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Experiencing ‘Otherness’ in Ethnic-Themed Restaurants
Ethnic-themed restaurants are one of the most powerful influential socializing agents of foreign cultures. The most common reference point for those seeking enriching leisurely cultural experience is object authenticity. Yet little research has been done on authenticity in eatertainment experiences from both supply and demand perspectives. This paper attempts to fill the lacuna through exploring how ethnic-themed restaurant owners/managers use markers to create a genuine version of their food and cook in the virtual cyber world and how patrons project authenticity to their experiences on the blogosphere. The preliminary results indicate that patrons continue to place emphasis on experiencing the \u27other\u27 in an objectively authentic setting but in a negotiated manner so that it relates to their comfort and style. More effort is needed on the part of management to understand consumer demand and \u27typify the market niche\u27
Supporting Community Health Workers in India through Voice- and Web-Based Feedback
Our research aims to support community health workers (CHWs) in low-resource settings by providing them with personalized information regarding their work. This information is delivered through a combination of voice- and web-based feedback that is derived from data already collected by CHWs. We describe the in situ participatory design approach used to create usable and appropriate feedback for low-literate CHWs and present usage data from a 12-month study with 71 CHWs in India. We show how the system supported and motivated CHWs, and how they used both the web- and voice-based systems, and each of the visualizations, for different reasons. We also show that the comparative feedback provided by the system introduced elements of competition that discouraged some CHWs while motivating others. Taken together, our findings suggest that providing personalized voice- and web-based feedback could be an effective way to support and motivate CHWs in low-resource settings
Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC
Nucleotides and nucleosides are not only involved in cellular metabolism but also act extracellularly via P1 and P2 receptors, to elicit a wide variety of physiological and pathophysiological responses through paracrine and autocrine signalling pathways. For the first time, we have used an ion-pair reversed-phase high-performance liquid chromatography ultraviolet (UV)-coupled method to rapidly and simultaneously quantify 12 different nucleotides and nucleosides (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, adenosine, uridine triphosphate, uridine diphosphate, uridine monophosphate, uridine, guanosine triphosphate, guanosine diphosphate, guanosine monophosphate, guanosine): (1) released from a mouse renal cell line (M1 cortical collecting duct) and (2) in human biological samples (i.e., urine). To facilitate analysis of urine samples, a solid-phase extraction step was incorporated (overall recovery rate ? 98 %). All samples were analyzed following injection (100 ?l) into a Synergi Polar-RP 80 Å (250 × 4.6 mm) reversed-phase column with a particle size of 10 ?m, protected with a guard column. A gradient elution profile was run with a mobile phase (phosphate buffer plus ion-pairing agent tetrabutylammonium hydrogen sulfate; pH 6) in 2-30 % acetonitrile (v/v) for 35 min (including equilibration time) at 1 ml min(-1) flow rate. Eluted compounds were detected by UV absorbance at 254 nm and quantified using standard curves for nucleotide and nucleoside mixtures of known concentration. Following validation (specificity, linearity, limits of detection and quantitation, system precision, accuracy, and intermediate precision parameters), this protocol was successfully and reproducibly used to quantify picomolar to nanomolar concentrations of nucleosides and nucleotides in isotonic and hypotonic cell buffers that transiently bathed M1 cells, and urine samples from normal subjects and overactive bladder patients
Searching for Exoplanets Using a Microresonator Astrocomb
Detection of weak radial velocity shifts of host stars induced by orbiting
planets is an important technique for discovering and characterizing planets
beyond our solar system. Optical frequency combs enable calibration of stellar
radial velocity shifts at levels required for detection of Earth analogs. A new
chip-based device, the Kerr soliton microcomb, has properties ideal for
ubiquitous application outside the lab and even in future space-borne
instruments. Moreover, microcomb spectra are ideally suited for astronomical
spectrograph calibration and eliminate filtering steps required by conventional
mode-locked-laser frequency combs. Here, for the calibration of astronomical
spectrographs, we demonstrate an atomic/molecular line-referenced,
near-infrared soliton microcomb. Efforts to search for the known exoplanet HD
187123b were conducted at the Keck-II telescope as a first in-the-field
demonstration of microcombs
ERP Conceptual Ecology
The technological evolution of recent years has made that information systems frequently adapt to the market realities to fulfill the improvements of the company’s organizational processes. In this context, new
paradigms, approaches, and concepts were disseminated through the new realities of information systems. This study aims to verify how ERP (Enterprise Resource Planning) has been related to other information systems within its ecosystem. For this purpose, we have reviewed the literature based on 650
publications whose central theme was the ERP. The data were treated through a graphical analysis, inspired by SNA (Social Network Analysis), represented by related ERP concepts. The study results, determine the connection degree between the concepts that emerged with the technological evolution and the
ERP, thus representing the ERP interoperability tendencies, over the last years.
