Representations of Class, Social Realism and Region in Eleven Months in Bunbury by James Ricks

The aim of this thesis is to explore representations of class, social realism and region in Eleven months in Bunbury by James Ricks. This novel stands outside dominant literary theory in its representations of class, realism and regionalism. It also presents opportunities to consider ideology and class through the eyes of a working class person, in the language of the class that it depicts. Thus it speaks to a class which rarely has its point of view and lives represented in conventional literature. It is therefore a useful literary and social document

Reading Aloud: Children\u27s Attitudes toward being Read to at Home and at School

: Whilst there exists a plethora of research about the benefits of reading aloud on children’s literacy development and a range of government reports highlighting the positive investment return on early intervention strategies such as reading aloud, most literature is presented from an adult perspective. Limited research exists on children’s attitudes toward being read to at home or school or the frequency of reading aloud practices that occur within these contexts. This mixed method study examines reading aloud practices in schools (N=21) and homes (N=220). It captures the attitude toward reading aloud practices from the viewpoint of 220 children aged between 6-12 years of age (Grade 1-6) across a representative range of diverse school contexts in Western Australian. The findings identify specific reading aloud practices, patterns of frequency and perceived barriers to reading aloud in the classroom and at home. The study provides support for the practice of reading aloud to be continued past the period of acquisition and independent reading. It raises concern about the low frequency of reading aloud practices at home and school and the early signs of a literacy. It also highlights the limited attention to affective domains of reading that occur in schools

A ‘near‐life experience’: lived experiences of spirituality from the perspective of people who have been subject to inpatient psychiatric care

Aims: To describe lived experiences of spirituality from the perspective of people who have been subject to inpatient psychiatric care and to interpret these experiences from an understanding of health as dialectical. Methods: After approval from a regional ethical board,eleven participants were recruited from two organisations for people with mental health problems. Participants were asked to narrate about spiritual experiences and occasions where such experiences had come close. The transcribed interviews were analysed by means of a phenomenological hermeneutical approach. Findings: A structural analysis of the text resulted in three themes; perceiving the presence of something extra mundane, making sense of reality and struggling for acceptance. The comprehensive understanding highlights spiritual experiences as going beyond religion, even though religious experiences appear as part of it. These experiences can indeed be a resource contributing to experiences of hope, connectedness, meaning and coherence in life. However, they can also give rise to doubt, anxiety and feelings of loneliness and hopelessness. Rather than understanding spiritual experiences as being either ‘good’ or ‘bad’, we could approach spirituality as something that is always present in alternate and inter-related forms. Metaphorically, this could be understood as a ‘near-life experience’, summarising participants’experiences related to their struggle with issues related to suffering and health which are simultaneously present. Conclusions: If psychiatric nurses could approach this complexity and, without being judgemental, explore seemingly positive and negative experiences of spirituality as dialectically related to each other, rather than viewing them as either resources or problems, this could contribute to insiderness care and hopefully also support people who struggle with these experiences to seek help when needed

Comparison of Bacterial Diversity within the Coral Reef Sponge, Axinella corrugata, and the Encrusting Coral Erythropodium caribaeorum

We compared the Caribbean reef sponge, Axinella corrugata, with the Caribbean reef coral, Erythropodium caribaeorum for differences in their resident microbial communities. This cursory survey of bacterial diversity applied 16S rRNA gene sequences. Over 100 culture-independent sequences were generated from five different Axinella 16S rRNA libraries, and compared with 69 cultured isolates. The cultureindependent 16S rDNA clones displayed a higher diversity of Proteobacteria, including “uncultured” or “unknown” representatives from the Deltaproteobacteria. Arcobacterium, and Cyanobacteria were also found. We have also confirmed that Axinella sponges appeared to host specific microbial symbionts, similar to the previously identified clones termed “OSO” environmental samples. In contrast, seawater samples near Axinella were dominated by Pseudoalteromonas. Adjacent sediment samples yielded clones of Planctomycetacea, Proteobacteria, sulfate-reducing Desulfovibrio spp, and other Deltaproteobacteria. Anaerobe-like 16S rRNA sequences were detected after the oxygen supply to one Axinella sample was deliberately curtailed to assess temporal changes in the microbial community. E. caribaeorum yielded more Betaproteobacteria relative to Axinella 16S libraries, and also included the Gammaproteobacteria genus Spongiobacter. However, Axinella-derived microbes appeared phylogenetically deeper with greater sequence divergences than the coral. Overall this study indicated that marine microbial community diversity can be linked to specific source hosts and habitats

Suppression Subtractive Hybridization PCR Isolation of cDNAs from a Caribbean Soft Coral

Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non- housekeeping genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila Big Brother), catalases, reverse transcriptases, ferritins and various hypothetical protein sequences. A total of 591 EST sequences were deposited into GenBank [dbEST: FL512138 - FL512331, and GH611838]. The results represent proof of concept for enrichment of unique transcripts over housekeeping genes, such as actin or ribosomal genes, which comprised approximately 17% of the total dataset. Due to the gene and sequence diversity of some ESTs, such sequences can find utility as molecular markers in current and future studies of this species and other soft coral biogeography, chemical ecology, phylogenetics, and evolution