANR-10-LABX-040-SPS) for support. B.B.P. was funded by Programa VALi+d GVA APOSTD/2017/039. This project has received funding from the European Union Horizon 2020 research and innovation programme (grant agreement no. 867426-ABA-GrowthBalance-H2020-WF-2018-2020/H2020-WF-01-2018, awarded to B.B.P.). This work is dedicated to the memory of our beloved friend and colleague Americo Rodrigues.Belda-Palazón, B.; Adamo, M.; Valerio, C.; Ferreira, LJ.; Confraria, A.; Reis-Barata, D.; Rodrigues, A.... (2020). A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth. 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Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice
Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAP-tagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 TAP-tagged rice protein kinases with a concurrent screen using yeast two hybrid methods identified an evolutionarily new rice protein that interacts with the well conserved cell division cycle 2 (CDC2) protein complex
The phylogenomic analysis of the anaphase promoting complex and its targets points to complex and modern-like control of the cell cycle in the last common ancestor of eukaryotes
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