The study concludes that ERPs have been improving and substantially increasing the conditions of
nteroperability with other information systems and with new organizational concepts that have emerged through the technological availability. This fact led to a better organizational process’s adoption and more
organizational performance.info:eu-repo/semantics/publishedVersio
Decontamination of MDA Reagents for Single Cell Whole Genome Amplification
Single cell genomics is a powerful and increasingly popular tool for studying the genetic make-up of uncultured microbes. A key challenge for successful single cell sequencing and analysis is the removal of exogenous DNA from whole genome amplification reagents. We found that UV irradiation of the multiple displacement amplification (MDA) reagents, including the Phi29 polymerase and random hexamer primers, effectively eliminates the amplification of contaminating DNA. The methodology is quick, simple, and highly effective, thus significantly improving whole genome amplification from single cells
A Measurement of Rb using a Double Tagging Method
The fraction of Z to bbbar events in hadronic Z decays has been measured by
the OPAL experiment using the data collected at LEP between 1992 and 1995. The
Z to bbbar decays were tagged using displaced secondary vertices, and high
momentum electrons and muons. Systematic uncertainties were reduced by
measuring the b-tagging efficiency using a double tagging technique. Efficiency
correlations between opposite hemispheres of an event are small, and are well
understood through comparisons between real and simulated data samples. A value
of Rb = 0.2178 +- 0.0011 +- 0.0013 was obtained, where the first error is
statistical and the second systematic. The uncertainty on Rc, the fraction of Z
to ccbar events in hadronic Z decays, is not included in the errors. The
dependence on Rc is Delta(Rb)/Rb = -0.056*Delta(Rc)/Rc where Delta(Rc) is the
deviation of Rc from the value 0.172 predicted by the Standard Model. The
result for Rb agrees with the value of 0.2155 +- 0.0003 predicted by the
Standard Model.Comment: 42 pages, LaTeX, 14 eps figures included, submitted to European
Physical Journal
Expanding the Versatility of Phage Display I: Efficient Display of Peptide-Tags on Protein VII of the Filamentous Phage
Background: Phage display is a platform for selection of specific binding molecules and this is a clear-cut motivation for increasing its performance. Polypeptides are normally displayed as fusions to the major coat protein VIII (pVIII), or the minor coat protein III (pIII). Display on other coat proteins such as pVII allows for display of heterologous peptide sequences on the virions in addition to those displayed on pIII and pVIII. In addition, pVII display is an alternative to pIII or pVIII display.
Methodology/Principal Findings: Here we demonstrate how standard pIII or pVIII display phagemids are complemented with a helper phage which supports production of virions that are tagged with octa FLAG, HIS6 or AviTag on pVII. The periplasmic signal sequence required for pIII and pVIII display, and which has been added to pVII in earlier studies, is omitted altogether.
Conclusions/Significance: Tagging on pVII is an important and very useful add-on feature to standard pIII and pVII display. Any phagemid bearing a protein of interest on either pIII or pVIII can be tagged with any of the tags depending simply on choice of helper phage. We show in this paper how such tags may be utilized for immobilization and separation as well as purification and detection of monoclonal and polyclonal phage populations.
© 2011 Wälchli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells
TET proteins oxidize 5-methylcytosine in DNA to 5-hydroxymethylcytosine and other oxidation products. We found that simultaneous deletion of Tet2 and Tet3 in mouse CD4+CD8+ double-positive thymocytes resulted in dysregulated development and proliferation of invariant natural killer T cells (iNKT cells). Tet2-Tet3 double-knockout (DKO) iNKT cells displayed pronounced skewing toward the NKT17 lineage, with increased DNA methylation and impaired expression of genes encoding the key lineage-specifying factors T-bet and ThPOK. Transfer of purified Tet2-Tet3 DKO iNKT cells into immunocompetent recipient mice resulted in an uncontrolled expansion that was dependent on the nonclassical major histocompatibility complex (MHC) protein CD1d, which presents lipid antigens to iNKT cells. Our data indicate that TET proteins regulate iNKT cell fate by ensuring their proper development and maturation and by suppressing aberrant proliferation mediated by the T cell antigen receptor (TCR)
Measurement of the B+ and B-0 lifetimes and search for CP(T) violation using reconstructed secondary vertices
The lifetimes of the B+ and B-0 mesons, and their ratio, have been measured in the OPAL experiment using 2.4 million hadronic Z(0) decays recorded at LEP. Z(0) --> b (b) over bar decays were tagged using displaced secondary vertices and high momentum electrons and muons. The lifetimes were then measured using well-reconstructed charged and neutral secondary vertices selected in this tagged data sample. The results aretau(B+) = 1.643 +/- 0.037 +/- 0.025 pstau(Bo) = 1.523 +/- 0.057 +/- 0.053 pstau(B+)/tau(Bo) = 1.079 +/- 0.064 +/- 0.041,where in each case the first error is statistical and the second systematic.A larger data sample of 3.1 million hadronic Z(o) decays has been used to search for CP and CPT violating effects by comparison of inclusive b and (b) over bar hadron decays, No evidence fur such effects is seen. The CP violation parameter Re(epsilon(B)) is measured to be Re(epsilon(B)) = 0.001 +/- 0.014 +/- 0.003and the fractional difference between b and (b) over bar hadron lifetimes is measured to(Delta tau/tau)(b) = tau(b hadron) - tau((b) over bar hadron)/tau(average) = -0.001 +/- 0.012 +/- 0.008
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